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Shazia Perveen,Ji Seon Yang,Tae Joung Ha,Shin Hee Yoon 대한생리학회-대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.4
Flavonoids have an ability to suppress various ion channels. We determined whether one of flavonoids, cyanidin-3-glucoside, affects adenosine 5 -triphosphate (ATP)-induced calcium signaling using digital imaging methods for intracellular free Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]i), reactive oxygen species (ROS) and mitochondrial membrane potential in PC12 cells. Treatment with ATP (100μM) for 90 sec induced [Ca<sup>2+</sup>]i increases in PC12 cells. Pretreatment with cyanidin-3-glucoside (1μg/ml to 100μg/ml) for 30 min inhibited the ATP-induced [Ca<sup>2+</sup>]i increases in a concentration-dependent manner (IC50=15.3μg/ml). Pretreatment with cyanidin-3-glucoside (15μg/ml) for 30 min significantly inhibited the ATP-induced [Ca<sup>2+</sup>]i responses following removal of extracellular Ca<sup>2+</sup> or depletion of intracellular [Ca<sup>2+</sup>]i stores. Cyanidin-3-glucoside also significantly inhibited the relatively specific P2X2 receptor agonist 2-MeSATP-induced [Ca<sup>2+</sup>]i responses. Cyanidin-3-glucoside significantly inhibited the thapsigargin or ATP-induced store-operated calcium entry. Cyanidin-3-glucoside significantly inhibited the ATP-induced [Ca<sup>2+</sup>]i responses in the presence of nimodipine and ω-conotoxin. Cyanidin-3- glucoside also significantly inhibited KCl (50 mM)-induced [Ca<sup>2+</sup>]i increases. Cyanidin-3-glucoside significantly inhibited ATP-induced mitochondrial depolarization. The intracellular Ca<sup>2+</sup> chelator BAPTA-AM or the mitochondrial Ca<sup>2+</sup> uniporter inhibitor RU360 blocked the ATP-induced mito-chondrial depolarization in the presence of cyanidin-3-glucoside. Cyanidin-3-glucoside blocked ATP- induced formation of ROS. BAPTA-AM further decreased the formation of ROS in the presence of cyanidin-3-glucoside. All these results suggest that cyanidin-3-glucoside inhibits ATP-induced calcium signaling in PC12 cells by inhibiting multiple pathways which are the influx of extracellular Ca<sup>2+</sup> through the nimodipine and ω-conotoxin-sensitive and -insensitive pathways and the release of Ca<sup>2+</sup> from intracellular stores. In addition, cyanidin-3-glucoside inhibits ATP-induced formation of ROS by inhibiting Ca<sup>2+</sup>-induced mitochondrial depolarization.
Shazia Quasin,Manju Siliwal,Virendra Prasad Uniyal 국립중앙과학관 2018 Journal of Asia-Pacific Biodiversity Vol.11 No.1
The genus Trachelas is well reported around the world with 86 valid species known so far, of which, only two species have been reported from India. In this article, we describe a new species Trachelas chamoli sp. nov from Uttarakhand, India.
Dynamic Wetting in Microfluidic Droplet Formation
Shazia Bashir,Xavier Casadevall i Solvas,Muhammad Bashir,Julia Margaret Rees,William Bauer Jay Zimmerman 한국바이오칩학회 2014 BioChip Journal Vol.8 No.2
The extent to which the carrier fluid wets the walls of a microchannel is crucial in the droplet formation process for segmented flow microfluidic applications and can be influenced by the use of surfactants. Surfactants dynamically modify the microchannel surface leading to stabilization of the two phase interface, affecting the droplet formation process. An experimental study of the influence of hydrophobic surfactant (Span 80) during the formation of water-inoil droplets in a T-shaped microchannel geometry is presented and the wetting properties of the microchannel walls were characterized. The range of data to be analyzed on the microscale is estimated from the macroscopic interfacial tension and contact angle measurements. The critical micelle concentration (CMC) level at the microscale was estimated by observing the trend of droplet length variation with concentration of surfactant in a microchannel. Microchannels used in this work were fabricated using softlithography methods and bonded using a custom-made plasma bonding setup that does not require an ultra high vacuum chamber and hence saves the fabrication cost.
Fatigue analysis of overlapped tubular joints
Shazia Muzaffer,Kyong-Ho Chang,ZhenMing Wang,Wang-Sub Shin 대한용접·접합학회 2021 대한용접학회 특별강연 및 학술발표대회 개요집 Vol.2021 No.11
The increasing demand of on the use of wind energy during recent years, have led to installation of more durable, safe and stable offshore structures in deep waters. The circular hollow section tubular joints are generally preferred in offshore structures. The overlapped joints are considered better than simple gap K-joints because of their higher axial strength, high stiffness and high durability. These tubular joints being subjected to combination of wind and waves load during their design period, the constantly acting loads deteriorates the structure, which often results in total collapse of offshore structures. In order to allow for cost effective and reliable design, the substantial requirement is to do accurate and reliable simulation. In this study in order to understand the behavior of crack initiation and the fatigue life of overlapped joints, the 3D fatigue FEM analysis of overlapped joint was carried out. The 3D fatigue FEM study based on continuum damage mechanics and constitutive model, was carried out in two steps namely 3D thermo elastic-plastic analysis and 3D non-steady heat conduction analysis. The fatigue life and crack initiation positions were determined. Finally, S-N curves were plotted and the results from FEM analysis were compared with Hot Spot Stress and with S-N curves recommended by Eurocode 3.
Fatigue Analysis of Tripod wind turbine support structure
Shazia Muzaffer,Kyong-Ho Chang(장경호),ZhenMing Wang(왕진명),Wang sub Shin 대한용접·접합학회 2021 대한용접학회 특별강연 및 학술발표대회 개요집 Vol.2021 No.5
The offshore wind turbines in deep waters requires building large foundations, which transfers loads from wind turbine to ground. The tripod being considered the most robust and secure supporting structure due to its good strength and stability. The welded joints in the structure being exposed to different environmental loads such as wave load, ice load, wind load and earthquake for longer periods increases the stress concentration at hotspot positions which in turn leads to crack initiation and ultimately the failure of whole structure. In this work the main objective is to investigate the fatigue life and crack initiation positions of welded joint in Tripod structure by using 3D fatigue FEM analysis. Firstly, in 3-D Fem analysis, welding residual stresses and welding deformation is calculated by employing sequentially coupled 3-D thermo-mechanical analysis. Secondly, a nonlinear damage model based on continuum damage mechanics for multiaxial high cycle fatigue is used to predict the fatigue life and fatigue crack initiation. The results obtained from analysis were quite satisfying, so 3D fatigue FEM can predict the fatigue life of structures with great accuracy.