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      • KCI등재
      • KCI등재

        Surgical treatment for hepatocellular carcinoma with bile duct invasion

        Xu-guang Hu,Wei Mao,Sung Yeon Hong,Bong-Wan Kim,Wei-guang Xu,Hee-Jung Wang 대한외과학회 2016 Annals of Surgical Treatment and Research(ASRT) Vol.90 No.3

        Purpose: There is still some debate on surgical procedures for hepatocellular carcinoma (HCC) patients with bile duct tumor thrombi (BDTT, Ueda type 3 or 4). What is adequate extent of liver resection for curative treatment? Is extrahepatic bile duct resection mandatory for cure? The aim of this study is to answer these questions. Methods: Between February 1994 and December 2012, 877 consecutive HCC patients underwent hepatic resection at Ajou University Hospital. Thirty HCC patients (3.4%) with BDTT (Ueda type 3 or 4) were retrospective reviewed in this study. Results: In total, 20 patients enrolled in this study were divided into 2 groups: patients who underwent hemihepatectomy with extrahepatic bile duct resection (group 1, n = 10) and with only removal of BDTT (group 2, n = 10). The 1-, 3- and 5-year overall survival rates were 75.0%, 50.0%, and 27.8%, respectively. The 1-, 3-, and 5-year survival rates of group 1 were 100.0%, 80.0%, and 45.7%, and those of group 2 were 50.0%, 20.0%, and 10.0%, respectively (P = 0.014). The 1-, 3-, and 5-year recurrences free survival rates of group 1 were 90.0%, 70.0%, and 42.0%, and those of group 2 were 36.0%, 36.0%, and 0%, respectively (P = 0.014). Thrombectomy and infiltrative growth type (Ig) were found as independent prognostic factors for recurrence free survival by multivariate analysis. Thrombectomy, Ig, and high indocyanine green retention rate at 15 minutes were found as independent prognostic factors for overall survival by multivariate analysis. Conclusion: We suggest that the appropriate surgical procedure for icteric HCC patients should be comprised of ipsilateral hemihepatectomy with caudate lobectomy and extrahepatic bile duct resection.

      • SCOPUSKCI등재

        Amperometric Determination of Nitrite at Poly(Methylene Blue)-Modified Glassy Carbon Electrode

        Xu, Guang-Ri,Xu, Guifang,Xu, Ming-Lu,Zhang, Zhengqing,Tian, Yuan,Choi, Han-Nim,Lee, Won-Yong Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.2

        Electrochemical characteristics of nitrite ion were investigated at a poly(methylene blue)-modified glassy carbon electrode by cyclic voltammetry and differential pulse voltammetry. The poly(methylene blue)-modified glassy carbon electrode exhibited enhanced anodic signals for nitrite. The effects of key parameters on the detection of nitrite were evaluated at the modified electrode, such as pH, accumulation time, and scan rate. Under optimum condition, the chemically modified electrode can detect nitrite in the concentration range $2.0{\times}10^{-6}$ to $5.0{\times}10^{-4}$ M with the detection limit of $2.0{\times}10^{-6}$ M and a correlation coefficient of 0.999. The detection of nitrite using the chemically modified electrode was not affected by common ions such as $Na^+$, $K^+$, $Ca^{2+}$, $Cl^-$, $HPO_4^{2-}$ and $H_2PO_4^- $. The modified electrode showed good stability and reproducibility. The practical application of the present method was successfully applied to the determination of nitrite ion in cabbage samples.

      • KCI등재

        Amperometric Determination of Nitrite at Poly(Methylene Blue)-Modified Glassy Carbon Electrode

        Guang-Ri Xu,Guifang Xu,Ming-Lu Xu,Zhengqing Zhang,Yuan Tian,최한님,이원용 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.2

        Electrochemical characteristics of nitrite ion were investigated at a poly(methylene blue)-modified glassy carbon electrode by cyclic voltammetry and differential pulse voltammetry. The poly(methylene blue)-modified glassy carbon electrode exhibited enhanced anodic signals for nitrite. The effects of key parameters on the detection of nitrite were evaluated at the modified electrode, such as pH, accumulation time, and scan rate. Under optimum condition, the chemically modified electrode can detect nitrite in the concentration range 2.0 × 10−6 to 5.0 × 10−4 M with the detection limit of 2.0 × 10−6 M and a correlation coefficient of 0.999. The detection of nitrite using the chemically modified electrode was not affected by common ions such as Na+, K+,Ca2+, Cl−, HPO42− and H2PO4−. The modified electrode showed good stability and reproducibility. The practical application of the present method was successfully applied to the determination of nitrite ion in cabbage samples.

      • KCI등재

        RNAi suppression of nuclear receptor genes results in increased susceptibility to sulfoxaflor in brown planthopper, Nilaparvata lugens

        Lu Xu,Chun-Qing Zhao,De-Jin Xu,Guang-Chun Xu,Xiao-Long Xu,Zhao-Jun Han,Ya-Nan Zhang,Zhong-Yan Gu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

        Nuclear receptors (NRs), which belong to a superfamily of ligand-dependent transcription factors, play essential roles in gene regulation to affect numerous physiological pathways. Twenty NR genes were identified in Nilaparvata lugens by using genomic, transcriptomic and GenBank databases, and categorized into NR0-NR6 subfamilies according to standard nomenclature. Among them, three NR geneswere classed into NR0, four into NR1, eight into NR2, one into NR3, one into NR4, two into NR5, and one into NR6, respectively. A phylogenetic tree of NRs from N. lugens and other representative species was constructed, which provided evolutionary insight into genetic distance. In order to investigate the NRs,whichwere induced by sulfoxaflor, time- and tissue-specific expression profiles of NR genes in fourth-instar nymphs were determined following LD50 sulfoxaflor treatment (0.28 ng/insect) and compared with control samples. NlUSP, NlE78, NlTLL, NlHR51, NlHR83, NlPNR and NlFTZ-F1 were highly expressed following 12–48 h of sulfoxaflor treatment. NlHR39 and NlDSF transcripts were detected in the head and the thorax, respectively. High-level and constitutive expression of NlHR3, NlTLL, NlHR83, NlFTZF1 and NlHR4 were found in the abdomen. Feeding of dsRNAs reduced the expression of NlHR3, NlUSP, NlTLL, NlHR83, NlPNR, NlFTZ-F1 and NlHR4 (35.48–49.77%) and caused significant nymph mortality (69.21–81.45%). These NRs, considered as insecticide targets, may play important function in sulfoxaflor detoxification.

      • KCI등재후보

        Anti-inflammatory and Anti-oxidant Effects of PHBV/Collagen (PHCP) on Lipopolysaccharide-Induced Skin Inflammation

        Xu Zi Guang,Jyung-Sik Kwak,Ki Wan Oh,배한익,한진이 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.1

        The anti-inflammatory effect of PHBV/Collagen (PHCP) was examined in a mouse model of lipopolysaccharide (LPS)-induced skin inflammation. Vascular permeability on the back skin was measured by the local accumulation of evan’s blue dye after subcutaneous injection of LPS (30 μg site-1). Dye leakage in the skin was significantly increased 2 h after injection of LPS. This LPS-induced dye leakage was also completely inhibited by HO-1 inhibitor, ZnPP, and antioxidants, including methyl gallate, trolox, mannitol. To study the possible mechanisms underlying the in vivo anti-inflammatory effect of PHCP against LPS-induced inflammation, we also examined the effects of PHCP on malondialdehyde (MDA) and glutathione levels in skin tissues and found that PHCP pretreatment inhibited MDA elevation and the remarkable reduction of glutathione level. In addition, similar results were obtained after pretreatment with antioxidants such as trolox and mannitol and HO-1 inhibitor, ZnPP. Histopathologically, an influx of neutrophils into the skin dermis were detected between 24 h and 72 h after LPS injection (30, 100 μg site-1), compared to control animals after the injection of saline. This increase was greater in mice treated with 100 μg of mice than those of the 30 μg of mice and was significantly suppressed by pretreatment with PHCP, antioxidants and HO-1 inhibitor. These results collectively suggest that PHCP has anti-inflammatory effect against LPS-induced inflammation model in vivo and may be a good candidate for the skin tissue engineering biomedical application primarily through manipulation of the redox state.

      • Study on Efficiency Optimizing of PMSM for Pump Applications

        Guang-xu Zhou,Hui-jun Wang,Dong-Hee Lee,Jin-Woo Ahn 전력전자학회 2007 ICPE(ISPE)논문집 Vol.- No.-

        In view of the operation characteristics of pump application, pump system should have high efficiency and energy saving during the whole operating process. A novel efficiency-optimization control strategy is presented to meet the demand of high efficiency of variable speed high efficiency PMSM. The core of this strategy is the excellent integration of mended maximum torque to current control algorithm, which is based on the losses model during the dynamic and the grade search method with changed step by fuzzy logic during the steady. A TMS320LF2407A DSP-based digital control inverter for variable speed high efficiency PMSM is developed. The performance experiments for control system of variable speed high efficiency PMSM have been completed. The tested result verified that the system can reliably operate with the different control strategy during the dynamic and steady operation, and the system has more perfect performances using the efficiency-optimization control.

      • KCI등재후보

        Construction of Modified Bacillus thuringiensis cry1Ac Genes for Transgenic Crop Through Multi Site-directed Mutagenesis

        Xu, Hong Guang,Roh, Jong-Yul,Wang, Yong,Choi, Jae-Young,Shim, Hee-Jin,Liu, Qin,Tao, Xueying,Woo, Soo-Dong,Jin, Byung-Rae,Je, Yeon-Ho Korean Society of Sericultural Science 2009 International Journal of Industrial Entomology Vol.19 No.1

        The newly cloned Bacillus thuringiensis cry1-5 gene showed high activity to both Plutella xylostella and Spodoptera exigua, while cry1Ac only showed high activity against P. xylostella but low to S. exigua. Through the alignment of amino acid sequences between Cry1Ac and Cry1-5, we found 12 different residues in domain I (6 residues) and domain II (6 residues). In this study, the modified cry1Ac gene, which is constructed according to a crop-preferring codon usage, was used as a template to construct mutant B. thuringiensis cry1Ac genes based on cry1-5 gene through multi site-directed mutagenesis. Total 63 various mutant cry genes were obtained at 12 positions randomly. Among them, ten mutant cry genes, whose domain I was totally converted and domain II was randomly, were selected to express in baculovirus expression system as a polyhedrin fusion form. The recombinant proteins were 95 kDa in size and were stably activated as 65 kDa by trypsin. The expressed mutant Cry proteins were applied to bioassays against P. xylostella and S. exigua. All mutants showed high insecticidal activity both to P. xylostella and S. exigua similar to cry1-5. These results suggest that these mutant cry genes might be expected of desirable cry genes for introduction to transgenic crops.

      • SCISCIESCOPUS
      • A Tunable Combline Bandpass Filter Loaded With Series Resonator

        Xu-Guang Wang,Young-Ho Cho,Sang-Won Yun IEEE 2012 IEEE transactions on microwave theory and techniqu Vol.60 No.6

        <P>This paper proposes a varactor-tuned microstrip combline bandpass filter (BPF) that is loaded with lumped series resonators instead of the short-circuited end of combline. This configuration has the advantage of enhancing the tunability of the suggested BPF by varactor diodes in series resonators, which leads to a wide tuning range even with a small capacitance ratio (<I>C</I><SUB>ratio</SUB>) of the varactor. Therefore, a low controlled bias voltage varactor-tuned BPF can be achieved. In addition, due to the controllable slope parameter of the proposed resonator, the coupling between resonators can be controlled. As a result, the proposed tunable BPF can keep nearly constant bandwidth over the wide tuning range. The insertion loss resulting from the varactors is compensated using an active capacitance circuit with negative resistance. Theoretical analysis and design approach are described. Experimental results of a demonstrative BPF show a 500-MHz tuning range (1.7-2.2 GHz) with <I>C</I><SUB>ratio</SUB> of only 2.6 (1-5-V bias voltage) while maintaining nearly constant absolute bandwidth and low insertion loss. The measured performance of the fabricated filter shows good agreement with the simulated one.</P>

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