Suppression of AIMP1 protects cognition in Alzheimer’s disease model mice 3xTg-AD

Jang, Sooah,Lee, Jung Ho,Sohn, Bo Kyung,Kim, Eosu,Park, Sang Gyu,Yoon, Kang Jun,Park, Minsun,Kim, Eun Woo,Jeong, Jihyeon,Lee, Jun-Young,Kim, Chul Hoon,Namkoong, Kee Wolters Kluwer Health | Lippincott Williams Wilkin 2017 NEUROREPORT - Vol.28 No.2

<P>Neuroinflammation has been raised as a candidate of unifying pathogenesis and a target of a disease-modifying strategy for Alzheimer's disease (AD). Aminoacyl-tRNA synthetase complex (ARS)-interacting multifunctional protein 1 (AIMP1) is a cytokine that is known to amplify the actions of tumor necrosis factor-a and to be involved in microglial activation and neuronal death. In this respect, AIMP1 could be a plausible target for the treatment of AD. Therefore, we aimed to examine whether anti-AIMP1 antibody could exert therapeutic effects against cognitive impairment using 3xTg-AD mice. Through the passive avoidance test, we found that an intraperitoneal injection of anti-AIMP1 antibody over 4 weeks was effective in protecting memory function in 3xTg-AD mice (16 weeks old). In addition, to address the translational implications of AIMP1, we measured blood AIMP1 levels in patients with AD (n=22), mild cognitive impairment (n=25), and normal cognition (n=23). Blood AIMP1 levels were associated negatively with global cognitive function and were significantly higher in individuals with a higher degree of medial temporal lobe atrophy, which is one of the representative clinical markers of AD. Our results suggested a possible association of AIMP1 with AD pathogenesis, as well as the potential of the anti-AIMP1 antibody as a novel therapeutic option for AD. Copyright (C) 2017 Wolters Kluwer Health, Inc. All rights reserved.</P>

Immune responses of the foot-and-mouth disease virus type Asia1 chimeric vaccine against Asia1 Shamir and Asia1/MOG/05 strains in pigs

Jae Young Kim,Sun Young Park,Gyeongmin Lee,Sang Hyun Park,Jong-Sook Jin,Dohyun Kim,SooAh Lee,Jong-Hyeon Park,Young-Joon Ko 한국예방수의학회 2023 예방수의학회지 Vol.47 No.4

Foot-and-mouth disease (FMD), which affects cloven-hoofed animals, is economically important because of its highly contagious nature. FMD virus (FMDV), the causative agent of FMD, involves seven serotypes (O, A, Asia1, C, and SAT 1-3). Serotype Asia1 is unique to the Asian territory and is subdivided into nine genetic groups (G-I-IX) based on nucleotide variations in the VP1 sequence. Asia1 Shamir, the most representative Asia1 vaccine, is not highly protective against the Asia1/MOG/05 (G-V) lineage found in North Korea in 2007. Therefore, we investigated whether a chimeric virus strain (Asia1/MOG/Shamir), in which the VP4, VP2, and VP3 sequences of Asia1/MOG/05 were combined with the VP1 sequence of Asia1 Shamir, can simultaneously protect against both viruses. We determined the optimal viral growth conditions for the commercial utilization of this chimeric virus strain. Of the three types of cell culture media, the Cellvento medium resulted in the highest amount of antigen in the samples. The chimeric strain was proliferated in a small bioreactor to produce a test vaccine, and its immunogenicity was evaluated in pigs. The virus neutralization (VN) titer against the Asia1 Shamir virus was > 1/100 after the second immunization with the chimeric vaccine in pigs. In addition, a single dose of the test vaccine resulted in a VN titer of > 1/100 against the Asia1/MOG/05 strain. Taken together, our chimeric vaccine strain provided sufficient protection against the Asia1/MOG/05 and Asia1 Shamir viruses, suggesting its potential as a novel vaccine for both these strains.

Copycat Suicide Induced by Entertainment Celebrity Suicides in South Korea

SooAh Jang,JiMin Sung,JinYoung Park,WooTaek Jeon 대한신경정신의학회 2016 PSYCHIATRY INVESTIGATION Vol.13 No.1

Objective-Throughout the past several years, there have been a number of entertainment celebrity suicides in South Korea. The aim of this study was to investigate the clustering of suicides following celebrities’ suicides in South Korea from 2005 to 2008, particularly according to certain characteristics. Methods-Seven celebrity suicides were examined and defined using the Korean Integrated Newspaper Database System (KINDS) and from these, we considered four affected periods occurring 28 days after each celebrity’s suicide. A Poisson time-series autoregression model was used to estimate the relative risk of the total suicide number for each affected period from 2005 to 2008. Logistic regression analysis was performed to investigate whether there were specific increases in the numbers of suicides in subgroups matching each celebrity. Results-There were significant increases in the risk of suicide during the affected periods. Remarkable increases were found in the subgroups matching each celebrity, especially in the group in which all factors (sex, age, and method) were similar. Conclusion-This study provides confirmation that a significant copycat effect was induced by these celebrities’ suicides, especially among people who identified more with the celebrities. This implies that countermeasures for upright media coverage of celebrity suicides should be discussed and practiced properly in South Korea.

Evaluation and Optimization of Metabolome Sample Preparation Methods for Saccharomyces cerevisiae

Kim, Sooah,Lee, Do Yup,Wohlgemuth, Gert,Park, Hyong Seok,Fiehn, Oliver,Kim, Kyoung Heon American Chemical Society 2013 ANALYTICAL CHEMISTRY - Vol.85 No.4

<P>Metabolome sampling is one of the most important factors that determine the quality of metabolomics data. The main steps in metabolite sample preparation include quenching and metabolite extraction. Quenching with 60% (v/v) cold methanol at −40 °C has been most commonly used for Saccharomyces cerevisiae, and this method was recently modified as “leakage-free cold methanol quenching” using pure methanol at −40 °C. Boiling ethanol (75%, v/v) and cold pure methanol are the most widely used extraction solvents for S. cerevisiae. In the present study, metabolome sampling protocols, including the above methods, were evaluated by analyzing 110 identified intracellular metabolites of S. cerevisiae using gas chromatography/time-of-flight mass spectrometry. According to our results, fast filtration followed by washing with an appropriate volume of water can minimize the metabolite loss due to cell leakage as well as the contamination by extracellular metabolites. For metabolite extraction, acetonitrile/water mixture (1:1, v/v) at −20 °C was the most effective. These results imply that the systematic evaluation of existing methods and the development of customized methods for each microorganism are critical for metabolome sample preparation to facilitate the reliable and accurate analysis of metabolome.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2013/ancham.2013.85.issue-4/ac302881e/production/images/medium/ac-2012-02881e_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac302881e'>ACS Electronic Supporting Info</A></P>

Antioxidant Activity of Sargassum fulvellum and Fermentation by Lactobacillus plantarum and Lactobacillus casei

Chaewon Park,Dahee Kim,Sooah Jeong,Beongou Lim 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Sargassum fulvellum (SF) is a marine plant that grows in Southeast Asia and is known to have various physiologically active effects such as antioxidant, anti-inflammatory. Many natural product are reported to have increased antioxidant activity due to the fermentation of lactobacillus. Therefore, we investigated that the antioxidant activity of SF and two species of Lactobacillus fermentation extracts. Two species of Lactobacillus used for fermentation were Lactobacillus plantarum (SFP) and Lactobacillus casei (SFC), each was fermented for 24 hours and then extracted with 70% ethanol for 24 hours, 3 times. The extracts were used after filtration and concentration, and then freeze-dried. Antioxidant activity was evaluated through DPPH radical scavenging, ABTS radical scavenging, Total polyphenol and flavonoid contents, Reducing power, and DNA break repair experiments. As a result, All samples showed increased antioxidant activity in a concentration-dependent manner. From the above results, the fermented SF extract has improved antioxidant effects than SF. We suggest that the fermanted SF will become a strong natural antioxidant drug.

Elucidation of ethanol tolerance mechanisms in <i>Saccharomyces cerevisiae</i> by global metabolite profiling

Kim, Sooah,Kim, Jungyeon,Song, Ju Hwan,Jung, Young Hoon,Choi, Il‐,Sup,Choi, Wonja,Park, Yong‐,Cheol,Seo, Jin‐,Ho,Kim, Kyoung Heon WILEY‐VCH Verlag 2016 BIOTECHNOLOGY JOURNAL Vol.11 No.9

<P><B>Abstract</B></P><P>Ethanol, the major fermentation product of yeast, is a stress factor in yeast. We previously constructed an ethanol‐tolerant mutant yeast iETS3 by using the global transcriptional machinery engineering. However, the ethanol‐tolerance mechanism has not been systematically investigated. In this study, global metabolite profiling was carried out, mainly by gas chromatography/time‐of‐flight mass spectrometry (GC/TOF MS), to investigate the mechanisms of ethanol tolerance in iETS3. A total of 108 intracellular metabolites were identified by GC/TOF MS and high performance liquid chromatography, and these metabolites were mostly intermediates of the central carbon metabolism. The metabolite profiles of iETS3 and BY4741, cultured with or without ethanol, were significantly different based on principal component and hierarchical clustering analyses. Our metabolomic analyses identified the compositional changes in cell membranes and the activation of glutamate metabolism and the trehalose synthetic pathway as the possible mechanisms for the ethanol tolerance. These metabolic traits can be considered possible targets for further improvement of ethanol tolerance in the mutant. For example, the <I>KGD1</I> deletion mutant, with up‐regulated glutamate metabolism, showed increased tolerance to ethanol. This study has demonstrated that metabolomics can be a useful tool for strain improvement and phenotypic analysis of microorganisms under stress.</P>

Anti-inflammatory Efficacy of Fermented Artemisia capillaris Thunb. with Lactococcus lactis, Streptococcus thermophilus and Lactobacillus delbrueckii

Dahee Kim,Sooah Jeong,Chaewon Park,Huijeong Jeong,Beong Ou Lim 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Artemisia capillaris Thunb. (ACT) is a plant of the sagebrush family and is known as a highly self-sustaining plant. It has been used as a medicine since a long time ago, and it is so effective that it is used as a treatment in many places. It helps skin elasticity, is effective in dieting, and helps to inflammation. ACT was extracted through hot water extraction method. ACT was fermented at 37°C for 24 hours using Lactococcus lactis, Streptococcus thermophilus and Lactobacillus delbrueckii. Then, the antioxidant activity was measured using the DPPH and ABTS assays. Samples of fermented ACT were treated in RAW 264.7 cells for 18 hours and then an experiment to measure cellular viability and NO production was conducted. Additionally, western blot and RT-PCR were performed to determine the expression level of anti-inflammatory-related proteins. As a result, it was verified that the fermented ACT has an antioxidant and anti- inflammatory effect. From the above results, we may suggest that fermented ACT has Antioxidant and anti-inflammatory properties. It can be used not only as a medicinal herb, but also as part of a functional food.

Aqueous ammonia pretreatment, saccharification, and fermentation evaluation of oil palm fronds for ethanol production.

Jung, Young Hoon,Kim, Sooah,Yang, Taek Ho,Lee, Hee Jong,Seung, Doyoung,Park, Yong-Cheol,Seo, Jin-Ho,Choi, In-Geol,Kim, Kyoung Heon Springer-Verlag 2012 BIOPROCESS AND BIOSYSTEMS ENGINEERING Vol.35 No.9

<P>Oil palm fronds are the most abundant lignocellulosic biomass in Malaysia. In this study, fronds were tested as the potential renewable biomass for ethanol production. The soaking in aqueous ammonia pretreatment was applied, and the fermentability of pretreated fronds was evaluated using simultaneous saccharification and fermentation. The optimal pretreatment conditions were 7 % (w/w) ammonia, 80 C, 20 h of pretreatment, and 1:12 S/L ratio, where the enzymatic digestibility was 41.4 % with cellulase of 60 FPU/g-glucan. When increasing the cellulase loading in the hydrolysis of pretreated fronds, the enzymatic digestibility increased until the enzyme loading reached 60 FPU/g-glucan. With 3 % glucan loading in the SSF of pretreated fronds, the ethanol concentration and yield based on the theoretical maximum after 12 and 48 h of the SSF were 7.5 and 9.7 g/L and 43.8 and 56.8 %, respectively. The ethanol productivities found at 12 and 24 h from pretreated fronds were 0.62 and 0.36 g/L/h, respectively.</P>

클라우드 문서저작도구의 이용행태 및 개선방향 연구

서보배(Bobae Seo),기수아(Sooah Key),김슬기(Seulkee Kim),박가영(Kayoung Park),이혜민(Hyemin Lee),반영환(Younghwan Pan) 한국HCI학회 2016 한국HCI학회 학술대회 Vol.2016 No.1

Quantification and visualization of metastatic lung tumors in mice

Lee Ha Neul,Kim Seyl,Park Sooah,Jung Woonggyu,Kang Jin Seok 한국독성학회 2022 Toxicological Research Vol.38 No.4

Histopathological examination is important for the diagnosis of various diseases. Conventional histopathology provides a two-dimensional view of the tissues, and requires the tissue to be extracted, fixed, and processed using histotechnology techniques. However, there is an increasing need for three-dimensional (3D) images of structures in biomedical research. The objective of this study was to develop reliable, objective tools for visualizing and quantifying metastatic tumors in mouse lung using micro-computed tomography (micro-CT), optical coherence tomography (OCT), and field emission-scanning electron microscopy (FE-SEM). Melanoma cells were intravenously injected into the tail vein of 8-week-old C57BL/6 mice. The mice were euthanized at 2 or 4 weeks after injection. Lungs were fixed and examined by micro-CT, OCT, FE-SEM, and histopathological observation. Micro-CT clearly distinguished between tumor and normal cells in surface and deep lesions, thereby allowing 3D quantification of the tumor volume. OCT showed a clear difference between the tumor and surrounding normal tissues. FE-SEM clearly showed round tumor cells, mainly located in the alveolar wall and growing inside the alveoli. Therefore, whole-tumor 3D imaging successfully visualized the metastatic tumor and quantified its volume. This promising approach will allow for fast and label-free 3D phenotyping of diverse tissue structures.