Deeper Wrinkle Formation and Less Melanin Production in Aged Korean Women with B Blood Type

( Jang-hee Oh ),( Inn-gyung Oh ),( Chi-hyun Park ),( Min Kyeong Shin ),( Se-rah Lee ),( Dong Hun Lee ),( Mira Choi ),( Seon-pil Jin ),( Hyun-sun Yoon ),( Soyun Cho ),( Jin Ho Chung ) 대한피부과학회 2018 Annals of Dermatology Vol.30 No.3

[6]-Shogaol inhibits melanogenesis in B16 mouse melanoma cells through activation of the ERK pathway

Yao, Cheng,Oh, Jang-hee,Oh, Inn Gyung,Park, Chi-hyun,Chung, Jin Ho Springer Science and Business Media LLC 2013 Acta pharmacologica Sinica. Vol.34 No.2

<P>Aim: To investigate the effect of [6]-shogaol, an active ingredient in ginger, on melanogenesis and the underlying mechanisms. Methods: B16F10 mouse melanoma cells were tested. Cell viability was determined with the MU assay. Melanin content and tyrosinase activity were analyzed with a spectrophotometer. The protein expression of tyrosinase and microphthalmia associated transcription factor (MITF), as well as phosphorylated or total ERK1/2 and Akt were measured using Western blot. Results: Treatment of the cells with [6]-shogaol (1, 5, 10 mu mol/L) reduced the melanin content in a concentration-dependent manner. [6]-Shogaol (5 and 10 mu mol/L) significantly decreased the intracellular tyrosinase activity, and markedly suppressed the expression levels of tyrosinase and MITF proteins in the cells. Furthermore, [6]-shogaol (10 mu mol/L) activated ERK, which was known to negatively regulate melanin synthesis in these cells. Pretreatment with the specific ERK pathway inhibitor PD98059 (20 mu mol/L) greatly attenuated the inhibition of melanin synthesis by [6]-shogaol (10 mu mol/L). Conclusion: The results demonstrate that [6]-shogaol inhibits melanogenesis in B16F10 mouse melanoma cells via activating the ERK pathway.</P>

P003 : Blood type B-specific difference in skin phenotypes of Korean women

( Jang Hee Oh ),( Inn Gyung Oh ),( Chi Hyun Park ),( Min Kyeong Shin ),( Serah Lee ),( Dong Hun Lee ),( Mira Choi ),( Seon Pil Jin ),( Soyun Cho ),( Jin Ho Chung ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: ABO blood group antigen expression was found on many tissues including the granular of the epidermis. Objectives: To investigate the blood type-specific difference in normal skin phenotypes in Korean women. Methods: Skin hydration, transepidermal water loss (TEWL), wrinkle depth, elasticity, or skin erythema/melanin index, were analyzed according to the blood type. Results: The skin hydration at buttock skin showed significantly lower in B blood type individuals than non-blood type B individuals of same young age range (20~49 yr, mean age; 34.0±9.3 yr, n=40), but TEWL of them showed no difference. However, the skin hydration and TEWL in old age range (54~84 yr, mean age; 70.4±6.2 yr, n=126) showed no B blood type-specific difference in inner-arm skin. The eye wrinkle depth in old B blood type individuals also was observed to be significantly deeper than non-B blood type individuals of same old age range. Deeper eye wrinkle was also observed in O blood type than A or AB blood type, but seemed less than B blood type. However, their skin elasticity showed no difference. The melanin index in old B blood type individuals also showed less measurement, which means bright skin color, than non-B blood type individuals, while the erythema index did not. Conclusion: In conclusion, we found that B blood type individuals probably has most distinct skin phenotypes, including less hydration, deeper eye wrinkle, and bright skin color in Korean women.

Stimulatory Effect of Dietary Red Ginseng on Epidermal Hydration and Ceramide Levels in Ultraviolet-Irradiated Hairless Mice

Kim, Hyun-Ae,Oh, Inn-Gyung,Park, Kyung-Ho,Kim, Na-Mi,Do, Jae-Ho,Cho, Yun-Hi The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.4

Ultraviolet (UV) irradiation induces skin dryness, largely by disruption of the epidermal barrier. In a search for dietary and plant compounds that would protect against skin dryness, we investigated the dietary effect of red ginseng (the steamed root of Panax ginseng C.A. Meyer) on epidermal levels of hydration and ceramides, the most important lipids for maintaining the epidermal barrier, in UV-irradiated mice. Albino hairless mice were fed either control diets (group UV [UV-irradiated control]) or diets with 0.5% (group H0.5) or 1% (group H1.0) red ginseng extract for 5 weeks in parallel with UV irradiation. A normal control group (group C) was fed a control diet without UV irradiation for 5 weeks. Skin dryness in group UV, as assessed by epidermal levels of hydration and ceramides, was significantly lower than those in group C. With no differences in food intake and weight gains among groups, epidermal levels of hydration and ceramides in group H0.5 were similar to those in group C. In addition, protein expression of serine palmitoyltransferase (SPT), a key enzyme involved in de novo ceramide synthesis, was increased in group H0.5. However, epidermal levels of hydration and ceramides in group H1.0 did not differ from those in group UV, in which ceramidase, an enzyme involved in ceramide degradation, was highly expressed. In conclusion, we demonstrate that dietary supplementation of 0.5% red ginseng protects skin from UV-induced dryness with an accumulation of ceramides due to elevated expression of SPT protein.

Melia azedarach extract stimulates melanogenesis through increase of tyrosinase-related protein 1 expression in B16F10 mouse melanoma cells.

Yao, Cheng,Jin, Cheng Long,Oh, Inn Gyung,Park, Chi-Hyun,Chung, Jin Ho UNKNOWN 2015 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.35 No.6

<P>Melia azedarach (MA) has been used in folk medicine in Asia for the treatment of several diseases. Several constituents from MA possess anti-herpetic, anti-angiogenic and anticancer properties. The aim of the present study was to investigate the effect of a 70% ethanol extract of MA on melanogenesis and the underlying mechanisms involved. A B16F10 mouse melanoma cell line was used in our experiments. Treatment of B16F10 cells with the MA extract (10, 20 and 40 ?g/ml) increased melanin content in a concentration-dependent manner without cytotoxicity at 24 h. Further experiments indicated that the MA extract (20 ?g/ml) increased melanin content as early as at 4 h after treatment. Additionally, although the MA extract did not affect intracellular tyrosinase activity and the protein levels of tyrosinase and tyrosinase-related protein-2 (TRP-2) at 2 and 4 h after treatment, the MA extract increased TRP-1 protein expression at both time points. However, no significant effect of the MA extract treatment on TRP-1 mRNA level at the time points measured was observed. In conclusion, the results from the present study demonstrate that the MA extract increases melanogenesis through the upregulation of TRP-1 protein expression by post-transcriptional control in B16F10 cells and suggest that the MA extract can be viewed as a rapid inducer of melanogenesis, thus rendering it a potential treatment for hypopigmentation diseases including vitiligo.</P>

Ardisia crenata extract stimulates melanogenesis in B16F10 melanoma cells through inhibiting ERK1/2 and Akt activation.

Yao, Cheng,Jin, Cheng Long,Oh, Jang-Hee,Oh, Inn Gyung,Park, Chi-Hyun,Chung, Jin Ho SPANDIDOS PUBLICATIONS 2015 MOLECULAR MEDICINE REPORTS Vol.11 No.1

<P>Melanin protects the skin against ultraviolet radiation by scattering incoming light and absorbing diverse free radicals. Agents that increase melanin synthesis in melanocytes may reduce the risk of photodamage and skin cancer. The present study investigated the effect of a methanol extract of Ardisia crenata (AC) on melanogenesis in B16F10 cells. Treatment of cultured B16F10 cells with AC extract (10, 20 and 40 ?g/ml) stimulated an increase in melanin levels in a concentration-dependent manner, without cytotoxicity. Tyrosinase is key in the regulation of melanin production, thus the effect of AC extract on tyrosinase activity and protein expression was analyzed. AC extract was observed to significantly increase tyrosinase activity and protein expression in B16F10 cells. Furthermore, AC extract was found to markedly increase the protein expression of microphthalmia-associated transcription factor, which is an important transcription factor involved in tyrosinase gene expression. In addition, AC extract (40 ?g/ml) was observed to suppress the activation of extracellular signal-regulated kinase (ERK) and Akt, which negatively regulate melanin synthesis in B16F10 cells. In conclusion, to the best of our knowledge, the present study is the first to show that a methanol extract of AC stimulates melanogenesis by increasing tyrosinase expression via the inhibition of ERK and Akt. Thus, methanol extract of AC may be a potential treatment for hypopigmentation diseases and may be a candidate for skin-tanning cosmetic products.</P>

자외선이 조사된 무모생쥐에서 홍삼, 사상자, 산수유 혼합 추출물 섭취가 표피 세라마이드 함량 및 관련 효소의 발현에 미치는 영향

이윤주(Lee Yun Ju),오인경(Oh Inn Gyung),조윤희(Cho Yunhi) 韓國營養學會 2012 Journal of Nutrition and Health Vol.45 No.3

UV-irradiation is a major factor of photo-aged skin, by which pigmentation, wrinkles and laxity are increased. In addition, the epidermal barrier is disrupted, ultimately causing dryness in photo-aged skin. As an effort to search dietary sources for improving the dryness of UV irradiated skin, the dietary effect of red ginseng based functional foods on the epidermal level of ceramides, a major lipid maintaining epidermal barrier, was determined in this study. Albino hairless mice were fed either a control diet [group UV (UV-irradiated control)] or diets with 0.5% (group M0.5) or 1% (group M1.0) of red ginseng extracts mixed with Torilis fructus and Corni fructus (66.7% red ginseng) in parallel with UV irradiation for 5 wks. A normal control group (group C) was fed a control diet without UV irradiation for 5 wks. The epidermal level of ceramides in group UV was significantly lower than that in group C, in which ceramidase, an enzyme involved in ceramide degradation, was highly expressed. In group M0.5, the epidermal level of ceramide was significantly increased to the level even higher than in group C. In addition, protein expression of serine palmitoyl transferase (SPT), a key enzyme involved in de novo ceramide synthesis, was increased in group M0.5. However the epidermal levels of ceramides as well as of ceramidase protein expression in group M1.0 did not differ from those in group UV. In conclusion, we demonstrate that dietary supplementation of red-ginseng extracts mixed with Torilis fructus and Corni fructus at a level of 0.5% level in diet increased the epidermal level of ceramides coupled with the elevated expression of SPT protein.

Oral Administration of KTNG0345 Prepared from Red Ginseng Extracts Reduces UVB-induced Skin Wrinkle Formation in Hairless Mice

Min Jung Lee,Chong Hyun Won,Se Rah Lee,Ji Sook Kim,Inn Gyung Oh,Euiil Hwang,Nami Kim,Byeong Choel Kang,Jin Ho Chung 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.1

Chronic ultraviolet (UV) exposure is the major cause of photoaging that causes skin wrinkling, roughness, dryness, laxity, and pigmentation. Recently, increasing efforts are being made to understand the relationship between foods and skin health. Ginsenosides are present in ginseng (Ginseng Radix Rubra) extract, and are known to have biomedical properties, such as, anti-oxidant and anti-inflammatory effects. In this study, we investigated whether KTNG0345 prepared from red ginseng extracts delivered orally reduces skin wrinkling and ultraviolet B (UVB)-induced wrinkle formation in hairless mouse skin. KTNG0345 was administrated orally to the mice (5 times a week) during the period of UVB-irradiation (3 times a week) for 8 weeks at three different doses of 300 ㎎/㎏, 500 ㎎/㎏ and 1000 ㎎/㎏ (w/v). UV doses were increased weekly by 1 MED (1MED = 75 mJ/㎠) up to 4 MED and then maintained at this level. After the 8-week administration period, it was found that orally administered KTNG0345 significantly inhibited UVB-induced wrinkle formation in a dose-dependent manner. Increases in skin thickness caused by UVB were prevented by KTNG0345. Moreover, it also significantly inhibited matrix metalloproteinases (MMP) -13 and MMP-9 expressional inductions by UVB. In addition, KTNG0345 was observed to prevent UVB-induced water loss of epidermis in hairless mouse skin. Our results demonstrate that orally administered KTNG0345 has anti-wrinkling effects in hairless mouse skin, and suggest that dietary red ginseng and herbal mixture may be considered a functional beauty food for preventing UVB-induced skin wrinkles.

Oral Administration of KTNG0345 Prepared from Red Ginseng Extracts Reduces UVB-induced Skin Wrinkle Formation in Hairless Mice

Lee, Min-Jung,Won, Chong-Hyun,Lee, Se-Rah,Kim, Ji-Sook,Oh, Inn-Gyung,Hwang, Eui-Il,Kim, Na-mi,Kang, Byeong-Choel,Chung, Jin-Ho The Korean Society of Ginseng 2008 Journal of Ginseng Research Vol.32 No.1

Chronic ultraviolet (UV) exposure is the major cause of photoaging that causes skin wrinkling, roughness, dryness, laxity, and pigmentation. Recently, increasing efforts are being made to understand the relationship between foods and skin health. Ginsenosides are present in ginseng (Ginseng Radix Rubra) extract, and are known to have biomedical properties, such as, anti-oxidant and anti-inflammatory effects. In this study, we investigated whether KTNG0345 prepared from red ginseng extracts delivered orally reduces skin wrinkling and ultraviolet B (UVB)-induced wrinkle formation in hairless mouse skin. KTNG0345 was administrated orally to the mice (5 times a week) during the period of UVB-irradiation (3 times a week) for 8 weeks at three different doses of 300 mg/kg, 500 mg/kg and 1000 mg/kg (w/v). UV doses were increased weekly by 1 MED (1MED = 75 $mJ/cm^2)$ up to 4 MED and then maintained at this level. After the 8-week administration period, it was found that orally administered KTNG0345 significantly inhibited UVB-induced wrinkle formation in a dose-dependent manner. Increases in skin thickness caused by UVB were prevented by KTNG0345. Moreover, it also significantly inhibited matrix metalloproteinases (MMP) -13 and MMP-9 expressional inductions by UVB. In addition, KTNG0345 was observed to prevent UVB-induced water loss of epidermis in hairless mouse skin. Our results demonstrate that orally administered KTNG0345 has anti-wrinkling effects in hairless mouse skin, and suggest that dietary red ginseng and herbal mixture may be considered a functional beauty food for preventing UVB-induced skin wrinkles.

제천지역의 향토음식에 관한 인지도 및 기호도 조사

민성희,엄정선,오인경,Min Sung-Hee,Um Jeong-Sun,Oh Inn-Gyung 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.5

The purpose of this study was to have a proper understanding and a concern about native local food in Jecheon area. The data was obtained by use of questionnaires to residents in Jecheon and statistical analysis was performed The results were as follows: 1. Deodeokgui got the highest score in perception, preference, and possibility of development as a native local food in Jecheon. 2. Hyangeobaeksuk and Memiljeonbyung are significantly different in perception by gender. Perception scores of native local food were not significantly different by increase of age and resident period The perception scores of Dotorichaemuk and Minmulbibimhoe by nuclear family member were significantly high compared with that by extended family members. The perception score of Deodeokgui increased as the monthly income increased 3. Female subjects liked Gondeurebap and Memiljeonbyung whereas male ones liked Hyangeobaeksuk and Minmulbibimhoe. Preferences of Torimyun, Dotorichaemuk, Kongtang, Hyangeobaeksuk, Chikyori, Gobonju Seungumchotteok were increased as the age increased 4. According to this study, modernization was required for development of native local food.