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Lee, Dahee,Choi, Eunji,Lee, Jaewon,Oh, Jungju,Lee, Seonyeong,Lee, Minhyung THE KOREAN SOCIETY OF INDUSTRIAL AND ENGINEERING 2018 JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY -S Vol.67 No.-
<P><B>Abstract</B></P> <P>Receptor for advanced glycation end-products (RAGE) is overexpressed in various cancer cells. In this study, a RAGE-binding peptide (RBP) was conjugated to polyethylenimine (25kDa, PEI). RBP-conjugated PEI (PEI-RBP) was characterized as a dual-functional reagent, a RAGE-mediated gene carrier and an anti-angiogenic reagent. As a gene carrier, PEI-RBP had higher transfection efficiency to the C6 glioblastoma cells than PEI. As an anti-angiogenic reagent, the pEmpty/PEI-RBP complex reduced RAGE expression on the surface of the C6 glioblastoma cells. Also, the complex reduced the VEGF expression and tube formation of endothelial cells. Therefore, PEI-RBP may be useful for development of glioblastoma therapy.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Seasonal abundance of mosquito species in diverse habitat types in Gyeonggi-do, South Korea
Minhyung Jung,Po-Hyun Park,Young-Ran Joo,Lee-Hyeon Sin,Uk-Gyo LeeUk-Gyo Lee,Doo-Hyung Lee 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10
Mosquitoes are vectors of many human diseases such as Japanese encephalitis, vivax malaria, west nile fever, dengue fever and yellow fever. However, factors affecting the abundance and distribution of mosquitoes are not well-understood. Moreover, the global climate change makes it even more difficult to address and predict the population dynamics of mosquitoes. Therefore, it is important to monitor this medically-important pest throughout landscapes over a long period of time. This study was conducted as a part of the nation-wide surveillance program to monitor insect vectors. In 2015, mosquitoes were collected using black light traps and BG-Sentinel traps in Gyeonggi-do at every two weeks from April to September. Three types of potential mosquito habitats were selected for surveillance: urban area (Suwon), cattle shed (Yongin) and wild bird refuge (Gwangju). Among the three habitats, female mosquitoes were first caught on April 4 by BG-Sentinel trap in the urban area. The highest density of female mosquitoes in the traps was recorded in July in the urban area (134 ± 84 per trap); the highest density was observed in June in the cattle shed (2473 ± 1319) and wild bird refuge (21 ± 15) (mean ± SE). The dominant mosquito species were Culex pipens pallens (91%), Aedes vexans nipponii (79%) and Anopheles sinensis (33%) in the urban area, cattle shed and wild bird refuge, respectively. The results of this study indicate that female mosquitoes were most abundant in the cattle shed habitat type. It is also noteworthy that the dominant mosquito species varied among the habitat types surveyed. This nation-wide monitoring program for medical pests will provide fundamental basis to develop effective management strategy under the global climate change environment.
Minhyung Jung,Po-Hyun Park,Young-Ran Joo,Lee-Hyeon Sin,Uk-Gyo Lee,Doo-Hyung Lee 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04
Seasonal abundance and distribution of mosquitoes and chigger mites are important for human populations. However, factors affecting the abundance and distribution of vector species are not well - understood. Furthermore, global climate change makes it difficult to predict the population dynamics of vector species. Therefore, it is important to monitor this vector species over a long period of time. In 2015, mosquitoes were collected from three potential mosquito habitats using black light trap and BG-Sentinel trap in Gyeongi-do in April through October. Also, we collected chigger mite using sherman live traps in Cheorwon, Gangwon-do in April, September and October. From the survey, we collected a total of 11,649 mosquitoes in three potential mosquito habitats, and a total of 3,656 chigger mites from 54 wild rodents. In the samples, dominant mosquito species were Aedes vexans nipponii (Species Ratio 70%), Culex pipens pallens (SR 9.8%) in Gyeonggi area and dominant chigger mite species was Leptotrombidium pallidium (SR 59%) in Cheorwon. The results of this study can serve as provide fundamental basis to develop effective management strategy under the global climate change environment.
BES를 이용한 연동형 온실의 냉 · 난방 부하 산정 및 PV 시스템 발전 성능 분석
이민형(Minhyung Lee),이인복(In-Bok Lee),하태환(Tae-Hwan Ha),김락우(Rack-Woo Kim),여욱현(Uk-Hyeon Yeo),이상연(Sang-Yeon Lee),박관용(Gwanyong Park),김준규(Jun-Gyu Kim) (사)한국생물환경조절학회 2017 생물환경조절학회지 Vol.26 No.4
The price competitiveness of photovoltaic system (PV system) has risen recently due to the growth of industries, however, it is rarely applied to the greenhouse compared to other renewable energy. In order to evaluate the application of PV system in the greenhouse, power generation and optimal installation area of PV panels should be analyzed. For this purpose, the prediction of the heating and cooling loads of the greenhouse is necessary at first. Therefore, periodic and maximum energy loads of a multi-span greenhouse were estimated using Building Energy Simulation(BES) and optimal installation area of PV panels was derived in this study. 5 parameter equivalent circuit model was applied to analyzed power generation of PV system under different installation angle and the optimal installation condition of the PV system was derived. As a result of the energy simulation, the average cooling load and heating load of the greenhouse were 627,516MJ and 1,652,050MJ respectively when the ventilation rate was 60AE·hr<SUP>-1</SUP>. The highest electric power production of the PV system was generated when the installation angle was set to 30°. Also, adjustable PV system produced about 6% more electric power than the fixed PV system. Optimal installation area of the PV panels was derived with consideration of the estimated energy loads. As a result, optimal installation area of PV panels for fixed PV system and adjustable PV system were 521m² and 494m² respectively.
Instability of Electrically Driven Polymer Liquid Jets
Minhyung Lee,Seung Baik Kang,Joo Hyuk Park 대한기계학회 2006 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.20 No.3
Polymer nanofibers can be generated by a electrospinning process. The process involves electrically charged jet of polymer solutions evolving from a droplet. The jet stretches in vertical direction due to the difference between charged particle and constant current located at the collector, while the Coulomb and viscoelastic forces start to contribute to radial and azimuthal (torsional stretching. In this paper, the unstable dynamics of the liquid polymer jet is examined experimentally and theoretically. A complex viscoelastic rheological model has been adopted to analyze the behavior of a charged liquid jet. The model includes complex phenomena of stress relaxation of the liquid jet resulting from the competing force components. The experimental data of the jet paths captured by high-speed videocamera also confirm the similar behavior with the predictions.
Efficient exogenous DNA-free reprogramming with suicide gene vectors
Minhyung Lee,Jeongmin Ha,Ye Seul Son,Hyunjun Ahn,정광보,손미영,김장환 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Reprogramming with episomal vectors is an easy, safe, and cost-effective method to generate exogenous DNA-free (exogene-free) induced pluripotent stem cells (iPSCs). However, the genomic integration of exogenes is observed occasionally. Additionally, the removal of episomal DNA takes more than 70 days in established iPSCs. Here, we inserted the cytosine deaminase (CD) gene from yeast into episomal vectors and used them to reprogram human fibroblasts into iPSCs. These new episomal vectors (CD episomal vectors) were eliminated from the generated iPSCs as early as seven days after 5-fluorocytosine (5-FC) treatment. We also found that cells with the integration of the CD gene perished within two days of 5-FC treatment. In addition, we generated exogene-free induced neural stem cells after one passage by direct reprogramming with CD episomal vectors combined with 5-FC treatment. Conclusively, our novel method allows the rapid and easy isolation of exogene-free reprogrammed cells and can be applied to disease modeling and clinical applications.
Survey of overwintering sites of R. pedestris in diverse landscapes in Korea
Minhyung Jung,Doo-Hyung Lee 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.04
R. pedestris is a major insect pest in agricultural production, especially on soybean and tree fruit, in Korea. Currently, the management of this pest relies mainly on insecticide application in most commodities. However, the chemical controls cause numerous environmental problems. To develop sustainable management tools, it is fundamental to understand the basic biology and ecology of R. pedestris. However, the overwintering ecology of this pest is virtually unknown. In this study, we surveyed diverse landscapes including high elevation mountains soybean fields and there neighboring forests to find and characterize the overwintering sites of R. pedestris. In the field, we sampled leaf litters from 1㎡ grids to locate overwintering R. pedestris. The samples were taken every 100m altitude in the mountain areas or every 50m distance in the flat landscapes. Overwintering R. pedestris were found from 6 samples out of 137 samples. All overwintering R. pedestris were located from forested areas adjacent to urban landscapes in Seong-Nam si, Gyeong-Gi Do. Interestingly, no overwintering R. pedestris was found from in the samples collected from high elivation mountains including Yeon-In, Song-Ni, Chi-Ak, Ga-Ya, Wol-Chul. likewise, no overwintering individual was found from soybean fields and their neighboring forested areas. With caveat that the data were collected only for 1 year, our results support that R. pedestris overwinters solitary resulting in low detection rates from the samples. Given that R. pedestris typically disperse into cultivated crops with high densities, it is important to better understand environmental factors affecting the presence or absence of overwintering R. pedestrris in diverse landscapes. The knowledge would be essential to develop sustainable monitoring and management programs for this economically important pest in Korea.