Binding of erucic acid with human serum albumin using a spectroscopic and molecular docking study

Rabbani, G.,Baig, M.H.,Jan, A.T.,Ju Lee, E.,Khan, M.V.,Zaman, M.,Farouk, A.E.,Khan, R.H.,Choi, I. Elsevier 2017 International journal of biological macromolecules Vol.105 No.3

Erucic acid (EA) is one of the key fatty acids usually found in canola oil, mustard oil and rapeseed oil. Consumption of EA in primates was found to cause myocardial lipidosis and cardiac steatosis. To have an insight of the effect of EA in humans, we performed in vitro interaction studies of EA with the primary plasma protein, human serum albumin (HSA). Spectroscopic (UV-vis and fluorescence) analysis of the HSA-EA interaction revealed a static mode of quenching with binding constant K<SUB>b</SUB> ~10<SUP>4</SUP> reflecting high affinity of EA for HSA. The negative value of ΔG<SUP>o</SUP> for binding of EA to HSA in the fluorescence studies indicates the process to be spontaneous. Thermodynamic signatures of the HSA-EA interaction in the complex reflect dominance of hydrogen bonds. Despite predominance of hydrogen bonds, hydrophobic interactions in the HSA-EA complex were found acting as a contributing factor in the binding of EA to HSA, observed as structural change in the far-UV CD spectra. Forster's resonance energy transfer of the EA-HSA complex revealed a distance of 3.2nm between acceptor molecules (EA) and the donor Trp residue of HSA. To have a deeper insight of the structural dependence of the HSA-EA interaction in the complex, thermodynamic study was supplemented with molecular docking. The molecular docking analysis further highlighted the EA binding in the subdomain IIIA (Sudlow site II) of HSA. The information generated in the study reflects greater pharmacological significance of EA and highlights its importance in the clinical medicine.

Bi2InTaO7 compounds as promising photocatalysts for marine plankton removal

Leticia M. Torres-Martínez,Isaías Juárez-Ramírez,Juan S. Ramos-Garza,Francisco Vázquez-Acosta,Ricardo Gómez,ZhengYi Fu,Soo Wohn Lee 한양대학교 세라믹연구소 2011 Journal of Ceramic Processing Research Vol.12 No.1

The removal of marine plankton, red (Amphidium carterae), and green (Tetraselmis suecica) tide algae, by heterogeneous photocatalysis was carried out using pyrochlore-type semiconductors. The semiconductor, Bi2InTaO7 , was prepared by the solgel method and a solid state reaction. The XRD patterns evidence formation of the pyrochlore phase in the sol-gel materials calcined at low temperatures (600-800 oC). Nanostructured sol-gel materials were observed by SEM. The sol-gel preparation allowed the formation of the pyrochlore-type compound with specific surface areas (7-13 m2g−1) higher than the solid obtained by solid state preparation (< 5 m2g−1). Band gap (Eg) values between 2.1-2.7 eV determined by UV-Vis spectroscopy were obtained. Bi2InTaO7 photocatalysts prepared by the sol-gel method were able to eliminate Tetraselmis suecica and Amphidium carterae algae totally after 50 minutes of UV-irradiation, whereas Bi2InTaO7 by solid state preparation needed more than 1 h.

Effects of Organic or Inorganic Acid Supplementation on Growth Performance, Nutrient Digestibility and White Blood Cell Counts in Weanling Pigs

D. Y. Kil,L. G. Piao,H. F. Long,J. S. Lim,M. S. Yun,C. S. Kong,W. S. Ju,H. B. Lee,Y. Y. Kim 아세아·태평양축산학회 2006 Animal Bioscience Vol.19 No.2

Four experiments were conducted to investigate the effect of organic or inorganic acid supplementation on the growth performance, nutrient digestibility, intestinal measurements and white blood cell counts of weanling pigs. In growth trial (Exp I), a total of 100 crossbred pigs ({Landrace횞Yorkshire}횞Duroc), weaned at 23짹2 days of age and 7.25짹0.10 kg average initial body weight (BW), were allotted to 5 treatments by body weight and sex in a randomized complete block (RCB) design. Three different organic acids (fumaric [FUA], formic [FOA] or lactic acid [LAA]) and one inorganic acid (hydrochloric acid [SHA]) were supplemented to each treatment diet. Each treatment had 5 replicates with 4 pigs per pen. During 0-3 wk, average daily gain (ADG), average daily feed intake (ADFI) and feed efficiency (G/F ratio) were not significantly different among treatments. However, pigs fed LAA or SHA diet showed improved ADG by 15 or 13% respectively and 12% greater ADFI in both treatments compared to CON diets. Moreover, compared to organic acid treatments, better ADG (p = 0.07) and ADFI (p = 0.09) were observed in SHA diet compared to pigs that were fed the diet containing organic acids (FUA, FOA or LAA). However, during 4-5 wk, no differences in ADG, ADFI and G/F ratio were observed among treatments. Overall, ADG, ADFI and G/F ratio were not affected by acidifier supplementation. Although it showed no significant difference, pigs fed LAA or SHA diets showed numerically higher ADG and ADFI than pigs fed other treatments. In metabolic trial (Exp II), 15 pigs were used to evaluate the effect of acidifier supplementation on nutrient digestibility. The digestibility of dry matter (DM), crude protein (CP), crude fat (CF), crude ash (CA), calcium (Ca) and phosphorus (P) was not improved by acidifier supplementation. Although the amount of fecal-N excretion was not different among treatments, that of urinary-N excretion was reduced in acidsupplemented treatments compared to CON group (p = 0.12). Subsequently, N retention was improved in acid-supplemented groups (p = 0.17). In anatomical trial (Exp III), the pH and Cl- concentrations of digesta in gastrointestinal (GI) tracts were not affected by acidifier supplementation. No detrimental effect of intestinal and lingual (taste bud) morphology was observed by acidifier supplementation particularly in inorganic acid treatment. In white blood cell assay (Exp IV), 45 pigs were used for measuring white blood cell (WBC) counts. In all pigs after LPS injection, WBC counts had slightly declined at 2 h and kept elevating at 8 h, then returned to baseline by 24 h after injection of lipopolysaccharide (LPS). However, overall WBC counts were not affected by acidifier supplementation. In conclusion, there was no difference between organic and inorganic acidifier supplementation in weanling pigs' diet, however inorganic acidifier might have a beneficial effect on growth performance and N utilization with lower supplementation levels. Furthermore, inorganic acidifier had no negative effect on intestinal measurements and white blood cell counts in weanling pigs. These results suggested that inorganic acidifier might be a good alternative to organic acidifiers in weanling pigs.

Evaluation of Ni Film Interfacial Energy Release Rate on Titanium and Stainless Steel Substrates

Ren, F. Z.,Zhao, W. Z.,Zhou, G. S.,Ju, X. H.,Zheng, M. 대한금속학회 2002 METALS AND MATERIALS International Vol.8 No.2

An expression including the effect of residual stress on the interfacial energy release rate is proposed for peeling experiments according to the energy-balance argument. The influence of residual stress on the external work is also contained in the expression. Two numerical methods are employed to evaluate the values of the work expenditure G_db, which is the actual energy dissipated during bending of the peel arm near the peel front. The peeling method is employed to test the interfacial energy release rates, G, for Ni films on Titanium and stainless steel substrates. The results indicate that the value of G for Ni films on stainless steel substrate is about 5.47-6.03 N/m, while 5.23-6.71 N/m for Ni films on titanium substrate; the interfacial energy release rates, G, do not depend on the residual stress in film, film thickness nor peel angle. The effect of residual stress in film on peel strength P/h is also discussed.

Insulating phases of vanadium dioxide are Mott-Hubbard insulators

Huffman, T. J.,Hendriks, C.,Walter, E. J.,Yoon, Joonseok,Ju, Honglyoul,Smith, R.,Carr, G. L.,Krakauer, H.,Qazilbash, M. M. American Physical Society 2017 Physical Review B Vol.95 No.7

<P>We present comprehensive broadband optical spectroscopy data on two insulating phases of vanadium dioxide (VO2): monoclinic M-2 and triclinic. The main result of our work is that the energy gap and the electronic structure are essentially unaltered by the first-order structural phase transition between the M-2 and triclinic phases. Moreover, the optical interband features in the M-2 and triclinic phases are remarkably similar to those observed in the well-studied monoclinic M-1 insulating phase of VO2. As the energy gap is insensitive to the different lattice structures of the three insulating phases, we rule out vanadium-vanadium pairing (the Peierls component) as the dominant contributor to the opening of the gap. Rather, the energy gap arises primarily from intra-atomic Coulomb correlations.</P>

INTERACTIONS OF THE INFRARED BUBBLE N4 WITH ITS SURROUNDINGS

Liu, Hong-Li,Li, Jin-Zeng,Wu, Yuefang,Yuan, Jing-Hua,Liu, Tie,Dubner, G.,Paron, S.,Ortega, M. E.,Molinari, Sergio,Huang, Maohai,Zavagno, Annie,Samal, Manash R.,Huang, Ya-Fang,Zhang, Si-Ju American Astronomical Society 2016 The Astrophysical journal Vol.818 No.1

<P>The physical mechanisms that induce the transformation of a certain mass of gas in new stars are far from being well understood. Infrared bubbles associated with H II regions have been considered to be good samples for investigating triggered star formation. In this paper we report on the investigation of the dust properties of the infrared bubble N4 around the H II. region G11.898+0.747, analyzing its interaction with its surroundings and star formation histories therein, with the aim of determining the possibility of star formation triggered by the expansion of the bubble. Using Herschel PACS and SPIRE images with a wide wavelength coverage, we reveal the dust properties over the entire bubble. Meanwhile, we are able to identify six dust clumps surrounding the bubble, with a mean size of 0.50 pc, temperature of about 22 K, mean column density of 1.7 x 10(22) cm(-2), mean volume density of about 4.4 x 10(4) cm(-3), and a mean mass of 320M(circle dot). In addition, from PAH emission seen at 8 mu m, free-free emission detected at 20 cm, and a probability density function in special regions, we could identify clear signatures of the influence of the H II region on the surroundings. There are hints of star formation, though further investigation is required to demonstrate that N4 is the triggering source.</P>

초음파 가진을 이용한 2-유체 노즐의 효율 향상

주은선,나우정,정진도,송민근,이경열 한국농업기계학회 2002 바이오시스템공학 Vol.27 No.4

초음파에 의한 액체 미립화 입경 분포도에 관한 연구

최우창,주은선,송민근,박영호 慶尙大學校生産技術硏究所 1998 生産技術硏究所論文集 Vol.14 No.-

A Study on size distributions of the liquid atomization by ultrasonic was carried out using 3 methods, such as direct vibration, aerosol and improvement method. Distilled water and city water were selected as the reference liquids and gasoline, kerosene and petroleum as fuel liquids. Two kinds of ultrasonic transducers, with 28kHz and 2MHz were used. Characteristics and phenomena on ultrasonic atomization were observed and examined relatively according to influx and liquid load by using the Malvern system. In results, The best uniformity of particle size was achieved by the direct vibration method, and their mean size was 10 times larger than that of the aerosol method.

초음파 적용 상온연무기용 2-유체 노즐의 분무입경 측정

나우정,주은선,정진도,송민근,이경열 경상대학교 농업생명과학연구원 2001 농업생명과학연구 Vol.35 No.-

상온연무기의 연무입자의 미립화에 대한 초음파 에너지의 효과를 구명하기 위하여 상온 연무기용 2-유체 노즐에 초음파 비가진 시의 상용분무, 분무노즐에 초음파 진동을 기계적 진동으로 주는 간접진동분무, 초음파를 분무액체에 직접 가하는 개질분무, 간접진동분무와 개질분무를 동시에 가하는 간접진동 및 개질법 겸용분무에 대하여 입경을 측정하였다. 분무방향 축과 반경방향 전 영역에 걸쳐서 사우터 평균지름(Sauter's mean diameter)을 측정한 결과 초음파 비가진 시인 상용분무시의 입경이 가장 크게, 간접진동 및 개질법 겸용의 경우가 가장 작게 나타났다. To figure out the effect of ultrasonic energy on the atomization of spray droplets from a twin-fluid nozzle used for cold-fog machine, spray droplets were measured according to the four experimental conditions; conventional spray where no ultrasonic energy is added, indirect vibration method where ultra vibration is added to the body of nozzle, liquid-property-change method where ultrasonic energy is added directly into the spray liquid, and combined addition method where indirect vibration method and liquid-property-change method are applied simultaneously. For all the regions according to the distance from the nozzle tip and for all radial regions, the poorest atomization was observed for the conventional spray condition and the best atomization was observed for the combined method conditions.

Room Temperature in Situ Growth of B/BO_<i>x</i> Nanowires and BO_<i>x</i> Nanotubes

Gonzalez-Martinez, Ignacio G.,Gorantla, Sandeep M.,Bachmatiuk, Alicja,Bezugly, Viktor,Zhao, Jiong,Gemming, Thomas,Kunstmann, Jens,Eckert, Jü,rgen,Cuniberti, Gianaurelio,Rü,mmeli, Mark H. American Chemical Society 2014 NANO LETTERS Vol.14 No.2

<P>Despite significant advances in the synthesis of nanostructures, our understanding of the growth mechanisms of nanowires and nanotubes grown from catalyst particles remains limited. In this study we demonstrate a straightforward route to grow coaxial amorphous B/BO<SUB><I>x</I></SUB> nanowires and BO<SUB><I>x</I></SUB> nanotubes using gold catalyst particles inside a transmission electron microscope at room temperature without the need of any specialized or expensive accessories. Exceedingly high growth rates (over 7 μm/min) are found for the coaxial nanowires, and this is attributed to the highly efficient diffusion of B species along the surface of a nanowire by electrostatic repulsion. On the other hand the O species are shown to be relevant to activate the gold catalysts, and this can occur through volatile O species. The technique could be further developed to study the growth of other nanostructures and holds promise for the room temperature growth of nanostructures as a whole.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2014/nalefd.2014.14.issue-2/nl404147r/production/images/medium/nl-2013-04147r_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl404147r'>ACS Electronic Supporting Info</A></P>