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Miguel Sogbe,Idoia Bilbao,Francesco P. Marchese,Jon Zazpe,Annarosaria De Vito,Marta Pozuelo,Delia D’Avola,Mercedes Iñarrairaegui,Carmen Berasain,Maria Arechederra,Josepmaria Argemi,Bruno Sangro 대한간학회 2024 Clinical and Molecular Hepatology(대한간학회지) Vol.30 No.2
Background/Aims: New prognostic markers are needed to identify patients with hepatocellular carcinoma (HCC) who carry a worse prognosis. Ultra-low-pass whole-genome sequencing (ULP-WGS) (≤0.5× coverage) of cell-free DNA (cfDNA) has emerged as a low-cost promising tool to assess both circulating tumor DNA (ctDNA) fraction and large structural genomic alterations. Here, we studied the performance of ULP-WGS of plasma cfDNA to infer prognosis in patients with HCC. Methods: Plasma samples were obtained from patients with HCC prior to surgery, locoregional or systemic therapy, and were analyzed by ULP-WGS of cfDNA to an average genome-wide fold coverage of 0.3x. ctDNA and copy number alterations (CNA) were estimated using the software package ichorCNA. Results: Samples were obtained from 73 HCC patients at different BCLC stages (BCLC 0/A: n=37, 50.7%; BCLC B/C: n=36, 49.3%). ctDNA was detected in 18 out of 31 patients who received systemic treatment. Patients with detectable ctDNA showed significantly worse overall survival (median, 13.96 months vs not reached). ctDNA remained an independent predictor of prognosis after adjustment by clinical-pathologic features and type of systemic treatment (hazard ratio 7.69; 95%, CI 2.09–28.27). Among ctDNA-positive patients under systemic treatments, the loss of large genomic regions in 5q and 16q arms was associated with worse prognosis after multivariate analysis. Conclusions: ULP-WGS of cfDNA provides clinically relevant information about the tumor biology. The presence of ctDNA and the loss of 5q and 16q arms in ctDNA-positive patients are independent predictors of worse prognosis in patients with advanced HCC receiving systemic therapy.
Dietary Fiber, Gut Peptides, and Adipocytokines
David Sa´nchez,Marta Miguel,Amaya Aleixandre 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.3
The consumption of dietary fiber (DF) has increased since it was related to the prevention of a range of illnesses and pathological conditions. DF can modify some gut hormones that regulate satiety and energy intake, thus also affecting lipid metabolism and energy expenditure. Among these gut hormones are ghrelin, glucagon-like peptide 1, peptide YY, and cholecystokinin. Adipose tissue is known to express and secrete a variety of products known as ‘‘adipocytokines,’’which are also affected by DF. Some of the most relevant adipocytokines include adiponectin, leptin, tumor necrosis factor-a,and interleukin-6. The release of adipocytokines, by either adipocytes or macrophage-infiltrated adipose tissue, leads to a chronic subinflammatory state that could play a central role in the development of insulin resistance and type 2 diabetes,therefore increasing the risk of cardiovascular disease associated with obesity. DF modulation of these molecules could also have positive effects on obesity, insulin resistance, and hyperlipidemia. This review is focused on the effects of DF on the above-mentioned gut peptides and adipocytokines.
Functionalization of 3D printed ABS filters with MOF for toxic gas removal
Ismael Pellejero,Fernando Almazán,Marta Lafuente,Miguel A. Urbiztondo,Martin Drobek,Mikhael Bechelany,Anne Julbe,Luis M. Gandía 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.89 No.-
Acrylonitrile butadiene styrene (ABS) is one of the most extensively used polymer in 3D printingmanufacturing due to its competitive thermal and mechanical properties. Recently, a special attentionhas been devoted to novel ABS composites featuring extra functionalities e.g. in the area of VOC removal. Herein, we report on a facile protocol for the functionalization of 3D printed ABSfilters with a MOF(Metal-Organic Framework) material (ZIF-8) targeting the conception of attractive gasfilters. Theproposed synthesis strategy consists in low temperature ALD (Atomic Layer Deposition) of ZnO on theABS grid followed by the hydrothermal conversion of ZnO to ZIF-8, both steps being conducted at 60 C. Insuch way, the method enables an effective growth of ZIF-8 without altering the stability of the polymericABS support. The as-fabricated ABS/ZIF-8filters offer a promising adsorption behaviour for dimethylmethylphosphonate ( 20.4 mg of DMMP per gram of ZIF-8), thus proving their potential for toxic gascapture applications.
Alicia Reyes Samilpa,Diana P. Ferreira,Marta A. Teixeira,Raul Fangueiro,Miguel C. Gutiérrez 한국섬유공학회 2022 Fibers and polymers Vol.23 No.3
In this study, lignocellulosic fibers from Agave salmiana –an important socio-economical species endemic toMexico– were used to reinforce thermoplastic maize starch (TPS). Fibers from young raw leaves (YRL) and old roastedleaves (ORL) were obtained according to the traditional methods used by small producers. The formulations of biocompositeswere obtained varying the content of both types of fiber and processed by extrusion and injection molding. Morphological,structural, mechanical, thermal, and thermomechanical properties of biocomposites were evaluated. To use the hydrophilicityof these materials as an advantage in unexplored applications, biocomposites behavior under degradative tests such asaccelerated aging and salt water immersion was evaluated. The processes of heating the old leaves partially removed thelignin and hemicellulose layer from the fibers, which led to a better interaction fiber-matrix, as confirmed by FESEM, ATRFTIR,and TGA. Biocomposites with 30 wt% of YRL fiber reported the highest values of tensile strength and Young’smodulus when compared to ORL biocomposites and with TPS. Accelerated aging exposure affected mainly thethermomechanical properties of TPS and confirmed the reinforcing effect of the fibers due to the thermal and mechanicalstability they provided to the matrix, especially when 20 wt% of fiber was added. This was also observed when biocompositeswere immersed in salt water solution. Using Agave salmiana fiber obtained from different leaf ages by traditional methods in theproduction of biocomposites promotes the complete harnessing of this species and represents a possibility to small producersin Mexico to introduce circular economy in their communities.
Electroconductive PEDOT nanoparticle integrated scaffolds for spinal cord tissue repair
Aleksandra Serafin,Mario Culebras Rubio,Marta Carsi,Pilar Ortiz-Serna,Maria J. Sanchis,Atul K. Garg,J. Miguel Oliveira,Jacob Koffler,Maurice N. Collins 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4
Background: Hostile environment around the lesion site following spinal cord injury (SCI) prevents the re-establishment of neuronal tracks, thus significantly limiting the regenerative capability. Electroconductive scaffolds are emerging as a promising option for SCI repair, though currently available conductive polymers such as polymer poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) present poor biofunctionality and biocompatibility, thus limiting their effective use in SCI tissue engineering (TE) treatment strategies. Methods: PEDOT NPs were synthesized via chemical oxidation polymerization in miniemulsion. The conductive PEDOT NPs were incorporated with gelatin and hyaluronic acid (HA) to create gel:HA:PEDOT-NPs scaffolds. Morphological analysis of both PEDOT NPs and scaffolds was conducted via SEM. Further characterisation ncluded dielectric constant and permittivity variances mapped against morphological changes after crosslinking, Young’s modulus, FTIR, DLS, swelling studies, rheology, in-vitro, and in-vivo biocompatibility studies were also conducted. Results: Incorporation of PEDOT NPs increased the conductivity of scaffolds to 8.3 × 10–4± 8.1 × 10–5 S/cm. The compressive modulus of the scaffold was tailored to match the native spinal cord at 1.2 ± 0.2 MPa, along with controlled porosity. Rheological studies of the hydrogel showed excellent 3D shear-thinning printing capabilities and shape fidelity post-printing. In-vitro studies showed the scaffolds are cytocompatible and an in-vivo assessment in a rat SCI lesion model shows glial fibrillary acidic protein (GFAP) upregulation not directly in contact with the lesion/implantation site, with diminished astrocyte reactivity. Decreased levels of macrophage and microglia reactivity at the implant site is also observed. This positively influences the re-establishment of signals and initiation of healing mechanisms. Observation of axon migration towards the scaffold can be attributed to immunomodulatory properties of HA in the scaffold caused by a controlled inflammatory response. HA limits astrocyte activation through its CD44 receptors and therefore limits scar formation. This allows for a superior axonal migration and growth towards the targeted implantation site through the provision of a stimulating microenvironment for regeneration. Conclusions: Based on these results, the incorporation of PEDOT NPs into Gel:HA biomaterial scaffolds enhances not only the conductive capabilities of the material, but also the provision of a healing environment around lesions in SCI. Hence, gel:HA:PEDOT-NPs scaffolds are a promising TE option for stimulating regeneration for SCI.
Pseudotumoral Presentation of Cerebral Amyloid-Beta Angiopathy: Case Report and Review of Literature
Claudia Uribe Roca,Fabio Maximiliano Gonzalez,Marta Ines Bala,Miguel Saucedo,Lucrecia Bandeo,Luciana Leon Cejas,Sol Pacha,Pablo Bonardo,Carlos Rugilo,Pablo Dezanzo,Rafael Torino,Gustavo Sevlever,Manue 대한신경정신의학회 2021 PSYCHIATRY INVESTIGATION Vol.18 No.6
Objective Cerebral amyloid angiopathy-related inflammation (CAA-RI) is a rare and potentially treatable encephalopathy that usually affects people older than 50 years old and has an acute or subacute clinical presentation characterized by rapidly evolving cognitive decline, focal deficits and seizures. In a small subset of patients the disease can adopt a pseudotumoral form in the neuroimages that represents a very difficult diagnostic challenge. Methods Here in we report a patient with a tumour-like presentation of histopathologically confirmed CAA-RI. Results We also conducted a search and reviewed the clinical and radiological features of 41 cases of pseudotumoral CAA-RI previously reported in the literature in order to identify those characteristics that should raise diagnostic suspicions of the disease, there by avoiding unnecessary surgical treatments.Conclusion The therapy of CAA-RI with steroids is usually effective and clinical and radiological remission can be achieved in the first month in approximately 70% of cases.
Francisco Caiza-Zambrano,Carolina Mora Palacio,Silvia Garbugino,Fabio Maximiliano Gonzalez,Marta Bala Biolcati,Miguel Ángel Saucedo,Carlos Rugilo,Mariano Forrester,Fernando Lombi,Manuel Fernández Pard 대한신경중재치료의학회 2022 Neurointervention Vol.17 No.1
Central venous disease (CVD) is a serious complication in hemodialysis patients. Neurological manifestations are rare. We describe a female with end-stage renal disease with throbbing headache accompanied by paresthesia, weakness, and abnormal posture of her right hand during dialysis sessions. Motor symptoms completely resolved after each dialysis session, although the headaches persisted for several hours. No neurological deficit was evidenced on physical examination. Digital subtraction angiography identified an incomplete thrombosis of the left brachiocephalic vein with retrograde flow in the internal jugular vein, sigmoid sinus, and transverse sinus on the left side. This case illustrates that cerebral venous congestion due to CVD can produce neurological symptoms. Furthermore, we systematically review the literature to identify the characteristics of the cases described so far. This allows clinicians to know the entity and have a high index of suspicion in a hemodialysis patient who develops neurological symptoms.
Marcela S ˇ afratova,Anna Hosˇt’a´lkova,Daniela Hulcova,Katerˇina Breiterova,Veronika Hrabcova,Marta Machado,Diana Fontinha,Miguel Prudeˆncio,Jirˇı´ Kunes,Jakub Chlebek,Daniel Jun,Martina Hrabinova,Lu 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.2
Fifteen Amaryllidaceae alkaloids (1–15) of variousstructural types were isolated by standard chromatographicmethods from fresh bulbs of Narcissus poeticus cv. Pink Parasol. The chemical structures were elucidated byMS, and 1D and 2D NMR spectroscopic analyses, and bycomparison with literature data. Narcipavline (5) and narcikachnine(6) are reported here for the first time. In theirstructure are combined two basic structural types ofAmaryllidaceae alkaloids (galanthamine- and galanthindole-structural types), which represent a new structuraltype of these compounds. Alkaloids isolated in sufficientamounts were evaluated for their human erythrocyticacetylcholinesterase, and human serum butyrylcholinesterase(HuBuChE) inhibition activity using Ellman’smethod. Z-Gly-Pro-p-nitroanilide was used assubstrate in the prolyl oligopeptidase (POP) assay. Untestedalkaloids were also screened for their cytotoxic activityagainst a small panel of human cancer cells, which spannedcell lines from different tissue types. In parallel, MRC-5human fibroblasts were employed to determine overalltoxicity against noncancerous cells. Some compounds wereevaluated for their antiprotozoal activity. The newly isolatedalkaloid narcipavline (5) showed interestingHuBuChE inhibition activity (IC50 = 24.4 ± 1.2 lM), andnorlycoramine (11) demonstrated promising POP inhibition(IC50 = 0.21 ± 0.01 mM).