Mito-Tempo Can Protect Against the Allergic Airway Inflammation through Reducing Mitochondrial ROS Generation

( So Ri Kim ),( Yong Chul Lee ),( Dong Im Kim ),( Yang Keun Rhee ),( Heung Bum Lee ),( Seoung Ju Park ),( Chi Ryang Chung ),( Seung Yong Park ),( Mi Ran Kang ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

Oxidative stress is well known to be implicated in the development of asthma. The mitochondrial respiratory chain is a major site of intracellular reactive oxygen species (ROS) generation and, at the same time, an important target for the damaging effects of ROS. Mito-Tempo is a specific mitochondrial ROS inhibitor and it is known to be associated with opening of mi-tochondrial permeability transition pore and inhibition of cell necroptosis or apoptosis. However, there is little information on the protective effects of Mito-Tempo on the inflammatory airway disorders including bronchial asthma and its acute exacerbation. We investigate the effects of Mito-tempo on the allergic airway inflammation and hyperresponsiveness using the mice sensitized with OVA and LPS and then challenged with OVA (OVALPS-OVA mice). The OVALPS-OVA mice showed the typical features of neutrophilic asthma; increased airway inflammatory cells, the pathologic changes, the increased levels of Th2 cytokines in lungs of OVALPS-OVA mice, increased mitochondrial ROS generation, and increased bronchial hyperresponsiveness. Interestingly, we found that in OVALPS-OVA mice, Mito-Tempo, a novel mitochondrial targeting agent significantly reduced the increases in inflammatory cytokines, mitochondrial ROS generation, airway inflammation, and bron-chial hyperresponsiveness. These findings indicate that mitochondrial dysfunction including oxidative damage may be im-plicated in the pathogenesis of bronchial asthma and provide the therapeutic potential of a mitochondrial targeting agent, Mito-Tempo, for bronchial asthma.

Insectisidal activity and feeding behavior of green peach aphid, Myzus persicae against bio-nano type of Pyrifluqinazon

Kang, Min Ah,Kyu Sik Yoon,Hye Ri Kwon,Min Woo Park,Shin Hyuk Jo,Shin Hyo Seob,Sae Hee Kim,Eun Jin Kang,Mi Ja Seo,Yong Man Yu,Young Nam Youn 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10

Pyrifluquinazon, as a quinazinalone chemical group, based on a new mode of biological activity. It is reported that mode of action is modifies insect behavior, rapidly stopping feeding such that insects starve to death. Time-release feature and mortality effect on M. persicae using different pyrifluquinazon nano type and non-nano type were compared. Pyrifluquinazon nano type was formulated with different molecular weight and density of used chitosan (CS 30000 0.1% and CS 3000 0.3%). In the CS 30,000 0.1%, the mortality was weakly occurred at early time, but steadily increased after 4days. Finally, we confirmed more than 70% mortality as a peak at 16days. In CS 3000 0.3%, the mortality showed about 70% until 18days as a effective controlled release. Also, We examine time-release feature and mortality effect on M. persicae according to the different pyrifluquinazon nano type(CS 30000 0.1% and CS 3000 0.3%) of concentrations. The CS 30000 0.1% bioassay results of different concentration were showed that the highest concentration(100ppm) was measured better mortality than other concentration at 0 day, but cannot confirm different effect about dissimilar concentration. However, increasing rates of M. persicae were low as treatment concentrate was high. In CS 3000 0.3% 100ppm concentration bioassay result, aphid mortality reached peak at 24 days and increasing rate also low. Additionally, for the comparing of bioassay and feeding behavior of M. persicae against pyrifluquinazon nano types and non-nano type, EPG technique was carried out. In case of non nano type, feeding inhibition efficacy was showed during 4 days after treatment, but appeared similar level with control after 10days. In CS 3000 0.3% 50ppm, residual efficacy was specially showed until 28days after treatment whereas treatments with CS 30000 0.1% were similar to the control after 22days. These result show that the change of feedinng behavior and motrality of M. persicae is correlated with the change of nano type or non nano type of pyrifluquinazon.

HSPF 모형을 이용한 청미천 유역에서의 Chl-a 모의

강미리 ( Mi Ri Kang ),김덕우 ( Deok Woo Kim ),신창민 ( Chang Min Chin ) 한국물환경학회(구 한국수질보전학회) 2011 공동 추계학술발표회 Vol.2011 No.-

국내 청각장애 학습자를 위한 무용교육의 실태와 제언

강미리(Kang, Mi-Ri),박순자(Park, Soon-Ja) 학습자중심교과교육학회 2021 학습자중심교과교육연구 Vol.21 No.16

목적 본 연구에서는 청각장애인을 대상으로 그 실태를 밝히고 청각장애 학습자를 위한 특수학교 내 무용교육 현황에 대하여 제언하였다. 방법 이를 위하여 저서 및 논문, 보건복지부, 한국장애인고용공단, 한국보건사회연구원, 행정안전부, 국립재활원, 통계청 자료를 활용하였으며 국내 21개교의 청각장애 특수학교 홈페이지와 신문기사를 살펴봄으로써 수행되었다. 결과 청각장애 특수학교 내 무용교육의 실시를 분석한 결과 총 21개의 특수학교 중 방과후⋅자유활동 교육과정이 공개된 11개의 학교에서 6개의 학교에서만 실시하고 있었다. 무용은 비정규과정에 해당하는 수업으로 진행이 되고 있는데, 순위별로 장르를 보면 한국무용, 방송댄스, K-pop댄스와 라인댄스는 동일한 비중이며, 마지막으로 재즈댄스이다. 과거 무용교육의 방법이 음악수업 리듬교육의 일환으로 율동과 수화가 실시되었다면 순수무용의 한국무용과 대중적인 유행을 이끄는 방송댄스, K-pop댄스의 실용무용 위주로 점차 신체의 활동성이 확대되고 있다. 한국무용과 방송댄스는 초등부의 과정에서 수업이 진행되고 있고 K-pop댄스와 라인댄스는 초⋅중⋅고등부의 과정에서 진행됨을 알 수 있었다. 추후 더 많은 교육과정(중등부, 고등부)에 전문적인 무용교사와 교육의 확대가 필요하다. 결론 연구결과를 바탕으로 청각장애 학습자의 무용교육이 제고되어지고 이들을 위한 많은 연구가 요구된다. 따라서 청각장애인들을 위한 무용교육의 필요성을 시사하며, 연구의 활성화를 위하여 후속연구의 방향을 제시하였으며 향후 전문적인 특수무용교육을 위해 어떠한 노력과 지원이 필요한지에 대해 제안하였다. Objectives This study aimed to shed light on the reality of people with hearing impairments and make proposals for current dance education at special schools for students with hearing impairments. Methods For this purpose, the investigator used a range of data including books and papers and materials of Ministry of Health and Welfare, Korea Employment Agency for the Disabled, Korea Institute for Health and Social Affairs, Ministry of the Interior and Safety, National Rehabilitation Center and National Statistical Office, as well as the homepages of 21 special schools for people with hearing impairments in South Korea and newspaper articles. Results The study analyzed dance education at special schools for students with hearing impairments and found that dance education was provided at only six of 11 that made public their after-school and free activity curriculums among the 21 special schools. Dance lessons were given as part of a non-regular curriculum and covered Korean dance, broadcast dance, K-pop dance, and line dance equally with jazz dance covered least. Unlike the old approach of dance education involving dance routines and sign language as part of rhythm education in music lessons, these dance lessons gradually expanded the activity of the body toward Korean dance as pure dance and practical dance such as broadcast dance and K-pop dance leading popular vogues. While Korean and broadcast dance was covered in elementary school lessons, K-pop and line dance was covered in elementary, middle, and high school lessons. In the future, there will be a need to expand professional dance teachers and education to more special schools and curriculums. Conclusions Based on these findings, dance education should be boosted for students with hearing impairments with more researches to be conducted for them. These findings imply a need for dance education for people with hearing impairments and propose a direction for a follow-up study to vitalize researches on the subject. The study also made proposals regarding efforts and resources needed for professional special dance education in the future.

수중광량 관측을 통한 낙동강 본류 소광특성 연구

강미리 ( Mi-ri Kang ),최정규 ( Jungkyu Choi ),민중혁 ( Joong-hyuk Min ),신창민 ( Changmin Shin ),최환규 ( Hwangyu Choi ),박수영 ( Suyoung Park ),공동수 ( Dongsoo Kong ) 한국물환경학회 2017 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2017 No.-

수중 광량 모니터링을 통한 하절기 낙동강 본류 소광 특성 연구

강미리 ( Mi-ri Kang ),민중혁 ( Joong-hyuk Min ),최정규 ( Jungkyu Choi ),박수영 ( Suyoung Park ),신창민 ( Changmin Shin ),공동수 ( Dongsoo Kong ),김한순 ( Han Soon Kim ) 한국물환경학회(구 한국수질보전학회) 2018 한국물환경학회지 Vol.34 No.6

Algal dynamics is controlled by multiple environmental factors such as flow dynamics, water temperature, trophic level, and irradiance. Underwater irradiance penetrating from the atmosphere is exponentially decreased in water column due to absorption and scattering by water molecule and suspended particles including phytoplankton. As the exponential decrease in underwater irradiance affects algal photosynthesis, regulating their spatial distribution, it is critical to understand the light extinction characteristics to find out the mechanisms of algal dynamics more systematically. Despite the significance, the recent data have been rarely reported in the main stream areas of large rivers, Korea. In this study, the euphotic depths and light extinction coefficients were determined by monitoring the vertical variation of underwater irradiance and water quality in the main channel of Nakdong River near Dodong Seowon once a week during summer of 2016. The average values of euphotic depth and light extinction coefficient were 4.0 m and 1.3 m^-1, respectively. The degree of light extinction increased in turbid water due to flooding, causing an approximate 50 % decrease in euphotic depth. Also, the impact was greater than the self-shading effect during the periods of cyanobacterial bloom. The individual light extinction coefficients for background, total suspended solid and algal levels, frequently used in surface water quality modeling, were determined as 0.305 m^-1, 0.090 m^-1/mg·L^-1, 0.013 m^-1/μg·L^-1, respectively. The values estimated in this study were within or close to the ranges reported in literatures.

Different elution modes and field programming in gravitational field-flow fractionation : Effect of channel angle

Park, Mi Ri,Kang, Da Young,Chmelik, Josef,Kang, Namgoo,Kim, Jin Seog,Lee, Seungho Elsevier 2008 Journal of chromatography Vol.1209 No.1

<P><B>Abstract</B></P><P>Gravitational field-flow fractionation (GrFFF) has been shown to be useful for separation and characterization of various types of micrometer-sized particles. It has been recognized however that GrFFF is less versatile than other members of FFF because the external field (Earth's gravity) in GrFFF is relatively weak and is not tunable (constant), which makes the force acting on the particles constant. A few approaches have been suggested to control the force acting on particles in GrFFF. They include (1) changing the angle between the Earth's gravitational field and the longitudinal axis of the channel, and (2) the use of carrier liquid having different densities. In the hyperlayer mode of GrFFF, the hydrodynamic lift force (HLF) also act on particles. The existence of HLF allows other means of changing the force acting on the particles in GrFFF. They include (1) the flow rate programming, or (2) the use of channels having non-constant cross-section. In this study, with polystyrene latex beads used as model particles, the channel angle was varied to study its effect on elution parameters (such as selectivity, band broadening and resolution) in the steric or in the hyperlayer mode of GrFFF. In addition, the effects of the channel thickness and the flow rate on the elution parameters were also investigated. It was found that, in the steric mode, the resolution decreases as the flow rate increases due to increased zone broadening despite of the increase in the selectivity. At a constant volumetric flow rate, both the zone broadening and the selectivity increase as the channel thickness increases, resulting in the net increase in the resolution. It was also found that the retention time decreases as the channel angle increases in both up- and down-flow positions. The zone broadening tends to increase almost linearly with the channel angle, while no particular trends were found in selectivity. As a result, the resolution decreases as the channel angle increases.</P>

External Morphology and Distribution of Antennal Sensilla of Aphidius colemani

Hye Ri. Kwon,Min Ah Kang,Kyu Sik Yoon,Min Woo Park,Eun Jin Kang,Mi Ja Seo,Yong Man Yu,Young Nam Youn 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Several species of the genus Aphidius are used in biological control programs against aphid pests throughout the world and their behavior and physiology are well studied. But despite knowing the importance of sensory organs in their behavior, their antennal structure is largely unknown. In this study, the external morphology and distribution of the antennal sensilla on the antennal of both female and male adults of A. colemani were described using scanning electron microscopy (SEM). Generally, the filaform antennae of males (1,565.60± 194.64㎛) are longer than females (1,303.83±156.41㎛). Antennae of this species is made up of scape, pedicel and flagellomeres. Male and female antennae differed in the total number of flagellomeres as 15 in males and 13 in females. Female and male antennae of A. colemani has samely ten types of sensilla. We classified 3 types of sensilla trichodea as without pores, with a tip pore and with wall pores, 3 types of sensilla coeloconica, 1 sensilla placodea and 1 Bohm bristles. In addition, the possible functions of the above sensilla types are discussed in light of previously published literature; mechanoreception (Bohm bristles and sensilla coeloconica) and olfaction (sensilla trichodea and sensilla placodea). Future studies on the functional morphology of the antennal sensilla of A. colemani using transmission electron microscopy (TEM) coupled with electrophysiological recordings will likely confirm the functions of the different sensilla identified in this study.

HSPF 모형을 이용한 한강 유역에서의 수질예측

강미리 ( Mi Ri Kang ),이은정 ( Eun Jeong Lee ),최환규 ( Hwan Gyu Choi ),신창민 ( Chang Min Chin ) 한국물환경학회 ( 구 한국수질보전학회 ) 2012 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2012 No.-

Molecular Cloning of Prophenoloxidase Genes from the Multicolored Asian Lady Bird Beetle, Harmonia axyridis

Min Ah Kang,Kyu Sik Yoon,Hye Ri Kwon,Min Woo Park,Eun Jin Kang,Mi Ja Seo,Yong Man Yu,Young Nam Youn 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

The multicolored Asian ladybird beetle, Harmonia axyridis, is a generalist predator of aphids also, shows a high level of phenotype polymorphism in color pattern of elytra. Although, it is not sure about genetic information of color polymorphism, it has been confirmed that this phenomenon comes from their genetic traits. The color of H. axyridis elytra is mainly composed of black and red pigment. Phenoloxidase (PO) plays an important role in many insect physiological functions, i.e. sclerotization and pigmentation of cuticle and melanization of parasites. Following activation, PO catalyses the hydroxylation of tyrosine and subsequent oxidation of phenolic substance into quinines, which are further converted to melanin. However, the molecular bases of H. axyridis color pattern formation are almost unknown but it may be that the different pro-POs have different expression. In this study, total RNA samples from four each color pattern individuals, for example, succinea 1, succinea 2, conspicua and spectabilis was extracted. A cDNA enconding pro-PO was molecular cloned from each color pattern of H. axyridis and its putative amino acid sequence shared homology with pro-PO of other insects. We are pursuing to elucidate that their pro-PO sequence will be similar with those other insect PPO sequence. There are also regions of high sequence similarity, including putative activation site and two copper binding sites.