Endoplasmic Reticulum Mediated Necrosis-Like Apoptosis of Hela Cells Induced by Ca(2+) Oscillation

Qing Liu Hu,Jun Lei Chang,Li Tao Tao,Guo Liang Yan,Ming Chao Xie,Zhao Wang 한국생활환경학회 2005 BMB Reports Vol.38 No.6

SMYD3-associated pathway is involved in the anti-tumor effects of sulforaphane on gastric carcinoma cells

Qing-Qing Dong,Qiu-Tong Wang,Lei Wang,Ya-Xin Jiang,Mei-Ling Liu,Hai-Jie Hu,Yong Liu,Hao Zhou,Hong-Peng He,Tong-Cun Zhang,Xuegang Luo 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.4

Sulforaphane (SFN), a natural compound derived from cruciferous vegetables, has been proved to possess potent anti-cancer activity. SMYD3 is a histone methyltransferase which is closely related to the proliferation and migration of cancer cells. This study showed that SFN could dose-dependently induce cell cycle arrest, stimulate apoptosis, and inhibit proliferation and migration of gastric carcinoma cells. Accompanied with these anticancer effects, SMYD3 and its downstream genes, myosin regulatory light chain 9, and cysteine-rich angiogenic inducer 61, was downregulated by SFN. Furthermore, overexpression of SMYD3 via transfection could abolish the effects of SFN, suggesting that SMYD3 might be an important mediator of SFN. To the best of our knowledge, this is the first report describing the role of SMYD3 in the anti-cancer of SFN. These findings might throw light on the development of novel anti-cancer drugs and functional food using SFN-rich cruciferous vegetables.

Three new diphenylpropanes from Celastrus hindsii

Xian Qing Hu,Wei Han,Zhu Zhen Han,Qing Xin Liu,Xi-Ke Xu,Peng Fu,Hui-Liang Li 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.11

Three new diphenylpropanes, HindsiipropaneA–C (1–3), together with one known arylpropyl quinoneGriffithane D (4), were isolated from Celastrus hindsii. Their structures were established by 1D and 2D nuclearmagnetic resonance spectroscopic analysis, and massspectroscopy. Compound 4 was firstly obtained in thisgenus. All the isolated compounds were evaluated in vitrofor cytotoxicity against four human tumor cell lines (A549,HCT116, MDA-MB-231, BEL7404) by the MTT assay.

Single Nucleotide Polymorphisms of the GnRHR Gene Associated with Reproductive Traits of Japanese Flounder (Paralichthys olivaceus)

He, Feng,Wen, Hai-Shen,Li, Ji-Fang,Yu, Da-Hui,Ma, Rui-Qin,Shi, Dan,Mu, Wei-Jie,Zhang, Yuan-Qing,Hu, Jian,Liu, Miao,Han, Wei-Guo,Zhang, Jia-Nan,Wang, Qing-Qing,Yuan, Yu-Ren,Liu, Qun Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.4

Gonadotropin-releasing hormone receptor (GnRHR) gene is expressed at the anterior pituitary gland and plays a key role in gonad development. This study aimed to investigate molecular genetic characteristics of the GnRHR gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of GnRHR gene on sex steroid level in Japanese flounder (Paralichthys olivaceus). We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the GnRHR gene in 75 individuals. We identified three SNPs in the GnRHR gene: P1 locus (C759A and C830T) in the coding region of exon2 which were both linked together and P2 locus (G984T) in the coding region of exon3, which added a new transcript factor (ADR1) and a new methylation site (CG). Only C830T of P1 leads to amino acid changes Thr266Ile. Statistical analysis showed that P1 was significantly associated with $17{\beta}$-estradiol ($E_2$) level (p<0.01) and gonadosomatic index (GSI) (p<0.05). Individuals with genotype BB of P1 had significantly higher serum $E_2$ levels (p<0.01) and GSI (p<0.05) than those of genotype AA or AB. Another SNP, P2, synonymous mutation, was significantly associated with GSI (p<0.05). Individuals with genotype AB of P2 had significantly higher GSI (p<0.05) than that of genotype AA. In addition, there was a significant association between one diplotype based on three SNPs and reproductive traits. The genetic effects for both serum $E_2$ level and GSI of diplotype D4 were super diplotypes (p<0.05). These results suggest that the SNPs in Japanese Flounder GnRHR are associated with $E_2$ level and GSI.

Population dynamics of Frankliniella occidentalis Pergrande and its predator Orius similis Zheng on common crops and surrounding plants

Hu Changxiong,Li Yiru,Chen Guohua,Duan Pan,Wu Daohui,Liu Qing,Yin Honghui,Xu Tianyang,Zhang Xiaoming 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Frankliniella occidentalis Pergrande is important invasive pests in China, causing damage to agricultural pro duction, and Orius similis Zheng is the dominant predator species of F. occidentalis. A two-year survey was conducted to determine the population density of F. occidentalis and O. similis, on chili (Capsicum annuum L.) and maize (Zea mays L.) crops and surrounding weed species, which included white clover (Trifolium repens L.), St. John’s wort (Hypericum beanii N. Robson), alfalfa (Medicago sativa L.) and beggarticks (Bidens pilosa L.) in Kunming, southern China. The activity of F. occidentalis on these 6 host plant species was determined using the quartile method. F. occidentalis mainly damaged plants during their flowering stage. The main activity period of F. occidentalis occurred earlier on H. beanii and T. repens than on C. annuum. The peak activity of F. occidentalis occurred in the middle of May (on T. repens). During the whole activity period, the highest thrips densities were recorded on H. beanii among all of the sampled host plant species, followed by C. annuum. The lowest density was recorded on B. pilosa. Dynamics of immature F. occidentalis were more irregular than that of adults. The highest density of O. similis was recorded on Z. mays. It was 2.27–26.43-fold (2017) and 2.01–19.09-fold (2018) higher than that on other host plant species. This study showed that F. occidentalis could migrate between C. annuum and surrounding weeds. The weeds were the main source of thrips on C. annuum. The results indicated that Z. mays can be planted around C. annuum fields as a potential banker plant, to attract O. similis to control F. occidentalis on C. annuum, T. repens and B. pilosa. The flowering period of plants and surrounding plant species has a great effect to the population activities of F. occidentalis and predator O. similis on crops.

Protective Effect of Astragalus polysaccharides on Liver Injury Induced by Several Different Chemotherapeutics in Mice

Liu, Wen,Gao, Fang-Fang,Li, Qun,Lv, Jia-Wei,Wang, Ying,Hu, Peng-Chao,Xiang, Qing-Ming,Wei, Lei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Side effects are an unavoidable consequence of chemotherapy drugs, during which liver injury often takes place. The current study was designed to investigate the protective effect of Astragalus polysaccharides (APS) against the hepatotoxicity induced by frequently-used chemical therapy agents, cyclophosphamide (CTX), docetaxel (DTX) and epirubicin (EPI)) in mice. Mice were divided into five groups, controls, low or high dose groups ($DTX_L$, $CTX_L$, $EPI_L$ or $DTX_H$, $CTX_H$, $EPI_H$), and low or high dose chemotherapeutics+APS groups ($DTX_L$+APS, $CTX_L$+APS, $EPI_L$+APS or $DTX_H$+APS, $CTX_H$+APS, $EPI_H$+APS). Controls were treated with equivalent normal saline for 28 days every other day; low or high dose group were intraperitoneal (i.p) injected with low or high doses of CTX, DTX and EPI for 28 days every other day; low or high dose chemotherapeutics+APS group were separately intraperitoneal (i.p) injected with chemotherapeutics for 28 days every other day and i.p with APS (100 mg/kg) for 7 days continually from the 22th to the 28th days. The body weight, serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), histopathological features, and ultrastructure morphological change of liver tissues, protein expression level of caspase-3 were estimated at different time points. With high dose treatment of DTX, CTX and EPI, weight gain was inhibited and serum levels of ALT and AST were significantly increased. Sections of liver tissue showed massive hepatotoxicity in $CTX_H$ group compared to the control group, including hepatic lobule disorder, granular and vacuolar degeneration and necrosis in hepatic cells. These changes were confirmed at ultrastructural level, including obvious pyknosis, heterochromatin aggregation, nuclear membrane resolution, and chondrosome crystal decrease. Western blotting revealed that the protein levels of caspase-3 increased in $CTX_H$ group. The low dose groups exhibited trivial hepatotoxicity. More interestingly, after 100 mg/kg APS, liver injury was redecued not only regarding serum transaminase activities (low or high dose chemotherapeutics+APS group), but also from pathological and ultrastructural changes and the protein levels of caspase-3 ($CTX_H$+APS group). In conclusion, DTX, CTX and EPI induce liver damage in a dose dependent manner, whereas APS exerted protective effects.

Experimental Investigation into In-plane Stability of Concrete-Filled Steel Tubular Parabolic Arches Under Five-Point Concentrated Loads

Qing Hu,Changyong Liu,Changchun Yuan,Yuyin Wang,Sumei Zhang 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.6

This paper deals with experimental investigations into in-plane stability of fi xed concrete-fi lled steel tubular (CFST) parabolic arches. Three CFST arches with the same span but diff erent rise-to-span ratios were tested under fi ve-point symmetrical concentrated loads over the full span. All applied loads were controlled in synchronization. The test results show that the test arches buckled in an antisymmetric failure mode and the section positions with the maximum deformation were slightly diff erent for three test arches. It is found that the bearing capacity of CFST arches decreased signifi cantly with the decline of rise-to-span ratios, and the outer steel tube provided signifi cant confi nement eff ects on the core concrete after the load reaches 80% of the load-carrying capacity. Moreover, comparisons between the test and fi nite element results indicate that the existing beam-element modeling method can predict the in-plane stability performance of CFST arches very well.

Optically tunable Seebeck effect from intramolecular proton-transfer materials in organic vertical thin-film thermoelectric device

Hu, Dehua,Liu, Qing,Tisdale, Jeremy,Nam, Haerim,Park, Soo Young,Wang, Hsin,Urbas, Augustine,Hu, Bin Elsevier 2015 ORGANIC ELECTRONICS Vol.26 No.-

<P><B>Abstract</B></P> <P>This paper reports Seebeck effects from optically-induced intramolecular proton-transfer HPI-Cbz molecules based on vertical electrode/organic film/electrode thin-film devices. We observed large Seebeck coefficients of 428μV/K and 390μV/K from HPI-Cbz based thin-film devices at 60°C when proton-transfer was induced by the photoexcitation of a 325nm laser with an intensity of 12mW/cm<SUP>2</SUP> and 6mW/cm<SUP>2</SUP> respectively. Under dark condition without proton transfer occurring, the Seebeck coefficient was measured to be 342μV/K at 60°C. The Seebeck coefficient enhancement by the induced intramolecular charge transfer can be attributed to the enhanced polarization difference between high- and low-temperature surface due to the stronger electron–phonon coupling followed with the proton-transfer in HPI-Cbz under photoexcitation, and the strength of electron–phonon coupling is proportional to the photoexcitation intensity. The enhanced temperature-dependent electrical polarization between the high and low-temperature surfaces acts as an additional driving force to diffuse the majority charge carriers for the development of a large Seebeck effect. Therefore, using intramolecular proton-transfer presents an effective approach of enhancing Seebeck effect in organic materials.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Photoinduced proton-transfer states lead to enhancement on Seebeck coefficients. </LI> <LI> Photoinduced proton-transfer states lead to enhancement on electrical conductivity. </LI> <LI> Temperature-dependent polarization acts as a new driving force for Seebeck effect. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Oxalate Decarboxylase from Agrobacterium tumefaciens C58 is Translocated by a Twin Arginine Translocation System

( Yu Hu Shen ),( Rui Juan Liu ),( Hai Qing Wang ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.7

Use of In Vivo-Induced Antigen Technology to Identify In Vivo-Expressed Genes of Campylobacter jejuni During Human Infection

( Yuan Qing Hu ),( Jin Lin Huang ),( Qiu Chun Li ),( Yu Wei Shang ),( Fang Zhe Ren ),( Yang Jiao ),( Zhi Cheng Liu ),( Zhi Ming Pan ),( Xin An Jiao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.3

Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in vivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during human infection by C. jejuni. An inducible expression library of genomic proteins was constructed from sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled human sera obtained from clinical patients. We successfully identified 24 unique genes expressed in vivo. These genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, and other processes. We selected six genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results showed that the selected six genes were significantly upregulated in vivo but not in vitro. In short, these identified in vivo-induced genes may contribute to human infection of C. jejuni, some of which may be meaningful vaccine candidate antigens or diagnosis serologic markers for campylobacteriosis. IVIAT may present a significant and efficient method for understanding the pathogenicity mechanism of Campylobacter and for finding targets for its prevention and control.