Antifungal and carboxylesterase-producing bacteria applied into corn silage still affected the fermented total mixed ration

Paradhipta Dimas Hand Vidya,Seo Myeong Ji,Jeong Seung Min,주영호,Lee Seong Shin,Seong Pil-Nam,이혁준,김삼철 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.5

Objective: This study investigated the effects of corn silage as a source of microbial inoculant containing antifungal and carboxylesterase-producing bacteria on fermentation, aerobic stability, and nutrient digestibility of fermented total mixed ration (FTMR) with different energy levels. Methods: Corn silage was used as a bacterial source by ensiling for 72 d with an inoculant mixture of Lactobacillus brevis 5M2 and L. buchneri 6M1 at a 1:1 ratio. The corn silage without or with inoculant (CON vs MIX) was mixed with the other ingredients to formulate for low and high energy diets (LOW vs HIGH) for Hanwoo steers. All diets were ensiled into 20 L mini silo (5 kg) for 40 d in quadruplicate. Results: The MIX diets had lower (p<0.05) acid detergent fiber with higher (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber compared to the CON diets. In terms of fermentation characteristics, the MIX diets had higher (p<0.05) acetate than the CON diets. The MIX diets had extended (p<0.05) lactic acid bacteria growth at 4 to 7 d of aerobic exposure and showed lower (p<0.05) yeast growth at 7 d of aerobic exposure than the CON diets. In terms of rumen fermentation, the MIX diets had higher (p<0.05) total fermentable fraction and total volatile fatty acid, with lower (p<0.05) pH than those of CON diets. The interaction (p = 0.036) between inoculant and diet level was only found in the immediately fermentable fraction, which inoculant was only effective on LOW diets. Conclusion: Application of corn silage with inoculant on FTMR presented an antifungal effect by inhibiting yeast at aerobic exposure and a carboxylesterase effect by improving nutrient digestibility. It also indicated that fermented feedstuffs could be used as microbial source for FTMR. Generally, the interaction between inoculant and diet level had less effect on this FTMR study.

Effects of wild or mutated inoculants on rye silage and its rumen fermentation indices

Paradhipta, Dimas Hand Vidya,Joo, Young Ho,Lee, Hyuk Jun,Lee, Seong Shin,Kwak, Youn Sig,Han, Ouk Kyu,Kim, Dong Hyeon,Kim, Sam Churl Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.6

Objective: This study was conducted to confirm the effects of new inoculants producing-antifungal or esterase substances on rye silage and its rumen fermentation indices by comparing wild with mutated types. Methods: Rye harvested at dough stage was ensiled into 3 L mini bucket silo (1 kg) for 90 d in triplicate following: distilled water at 20 μL/g (CON); Lactobacillus brevis 100D8 (AT) and its inactivation of antifungal genes (AT-m) at 1.2×10⁵ cfu/g, respectively; and Leuconostoc holzapfelii 5H4 (FD) and its inactivation of esterase genes (FD-est) at 1.0×10⁵ cfu/g, respectively. After silo opened, silage was sub-sampled for the analysis of ensiling quality and its rumen fermentation indices. Results: Among the wild type inoculants (CON vs AT vs FD), FD inoculant had higher (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber, the total degradable fraction, and total volatile fatty acid in rumen, while AT inoculant had higher (p<0.05) lactate, acetate, and lactic acid bacteria in silage. Silage pH and the potentially degradable fraction in rumen increased (p<0.05) by inactivation of antifungal activity (AT vs AT-m), but lactate, acetate, and lactic acid bacteria of silage decreased (p<0.05). In silage, acetate increased (p<0.05) by inactivation of esterase activity (FD vs FD-est) with decreases (p<0.05) of pH, ammonia-N, lactate, and yeast. Moreover, inactivation of esterase activity clearly decreased (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber, the total degradable fraction, and total volatile fatty acid in the rumen. Conclusion: This study concluded that FD inoculant confirmed esterase activity on rye silage harvested at dough stage, while AT inoculant could not be confirmed with antifungal activity due to the absence of mold in all silages.

6. Effects of New Antifungal and Esterase Inoculant on Fermentation Characteristics and Rumen Digestibility of Corn Silage

D. H. V. Paradhipta,Y. H. Joo,H. J. Lee,S. S. Lee,B. S. Kang,J. W. Kim,H. G. Min,S. C. Kim 한국초지조사료학회 2019 한국초지조사료학회 학술대회논문집 Vol.2019 No.06

Effects of Potato By-products Containing Glycoalkaloid on Rumen Fermentation Characteristics

Dimas Hand Vidya Paradhipta,이혁준,주영호,이성신,강동훈,정기용,김삼철 경상국립대학교 농업생명과학연구원 2020 농업생명과학연구 Vol.54 No.4

The present study was conducted to investigate the effects of feed types (powder vs. pellet) from potato by-product containing glycoalkaloid on rumen fermentation with different rumen pH (low vs. high) using in vitro technique. Rumen fluid was collected from two cannulated Hanwoo heifers before morning feeding (high rumen pH at 6.71) and 3 h after feeding (low rumen pH at 6.20). A 0.5 gram of powder or pellet from potato by-product was incubated in the rumen buffer for 48 h at 39℃. Four replications for each treatment were used along with two blanks. Pellet had higher (p<0.05) the immediately degradable fraction, the fractional degradation rate, and the lag phase than those of powder. However, feed type had no effects on the potentially degradable fraction and the total degradable fraction. In fermentation characteristics, powder had higher (p<0.05) concentrations of ammonia-N, total volatile fatty acid (VFA), and acetate than those of pellet. In addition, a high rumen pH had higher (p<0.05) concentrations of ammonia-N and acetate, but lower (p<0.05) total VFA concentration than those of a low rumen pH. The interaction effects between feed type and rumen pH were found on the total degradable fraction and concentrations of ammonia-N, total VFA, and acetate. The present study concluded that generally feed type had no effects on rumen degradation kinetics, but potato by-product in the powder form is recommended due to produce higher fermentation characteristics than in the pellet form.

혼합 생균제 급여가 거세한우의 성장, 혈액성상 및 육질에 미치는 영향

( Dimas H. V. Paradhipta ),전충환 ( Chung-hwan Jeon ),최인학 ( In-hag Choi ),이혁준 ( Hyuk-jun Lee ),주영호 ( Young-ho Joo ),이성신 ( Sung-shin Lee ),김동현 ( Dong-hyeon Kim ),김삼철 ( Sam-churl Kim ) 한국환경과학회 2017 한국환경과학회지 Vol.26 No.8

In this study, the effect of probiotic supplementation on growth performance, blood metabolites, and meat quality of Hanwoo steer was investigated. A total of 32 Hanwoo steers (15-17 months, average body weight 462±37.9 kg) were randomly allotted to 4 dietary treatments (0, 0.5, 1.0, and 1.5% mixed probiotics), with four Hanwoo steers per pen (two replicates per treatments), and reared for 12 months. There were no differences among treatments in growth performance of Hanwoo steer (P>0.05); however, feed intake decreased linearly with increasing levels of mixed probiotics. Growth hormone and Blood Urea Nitrogen (BUN) levels responded linearly with increasing levels of dietary mixed probiotics (P<0.05), but not insulin and blood glucose did not. In particular, total cholesterol was significantly lower for the 1% mixed probiotic treatment in comparison with that of the other treatments (P<0.05). The pH, Thiobarbituric Acid Reactive Substances (TBARS), cooking loss, and meat color were influenced by increasing levels of mixed probiotics (P<0.05), but the carcass characteristics and shear force were not. Regarding sensory evaluation, the addition of mixed probiotics resulted in significant difference in meat color, tenderness, aroma, off-flavor, juiciness, and marbling score, but not in overall acceptability. In addition, fatty acid profiles indicated no differences between control and mixed probiotic treatments. In conclusion, mixed probiotic treatment at 1% levels can enhance consumer preferences possibly by reducing cholesterol and TBARS.

40. Evaluation of Fermentation Quality of Corn Silage and Isolation of Antifungal Epiphytic Bacteria

D. H. V. Paradhipta,S. S. Lee, B. Kang,Y. H. Joo,H. Y. Lee,S. Hyeon,J. Kim,S. C. Kim 한국초지조사료학회 2018 한국초지조사료학회 학술대회논문집 Vol.2018 No.09

3. Effects of New Inoculants on Fermentation Quality of Whole Crop Rice Silage with Different Moisture Contents

D. H. V. Paradhipta,Y. H. Joo,H. J. Lee,S. S. Lee,J. S. Choi,H. T. Noh,K. B. Ji,S. C. Kim 한국초지조사료학회 2020 한국초지조사료학회 학술대회논문집 Vol.2020 No.09

Application of lactic acid bacteria producing antifungal substance and carboxylesterase on whole crop rice silage with different dry matter

Lee Seong Shin,PARADHIPTA DIMAS HAND VIDYA,이혁준,주영호,Noh Hyeon Tak,최정석,Ji Keum Bae,김삼철 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.6

Objective: This study was conducted to investigate effects of antifungal substance and carboxylesterase-producing inoculant on fermentation indices and rumen degradation kinetics of whole crop rice (WCR) silage ensiled at different dry matter (DM) contents. Methods: Dual-purpose inoculants, Lactobacillus brevis 5M2 and Lactobacillus buchneri 6M1, confirmed both activities of antifungal and carboxylesterase in the previous study. The WCR at mature stage was chopped, and then wilted to obtain three different DM contents consisting of 35.4%, 43.6%, and 51.5%. All WCR forages were applied distilled water (CON) or mixed inoculants with 1:1 ratio at 1×105 colony forming unit/g (INO), and ensiled into 20 L mini silo (5 kg) in quadruplicates for 108 d. Results: The INO silages had lower lactate (p<0.001) and butyrate (p = 0.022) with higher acetate (p<0.001) and propionate (p<0.001) than those of CON silages. Ammonia-N (p<0.001), lactate (tendency; p = 0.068), acetate (p = 0.030), and butyrate (p<0.001) concentrations of INO silages decreased linearly with increasing DM content of WCR forage. The INO silages presented higher lactic acid bacteria (p<0.001) with lower molds (p< 0.001) than those of CON silages. Yeasts (p = 0.042) and molds (p = 0.046) of WCR silages decreased linearly with increasing DM content of WCR forage. In the rumen, INO silages had higher the total degradable fraction (p<0.001), total volatile fatty acid (tendency; p = 0.097), and acetate (p = 0.007), but lower the fractional degradation rate (p = 0.011) and propionate (p<0.001) than those of CON silage. The total degradable fraction (p<0.001), total volatile fatty acid (p = 0.001), iso-butyrate (p = 0.036), and valerate (p = 0.008) decreased linearly with increasing DM content of WCR forage, while the lag phase (p<0.001) was increased linearly. Conclusion: This study concluded that application of dual-purpose inoculants on WCR silage confirmed antifungal and carboxylesterase activities by inhibiting mold and improving rumen digestibility, while increase of wilting times decreased organic acids production and rumen digestibility. Objective: This study was conducted to investigate effects of antifungal substance and carboxylesterase-producing inoculant on fermentation indices and rumen degradation kinetics of whole crop rice (WCR) silage ensiled at different dry matter (DM) contents.Methods: Dual-purpose inoculants, Lactobacillus brevis 5M2 and <i>Lactobacillus buchneri</i> 6M1, confirmed both activities of antifungal and carboxylesterase in the previous study. The WCR at mature stage was chopped, and then wilted to obtain three different DM contents consisting of 35.4%, 43.6%, and 51.5%. All WCR forages were applied distilled water (CON) or mixed inoculants with 1:1 ratio at 1×10⁵ colony forming unit/g (INO), and ensiled into 20 L mini silo (5 kg) in quadruplicates for 108 d.Results: The INO silages had lower lactate (p<0.001) and butyrate (p = 0.022) with higher acetate (p<0.001) and propionate (p<0.001) than those of CON silages. Ammonia-N (p<0.001), lactate (tendency; p = 0.068), acetate (p = 0.030), and butyrate (p<0.001) concentrations of INO silages decreased linearly with increasing DM content of WCR forage. The INO silages presented higher lactic acid bacteria (p<0.001) with lower molds (p< 0.001) than those of CON silages. Yeasts (p = 0.042) and molds (p = 0.046) of WCR silages decreased linearly with increasing DM content of WCR forage. In the rumen, INO silages had higher the total degradable fraction (p<0.001), total volatile fatty acid (tendency; p = 0.097), and acetate (p = 0.007), but lower the fractional degradation rate (p = 0.011) and propionate (p<0.001) than those of CON silage. The total degradable fraction (p<0.001), total volatile fatty acid (p = 0.001), iso-butyrate (p = 0.036), and valerate (p = 0.008) decreased linearly with increasing DM content of WCR forage, while the lag phase (p<0.001) was increased linearly.Conclusion: This study concluded that application of dual-purpose inoculants on WCR silage confirmed antifungal and carboxylesterase activities by inhibiting mold and improving rumen digestibility, while increase of wilting times decreased organic acids production and rumen digestibility.

Effects of Selected Inoculants on Chemical Compositions and Fermentation Indices of Rye Silage Harvested at Dough Stage

Lee, Seong Shin,Paradhipta, Dimas H.V.,Joo, Young Ho,Lee, Hyuk Jun,Kwak, Youn Sig,Han, Ouk Kyu,Kim, Sam Churl The Korean Society of Grassland and Forage Science 2018 한국초지조사료학회지 Vol.38 No.2

This study was carried out to estimate the effect of selected inoculants on chemical compositions and fermentation characteristics of rye silage. Rye was harvested at dough stage and divided into 5 treatments, following: No additives (CON); L. plantarum R48-27 (LP27); L. buchneri R4-26 (LB26); Mixture of LP27 and LB26 at 1:1 ratio (MIX); and L. buchneri (LB). The rye forage was ensiled into 10 L bucket silo for 100 days. The contents of NDF and ADF were lowest (P<0.05) in LB26. The pH in LB26, MIX, and LB were lower (P<0.05) than CON and LP27. Lactate content in LB was higher (P<0.05) than the others, while acetate content in LB26 and LB were higher (P<0.05) than that in CON and LP27. Lactate to acetate ratio was highest (P<0.05) in LB, but lowest in LB26. Lactic acid bacteria (LAB) count in LB was higher (P<0.05) than that in CON, while yeast count in CON was lower than in all silages applied inoculants. In conclusion, silages inoculated with LB26 could improve potentially the aerobic stability caused by increases of acetate and propionate concentrations.

Effects of Hybrid and Inoculant on Rumen Digestibility and Fermentation Indices of Corn Silage

Seong Shin Lee,Paradhipta H. V. Dimas,Young Ho Joo,Hyuk Jun Lee,Dong Hyeon Kim,Sang Bum Kim,김삼철 경상국립대학교 농업생명과학연구원 2019 농업생명과학연구 Vol.53 No.1

This study was aimed to determine the effects of hybrid and inoculant on in vitro rumendigestibility and rumen fermentation indices of corn silage. Two corn hybrids(Kwangpyeongok(KW) vs. Pioneer 1543(PI)) were ensiled for 100 days with two inoculants(L. plantarum(LP) vs. L. buchneri(LB)) applied at 1.2 × 105 cfu/g of fresh forage. Silages were incubated in rumenfluid medium at 39oC for 72 hours. Both of KW and PI had no difference(p>0.05) on in vitrodigestibility of dry matter(IVDMD) and neutral detergent fiber(IVNDFD), while application ofLB produced higher(p<0.05) IVDMD and IVNDFD than LP. Hybrid and inoculant applicationsdid not affect(p>0.05) total gas volume, ammonia-N, total VFA, and acetate concentrations inthe rumen. Applied LB was higher(p<0.05) propionate concentration than LP, while hybrids hadno effect(p>0.05). Furthermore, the interaction effect(p<0.05) was occurred in propionateconcentration. Butyrate concentration did not affected(p>0.05) by hybrid and inoculantapplications, but there was an interaction effect between hybrid and inoculant(p<0.05). Applications of hybrid and inoculant had effects(p<0.05) on iso-butyrate and valerate, withoutany interaction effects(p>0.05). This study concluded that application of KW and PI has noeffects on in vitro rumen digestibility and rumen fermentation indices of corn silage, whileapplication of LB increase the rumen digestibility in both of corn hybrids.