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홍성택,손석용,노창우,이경희,정재현,박재성 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.4
공시된 들깨 지방재래종 90개 품종의 단백질함량, 지방 및 지방산을 분석한 결과 다음과 같다. 1. 숙기별로는 중ㆍ만생종의 평균단백질 함량은 22.4%로 비교적 낮았고, 품종분포가 대부분 평균치에 가까웠으며, 조생종은 평균단백질 함량이 24.1%로 비교적 높았다. 2. 평균단백질함량은 소립종(천립중3.0~4.0g이하)이 23.0%로, 중립종(천립중 4.0~5.0g)이나 대립종(천립중 5.0~6.0g)의 22.1%보다 높은 경향이었다. 3. 기름함량은 조생종이 42.6%로 가장 적었고, 중생종이 44.5%, 만생종이 45.5%로 기름함량이 많았으며, 립중별로는 소립종이 44.2%, 중립종이 43.1%, 대립종이 41.5% 대립종일수록 낮은 경향이었다. 4. 지방산조성은 숙기가 늦어짐에 따라 Oleic acid와 Linolenic acid는 다소 많았고, Linoleic acid는 적어지는 경향이었다. Linoleic acid는 극대립종에서 15.5%로 가장 높았고 립중이 가벼울수록 높아지는 경향이었다. 5. 기름함량과 지방산 비율간의 상관 정도를 보면 기름함량과 Palmitic acid, Linoleic acid는 負의 상관이나, Stearic acid, Linolenic acid는 正의 상관을 보였다. Stearic acid는 Oleic acid와 유의적인 정의 상관을 , Linoleic acid와는 부의 상관을 보였다. Oleic acid는 Linoleic acid 및 Linolenic acid와 고도로 유의한 부의 상관을, Linoleic acid는 Linolenic acid와, 포화지방산은 불포화지방산과 유의한 부의 상관을 보였다. Korean local collected strains or varieties of perilla analyzed on their protein and oil content and fatty acids composition in the seed. The strains analyzed were 90 of which grown in the experimental field of Chungbuk Agricultural Research and Extension Services at Cheongwon and Umseong from 2001 to 2002. The seed protein contents of 90 perilla varieties ranged from 19.1% to 29.0% and the average content was 22.8%. There differed by the seed coat color showing 1.9% higher content in light gray than that of dark brown, which may suggest that the seed coat color could be used as a marker gene for breeding high protein varieties. Oil content of medium and late varieties in maturity showed higher as compared to early varieties, and appeared differences by seed coat color, and small seed varieties showed higher seed oil content than in large seed varieties by seed size. Linoleic and linolenic acid content of which major fatty acids in perilla seed were 74.4% in average of tested varieties. Fatty acid composition of perilla varieties were not significantly different by maturity, seed coat color and 1,000 seed weight. Oil content showed high positive correlation, with stearic and linolenic acids and negative correlation with linoleic acid.
활동기 척추동물의 십이지장 배상세포내 점액질에 관한 조직화학적 연구
박래백,한현주,임영빈,김효성,박경수,노영복 조선대학교 기초과학연구소 1990 自然科學硏究 Vol.13 No.1
본 실험은 다람쥐(Tamias sibiricus asiaticus Gmeli), 살모사(Agkistrodon halys Pallas), 황소개구리(Rana catesbeiana Shaw), 뱀장어(Anguilla japonica Temminck et Schlegel)등의 소장 배상세포내 점액질에 대한 성상을 밝히기 위하여 PAS reaction, Alcian blue pH2.5 및 Alcian blue pH0.4, 그리고 ABpH2.5-PAS reaction 복합염색등을 시행한 결과 다음과 같은 결론을 얻었다. 1. 사용한 각 동물에 있어서 소장 배상세포는 중성점액질, 약산성 및 강산성 황화점액질을 포함하고 있었다. 2. 중성점액질이 각 동물에 있어서 가장 안정된 분포를 보였으며 약산성 황화점액질도 비교적 고르게 분포되어 있었다. 3. 강산성 황화점액질은 다람쥐, 살모사 및 황소개구리에서는 소량 관찰되어 불안정한 분포를 보였으며 뱀장어에서는 관찰되지 않았다. 4. 각 실험동물의 십이지장 배상세포내에 포함된 점액질의 성상은 동물에 따라서 그 양과 염색성에 다소의 차이를 인정할 수 있다. In general, the goblet cells in the small intestines of experimental animals contained neutral mucosubstances, weakly and strongly acidic sulfated mucosubstances. Neutral mucosubstances show most stable distribution in the experimental animals. Mucosubstances in the small intestines of eels were composed of only neutral mucosubstances and weakly acidic sulfated mucosubstances. The contents and stain properties of mucostances produced in the intestinal goblet cells vary a little in portions within a species and in the same cell types of different species. This studies were performed in order to study composition of the mucosubstance in the goblet cells of small intestines of the various vertebrates. The specimens used in this study were obtained from the small intestines of 4 species of vertebrates, squirrels (Tamias sibiricus asiaticus Gmelin), vipers (Agkistrodon halys Palls), frogs (Rana catesbeiana Shaw), eels (Anguilla japonica Temminck et Scchlegel). All specimens were fixed in 10% neutral buffered formalin, embedded in paraffin wax, and sectioned at a thickness of 4㎛. They were stained with hematoxylin-eosin for general histological observation and with Periodic Acid-Schiff reaction, alcian blue pH2.5 and 0.4 and alcian blue pH2.5-Periodic Acid-Schiff reaction for detection of the mucosubstances. The mucous compositions in the goblet cells of the small intestines of various vertebrates examined by their staining reaction were neutral mucosubstances, weakly and strongly acidic sulfated mucosubstances.
Seong, Rho Hyun 이화여자대학교 세포신호전달연구센터 2002 고사리 세포신호전달 심포지움 Vol. No.4
It has been previously reported that there is a significant correlation between the expression level of SRG3 and GC sensitivity of developing thymocytes. The expression level of SRG3 plays a role as a key determinant of SRG3-GR complex formation and thus the sensitivity to GC-induced apoptosis of T cells by regulating GR-mediated transcription. Furthermore, SRG3 expression decreased to one third after positive selection. During maturation in the thymus, signaling through TCR/CD3 complex is one of key determinants that affects the cell fate decision of developing thymocytes. Therefore, in this study, the effect of TCR/CD3 signaling on the expression of the SRG3 gene using murine immature thymoma cell lines, 16610D9 and S49.1 cells, was examined. TCR/CD3 engagement resulted in a dramatic decrease in protein expression as well as the promoter activity of the SRG3 gene via Ras activation. Specifically, TCR/CD3 downregulation of the SRG3 gene was mediated by Ras/MEK/ERK and/or PI3K pathway. In addition, the 130bp SRG3 minimal promoter region was shown to be maximally suppressed upon T cell stimulation. Moreover, E47/HEB heterodimer bound to the E-box element in the SRG3 minimal promoter, which was inhibited by Id3 inhibitor of E proteins. Finally, introduction of mutations into the proximal E-box element in the SRG3 promoter completely abrogated the TCR/Ras responsiveness of the SRG3 promoter. These results strongly imply that the expression of the SRG3 gene is downregulated by signaling through TCR/CD3 complexes, rendering CD4^(+)CD8^(+) DP thymocytes GC resistance. During positive selection, in particular, DP thymocytes that express low level of SRG3 in response to TCR/CD3 signaling may become resistant to GCs and differentiate into functional T cells. However, in the absence of these signaling, DP thymocytes may still express high level of SRG3 and thus be eliminated by GC-induced apoptosis.
Seong, Rho Hyun 이화여자대학교 세포신호전달연구센터 2006 고사리 세포신호전달 심포지움 Vol. No.8
The process of thymocyte development requires an exquisite regulation of the expression of many genes by transcriptionfactors and chromatin remodeling activities. Even though the SWI/SNF chromatin remodeling complex has been thought to play important roles during thymocyte development, its known function is very limited and the regulation of the remodeling activity is not known. Here we show that the SWI/SNF chromatin remodeling activity is finely regulated during thymocyte maturation process, especially during thymocyte selections. SRG3, a mouse homolog of yeast SWI3 and human BAF155, controls the stability of the other core components of the SWI/SNF complex at the post-transcriptional level. The stability of BRG1, an ATPase subunit of the complex, and SNF5/INI1/BAF47, another core component of the SWI/SNF complex, is dependent on its interaction with the SRG3 protein. The change in the SRG3 expression resulted in the change in the protein level of the chromatin remodeling complex and also its activity in thymocytes. SRG3 is a direct target of T-cell receptor signaling during the positive selection. Constitutive expression of SRG3 in developing thymocytes resulted in the increased chromatin remodeling activity in DP thymocytes and disrupted normal processes of both positive and negative selections. Our results state, for the first time, that the chromatin remodeling activity needs to be finely controlled for proper thymocyte selection and maturation processes.
Seong, Rho Hyun 가톨릭 의과학연구원 2001 가톨릭 의과학연구원 국제학술대회 Vol.5 No.-
Immature, double positive thymocytes are sensitive to glucocorticoid-induced apoptosis, while mature single positive T cells are relatively resistant. These double positive thymocytes seem to acquire resistance to glucocorticoids during differentiation into mature CD+4+ and CD^8+ single positive thymocytes. However, detailed knowledge concerning what determines the sensitivity of thymocytes to glucocorticoids and how glucocorticoid-sensitivity is regulated in thymocytes during development is lacking. We found that the expression level of SRG3 protein, which is a mouse homolog of yeast SWI3 and a component of SWI/SNF complex, determines the glucocorticoid-sensitivity of Tcells in mice.
PSCAD/EMTDC를 이용한 ±35kV급 MVDC 하이브리드 배전망의 사고해석에 관한 연구
강성현(Seong-Hyun Kang),이후동(Hu-Dong Lee),이성두(Seong-Doo Lee),노대석(Dae-Seok Rho) 대한전기학회 2021 전기학회논문지 Vol.70 No.8
Based on ±35kV-scale MVDC hybrid distribution system to demonstrate MVDC distribution technology, this paper presents scenarios of contingency fault analysis and modeling for impact evaluation on MVDC hybrid distribution system in order to examine the operation characteristics of MVDC facilities and customers. And also, this paper proposes a modeling of the ±35kV-scale MVDC hybrid distribution system using PSCAD/EMTDC, which is composed of distribution substation, MVDC distribution feeder, converter station and PV system. From the simulation results of fault analysis in MVDC distribution system based on the modeling for impact evaluation on distribution system with the fault scenarios, it is confirmed that tremendous fault current can be momentarily occurred due to discharging of DC link capacitor in converter station regardless of fault type. Furthermore, it is found that the faults in MVDC feeder can cause the phenomenon of under-voltage at customers in other feeder of the main transformer. In addition, it is clear that the malfunction of recloser in other feeder can be prevented due to ungrounded system of MVDC system, and also the voltages of AC 22.9kV bus and MVDC feeder are maintained in a stable manner even if ground fault is occurred in AC 22.9kV feeder.