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Preparation of CdSe QDs-carbohydrate Conjugation and its Application for HepG2 Cells Labeling
Jiang, Mingxing,Chen, Yan,Kai, Guiqing,Wang, Ruijun,Cui, Huali,Hu, Meili Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.2
In present study, CdSe quantum dots (QDs) were prepared with a novel but simple, effective and exercisable method. Nine different types of carbohydrate molecules were used to modify CdSe QDs. D-mannose (Man)-coated quantum dots were prepared for labeling human hepatoma (HepG2) cells, because of the high expression of mannose receptor (MR) on HepG2 cells. The uptake characteristics of CdSe QDs-Man were investigated in HepG2 cells. The absorption rate result of MTT assay in 48 h suggested the extremely low cytotoxicity of CdSe QDs-Man. The presence of quantum dots was confirmed with fluorescence microscopy. These results were encouraging regarding the application of QDs molecules for early detection of HepG2 cells.
Preparation of CdSe QDs-carbohydrate Conjugation and its Application for HepG2 Cells Labeling
Mingxing Jiang,Yan Chen,Guiqing Kai,Ruijun Wang,Huali Cui,Meili Hu 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.2
In present study, CdSe quantum dots (QDs) were prepared with a novel but simple, effective and exercisable method. Nine different types of carbohydrate molecules were used to modify CdSe QDs. D-mannose (Man)-coated quantum dots were prepared for labeling human hepatoma (HepG2) cells, because of the high expression of mannose receptor (MR) on HepG2 cells. The uptake characteristics of CdSe QDs-Man were investigated in HepG2 cells. The absorption rate result of MTT assay in 48 h suggested the extremely low cytotoxicity of CdSe QDs-Man. The presence of quantum dots was confirmed with fluorescence microscopy. These results were encouraging regarding the application of QDs molecules for early detection of HepG2 cells.
Identification and Characterization of EDT1 Conferring Drought Tolerance in Rice
Tao Wu,Mingxing Zhang,Hongjia Zhang,Kai Huang,Mojun Chen,Chen Chen,Xue Yang,Zhao Li,Haoyuan Chen,Zhiming Ma,Xunming Zhang,Wenzhu Jiang,Xinglin Du 한국식물학회 2019 Journal of Plant Biology Vol.62 No.1
Basic Leucine Zipper (bZIP) transcription factors(TFs) play important roles in many processes, especially inabiotic stress response in plants. In this study, we characterizeda new gene EHANCED DROUGHT TOLERANCE 1 (EDT1),member of group E of bZIP transcription factor family inrice. The EDT1 protein contains one bZIP domain, oneputative nuclear localization signal (NLS) and six conservedphosphorylation sites. The expression of EDT1 is suppressedby several abiotic stresses, such as cold, droutht, and salt. Corresponding with expression patterns, several stress-associatedcis-acting elements were found in the EDT1 promoter. Theresults of subcellular localization and transactivation abilityanalyses indicated that EDT1 was localized in the nucleusand functioned as a nuclear protein, with its transactivationactivity primarily located in N-terminal. Transgenic riceoverexpressing EDT1 showed drought tolerance that hasbeen significantly improved. Real-time PCR analysis revealedthat some stress-related genes, such as OsbZIP12, SNAC1,OsLEA3, OsbZIP16, OsbZIP10 and OsABI2 were up-regulatedin EDT1 overexpression lines. These results indicate thatEDT1 plays a positive role in drought tolerance and providesvaluable targets for breeding drought-tolerant rice cultivars.
Junyu Tian,Guang Xu,Zhengyi Jiang,Haijiang Hu,Mingxing Zhou 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.6
The effects of Nickle (Ni) addition on bainitic transformation and property of ultrahigh strength bainitic steels are investigatedby three austempering processes. The results indicate that Ni addition hinders the isothermal bainite transformation kinetics,and decreases the volume fraction of bainite due to the decrease of chemical driving force for nucleation and growth ofbainite transformation. Moreover, the product of tensile strength and total elongation (PSE) of high carbon bainitic steelsdecreases with Ni addition at higher austempering temperatures (220 and 250 °C), while it shows no significant differenceat lower austempering temperature (200 °C). For the same steel (Ni-free or Ni-added steel), the amounts of bainite and RAfirstly increase and then decrease with the increase of the austempering temperature, resulting in the highest PSE in thesample austempered at temperature of 220 °C. In addition, the effects of austempering time on bainite amount and propertyof high carbon bainitic steels are also analyzed. It indicates that in a given transformation time range of 30 h, more volumeof bainite and better mechanical property in high carbon bainitic steels can be obtained by increasing the isothermal transformationtime.