Adenovirus-mediated Double Suicide Gene Selectively Kills Gastric Cancer Cells

Luo, Xian-Run,Li, Jian-Sheng,Niu, Ying,Miao, Li Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

The aim of this study was to evaluate the effect of the adenovirus-mediated double suicide gene (CD/TK) for selective killing of gastric cancer cells. Gastric cancer cells SCG7901 and normal gastric epithelial cell lines were infected by adenoviruses Ad-survivin/GFP and Ad-survivin/CD/TK. GFP expression and CD-TK were detected by fluorescence microscopy and reverse transcriptase polymerase chain reaction (RT-PCR), respectively. After treatment of the infected cells with the pro-drugs ganciclovir (GCV) and/or 5-FC, the cell growth status was evaluated by methyl thiazolyl tetrazolium assay. Cell cycle changes were detected using flow cytometry. In nude mice bearing human gastric cancer, the recombinant adenovirus vector was injected directly into the tumor followed by an intraperitoneal injection of GCV and/or 5-FC. The subsequent tumor growth was then observed. The GFP gene driven by survivin could be expressed within the gastric cancer line SCG7901, but not in normal gastric epithelial cells. RT-PCR demonstrated the presence of the CD/TK gene product in the infected SCG7901 cells, but not in the infected normal gastric epithelial cells. The infected gastric cancer SCG7901, but not the gastric cells, was highly sensitive to the pro-drugs. The CD/TK fusion gene system showed significantly greater efficiency than either of the single suicide genes in killing the target cells (P<0.01). Treatment of the infected cells with the pro-drugs resulted in increased cell percentage in G0-Gl phase and decreased percentage in S phase. In nude mice bearing SCG7901 cells, treatment with the double suicide gene system significantly inhibited tumor growth, showing much stronger effects than either of the single suicide genes (P<0.01). The adenovirus-mediated CD/TK double suicide gene driven by survivin promoter combined with GCV an 5-FC treatment could be an effective therapy against experimental gastric cancer with much greater efficacy than the single suicide gene CD/TK combined with GCV or 5-FC.

LncRNA TMPO-AS1 promotes esophageal squamous cell carcinoma progression by forming biomolecular condensates with FUS and p300 to regulate TMPO transcription

Luo Xiao-Jing,He Ming-Ming,Liu Jia,Zheng Jia-Bo,Wu Qi-Nian,Chen Yan-Xing,Meng Qi,Luo Kong-Jia,Chen Dong-Liang,Xu Rui-Hua,Zeng Zhao-Lei,Liu Ze-Xian,Luo Hui-Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Esophageal squamous cell carcinoma (ESCC) is one of the most life- and health-threatening malignant diseases worldwide, especially in China. Long noncoding RNAs (lncRNAs) have emerged as important regulators of tumorigenesis and tumor progression. However, the roles and mechanisms of lncRNAs in ESCC require further exploration. Here, in combination with a small interfering RNA (siRNA) library targeting specific lncRNAs, we performed MTS and Transwell assays to screen functional lncRNAs that were overexpressed in ESCC. TMPO-AS1 expression was significantly upregulated in ESCC tumor samples, with higher TMPOAS1 expression positively correlated with shorter overall survival times. In vitro and in vivo functional experiments revealed that TMPO-AS1 promotes the proliferation and metastasis of ESCC cells. Mechanistically, TMPO-AS1 bound to fused in sarcoma (FUS) and recruited p300 to the TMPO promoter, forming biomolecular condensates in situ to activate TMPO transcription in cis by increasing the acetylation of histone H3 lysine 27 (H3K27ac). Targeting TMPO-AS1 led to impaired ESCC tumor growth in a patient-derived xenograft (PDX) model. We found that TMPO-AS1 is required for cell proliferation and metastasis in ESCC by promoting the expression of TMPO, and both TMPO-AS1 and TMPO might be potential biomarkers and therapeutic targets in ESCC.

Mechanical Prophylaxis Compared with Low-molecular-weight Heparins for Preventing Venous Thrombosis after Orthopedic Surgery: A Systematic and Meta-analysis

Xing-xian Luo,Jun-ping Han,Muhammad Usman,Charles D. Sands,Changqing Yang 한국병원약사회 2018 병원약사회지 Vol.35 No.3

Background : Mechanical prophylaxis (MP) and low-molecular-weight heparins (LMWHs) have been widely used after patient undergoing orthopedic surgery. However, their efficacy in preventing venous thrombus remains unclear. Methods : PubMed, Embase and Cochrane library databases were systematically searched for studies that investigated the effectiveness between MP and LMWHs. Predefined outcomes were incidence of deep vein thrombosis (DVT), pulmonary embolism (PE), proximal DVT and major bleeding using random-effects models. Results : A total of 8 randomized controlled trials (RCTs) on 2899 patients were eligible for inclusion. No significant difference was observed between MP and LMWHs for the prevention of DVT (RR 1.37; 95% CI: 0.83-2.26), PE (RR 1.35; 95% CI: 0.41-4.41), or proximal DVT (RR 1.30; 95% CI: 0.55- 3.02). However, there was a significant reduction of major bleeding (RR 0.21; 95% CI: 0.05-0.82). Predefined subgroup analysis suggested that MP might enhance the occurrence of DVT in comparison with fixed-dose LMWHs (RR 1.61; 95% CI: 1.09-2.37), but not in the subgroup with adjusted-weight LMWHs (RR 0.38; 95% CI, 0.11-1.30). Without combining graduated compression stockings (GCS) in both groups, the incidence of DVT was significantly associated with MP (RR 1.88; 95% CI: 1.01-3.21). Conclusions : Results of this meta-analysis suggested, compared to LMWHs, MP might have no significance in the prevention of DVT, although it was associated with lower incidence of major bleeding after patients underwent orthopedic surgery. However, subgroup analyses suggested that fixed-based LMWHs or application one of mechanical types without GCS might have differential effects. Therefore, large-scaled and well-designed RCTs are needed in the future.

A Novel Selenium- and Copper-Containing Peptide with Both Superoxide Dismutase and Glutathione Peroxidase Activities

Xian Feng Zou,Yue Tong Ji,Gui Gao,Xue Jun Zhu,Shao Wu Lv,Fei Yan,Si Ping Han,Xing Chen,Chang Cheng Gao,Jun Qiu Liu,Gui Min Luo 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.1

A potential industrialized fiber-supported copper catalyst for one-pot multicomponent CuAAC reactions in water

Xian-Lei Shi,Yongju Chen,Qianqian Hu,Wenqin Zhang,Chenxu Luo,Peigao Duan 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.53 No.-

A potential industrialized fiber catalyst for “click chemistry” via the one-pot multicomponent Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) for the synthesis of 1,2,3-triazoles in water, is reported. Detailed characterization by appearance, mechanical properties, elemental analysis, FTIR spectroscopy, and SEM confirmed the rangeability of the fiber catalyst during the preparation and utilization processes. Moreover, the fiber catalyst-mediated reactions proceeded smoothly to afford triazoles with nearly quantitative yields in short time (15 min). Furthermore, the fiber catalyst has shown tandem activities and superior recyclability (over 10 cycles), and the procedure is operationally simple and amenable to the gram-scale on a simple fixed-bed reactor.

Effects of bmim[PF6] treatments with different concentrations on microbial activity of Saccharomyces cerevisiae

Xian-Liang Song,Sheng-Ying Ye,Ru Xie,Ling Yin,Xun Shi,Shu-Can Luo 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.9

−To study the role of ionic liquid as a solvent in whole cell biocatalyst, it is necessary to probe the effects of ionic liquid treatments on microbial activity. In this paper, Saccharomyces cerevisiae was selected as a test bacterium to study the effects of bmim[PF6] ionic liquid in different concentration on yeast activity through determining the growth curve, sugar degradation curve, microbial activity, colonial morphology and cell morphology. The results revealed that the growth of yeast is inhibited strongly in low concentration ionic liquid, while the lethal effect is feeble in high concentration ionic liquid. This result was totally different from that of supercritical CO_2 treatment or high concentration benzene methanol treatment which would lead most yeast to death.

A Novel Two-Step Tensegrity Topology-Finding Method Based on Mixed Integer Programming and Nonlinear Programming

Xian Xu,Shaoxiong Huang,Tingting Shu,Yafeng Wang,Yao-Zhi Luo 한국강구조학회 2022 International Journal of Steel Structures Vol.22 No.4

A two-step topology-fi nding method based on mixed integer programming and nonlinear programming is proposed for tensegrity structures. In the fi rst step, feasible and symmetric strut connectivities are obtained through a ground structure method combined with mixed integer programming; in the second step, the cable connectivities are optimized through nonlinear programming to obtain a feasible tensegrity structure. The same ground structure used in the fi rst step is adopted in the second step, which is benefi cial to fi nd a more symmetric cable layout. The independent continuous mapping method is used in the second step to convert the 0–1 binary variables of cable connectivities to continuous variables to transform the combinatorial optimization problem into a nonlinear programming problem. The number of strut lengths is adopted as a control parameter and a symmetry objective function is proposed to generate a variety of regular and symmetric tensegrity structures. A multi-stage computation scheme is proposed to improve the computational effi ciency. Typical examples are carried out to validate the proposed method. The computational effi ciency of the method is benchmarked with existing methods fully based on mixed integer programming. Results demonstrate that the computational effi ciency of the proposed method is signifi cantly improved compared to the existing methods.

Particulate Matter 2.5 Causes Deficiency in Barrier Integrity in Human Nasal Epithelial Cells

Mu Xian,Siyuan Ma,Kuiji Wang,Hongfei Lou,Yang Wang,Luo Zhang,Chengshuo Wang,Cezmi A. Akdis 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.1

Purpose: The effect of air pollution-related particulate matter (PM) on epithelial barrier function and tight junction (TJ) expression in human nasal mucosa has not been studied to date. This study therefore aimed to assess the direct impact of PM with an aerodynamic diameter less than 2.5 μm (PM2.5) on the barrier function and TJ molecular expression of human nasal epithelial cells. Methods: Air-liquid interface cultures were established with epithelial cells derived from noninflammatory nasal mucosal tissue collected from patients undergoing paranasal sinus surgery. Confluent cultures were exposed to 50 or 100 μg/mL PM2.5 for up to 72 hours, and assessed for 1) epithelial barrier integrity as measured by transepithelial resistance (TER) and permeability of fluorescein isothiocyanate (FITC) 4 kDa; 2) expression of TJs using real-time quantitative polymerase chain reaction and immunofluorescence staining, and 3) proinflammatory cytokines by luminometric bead array or enzyme-linked immunosorbent assay. Results: Compared to control medium, 50 and/or 100 μg/mL PM2.5-treatment 1) significantly decreased TER and increased FITC permeability, which could not be restored by budesonide pretreatment; 2) significantly decreased the expression of claudin-1 messenger RNA, claudin-1, occludin and ZO-1 protein; and 3) significantly increased production of the cytokines interleukin-8, TIMP metallopeptidase inhibitor 1 and thymic stromal lymphopoietin. Conclusions: Exposure to PM2.5 may lead to loss of barrier function in human nasal epithelium through decreased expression of TJ proteins and increased release of proinflammatory cytokines. These results suggest an important mechanism of susceptibility to rhinitis and rhinosinusitis in highly PM2.5-polluted areas.

Experimental Investigation of R(ω), T(ω) and L(ω) for Multi-Layer SRRs and Wires Metamaterials

Hao Luo,Xian Wang,Zhangqi Liao,Tao Wang,Rongzhou Gong 한국전자파학회JEES 2010 Journal of Electromagnetic Engineering and Science Vol.10 No.3

Reflection(R(ω)), transmission(T(ω)) and loss(L(ω)) characteristics of multi-layer metamaterials are investigated experimentally in free space with the incident EM waves perpendicular to the substrate plane. The sample is made of split-ring resonators(SRRs) and wires which are the typical model of metamaterials. The R(ω) and T(ω) of multi-layer metamaterials have been calculated from the measured S-parameters. In this paper, we got the impedancematched result according to the curves of R(ω), meanwhile the T(ω) decreased with increasing number of layers. At last, we attained the result that the L(ω) gets to nearly 98% around 8 ㎓, with R(ω)=T(ω)=0. The design presented in this paper achieves experimented loss near unity.

Short-term Haze Exposure Predisposes Healthy Volunteers to Nasal Inflammation

Mu Xian,Kuiji Wang,Hongfei Lou,Yang Wang,Luo Zhang,Chengshuo Wang 대한천식알레르기학회 2019 Allergy, Asthma & Immunology Research Vol.11 No.5

Purpose: This study aimed to investigate the impact of short-term haze exposure on nasal inflammation in healthy volunteers. Methods: Thirty-three healthy university students were assessed for nasal symptoms, nasal patency, upper and lower respiratory tract nitric oxide (NO) as well as inflammatory mediators and neuropeptides in nasal secretions before and after a 5-day haze episode. Peripheral blood mononuclear cells (PBMCs) were stimulated with particulate matter with an aerodynamic diameter of less than 2.5 μm (PM2.5), and cytokines in the supernatants were examined. Results: Mild nasal symptoms were reported by some participants during the haze episode. Objective measures of nasal patency demonstrated that nasal airway resistance was significantly increased from baseline levels, while nasal cavity volume and minimum cross-sectional area were significantly decreased. Similarly, the levels of nasal and exhaled NO, eotaxin, interleukin (IL)-5, chemokine (C-C motif) ligand 17, IL-8, substance P, nerve growth factor and vasoactive intestinal peptides in nasal secretions were significantly increased from baseline values following the haze episode. In contrast, the levels of interferon-γ, IL-10, transforming growth factor-β and neuropeptide Y were significantly decreased. Incubation with 0.1-10 μg/mL PM2.5 significantly increased release of IL-1β, IL-4, IL-5, IL-8 and IL-10 from PBMCs. Conclusions: Short-term haze exposure may lead to nasal inflammation and hypersensitivity in healthy subjects predominantly by Th2 cytokine-mediated immune responses.