참외ㆍ멜론의 품종 분류 및 명명에 관한 최근 동향과 분석

이승인(Seung-In Yi),권용삼(Yong-Sham Kwon),배경미(Kyung-Mi Bae),송인호(In-Ho Song) 한국원예학회 2004 원예과학기술지 Vol.22 No.4

참외는 식물분류학상으로는 멜론과 동일하게 Cucumis melo L. (2n=24)에 속한다. 멜론은 전 세계적으로 다양한 지리적 분포와 함께 많은 야생종과 재배종이 알려져 있으며, 아프리카 지역이 종다양성의 지리적 중심을 이루고 있다. 멜론의 순화는 약 4000년전 아시아와 아프리카 지역에서 각각 독립적으로 진행되었을 것으로 추정되었다. 멜론 작물의 형태적 다양성은 변종들 간의 혼종과 타가수정 작물로서 자연교잡에 의하여 주로 진행되었으며, 이로 인하여 국내외적으로 종 분류에 혼란이 상존해 왔다. 우리나라는 오랜 참외 재배역사와 함께 다양한 재래종과 ‘금싸라기’로 대표되는 재배종 품종들을 가지고 있다. 참외를 포함한 멜론의 분류명으로는 cantalupensis, reticulatus, makuwa 등의 변종명이 오랫동안 사용되어져 왔다. 그러나 최근 새로운 분류체계의 성립과 육종 및 분자유전학의 발달에 따라 많은 논문에서는 cantalupensis와 reticulatus의 분류가 통합되어 칭하여지는 등 현재 우리나라에서 사용되어지고 있는 관행들이 일반적으로 준용되는 국제 기준의 분류 및 명명법과 상호 일치되지 않는 부분들이 발견되고 있다. 본 총설에서는 이에 관한 국내외 기준의 통용성을 확보하는 차원에서, 복잡한 양상을 띠고 있는 C. melo 종들의 분류와 명명법 체계에 대한 발전 내용을 중심으로 최근 논의동향을 살펴보았다. Melon (Cucumis melo L., 2n = 24) including oriental melon contains genetically very diverse varieties. Wild and cultivated melon types have been found of different geographical origin and Africa has been proposed as a possible center of the diversity. The domestication of melon might have occurred independently or in parallel in Asia and Africa since nearly 4000 years ago. The high polymorphism of melons has largely derived from the recombinant cultivars between varieties and its open pollination characteristics in nature, which led botanists to propose different infraspecific classifications. Korea has long history of cultivation for oriental melon, with large varieties of landrace and market classes including ‘Geumssaraki’ type. And botanical nomenclature system such as cantalupensis, reticulatus, and makuwa etc. have been used for the variety denomination. But, ever since the introduction of the system, many progresses have been achieved in the field of nomenclature, breeding and molecular genetics, which suggest critical review on the current situation and consideration for establishment of new system. This paper reviews the recent advances related with classification and nomenclature and give some comments for the efficient and scientific description of C. melo species.

종자산업법 제정 이전의 우리나라 종자관리 법령과 제도의 변천

이승인(Seung-in YI),방문진(Munjin Bang) 한국육종학회 2020 한국육종학회지 Vol.52 No.S

The history of development of seed management in Korea can be broadly divided into the periods of pre-enlightenment (Joseon Dynasty), enlightenment, Japanese colonial occupation, after liberation, installation of the National Seed Supply Office, and the Seed Industry Act. During the Joseon Dynasty, quite a few agricultural books had been published. With the signing of the Korea-U.S. Trade Treaty in the late 19th century, the Agriculture and Livestock Experimental Station was established, and subsequently many varieties were introduced from the U.S. and tested in the station. The Agricultural Exemplary Testing Station, founded in Suwon by the Japanese Resident General in 1906 as a national agricultural research facility, was a hub in colonial agricultural research and food production. In order to expand agricultural productivity, the Regulations on Subsidization of Rice Seed Production was enacted in 1922. This carried out nation-wide seed production and supply projects, and established a seed multiplication system. After liberation, the seed management system of food crops was enacted under the Major Crop Seed Act (1962). The Agricultural Seeds and Seedlings Act (1962) was enacted for vegetables and other crops, and then the Seedlings Management Act (1973) replaced it. In 1974, the National Seed Supply Office was established as an institution that supplies high quality seeds for food self-sufficiency with support of the FAO and IBRD. Then, by introducing the variety protection system under the Seed Industry Act (1997), Korea was able to reform the national seed management system and prepare an opportunity to join the ranks of developed countries.

국제 탄소시장의 교토크레딧 수요공급 불균형에 대한 고찰

유인식(In-Sik Yu),이승묵(Seung-Muk Yi),최경식(Keoung-Sik Choi) 한국에너지기후변화학회 2008 에너지기후변화학회지 Vol.3 No.1

With the decision to formally adopt the Bali Action Plan through COP-13, it has become unavoidable that Korea join in and share the responsibilities for reduction in greenhouse gases after 2012 (post Kyoto). Korea is currently participating in the Carbon market assertively through CDM business, and the government is also presenting diverse range of policy means for participation in and support for international carbon market, Although presumption of future carbon market is an important items that needs to be considered at the time of participation of private sector projects and establishment of national policy, researches for such is yet to be carried out due to high level of uncertainties, Items that need to be considered in presuming the demand and supply for emission rights (or reduction rights) of the future carbon market are extremely diverse. In this study, demand for each country within Annex I have been considered from the perspective of demand for presumption. For supply, CERs, ERUs, and AAU data have been utilized. The outcome of analysis illustrate that the annual average of total anticipated quantity for supply would be 1,650~2,689 MtCO₂e, with annual average of total anticipated demand is 413~490 MtCO₂e, As such the ratio of the total anticipated supply against the total anticipated demand is very high with range of 400~549%, thereby inducing the forecast that substantial quantity of oversupply. This implies that the value of Carbon price within the duration of the reduction (2008-2012) as the responsibilities under the I st Kyoto Protocol. Level of accuracy of analysis must be continually elevated through continuous monitoring as it contains numerous uncertainties including Hot air purchase strategy of counties contained in the Annex 1, early actions of corporations within USA, and possibility of amendment of Kyoto Protocol.

Studies on differential gene expression in Cucumber (Cucumis sativus L.) cultivars by cDNA-AFLP

Seung-In Yi(이승인),In-Ho Song(송인호),Yong-Sham Kwon(권용삼),Kyung-Mi Bae(배경미) 한국원예학회 2004 한국원예학회 학술발표요지 Vol.2004 No.5

종자산업 가치사슬 강화를 위한 종자검정의 역할과 과제

이승인(Seung-in YI) 한국원예학회 2018 한국원예학회 학술발표요지 Vol.2018 No.10

한국의 품종보호 분야 분자표지 연구·활용 현황

이승인(Seung-In Yi ),정진기(Jin-Ki Jung) 한국원예학회 2017 한국원예학회 학술발표요지 Vol.2017 No.10

Isolation and Identification of Secondary Metabolites from the Ovary of Nelumbo nucifera

Seung-Heon Ji(지승헌),Jae-Won Lee(이재원),Seung-Eun Lee(이승은),Young-Seob Lee(이영섭),Geum-Soog Kim(김금숙),Young-Sup Ahn(안영섭),Nam-In Baek(백남인),Yi Lee(이이),Heung-Bin Lim(임흥빈),Dae Young Lee(이대영) 한국생명과학회 2016 생명과학회지 Vol.26 No.10

연꽃의 자방으로부터 80% MeOH로 추출하고, 얻어진 추출물을 n-hexane, ethyl acetate, n-butanol 및 H₂O으로 용매 분획하였다. 이 중 n-hexane 분획물에 대해 silica gel과 octadecyl silica gel column chromatography 및 Prep-HPLC system을 반복 수행하여 5 종의 물질을 분리 하였다. 각 화합물의 화학구조는 NMR, GC/MS및 ESI/MS 등의 분광학적 스펙트럼을 측정하고, 해석하여 1-eicosanol (1), cycloartenol (2), trans-squalene (3), pentadecanoic acid (4) 및 β-sitosterol (5)으로 동정하였다. 이 화합물들은 연꽃 자방추출물에서 처음으로 분리하고 동정하였으며 앞으로 이 화합물들에 대한 다양한 생리적 및 약리적 활성을 검토함으로써 건강기능성 식품 또는 의약품의 소재로서의 충분한 가치가 있다고 여겨진다. The ovary parts of Nelumbo nucifera were extracted in 80% methanol (MeOH), and the concentrated extract was then partitioned using n-hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH), and H₂O, successively. Using an octadecyl silica gel (ODS) column, silica gel (SiO2) column chromatography, and a HPLC purification system, five compounds were isolated from the n-hexane fraction obtained from the extract of N. nucifera ovary. The chemical structures of the metabolites were determined using several spectroscopic methods, including NMR and GC/MS and MS of 1-eicosanol (1), cycloartenol (2), trans-squalene (3), pentadecanoic acid (4), and β-sitosterol (5). This study is a first attempt to isolate and identify secondary metabolites from the ovary of N. nucifera. The results indicated that the extract of N. nucifera ovary has biological effects, such as antibacterial and -tumor activity. Therefore, it could decrease the risk of HIV transmission through breastfeeding.

참비름 ( Amaranthus mangostanus ) 에서 항바이러스성 단백질 ( AAP29 ) 의 분리 및 특성

조강진(Kang Jin Cho),이승인(Seung In Yi),김영태(Yeong Tae Kim),황영수(Young Soo Hwang) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.6

An antiviral protein (AAP29) with ribosome-inactivating activity was purified and characterized from the leaves of the Amaranthus mangostanus. Purification was accomplished through crude extraction, ammonium sulfate precipitation, S-Sepharose chromatography, gel filtration, CM-Sepharose chromatography and Blue sepharose chromatography. This protein was about 29.2 kDa and strongly basic with the PI value between 9.0 and 9.6, indicating that AAP29 is similar to Type 1 RIP. The AAP29 showed high thermostability without activity toss even after 20 min at 50℃. In cell free system using rabbit reticulocyte lysate, AAP29 inhibited protein synthesis with an IC_(50), of 0.18 nM. This protein also reduced mosaic symptoms of cucumber mosaic virus (CMV) on tobacco leaves. The N-terminal amino acid sequences of the AAP29 are ADLTFTVTKDGTSQSYXTLXNXWRXW and shows no suquence similarity with any known RIPS.

SSR 마커를 이용한 복숭아 품종의 유전적 다양성 분석

홍지화(Jee-Hwa Hong),이승인(Seung-In Yi),권용삼9Yong-Sham Kwon),김영(Young Kim),최근진(Keun-Jin Choi) 한국육종학회 2013 한국육종학회지 Vol.45 No.3

The objective of this study was to evaluate genetic diversity in 72 major peach varieties by using SSR markers. A set of 189 SSR primer pairs was screened and 74 primer pairs showed polymorphism in 9 varieties. Twenty primer pairs out of 74 primer pairs showed clear band pattern and repetitive reproducibility. The relationship between 20 markers genotypes and 72 varieties was analyzed. A total of 71 polymorphic amplified fragments were obtained by using 20 SSR markers. Two to nine SSR alleles were detected for each locus with an average of 3.6 alleles per locus. Average polymorphism information content(PIC) was 0.523, ranging from 0.246 to 0.771. A total of 71 marker loci were used to calculate Jaccard’s distance coefficients for cluster analysis using UPGMA. Clustering group was largely divided 2 groups according to absence or presence of pubescence on the fruit surface and genetic distance of cluster ranging from 0.39 to 1.00. Analysis of genetic diversity revealed that these 20 SSR marker sets discriminated a total of 68 varieties except for 4 mutant varieties among 72 varieties. These SSR markers will be utilized as molecular evidence in variety identification of peach.

Simple sequence repeat (SSR) marker를 이용한 벼 품종 식별

권용삼,박은경,박찬웅,배경미,이승인,조일호,Kwon, Yong-Sham,Park, Eun-Kyung,Park, Chan-Ung,Bae, Kyung-Mi,Yi, Seung-In,Cho, Il-Ho 한국생명과학회 2006 생명과학회지 Vol.16 No.6

SSR markers를 이용하여 벼의 품종간 유전적 유연관계 분석과 품종식별 방법에 대한 연구를 수행하여 얻어진 결과를 요약하면 다음과 같다. SSR primer 50개와 벼 보급종 21품종을 PCR 반응시킨 결과 다형성을 뚜렷하게 나타내는 primer는 23개였으며, 각 marker에 의해 발생된 대립유전자의 수는 $2{\sim}9$까지 검출되었고, 평균값은 3.00개로 나타났다. 유전적 다형성 정도를 나타내어 주는 SSR marker의 PIC 값은 최소 0.091에서부터 최대 0.839까지 다양하게 분석되었다. SSR marker를 이용하여 분석된 벼 21품종에 대한 전체 유전적 유사도는 $0.59{\sim}0.92$의 범위에 속하였고 유사도 지수 0.65를 기준으로 할 때 4개의 그룹으로 구분되었다. SSR marker중에서 RM206, RM225, RM418, RM478은 marker genotype에 의해 21 품종에 대해 각각 고유한 밴드 특성을 나타내어 품종판별이 가능한 것으로 나타났다. 금후 이 연구결과는 벼 보급종의 품종식별을 위해 효과적으로 이용될 수 있는 것으로 나타났다. The objective of this study was carried out to evaluate the suitability of simple sequence repeat (SSR) markers for genetic diversity assessment and identification of rice varieties. The 23 primers selected from 50 SSR primers showed polymorphisms in the 21 rice varieties. The 2 to 9 SSR alleles were detected for each locus with an average of 3.00 alleles per locus. The polymorphism information content (PIC) ranged form 0.091 to 0.839. Based on band patterns, UPGMA cluster analysis was conducted. These varieties were separate into 4 groups corresponding to rice ecotype and pedigree information and genetic distance of cluster ranging from 0.59 to 0.92. The 4 SSR primer sets (RM206, RM225, RM418, RM478) selected form 23 polymorphic primers were differentiated all the rice variety from each other by markers genotypes. These markers may be used wide range of practical application in variety identification of rice.