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Sukki Kang,석차옥,Juyong Lee,Myeong Sup Lee 대한화학회 2008 Bulletin of the Korean Chemical Society Vol.29 No.2
FLOWERING LOCUS T (FT) and TERMINAL FLOWER 1 (TFL1) in Arabidopsis are homologous proteins that perform opposite functions: FT is an activator of flowering, and TFL1 is a repressor. It was shown before that change of a single amino acid (His88) of TFL1 to the corresponding amino acid (Tyr) of FT is enough to convert the floral repressor to an activator. However, structural basis of the functional conversion has not been understood. In our molecular dynamics simulations on modified TFL1 proteins, a hydrogen bond present in native TFL1 between the His88 residue and a residue (Asp144) in a neighboring external loop became broken by change of His88 to Tyr. This breakage induced conformational change of the external loop whose structure was previously reported to be another key functional determinant. These findings reveal that the two important factors determining the functional specificities of the floral regulators, the key amino acid (His88) and the external loop, are correlated, and the key amino acid determines the functional specificity indirectly by affecting the conformation of the external loop.
Kang, Yeon-koo,Song, Yoo Sung,Cho, Sukki,Jheon, Sanghoon,Lee, Won Woo,Kim, Kwhanmien,Kim, Sang Eun Elsevier 2018 Lung cancer Vol.119 No.-
<P><B>Abstract</B></P> <P><B>Purpose</B></P> <P>In the management of non-small cell lung cancer (NSCLC), the prognostic stratification of stage I tumors without indication of adjuvant therapy, remains to be elucidated in order to better select patients who can benefit from additional therapies. We aimed to stratify the prognosis of patients with stage I NSCLC adenocarcinoma using clinicopathologic factors and F-18 FDG PET.</P> <P><B>Materials and methods</B></P> <P>We retrospectively enrolled 128 patients with stage I NSCLC without any high-risk factors, who underwent curative surgical resection without adjuvant therapies. Preoperative clinical and postoperative pathologic factors were evaluated by medical record review. Standardized uptake value corrected with lean body mass (SUL<SUB>max</SUB>) was measured on F-18 FDG PET. Among the factors, independent predictors for recurrence-free survival (RFS) were selected using univariate and stepwise multivariate survival analyses. A prognostic stratification model for RFS was designed using the selected factors.</P> <P><B>Results</B></P> <P>Tumors recurred in nineteen patients (14.8%). Among the investigated clinicopathologic and FDG PET factors, SUL<SUB>max</SUB> on PET and spread through air spaces (STAS) on pathologic review were determined to be independent prognostic factors for RFS. A prognostic model was designed using these two factors in the following manner: (1) Low-risk: SUL<SUB>max</SUB> ≤ 1.9 and no STAS, (2) intermediate-risk: neither low-risk nor high-risk, (3) high-risk: SUL<SUB>max></SUB>1.9 and observed STAS. This model exhibited significant predictive power for RFS.</P> <P><B>Conclusion</B></P> <P>We showed that FDG uptake and STAS are significant prognostic markers in stage I NSCLC adenocarcinoma treated with surgical resection without adjuvant therapies.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Stage I non-small cell lung cancer (NSCLC) patients recur not infrequently. </LI> <LI> There are no established guidelines that stratify the prognosis of stage I NSCLC. </LI> <LI> F-18 FDG PET/CT and spread through air spaces are independent prognostic factors. </LI> </UL> </P>
Polymorphisms in cancer-related pathway genes and lung cancer
Lee, Shin Yup,Kang, Hyo-Gyoung,Choi, Jin Eun,Jung, Deuk Kju,Lee, Won Kee,Lee, Hyun Chul,Lee, So Yeon,Yoo, Seung Soo,Lee, Jaehee,Seok, Yangki,Lee, Eung Bae,Cha, Seung Ick,Cho, Sukki,Kim, Chang Ho,Lee, European Respiratory Society 2016 The European respiratory journal Vol.48 No.4
<P>We evaluated the associations between potentially functional variants in a comprehensive list of cancer-related genes and lung cancer in a Korean population.</P><P>A total of 1969 potentially functional single nucleotide polymorphisms (SNPs) of 1151 genes involved in carcinogenesis were evaluated using an Affymetrix custom-made GeneChip in 610 nonsmall cell lung cancer patients and 610 healthy controls. A replication study was conducted in an independent set of 490 cases and 486 controls. 68 SNPs were significantly associated with lung cancer in the discovery set and tested for replication.</P><P>Among the 68 SNPs, three SNPs (corepressor interacting with RBPJ 1 (<I>CIR1</I>) rs13009079T>C, ribonucleotide reductase M1 (<I>RRM1</I>) rs1465952T>C and solute carrier family 38, member 4 (<I>SLC38A4</I>) rs2429467C>T) consistantly showed significant associations with lung cancer in the replication study. In combined analysis, adjusted odds ratio for <I>CIR1</I> rs13009079T>C, <I>RRM1</I> rs1465952T>C and <I>SLC38A4</I> rs2429467C>T were 0.69, 0.71 and 0.73, respectively (p=4×10<SUP>−5</SUP>, 0.01 and 0.001, respectively) under the dominant model. The relative mRNA expression level of <I>CIR1</I> was significantly associated with rs13009079T>C genotypes in normal lung tissues (ptrend=0.03).</P><P>These results suggest that the three SNPs, particularly <I>CIR1</I> rs13009079T>C, may play a role in the pathogenesis of lung cancer.</P>
효모의 5.8S rRNA / 26S rRNA 복합체와 E . coli 의 EL24 리보솜 단백질과의 상호작용
강석기,정연희,조봉래,이영훈,박인원 ( Sukki Kang,Yeonhee Joung,Bongrae Cho,Young Hoon Lee,In Won Park ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.6
We here report our preliminary observation that a ribosomal protein EL24 of E. coli participates in the formation of yeast 5.8S rRNA/26S rRNA complex in vitro, and that it is bound to the complex. From this finding we should infer that there exists in yeast ribosome a conserved homolog of EL24, playing a role in the initiation of self-assembly of the ribosome.
Dual roles of a variable number of tandem repeat polymorphism in the TERT gene in lung cancer
Jin, Guang,Yoo, Seung Soo,Cho, Sukki,Jeon, Hyo-Sung,Lee, Won-Kee,Kang, Hyo-Gyoung,Choi, Yi Young,Choi, Jin Eun,Cha, Sung-Ick,Lee, Eung Bae,Kim, Chang Ho,Jung, Tae Hoon,Kim, Young Tae,Park, Jae Yong Wiley (Blackwell Publishing) 2011 CANCER SCIENCE Vol.102 No.1
효모의 5.8S rRNA/26S rRNA 복합체의 구조
강석기,정연희,조봉래,이영훈,박인원 ( Sukki Kang,Yeon Hee Jeong,Bongrae Cho,Young Hoon Lee,In Won Park ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.6
We report the secondary structure model for 5.8S rRNA moiety of 5.8S rRNA/26S rRNA complex in yeast Saccharomyces cerevisiae. The model was inferred from the nuclease S1 and ribonuclease V1 cleavage of 5.8S rRNA. The 5` terminal sequence(nucleotides 4∼32) and 3` terminal sequence(nucleotides 139∼155) of 5.8S rRNA form four helical stems by interacting with domain I of 26S rRNA. The remaining central region, nucleotides 34∼137, form five helical stems and five loops by interacting between its own complementary segments.
Hong, Mi Jeong,Lee, Shin Yup,Choi, Jin Eun,Kang, Hyo‐,Gyoung,Do, Sook Kyung,Lee, Jang Hyuck,Yoo, Seung Soo,Lee, Eung Bae,Seok, Yangki,Cho, Sukki,Jheon, Sanghoon,Lee, Jaehee,Cha, Seung Ick,Kim, C Wiley-Blackwells 2018 Thoracic cancer Vol.9 No.8
<P><B>Background</B></P><P>Genome‐wide association studies have indicated that most of the currently identified disease and trait‐associated single nucleotide polymorphisms (SNPs) are intronic or intergenic. RegulomeDB is a recently developed database that provides functional annotations for regulatory features of SNPs located in non‐coding regions. We evaluated the potential regulatory SNPs in the <I>EGFR</I> gene region using RegulomeDB and their associations with prognosis after surgery in non‐small cell lung cancer (NSCLC) patients.</P><P><B>Methods</B></P><P>A total of 698 patients with surgically resected NSCLC were enrolled and seven SNPs were selected based on the RegulomeDB database. All SNPs were genotyped using SEQUENOM MassARRAY iPLEX assay.</P><P><B>Results</B></P><P>Among the seven SNPs evaluated, rs9642391 (<I>EGFR</I> ivs19+2851C>G) was significantly associated with survival outcome (adjusted hazard ratio [HR] for overall survival = 0.70, 95% confidence interval [CI] 0.56–0.87, <I>P</I> = 0.001; adjusted HR for disease‐free survival = 0.82, 95% CI 0.70–0.97, <I>P</I> = 0.02; under a codominant model). According to RegulomeDB, rs9642391C>G, which is located in intron 19 of <I>EGFR</I>, was predicted to influence the expression of <I>GBAS</I> but not <I>EGFR</I>. As predicted, rs9642391C>G was associated with <I>GBAS</I> (<I>P</I> = 0.024) but not <I>EGFR</I> messenger RNA expression in tumor tissues.</P><P><B>Conclusion</B></P><P>In conclusion, our study provides evidence that rs9642391C>G in the intron of <I>EGFR</I> is associated with <I>GBAS</I> expression and survival outcomes of patients with surgically resected early‐stage NSCLC.</P>