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Shin, J.I.,Lim, H.Y.,Kim, H.W.,Seung, B.J.,Ju, J.H.,Sur, J.H. ACADEMIC PR 2016 JOURNAL OF COMPARATIVE PATHOLOGY Vol.155 No.1
<P>This study was designed to investigate the role of obesity in canine malignant mammary tumours (CMMTs), by assessing aromatase expression and the regulatory roles of immune mediators such as cyclo-oxygenase-2 (COX2), prostaglandin E-2 (PGE(2)), nuclear factor kappa beta (NF-kappa B), hypoxia inducible factor-1 alpha (HIF-1 alpha cc) and adipokines (i.e. leptin) in lean, optimal body weight, overweight and obese animals. Clinicopathological data, including the breed, body weight, body condition score and age and neutering status, were collected, together with histopathological characteristics (i.e. histological types, grading and lymphatic invasion). To determine the expression of each factor, immunohistochemistry was conducted with 60 samples of malignant CMMTs. CMMTs from overweight and obese animals had significantly elevated levels of PGE(2), and aromatase expression correlated significantly with PGE(2), NF-kappa B and leptin expression. However, no significant difference was observed in terms of histopathological characteristics. The results suggest that PGE(2), a known obesity-related immune mediator, could be upregulated in CMMTs from overweight and obese animals. In addition, PGE(2), NF-kappa B and leptin influenced the expression of aromatase, as observed in women. (C) 2016 Elsevier Ltd. All rights reserved.</P>
Shin, J.I.,Song, K.S.,Kim, H.,Cho, N.H.,Kim, J.,Kim, H.S.,Lee, J.S. Blackwell Publishing Ltd 2011 British journal of dermatology Vol.164 No.6
<P><B>Summary</B></P><P><B>Background </B> Because inflammatory cytokines are known to be potent inducers of matrix metalloproteinases (MMPs), and MMPs themselves can promote inflammation, we speculated that MMP activation might be involved in the pathogenesis of Henoch–Schönlein purpura (HSP) vasculitis.</P><P><B>Objectives </B> To investigate the gene expression profile of all known MMPs and tissue inhibitors of metalloproteinases (TIMPs) in children with HSP and to examine the role, if any, of MMPs in the pathogenesis of HSP.</P><P><B>Methods </B> Peripheral blood samples were obtained from 10 patients with HSP (nine were in the acute stage, one had HSP nephritis) and four healthy controls. Peripheral blood samples were also taken from the nine patients with HSP when they reached the convalescent stage of the disease. From these samples, total RNA was purified and gene expressions were measured using real‐time polymerase chain reaction.</P><P><B>Results </B> MMP‐8 expression was decreased in patients with arthralgia (<I>P </I>=<I> </I>0·038), and MMP‐3 (<I>P </I>=<I> </I>0·03) and TIMP‐4 expressions (<I>P </I>=<I> </I>0·016) were elevated in HSP patients with nephritis. Soft tissue oedema was associated with decreased expressions of MMP‐26 (<I>P </I>=<I> </I>0·038) and MMP‐28 (<I>P </I>=<I> </I>0·038). MMP‐1, MMP‐8, MMP‐9, MMP‐10, MMP‐13, MMP‐16 and MMP‐26 levels were significantly higher in patients in the acute stage of HSP than in normal controls (<I>P </I><<I> </I>0·05). MMP‐9 (<I>P </I>=<I> </I>0·097) and MMP‐19 (<I>P </I>=<I> </I>0·054) levels decreased to borderline significance in patients in the convalescent stage compared with those in the acute stage. The duration of steroid administration was negatively correlated with MMP‐1, MMP‐2, MMP‐7, MMP‐10, MMP‐12, MMP‐19, MMP‐23 and TIMP‐1 levels (<I>P </I><<I> </I>0·05), suggesting a suppressive effect of steroids on the expressions of MMPs and TIMPs.</P><P><B>Conclusions </B> This is the first study to describe the expression profile of all known MMPs and TIMPs in children with HSP, and our results suggested that abnormal levels of MMP and TIMP activity may have a role in the pathogenesis of HSP.</P>
Shin, Ji‐,Ae,Kim, Jae‐,Jin,Choi, Eun‐,Sun,Shim, Jung‐,Hyun,Ryu, Mi Heon,Kwon, Ki Han,Park, Hee‐,Min,Seo, Jin‐,Young,Lee, Soo‐,Yeon,Lim, Do‐,Won,Cho, Nam Wiley Subscription Services, Inc., A Wiley Company 2013 Head & neck Vol.35 No.7
<P><B>Abstract</B></P><P><B>Background</B></P><P>The aims of this study were to evaluate the apoptotic activities and molecular mechanisms of methanol extracts of <I>Dianthus chinensis</I> (MEDC) and <I>Acalypha australis</I> L. (MEAL) in human oral cancer cells.</P><P><B>Methods</B></P><P>The apoptotic effects and related molecular mechanisms of MEDC and MEAL on oral cancer cells were evaluated using MTS assay, DAPI staining, immunostaining, Western blotting, and reverse transcriptase–polymerase chain reaction.</P><P><B>Results</B></P><P>Sp1 was overexpressed in oral tumor tissues compared with normal oral mucosa. Downregulation of Sp1 inhibited the growth of SCC‐15 and YD‐15 oral cancer cells. MEDC and MEAL inhibited cell growth and induced apoptosis in both cell lines by decreasing the expression of Sp1. In addition, treatment of cells with MEDC and MEAL decreased Mcl‐1 expression, which is a downstream target of Sp1.</P><P><B>Conclusion</B></P><P>Our results indicate that MEDC and MEAL are bioactive natural products that can potentially induce apoptosis of tumor cells that overexpress the Sp1 protein. © 2012 Wiley Periodicals, Inc. Head Neck, 2013</P>
Shinji Hiyama,Toshie Sugiyama,Seiji Kusuhara,Takashi Uchida 대한수의학회 2012 JOURNAL OF VETERINARY SCIENCE Vol.13 No.3
The temporal expression of estrogen receptor (ER)α and ERβ mRNA was examined in male Japanese quails. Femurs of quails receiving 17βestradiol underwent RTPCR and histochemical analysis 1 to 15 days after treatment. Untreated quails were used as controls (day 0). Between days 0 and 5, cells lining the bone endosteal surface differentiated into osteoblasts, which in turn formed medullary bone. Expression of ERα was already observed on day 0 and increased slightly during bone formation whereas ERβ was hardly detected throughout this process. After osteoclasts appeared on the medullary bone surface, this type of bone disappeared from the bone marrow cavity (days 7∼15). ERα expression simultaneously decreased slightly and ERβ levels remained very low. These results suggest that estrogen activity mediated by ERα not only affects medullary bone formation but also bone resorption.
Integrated Production Planning and Scheduling Using Backward/Forward Scheduling Simulation
Shinji Hirano,Shigeo Kobayashi,Toru Eguchi 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
This paper deals with release time planning to reduce lead times of jobs in make-to-order productionenvironments. In traditional MRP-based planningsystem, lead times are estimated using historical data and pastexperience without precisely considering capacity constraints.The proposed method in this paper is based on precisebackward and forward scheduling simulation. First, backward simulation in which simulation starts at each due date ofjob and sends it through the shop floor based on the backward sequence of its part routing is applied. Next, forwardsimulation is applied to modify the release times obtained by backward simulation. A genetic algorithm is also used tominimize lead times of jobs in the final step. Numerical experiments of planning and scheduling with processing timeuncertainty are carried out to examine the effectiveness of the proposed method.