Effect of Different Packaging Atmosphere on Microbiological Shelf Life, Physicochemical Attributes, and Sensory Characteristics of Chilled Poultry Fillets

Kashif Nauman,Muhammad Hayat Jaspal,Bilal Asghar,Adeel Manzoor,Kumayl Hassan Akhtar,Usman Ali,Sher Ali,Jamal Nasir,Muhammad Sohaib,Iftikhar Hussain Badar 한국축산식품학회 2022 한국축산식품학회지 Vol.42 No.1

This trial was conducted to evaluate the effect of overwrap, vacuum, and modified atmosphere packaging (MAP) on poultry breast fillets' microbiological, biochemical shelf life and sensory attributes. The fillets were divided into 4 groups, and each of the treatments was replicated 3 times with 60 breast fillets. The first group was a control group with overwrap packaging; the second group was vacuum packed (VP); the third and fourth groups were MAP-1: 0% O2, 40% CO2, 60% N2, and MAP-2: 20% O2, 40% CO2, 40% N2. The microbiological and biochemical analyses were performed for the total viable count, coliform count, Pseudomonas count, Salmonella count, total volatile basic nitrogen (TVB-N), pH, cooking loss, color, lipid oxidation, tenderness, and sensory analysis. The data were analysed through two-way ANOVA by Minitab (Minitab 17.3.1). Meat treated with understudy MAP compositions and vacuum packaging reduced total viable count, Pseudomonas count, and total coliform count than control (p<0.05). TVB-N remained below the recommended limit throughout storage except aerobic packaging (p<0.05). Cooking loss (%) was lowered and showed non-significant results (p>0.05) between vacuum packaging and both MAP concentrations. The meat stored in MAP-2 was characterised by higher (p<0.05) visual scores. Whilst MAP-1 showed higher (p<0.05) L* values and overall acceptability. Sample packaged under aerobic packaging showed significant (p<0.05) results for b* and thiobarbituric acid reactive substances (TBARS). Meat stored in aerobic packaging showed higher (p<0.05) shear force values. The outcome of this trial may help to promote the application of understudy MAP compositions and rapid detection of microbes by biochemical analysis under local conditions.

Metabolites of Marine Algae Collected from Karachi-coasts of Arabian Sea

Muhammad Shaiq Ali,Muhammad Jahangir,Muhammad Saleem,Muhammad Kashif Pervez,Shaista Hameed,Viqar Uddin Ahmad 한국생약학회 2000 Natural Product Sciences Vol.6 No.2

The ethanolic extracts of marine green, brown and red algae collected from Karachi coasts of Arabian Sea afforded a new enol-derivative of N-acylsphingosine named as coelarthenol (1) from Coelarthrum muelleri, two new glucose-derivatives named: botryenal (2) and botryenol (3) from Botryocladia leptopoda, α-tocopherol quinone (4) from Codium iyengarii, β-sitosterol and hexadecanoic acid from Stokeyia indica. The known constituents (4, β-sitosterol & hexadecanoic acid) have not been reported so far from their corresponding sources and the structures were determined through spectroscopic methods, whereas, the structures of new constituents (1-3) were elucidated with the aid of selective HMBC experiments. The phytotoxicity of 4 was also monitored.

Assessment of Rheological and Piezoresistive Properties of Graphene based Cement Composites

Sardar Kashif Ur Rehman,Zainah Ibrahim,Mohammad Jameel,Shazim Ali Memon,Muhammad Faisal Javed,Muhammad Aslam,Kashif Mehmood,Sohaib Nazar 한국콘크리트학회 2018 International Journal of Concrete Structures and M Vol.12 No.6

The concrete production processes including materials mixing, pumping, transportation, injection, pouring, moulding and compaction, are dependent on the rheological properties. Hence, in this research, the rheological properties of fresh cement paste with different content of graphene (0.03, 0.05 and 0.10% by weight of cement) were investigated. The parameters considered were test geometries (concentric cylinders and parallel plates), shear rate range (300–0.6, 200–0.6 and 100–0.6 s<SUP>−1</SUP>), resting time (0, 30 and 60 min) and superplasticizer dosage (0 and 0.1% by weight of cement). Four rheological prediction models such as Modified Bingham, Herschel–Bulkley, Bingham model and Casson model were chosen for the estimation of the yield stress, plastic viscosity and trend of the flow curves. The effectiveness of these rheological models in predicting the flow properties of cement paste was verified by considering the standard error method. Test results showed that the yield stress and the plastic viscosity increased with the increase in graphene content and resting time while the yield stress and the plastic viscosity decreased with the increase in the dosage of superplasticizer. At higher shear rate range, the yield stress increased while the plastic viscosities decreased. The Herschel–Bulkley model with the lowest average standard error and standard deviation value was found to best fit the experimental data, whereas, Casson model was found to be the most unfitted model. Graphene reduces the flow diameter and electrical resistivity up to 9.3 and 67.8% and enhances load carrying capacity and strain up to 16.7 and 70.1% of the composite specimen as compared with plain cement specimen. Moreover, it opened a new dimension for graphene-cement composite as smart sensing building construction material.

Watermelon nutrition profile, antioxidant activity, and processing

Muhammad Nadeem,Maham Navida,Kashif Ameer,Farzana Siddique,Aqsa Iqbal,Faiqa Malik,Muhamad Modassar Ali Nawaz Ranjha,Zarina Yasmin,Rabia Kanwal,Sadaf Javaria 한국식품저장유통학회 2022 한국식품저장유통학회지 Vol.29 No.4

The first primitive watermelon domestication has been reported more than 5,000 years ago in Northeast Africa. Watermelon is recognized all over the world as a delicious fruit that quenches the thirst that many people consume in the summer heat. There are about 1,200 varieties of watermelon. Watermelons are loaded with plentiful nutrients, such as vitamin C, pantothenic acid, copper, biotin, vitamin A, and vitamins B6 & B1. Watermelon is processed to manufacture various value-added foods, such as cookies, cakes, juice, jam, cadies, and biscuits. In this review, we discuss pre- and postharvest factors affecting watermelon nutritional concentration and antioxidant profile. Prominent factors comprise genetic and environmental constraints, processing and postharvest storage conditions, chemical treatments, temperature and humidity, packaging, and food processing conditions. This review summarizes the available literature on watermelon processing into different valuable products, pre- and postharvest factors affecting nutritional concentration, and antioxidant activity.

Acacia Species for the Rehabilitation and Utilization of Salt-affected Lands and Waters

Muhammad Saqib,Ghulam Abbas,Javaid Akhtar,Muhammad Kashif Ali 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Germline Variations of Apurinic/Apyrimidinic Endonuclease 1 (APEX1) Detected in Female Breast Cancer Patients

Ali, Kashif,Mahjabeen, Ishrat,Sabir, Maimoona,Baig, Ruqia Mehmood,Zafeer, Maryam,Faheem, Muhammad,Kayani, Mahmood Akhtar Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Apurinic/apyrimidinic endonuclease 1 (APEX1) is a multifunctional protein which plays a central role in the BER pathway. APEX1 gene being highly polymorphic in cancer patients and has been indicated to have a contributive role in Apurinic/apyrimidinic (AP) site accumulation in DNA and consequently an increased risk of cancer development. In this case-control study, all exons of the APEX1 gene and its exon/intron boundaries were amplified in 530 breast cancer patients and 395 matched healthy controls and then analyzed by single-stranded conformational polymorphism followed by sequencing. Sequence analysis revealed fourteen heterozygous mutations, seven 5'UTR, one 3'UTR, two intronic and four missense. Among identified mutations one 5'UTR (rs41561214), one 3'UTR (rs17112002) and one missense mutation (Ser129Arg, Mahjabeen et al., 2013) had already been reported while the remaining eleven mutations. Six novel mutations (g.20923366T>G, g.20923435G>A, g.20923462G>A, g.20923516G>A, 20923539G>A, g.20923529C>T) were observed in 5'UTR region, two (g.20923585T>G, g.20923589T>G) in intron1 and three missense (Glu101Lys, Ala121Pro, Ser123Trp) in exon 4. Frequencues of 5'UTR mutations; g.20923366T>G, g.20923435G>A and 3'UTR (rs17112002) were calculated as 0.13, 0.1 and 0.1 respectively. Whereas, the frequency of missense mutations Glu101Lys, Ser123Trp and Ser129Arg was calculated as 0.05. A significant association was observed between APEX1 mutations and increased breast cancer by ~9 fold (OR=8.68, 95%CI=2.64 to 28.5) with g.20923435G>A (5'UTR), ~13 fold (OR= 12.6, 95%CI=3.01 to 53.0) with g.20923539G>A (5'UTR) and~5 fold increase with three missense mutations [Glu101Lys (OR=4.82, 95%CI=1.97 to 11.80), Ser123Trp (OR=4.62, 95%CI=1.7 to 12.19), Ser129Arg (OR=4.86, 95%CI=1.43 to 16.53)]. The incidence of observed mutations was found higher in patients with family history and with early menopause. In conclusion, our study demonstrates a significant association between germ line APEX1 mutations and breast cancer patients in the Pakistani population.

Ultra-trace level colorimetric composite sensor based on novel DH-1,6-NAPY-8-CN-AgNPs for the detection of Clonazepam in aqueous and human plasma samples

Shujaat Ali,Abdul Rehman Umar,Kashif Hussain,Haji Muhammad,Muddasir Hanif,Mouna Hind Laiche,Sufian Rasheed,Kousar Yasmeen,Abdul Hameed,Muhammad Raza Shah 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.125 No.-

It’s crucial to develop cost-effective, rapid & reliable detection methods of specific analytes through sensorsuseful in the analytical and medicinal chemistry. We report a highly sensitive and selective colorimetricmethod for the detection of clonazepam by the new composite sensor DH-1,6-NAPY-8-CNAgNPscontaining 5-amino-7-(4-benzylpiperazin-1-yl)-2,4-bis(4-bromophenyl)-2-methyl-1,3-dihydro-1,6-naphthyridine-8-carbonitrile capped silver nanoparticles (AgNPs). The detection mechanism is basedon the H-bonding interactions between clonazepam and the sensor, that caused the aggregation of NPsthat initiated a sharp color change from yellow to red. The linear relationship between the adjacentabsorbance values (DA) vs. clonazepam concentration (range = 0.05–75 lM) showed a correlation coefficientof 0.9927. The limit of detection (LOD) was 3 nM, that is very significant achievement over theexisting reports. The proposed sensor is highly selective, with no interference from many other possibleinterfering substances. The sensor was successfully applied to the aqueous and human plasma samples,therefore DH-1,6-NAPY-8-CN-AgNPs demonstrated great potential for the on-site and real-time screeningof Clonazepam.

Pharmacological effect of Rubus ulmifolius Schott as antihyperglycemic and antihyperlipidemic on streptozotocin (STZ)-induced albino mice

Khalil Akhtar,Syed Wadood Ali Shah,Assar Ali Shah,Muhammad Shoaib,Syed Kashif Haleem,Nighat Sultana 한국응용생명화학회 2017 Applied Biological Chemistry (Appl Biol Chem) Vol.60 No.4

The aim of present study was to evaluate the antihyperglycemic and antihyperlipidemic effects of aerial parts of Rubus ulmifolius Schott on streptozotocin (STZ)- induced diabetic albino mice. A total of 48-, 60-day-old either sex (male and female) albino mice were treated with, normal control; 2% Tween-80 suspension (diabetic control); glibenclamide (500 lg/kg/orally); RU methanol extract (150 mg/kg/orally) (RUCrd1); RU methanol extract (300 mg/kg/orally) (RUCrd2); RU chloroform extract (150 mg/kg/orally) (RUC); RU ethyl acetate extract (150 mg/kg/orally) (RUE); and RU butanol extract (150 mg/kg/orally) (RUB) for a period of 15 days. Diabetes was induced in albino mice by single intraperitoneal injection of streptozotocin (50 mg/kg/b/w). After 15 days, group treated with glibenclamide, RUCrd1, RUCrd2, RUC, RUE and RUB exhibited a significant (P[0.05) decrease in blood glucose level as compared to diabetic control groups. The total cholesterol, triglycerides and low-density lipoproteins as well as serum creatinine level, serum glutamate pyruvate transaminase, serum glutamate oxaloacetate transaminase and alkaline phosphatase were also significantly (P[0.05) decreased in glibenclamide, RUCrd1, RUCrd2, RUC, RUE and RUB groups albino mice as compared to diabetic control. It was concluded that Rubus ulmifolius Schott extract has positive effect as hypoglycemic and antihyperlipidemic on diabetic albino mice.

Orientation of Youth towards Social Entrepreneurship: An Empirical Study from Pakistan

Syed Sohaib ZUBAIR,Ifrah AYOOB,Kashif ALI,Mukaram Ali KHAN,Muhammad AZAD,Muhammad ZEESHAN 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.9

The importance of Entrepreneurship has been widely acknowledged by researchers and practitioners worldwide, however, the idea of Social Entrepreneurship is still considered to be an emerging area. Entrepreneurship is vital not only because of its economic impacts but also because it helps to address issues of poverty and welfare, where it can act as a catalyst for change. The importance of social entrepreneurship is that it serves to turn a profit and find success while helping others throughout the world. They know the power of social enterprise and are eager to serve the societal and economic benefits. This study aims to identify the level of orientation of youth towards social entrepreneurship in Pakistan. The study identifies the role of various factors that affect Social Entrepreneurial Orientation and is conducted following a quantitative research strategy and survey research design where data is collected from 302 individuals. Structural Equation Modelling was used to analyze the data and to test the hypotheses. The main finding of this research is that there is an increasing trend in the orientation towards social entrepreneurship. The exogenous variables namely Perceived Educational Support, Perceived Structural Support, and Perceived Relational Support were found to have positive and significant effects on the endogenous construct of Social Entrepreneurial Orientation.