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So‑Ra Han,Sung‑Min Jang,Young Min Chi,Byeollee Kim,정상희,Yung Mi Lee,Jun Uetake,Jun Hyuck Lee,Hyun Park,오태진 한국유전학회 2020 Genes & Genomics Vol.42 No.9
Background Spingobium sp. PAMC 28499 is isolated from the glaciers of Uganda. Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor. The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry. Objective In this study, complete genome sequence of Spingobium sp. PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region. Methods Genome sequencingwas performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology. The predicted gene sequences were functionally annotated and gene prediction was carried out using the program NCBI nonredundant database. And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes). Results The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons. Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases. CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae. These results confrmed that this Sphingobium sp. strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway. Conclusion In this study, isolated and sequenced the complete genome of Spingobium sp. PAMC 28499. Also, this strain has comparative genome analysis. Through the complete genome we can predict how this strain can store and produce energy in extreme environment. It can also provide bioengineered data by fnding new genes that degradation the pectin
Assessing Vascular Changes Associated with Healthy Aging using 7T Magnetic Resonance Angiography
So-Ra Jang,Chan-A Park,Kyung-Jin Lee,Yeong-Bae Lee,Hang-Keun Kim,Chang-Ki Kang 한국자기학회 2018 Journal of Magnetics Vol.23 No.1
Aging is known to produce changes in the cerebral vasculature and circulation. We aimed to quantitatively assess age-related changes in the morphology of small perforating vessels, lenticulostriate arteries (LSAs). LSA images were acquired using ultra-high field 7-Tesla magnetic resonance imaging (7T MRI) from 24 young healthy volunteers (young group: YG) and 25 old healthy volunteers (old group: OG). Vessel-related factors such as curvature and tortuosity were analyzed using two-dimensional images. Our quantitative results demonstrated a fewer in their branches and a significantly more pronounced curvature in the OG compared to the YG. These findings were further confirmed qualitatively using image analysis. Our study findings show that MR angiography utilizing ultra-high field MRI can provide high-resolution images that can identify morphological characteristics of small perforating vessels. Based on these features, it is possible to document ageinduced changes in cerebral small vessels.
Development and Evaluation of the Interferon Gamma Test for Detecting Bovine Tuberculosis in Korea
So yoon Ryoo,Yun ho Jang,Na rae Kim,Yoon ra Jang,Doan Lan Pham,Shin seok Kang,Hyeon Seop Byeon,So young Park,Woong seog Song,Suk Chan Jung,In Heo,Bong Kwan Choi,Jae Myung Kim 대한수의학회 2013 대한수의학회 학술대회발표집 Vol.2013 No.-
RCP 기후변화 시나리오에 따른 임하댐 유역의 미래 수문순환 전망
장선숙 ( Sun Sook Jang ),안소라 ( So Ra Ahn ),조형경 ( Hyung Kyung Joh ),김성준 ( Seong Joon Kim ) 한국지리정보학회 2015 한국지리정보학회지 Vol.18 No.1
본 연구는 SWAT(Soil and Water Assessment Tool) 모형을 이용하여 임하댐 유역(1,355.5㎢)을 대상으로 RCP(AR5) 기후변화 시나리오에 따른 미래 수문순환 영향을 평가하였다. 3지점의 실측된 유출량을 활용하여 모형의 보정(2002∼2007년) 및 검증(2008∼2013년)을 실시하였다. 검·보정 결과 결정계수(R2)는 0.70~0.85로, Nash-Sutcliffe 모형 효율(NSE)은 0.67~0.82로 분석되어 신뢰성 있는 유출량 모의 결과를 나타내었다. 기후변화 시나리오는 기상청에서 제공하는 HadGEM3-RA 모형의 RCP 4.5 및 8.5 시나리오를 수집하여, 과거 34년(1980-2013, baseline period)의 기상자료를 기준으로 편이보정(Bias Correction)하여 SWAT 모형에 적용하였다. 기후변화 분석 결과 강수량과 평균기온이 10.8%, 4.9℃ 증가하였으며, 강수량과 기온의 증가로 증발산 11.2%, 토양수분 1.9%, 지표유출 10.0%, 중간유출 12.1%, 회귀유출 18.2%이 각각 증가함에 따라 총 하천유출량이 11.2% 증가하였다. This study was to evaluate the RCP climate change impact on hydrological components in the Imha-Dam watershed using SWAT(Soil and Water Assessment Tool) Model. The model was calibrated for six year(2002∼2007) and validated for six year(2008∼2013) using daily observed streamflow data at three watershed stations. The overall simulation results for the total released volume at this point appear reasonable by showing that coefficient of determination(R2) were 0.70~0.85 and Nash-Sutcliffe model efficiency (NSE) were 0.67-0.82 for streamflow, respectively. For future hydrologic evaluation, the HadGEM3-RA climate data by scenarios of Representative Concentration Pathway(RCP) 4.5 and 8.5 of the Korea Meteorological Administration were adopted. The biased future data were corrected using 34 years(1980~2013, baseline period) of weather data. Precipitation and temperature showed increase of 10.8% and 4.9%, respectively based on the baseline data. The impacts of future climate change on the evapotranspiration, soil moisture, surface runoff, lateral flow, return flow and streamflow showed changes of +11.2%, +1.9%, +10.0%, +12.1%, +18.2%, and +11.2%, respectively.
양성자 및 감마선 처리가 유채 M₁ 세대의 감수성에 미치는 영향
은종선(Jong-Seon Eun),김준수(Jun-Su Kim),임환수(Hwan-Su Lim),한석교(Suk-Kyo Han),최소라(So-Ra Choi),장영석(Young-Seok Jang) 한국원예학회 2007 원예과학기술지 Vol.25 No.1
유채의 돌연변이 육종을 통한 조숙, 단간, 내한성, 내염성 등 농경상 유용형질을 개량하여 재배면적을 증대하고 biodiesel을 개발하고자 품종 ‘내한’, ‘탐미’, ‘한라’ 등의 종자에 양성자와 감마선을 0~2,000Gy 수준으로 처리하여 M1 세대의 방사선 감수성을 조사하였다. 발아율은 품종, 선종 및 선량에 상관없이 95.3% 이상이었고, 파종 6일 후 출현율은 선량이 증가할수록 유의적 감소를 보였으나 파종 12일 후에는 91.7% 이상 출현하여 비슷하였다. 파종 7주일 후 생존율은 1,000Gy까지는 비슷하였으나 1,500Gy와 2,000Gy에서는 38.1~84.1%로 현저하게 감소하였다. 파종 3주일 후 자엽장, 자엽면적 및 하배축장은 3품종 모두 양성자와 감마선의 선량 증가에 따라 급격히 감소하여 통계적 유의성이 인정되었으며 ‘내한’, ‘한라’, ‘탐미’ 순으로 예민한 반응을 보였다. 한편 ‘탐미’의 경우 양성자 처리구의 자엽장과 자엽면적에서 100~200Gy 처리구가 무처리구에 비하여 증가되는 hormesis 현상이 나타났다. 파종 1개월 후 엽록소 함량, 본엽수, 엽면적에서도 선종에 관계없이 선량 증가에 따라 통계적 유의성을 보였으며, 800Gy 이상 고선량구는 자엽이 거의 황화되었고 특히 ‘탐미’ 품종에서 황화현상이 심했다. 본엽수는 대조구와 저선량구에서 2.3매 내외이었으나 1,500Gy 이상 고선량구는 자엽만 전개되었을 뿐 본엽이 거의 생장하지 않았다. 대조구 엽면적의 50% 이상 감소선량은 양성자 처리구에서는 ‘내한’이 800Gy, ‘탐미’와 ‘한라’는 1,500Gy로 나타났고, 감마선 처리구는 ‘탐미’ 400Gy, ‘내한’과 ‘한라’는 1,500Gy로 나타났다. 지상부보다 지하부 생체중의 감수성이 높았는데 50% 이상 감소 선량은 ‘내한’ 600Gy, ‘탐미’와 ‘한라’는 1,000Gy로 나타났다. 유묘기의 생장반응에 있어서 1,000Gy를 초과한 선량에서 50% 이상 감소하는 매우 높은 감수성을 보여 육종에 효율적인 선량은 1,000Gy 이하로 나타났다. This experiment was carried out to increase the cultivation area of rape (Brassica napus) and develop this plant as a biodiesel so dry seeds of 3 cultivars, ‘Naehan’, ‘Hanla’, and ‘Tammi’ were irradiated with proton ion and gamma-ray at range of 0 to 2,000 Gy and then radiosensitivity of M1 generation were investigated. The germination rate were more than 95.3% regardless of radiation source, dosage and cultivar and emergence rate at 6 days after sowing were decreased by increasing dosage but those of 12 days as more than 91.7% were similar all together. Survival rate in 1,000 Gy and below at 7 weeks after sowing were similar but remarkably reduced 38.1~84.1% in 1,500 Gy and 2,000 Gy. The length and area of cotyledon, and hypocotyl length in all 3 cultivars were badly reduced with high significancy by increasing dosage of proton ion and gamma-ray irradiation and sensitive responses were showed in order of ‘Naehan’, ‘Hanla’, and ‘Tammi’. On the other hand, there was hormesis that the length and area of cotyledon were more increased at 100~200 Gy in the proton ion than control in ‘Tammi’. Chlorophyll content, number of true leaves and leaf area at 1 month after sowing were decreased by increasing radiation dosage regardless of radiation source so had high significancy. As the cotyledons at 1 month after sowing almost showed chlorosis in more than 800 Gy and chlorosis was especially serious in ‘Tammi’. The number of true leaves was about 2.3 in control and the low level of dosage but cotyledon didn’t grow in the high level dosage of 1,500 Gy and over. And the dosage in which leaf area was reduced to 50% compared to that of control were 800 Gy in ‘Naehan’ and 1,500 Gy in ‘Tammi’ and ‘Hanla’ by proton ion and 400 Gy in ‘Tammi’, 1,500 Gy both ‘Naehan’ and ‘Hanla’ by gamma-ray. The fresh weight of top land than root was more sensitive so dosage in which fresh weight reduced to 50% compared to that of control were 600 Gy in ‘Naehan’ and 1,000 Gy both ‘Tammi’ and ‘Hanla’. As the results, growth of seedling decreased over 50% in more than 1,000 Gy with high sensitivity to radiation and so it was appeared that proper dosage for mutation breeding in rape was 1,000 Gy and below.