Interferon-γ and Interleukin-10 Gene Polymorphisms are not Predictors of Chronic Hepatitis C (Genotype-4) Disease Progression

Bahgat, Nermine Ahmed,Kamal, Manal Mohamed,Abdelaziz, Ashraf Omar,Mohye, Mohamed Ahmed,Shousha, Hend Ibrahim,ahmed, Mae Mohamed,Elbaz, Tamer Mahmoud,Nabil, Mohamed Mahmoud Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.12

Immunoregulatory cytokines have an influence on hepatitis C virus (HCV) infection outcome. This study aimed to determine whether single nucleotide polymorphisms (SNP) in IFN- ${\gamma}$ and IL-10 genes are associated with susceptibility and/or are markers of prognosis regarding chronic hepatitis C outcomes. IFN ${\gamma}$ (+874T/A) and IL-10 (-1082G/A) genotypes were determined in 75 HCV genotype 4 patients with different disease severities (chronic hepatitis, n=25, liver cirrhosis and hepatocellular carcinoma (HCC) on top of liver cirrhosis, n=50) and 25 healthy participants using allele-specific polymerase chain reaction. No statistical differences in allele or genotype distributions of IFN ${\gamma}$ and IL-10 genes were detected between patients and controls or between patientgroups. No significant difference in the frequency of IL-10 SNP at position -1082 or IFN-${\gamma}$ at position +874T/A was found between chronic HCV genotype 4 and with progression of disease severity in liver cirrhosis or HCC. In conclusion; interferon-${\gamma}$ and interleukin-10 gene polymorphisms are not predictors of disease progression in patients with chronic hepatitis C (Genotype-4).

Environmentally benign Scouring of Cotton Knits Using Locally Produced Acid Pectinase Enzyme

Nabil A. Ibrahim,Basma M. Eid,Mohamed S. Abdel Aziz,Soha M. Hamdy,Safaa E. AbdAllah 한국섬유공학회 2019 Fibers and polymers Vol.20 No.4

The main task of the present study is to pre-screen selected fungal strain (Fusarium oxysporum M1-EGY) isolatedfrom local agriculture soil and optimize cultivation conditions for an acid pectinase production. Experimental resultsdemonstrated the optimum conditions for achieving the highest enzyme activity (14.80 U/ml) were found to be: incubationperiod (8 days) at pH (5), temperature (30 oC), and shaking rate (150 rpm), apple pectin carbon source (2 %), and ammoniumphosphate as nitrogen source (0.2 gN/l). The positive impacts of utilizing the produced crude acid-pectinase in eco-friendlyscouring of various cotton knits as well as their subsequent bleaching and reactive dyeing have been confirmed. Additionally,the use of local resources for an acid pectinase production would encourage and promote local production of textile enzymesand their potential applications in Egyptian textile sector, taking in consideration efficiency, textile quality, environmental andeconomy concerns.

Effectiveness of sequence-related amplified polymorphism (SRAP) markers to assess the geographical origin and genetic diversity of collected lentil genotypes

Mohammed Nabil A.,Afzal Muhammad,Al-Faifi Sulieman A.,Khan Muhammad A.,Refay Yahya A.,AL-Samin Bazel H.,Alghamdi Salem S.,Ibrahim Abdullah 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.4

Lentil is an important annual food legume crop, nitrogen fixer and provides a substantial amount of protein, carbohydrate, minerals, and vitamin content. The use of molecular markers to assess genetic diversity is crucial for crop improvement, efficient management, and conservation of plant genetic resources. The current study aimed to determine the genetic diversity among lentil genotypes using sequence-related amplified polymorphism (SRAP) markers. Therefore, we evaluated a collection of 36 lentil genotypes, including 20 from Yemen, Saudi Arabia (7), Egypt (4), and Bangladesh (3), and (2) genotypes from the International Center for Research in Dry Area (ICARDA) using 21 SRAP primer combinations. The amplified fragments showed a high level of useful polymorphic amplified fragments (775 out of 782) indicating a higher degree of variation. The polymorphic information content (PIC) ranged from 0.31 to 0.39 with an average of 0.33 for each primer. The UPGMA trees, based on Jaccard similarity index matrices, separated the genotypes into four main clusters according to their geographical origin. The population structure supported the major groups and attested to their great degree of differentiation. The highest lentil population was found at K = 3, K = 5, and K = 7 levels, showing purity and admixture ancestry among the lentil population. This study highlighted the wide genetic diversity among the studied lentil genotypes and demonstrated the effectiveness of the SRAP technique in determining the genetic variability of lentil. Furthermore, it could be used to establish the genetic peculiarity of ecotypes when applying for the obtainment of origin and agro-morphological characteristics.

Preemptive analgesic efficacy of ultrasound-guided transversalis fascia plane block in children undergoing inguinal herniorrhaphy: a randomized, double-blind, controlled study

Abdelbaser Ibrahim,Mageed Nabil A.,El-Emam El-Sayed M.,ALseoudy Mahmoud M.,Elmorsy Mohamed M. 대한마취통증의학회 2021 Korean Journal of Anesthesiology Vol.74 No.4

Background: Surgical repair of congenital inguinal hernia results in significant postoperative discomfort and pain. The aim of the current study was to evaluate the pre-emptive analgesic efficacy of a transversalis fascia plane (TFP) block after pediatric inguinal herniorrhaphy.Methods: Forty-four patients aged 12 to 60 months who underwent unilateral inguinal herniorrhaphy were enrolled. Four patients were excluded, and the remaining were allocated to the control group and the TFP block group. In the TFP block group, 0.4 mL/kg bupivacaine 0.25% was instilled in the plane between the transversus abdominis and transversalis fascia, while in the control group 0.9% saline was used instead of bupivacaine. The collected data were the total dose of paracetamol consumed during the first 12 h postoperatively, the postoperative Face, Leg, Activity, Cry, Consolability (FLACC) pain score, time to first use of rescue analgesia, number of patients required additional postoperative analgesics, and parents' satisfaction.Results: The median paracetamol consumption was significantly lower in the TFP block group than in the control group, and FLACC pain scores were significantly lower for all study times in the TFP block group with higher parental satisfaction scores than those for the control group. The number of patients who required additional analgesics was significantly lower in the TFP block group than in the control group. Conclusions: The use of a TFP block decreases postoperative analgesic consumption and postoperative pain intensity after pediatric inguinal herniorrhaphy. Future studies with larger sample size are required to evaluate the actual complications rate of TFP block.

The effect of ultrasound-guided bilateral thoracic retrolaminar block on analgesia after pediatric open cardiac surgery: a randomized controlled double-blind study

Abdelbaser Ibrahim,Mageed Nabil A.,Elfayoumy Sherif I.,Magdy Mohamed,Elmorsy Mohamed M.,ALseoudy Mahmoud M. 대한마취통증의학회 2022 Korean Journal of Anesthesiology Vol.75 No.3

Background: The thoracic retrolaminar block (TRLB) is a relatively new regional analgesia technique that can be used as an alternative to the thoracic paravertebral block. This study aimed to evaluate the postoperative analgesia effects of ultrasound-guided TRLB in children undergoing open cardiac surgery via median sternotomy incision.Methods: Sixty-six patients aged 2–8 years were recruited. In the TRLB group, 0.25% bupivacaine 0.4 ml/kg was injected into the retrolaminar space on both sides at the level of the T4 lamina. Patients in the control group were injected with 0.9% saline. The primary outcome measure was fentanyl consumption in the first 24 h post-extubation. The secondary outcome measures were the total intraoperative fentanyl consumption, postoperative modified objective pain score (MOPS), and time to extubation.Results: The total intraoperative fentanyl requirements and fentanyl consumption in the first 24 h post-extubation were significantly lower (P < 0.001) in the TRLB group (9.3 ± 1.2; 6.9 ± 2.1 μg/kg, respectively) than in the control group (12.5 ± 1.4; 16.6 ± 2.8, respectively). The median (Q1, Q3) time to extubation was significantly shorter (P < 0.001) in the TRLB group (2 [1, 3] h) than in the control group (6 [4.5, 6] h). The MOPS was significantly lower (P < 0.05) in the TRLB group than in the control group at 0, 2, 4, 8, 12 and 16 h post-extubation.Conclusions: Bilateral ultrasound-guided TRLB is effective in providing postoperative analgesia in children undergoing open cardiac surgery via median sternotomy incision.

A hypertension risk score for adults: a population-based cross-sectional study from Dubai Household Survey 2019

Mahmoud Ibrahim,Sulaiman Nabil,Hussein Amal,Mohammed Heba,AL Nakhi Wafa Khamis,Hussain Hamid,Ibrahim Gamal 한국역학회 2021 Epidemiology and Health Vol.43 No.-

OBJECTIVES: The aim of this study was to develop a risk score model for predicting hypertension specific to the population of Dubai in the United Arab Emirates (UAE) to facilitate prevention and early intervention. METHODS: A retrospective analysis of data from the Dubai Household Health Survey 2019 was conducted. Demographic and physical parameters, as well as blood glucose levels, were included in the data. The risk factors for hypertension were identified using bivariate analysis. A risk score model was developed using the enter method, where all significant predictors of hypertension in bivariate analyses were entered in a single step with the primary outcome of hypertension status (yes/no). The model was validated internally by splitting the data into Emirati and non-Emirati populations. RESULTS: A total of 2,533 subjects were studied. The significant risk factors for hypertension identified were male sex, older age (≥ 40 years), education level, body mass index, diabetes mellitus, and dyslipidaemia. The model showed a high discrimination ability between individuals with and without hypertension, with an area under the curve of 0.77 (95% confidence interval [CI], 0.75 to 0.79), excellent sensitivity (81.0%; 95% CI, 71.9 to 88.2) and moderate specificity (56.0%; 95% CI, 45.7 to 65.9). CONCLUSIONS: The model developed by this study is simple, convenient, and based on readily available demographic and medical characteristics. This risk score model could support initial hypertension screening and provide an effective tool for targeted lifestyle counselling and prevention programs.

Influence of some methylated hepatocarcinogenesis-related genes on the response to antiviral therapy and development of fibrosis in chronic hepatitis C patients

Waleed Seif Eldin Mohamed Mostafa,Mohammed Hassan Saiem Al-Dahr,Dalia Abdel Hamid Omran,Zeinab Fathy Abdullah,Suzan Hamdy Elmasry,Mohamed Nabil Ibrahim 대한간학회 2020 Clinical and Molecular Hepatology(대한간학회지) Vol.26 No.1

Background/Aims: Epigenetics involved in multiple normal cellular processes. Previous research have revealed the role of hepatitis C virus infection in accelerating methylation process and affecting response to treatment in chronic hepatitis patients. This work aimed to elucidate the role of promoter methylation (PM) in response to antiviral therapy, and its contribution to the development of fibrosis through hepatocarcinogenesis-related genes. Methods: A total of 159 chronic hepatitis Egyptian patients versus 100 healthy control group were included. The methylation profile of a panel 9 genes (SFRP1, p14, p73, APC, DAPK, RASSF1A, LINE1, O6MGMT, and p16) was detected in patients’ plasma using methylation-specific polymerase chain reaction (MSP). Results: Clinical and laboratory findings were gathered for patients with combined pegylated interferon and ribavirin antiviral therapy. Regarding the patients’ response to antiviral therapy, the percentage of non-responders for APC, O6MGMT, RASSF1A, SFRP1, and p16 methylated genes were significantly higher versus responders (P<0.05). Of the 159 included patients, the most frequent methylated genes were SFRP1 (102/159), followed by p16 (100/159), RASSF1A (98/159), then LINE1 (81/159), P73 (81/159), APC (78/159), DAPK (66/159), O6MGMT (66/159), and p14 (54/159). A total of 67/98 (68.4%) cases of RASSF1A methylated gene (P=0.0.024), and 62/100 (62%) cases of P16 methylated gene (P=0.03) were associated with mild-degree fibrosis. Conclusions: To recapitulate, the PM of SFRP1, APC, RASSF1A, O6MGMT, and p16 genes increases in chronic hepatitis C patients, and can affect patients’ response to antiviral therapy. The RASSF1A and P16 genes might have a role in the distinction between mild and marked fibrosis.