Immobilization of Lipase using Alginate Hydrogel Beads and Enzymatic Evaluation in Hydrolysis of p-Nitrophenol Butyrate

Zhang, Shuang,Shang, Wenting,Yang, Xiaoxi,Zhang, Shujuan,Zhang, Xiaogang,Chen, Jiawei Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.9

The immobilization of enzyme is one of the key issues both in the field of enzymatic research and industrialization. In this work, we reported a facile method to immobilize Candida Antarctica lipase B (CALB) in alginate carrier. In the presence of calcium cation, the enzyme-alginate suspension could be cross-linked to form beads with porous structure at room temperature, and the enzyme CALB was dispersed in the beads. Activity of the enzyme-alginate composite was verified by enzymatic hydrolysis reaction of p-nitrophenol butyrate in aqueous phase. The effects of reaction parameters such as temperature, pH, embedding and lyophilized time on the reactive behavior were discussed. Reuse cycle experiments for the hydrolysis of p-nitrophenol butyrate demonstrated that activity of the enzyme-alginate composite was maintained without marked deactivation up to 6 repeated cycles.

Characterization of microbiota of naturally fermented sauerkraut by high-throughput sequencing

Shuang Zhang,Yichen Zhang,Lihong Wu,Lili Zhang,Song Wang 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.6

Sauerkraut is a traditionally fermented cabbage, with a unique taste and beneficial properties, in northeast China. The taste and flavor of sauerkraut vary from region to region, owing to the differences in microorganisms. Illumina MiSeq sequencing was used to identify and quantify the microbial community composition of the broth and leaves of the naturally fermented Suan-cai collected from northeast China. The alpha and beta diversity of the samples from three areas in Heilongjiang province shown that the complexity of bacterial diversity of the three samples was C, A and B in turn. The Lactobacillus widely existed in fermented sauerkraut, of these, Latilactobacillus sakei, Loigolactobacillus coryniformis subsp. torquens, Lactiplantibacillus plantarum subsp. plantarum, and Secundilactobacillus malefermentans were more abundant in the sauerkraut leaves than in fermentation broth. Other genera of lactic acid bacteria Pediococcus and Leuconostoc, which have potential probiotic properties, were also present. However, some harmful bacteria such as Arcobacter and Acinetobacter were also detected.

Immobilization of Lipase using Alginate Hydrogel Beads and Enzymatic Evaluation in Hydrolysis of p-Nitrophenol Butyrate

Shuang Zhang,Wenting Shang,Xiaoxi Yang,Shujuan Zhang,Xiaogang Zhang,Jiawei Chen 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.9

The immobilization of enzyme is one of the key issues both in the field of enzymatic research and industrialization. In this work, we reported a facile method to immobilize Candida Antarctica lipase B (CALB) in alginate carrier. In the presence of calcium cation, the enzyme-alginate suspension could be cross-linked to form beads with porous structure at room temperature, and the enzyme CALB was dispersed in the beads. Activity of the enzyme-alginate composite was verified by enzymatic hydrolysis reaction of p-nitrophenol butyrate in aqueous phase. The effects of reaction parameters such as temperature, pH, embedding and lyophilized time on the reactive behavior were discussed. Reuse cycle experiments for the hydrolysis of pnitrophenol butyrate demonstrated that activity of the enzyme-alginate composite was maintained without marked deactivation up to 6 repeated cycles.

Longitudinal Intrinsic Brain Activity Changes in Cirrhotic Patients before and One Month after Liver Transplantation

Yue Cheng,Li-Xiang Huang,Li Zhang,Ming Ma,Shuang-Shuang Xie,Qian Ji,Xiao-Dong Zhang,Gao-Yan Zhang,Xue-Ning Zhang,Hong-Yan Ni,Wen Shen 대한영상의학회 2017 Korean Journal of Radiology Vol.18 No.2

Objective: To evaluate the spontaneous brain activity alterations in liver transplantation (LT) recipients using resting-state functional MRI. Materials and Methods: Twenty cirrhotic patients as transplant candidates and 25 healthy controls (HCs) were included in this study. All patients repeated the MRI study one month after LT. Amplitude of low-frequency fluctuation (ALFF) values were compared between cirrhotic patients (both pre- and post-LT) and HCs as well as between the pre- and post-LT groups. The relationship between ALFF changes and venous blood ammonia levels and neuropsychological tests were investigated using Pearson’s correlation analysis. Results: In the cirrhotic patients, decreased ALFF in the vision-related regions (left lingual gyrus and calcarine), sensorimotor-related regions (left postcentral gyrus and middle cingulate cortex), and the default-mode network (bilateral precuneus and left inferior parietal lobule) were restored, and the increased ALFF in the temporal and frontal lobe improved in the early period after LT. The ALFF decreases persisted in the right supplementary motor area, inferior parietal lobule, and calcarine. The ALFF changes in the right precuneus were negatively correlated with changes in number connection test-A scores (r = 0.507, p < 0.05). Conclusion: LT improved spontaneous brain activity and the results for associated cognition tests. However, decreased ALFF in some areas persisted, and new-onset abnormal ALFF were possible, indicating that complete cognitive function recovery may need more time.

A Time-Varying Control Method Based on Taylor Series Approximation

Shuang Zhang,Zuojun Shen 제어로봇시스템학회 2015 제어로봇시스템학회 국제학술대회 논문집 Vol.2015 No.10

In this paper, a time-varying control method based on Taylor series approximation is proposed. By approximation, a time-varying system can be explicated as a polynomial function of the independent variable, and a time-varying control law can be derived. For a time-varying system, this control law guarantees the actual control result as similar as the promising one of a linear time-invariant control method, such as the proportional feedback scheduling or the gain scheduling. A benchmark control problem of stabilizing an inverted pendulum with an oscillating center of mass is used to illustrate the effectiveness of the new method. Stability analysis is also conducted using the state-of-art singular perturbation margin (SPM) approach.

Modeling and Vibration Control of a Coupled FloatingWind Turbine System

Shuang Zhang,Hui Qin,Xiuyu He,Wei He 제어로봇시스템학회 2015 제어로봇시스템학회 국제학술대회 논문집 Vol.2015 No.10

This paper presents the dynamic modeling and vibration control design of a floating wind turbine system. Two flexible mooring lines attached to a flexible wind turbine tower (taut-moored floating spar) are used to represent the dynamics of system. The wind turbine tower is modeled as an Euler-Bernoulli beam, and the mooring lines are modeled as two strings. The control laws are designed to suppress vibrations of both wind turbine tower and the mooring lines. Based on the construction of a physically motivated Lyapunov function, the bounded stability is proved with the proposed control. Numerical examples illustrate the performance of the control system.

Human Recombinant Endostatin Combined with Cisplatin Based Doublets in Treating Patients with Advanced NSCLC and Evaluation by CT Perfusion Imaging

Zhang, Feng-Lin,Gao, Er-Yun,Shu, Rong-Bao,Wang, Hui,Zhang, Yan,Sun, Peng,Li, Min,Tang, Wei,Jiang, Bang-Qin,Chen, Shuang-Qi,Cui, Fang-Bo Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15

Aims: To study the effectiveness of human recombinant endostatin injection (Endostar(R)) combined with cisplatin doublets in treating advanced non-small cell lung cancer (NSCLC), and to evaluate outcome by CT perfusion imaging. Methods: From April 2011 to September 2014, 76 patients with advanced NSCLC who were treated with platinum-based doublets were divided into group A (36 patients) and group B (40 patients). Endostar(R) 15mg/day was administered 4 days before chemotherapy and combined with chemotherapy from day 5 in group A, and combined with chemotherapy from the first day in Group B. Endostar(R) in the two groups was injected intravenously for 14 days. Results: Treatment effectiveness in the two groups differed with statistical significance (p<0.05). Effectiveness evaluated by CT perfusion imaging, BF, BV, MTT and PS also demonstrated significant differences (all p<0.05). Adverse reactions in the two groups did not significantly vary (p> 0.05). Conclusions: The response rate with Endostar(R) administered 4 days before chemotherapy and combined with chemotherapy from day 5 in group A was better than Endostar(R) combined with chemotherapy from the first day, and CT perfusion imaging could be a reasonable method for evaluation of patient outcomes.

Halomonas alkalitolerans sp. nov., a Novel Moderately Halophilic Bacterium Isolated from Soda Meadow Saline Soil in Daqing, China

Shuang Wang,Qian Yang,Zhi-Hua Liu,Lei Sun,Dan Wei,Jun-Zheng Zhang,Jin-Zhu Song,Yun Wang,Jia Song,Jin-Xia Fan,Xian-Xin Meng,Wei Zhang 한국미생물학회 2011 The journal of microbiology Vol.49 No.1

A moderately halophilic bacterial strain 15-13^T, which was isolated from soda meadow saline soil in Daqing City, Heilongjiang Province, China, was subjected to a polyphasic taxonomic study. The cells of strain 15-13^T were found to be Gram-negative, rod-shaped, and motile. The required growth conditions for strain 15-13^T were: 1-23% NaCl (optimum, 7%), 10-50°C (optimum, 35°C), and pH 7.0-11.0 (optimum, pH 9.5). The predominant cellular fatty acids were C18:1 ω7c (60.48%) and C16:0 (13.96%). The DNA G+C content was 67.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain 15-13^T clustered within a branch comprising species of the genus Halomonas. The closest phylogenetic neighbor of strain 15-13^T was Halomonas pantelleriensis DSM 9661^T (98.9% 16S rRNA gene sequence similarity). The level of DNA-DNA relatedness between the novel isolated strain and H. pantelleriensis DSM 9661^T was 33.8%. On the basis of the phenotypic and phylogenetic data, strain 15-13^T represents a novel species of the genus Halomonas, for which the name Halomonas alkalitolerans sp. nov. is proposed. The type strain for this novel species is 15-13^T (=CGMCC 1.9129^T =NBRC 106539^T).

Research on the Real-time Monitoring System of Cow’s Rumination

Shuang Zhang,Yu Zhang,Weizheng Shen,Lu Xu,Hao Wu,Zhongbin Su 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.7

Cow’s rumination is an important process of food digestion for dairy cows which is a valuable indicator during the cow management. By measuring the cow’s rumination time, it is capable to predict the cow’s estrus, and learn the cow’s health situation. This paper uses MSP430F149 processor with a sound sensor to achieve the design of real-time monitoring system for cow’s rumination. Since the system require high accuracy of the audio signal of the cow’s rumination, the ADC acquisition module and filtering and amplification process was made a special design. For the change of the spectral characteristics of the voice when cows ruminating, this paper designed the endpoint detection algorithm and sound sequence windowed function, and FFT transform is performed on the data in the Hamming window, and then do the frequency domain analysis to the audio of cow’s rumination. Comparing the sound spectrum collected from high precision recording instrument and this system, this system has done a good job in frequency and time domain. Applying the high precision recording instrument to the simulation experiment for cow’s rumination which conduct 50 sets of different time ranging, using this system to do the real-time monitoring, the mean error was 4.38, the R2 value is 0.877, the Pearson correlation was 0.936. This system performed accurate in the acquisition of the sound signal, and more accurately in identifying cows ruminating, and it can also provide effective means for the intelligent management of the pasture on cow’s health and breeding.

Optimized inverse distance weighted interpolation algorithm for γ radiation field reconstruction

Zhang Biao,Cao Jinjia,Lin Shuang,Li Xiaomeng,Zhang Yulong,Zheng Xiaochang,Chen Wei,Song Yingming 한국원자력학회 2024 Nuclear Engineering and Technology Vol.56 No.1

The inversion of radiation field distribution is of great significance in the decommissioning sites of nuclear facilities. However, the radiation fields often contain multiple mixtures of radionuclides, making the inversion extremely difficult and posing a huge challenge. Many radiation field reconstruction methods, such as Kriging algorithm and neural network, can not solve this problem perfectly. To address this issue, this paper proposes an optimized inverse distance weighted (IDW) interpolation algorithm for reconstructing the gamma radiation field. The algorithm corrects the difference between the experimental and simulated scenarios, and the data is preprocessed with normalization to improve accuracy. The experiment involves setting up gamma radiation fields of three Co-60 radioactive sources and verifying them by using the optimized IDW algorithm. The results show that the mean absolute percentage error (MAPE) of the reconstruction result obtained by using the optimized IDW algorithm is 16.0%, which is significantly better than the results obtained by using the Kriging method. Importantly, the optimized IDW algorithm is suitable for radiation scenarios with multiple radioactive sources, providing an effective method for obtaining radiation field distribution in nuclear facility decommissioning engineering.