Down‑regulation expression of TGFB2‑AS1 inhibits the proliferation, migration, invasion and induces apoptosis in HepG2 cells

Wenrong Liu,Ruiping Huai,Yin Zhang,Shuquan Rao,Lili xiong,Ruofan Ding,Canquan Mao,Wenqing Zhao,Tao Hao,Qingqing Huang,Zhiyun Guo 한국유전학회 2019 Genes & Genomics Vol.41 No.8

Background Hepatocellular carcinoma (HCC) is the leading cause of cancer mortality and without effective prognosis. Previous study has been confirmed that the abnormal expression of long non-coding RNAs (lncRNAs) TGFB2-AS1 was involved in tumorigenesis. However, the biological functions of TGFB2-AS1 in hepatocellular carcinoma (HCC) remain largely unclear. Objective We comprehensively assess the clinical significance of TGFB2-AS1 and investigate the biological functions of TGFB2-AS1 on HCC HepG2 cells. Methods We firstly confirmed the expression of TGFB2-AS1 between tumor and normal tissues using public available transcriptome data. We analyzed the clinical significance of TGFB2-AS1 using the TCGA HCC datasets. The biological functions of TGFB2-AS1 on HCC HepG2 cells were explored by multiple in vitro assays. Results We found that TGFB2-AS1 was remarkably increased in HCC tissues (P = 0.00148) and exhibited a potential predictive marker for HCC, with an area under curve (AUC) of 0.708 (P = 0.0034) using the fifty pairs of matched HCC tissues of TCGA. Besides, higher expression of TGFB2-AS1 in HCC tissues was identified as being positively associated with advanced tumor (P = 0.012) and disease stage (P = 0.009) in 355 HCC cases using independent sample nonparametric test. Downregulation of TGFB2-AS1 expression significantly restrained proliferation (P < 0.01) and impaired colony formation (P < 0.05). Furthermore, TGFB2-AS1 depletion remarkably promoted the apoptosis of HepG2 cells (P < 0.05) and inhibited migration and invasion (P < 0.01). Conclusion Taken together, these findings suggested that TGFB2-AS1 might serve as a potential therapeutic target for HCC.

Effects of triflumezopyrim on the reproduction of brown planthopper, Nilaparvata lugens

Li Yao,Liu Jianqi,Sun Wenqing,Liu Fang 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

The brown planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae), is a notorious pest on rice in Asia and has evolved serious resistance to traditional chemical insecticides in recent years. Triflumezopyrim, a newly commercialized mesoionic insecticide, exhibited an excellent control effect on sucking insects, including N. lugens. However, the effects of triflumezopyrim on the reproduction of N. lugens have not yet been fully elucidated. In this study, we investigated the impacts of different concentrations of triflumezopyrim (LC 10 , LC 50 and LC 90 ) on the reproduction and the reproduction related genes of N. lugens exposure to the insecticide via systemic and contact route. The results indicated that the preoviposition periods of N. lugens females in the LC 50 and LC 90 triflumezopyrim-treated groups (♀ t × ♂ t) were prolonged in contact toxicity treatment, compared with control (acetone treatment). The fecundities for three pair combinations (♀ c ×♂ t, ♀ t ×♂ c and ♀ t ×♂ t) treated with LC 50 and LC 90 concentrations of triflumezopyrim showed a significant decrease compared to water/acetonetreated control via contact and systemic route. No significant effects of LC 10 triflumezopyrim on preoviposition period and fecundity were found in contact and systemic toxicity treatments. However, the triflumezopyrim did not influence the egg hatchability. By microscope observation, the LC 50 and LC 90 triflumezopyrim impended the ovarian development in females. qRT-PCR analysis showed that the vitellogenin (NlVg) relative expression level of female adults exposed to LC 50 and LC 90 triflumezopyrim decreased by 30.3 – 39.5% in two toxicity treatments. The titers of another reproduction related hormones juvenile hormone III (JH III) in LC 50 and LC 90 triflumezopyrim-treatments were 27.4% and 25.8% lower than control in contact toxicity treatment and 27.08% and 37.18% lower than control in systemic toxicity treatment. In summary, these results suggested that the median lethal and high lethal concentrations of triflumezopyrim inhibited the reproduction and reduced the expression of NlVg and JH III in N. lugens. This study provided a reference for the efficient and scientific utili zation of triflumezopyrim in the field.

A study of predicting irradiation-induced transition temperature shift for RPV steels with XGBoost modeling

Xu, Chaoliang,Liu, Xiangbing,Wang, Hongke,Li, Yuanfei,Jia, Wenqing,Qian, Wangjie,Quan, Qiwei,Zhang, Huajian,Xue, Fei Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.8

The prediction of irradiation-induced transition temperature shift for RPV steels is an important method for long term operation of nuclear power plant. Based on the irradiation embrittlement data, an irradiation-induced transition temperature shift prediction model is developed with machine learning method XGBoost. Then the residual, standard deviation and predicted value vs. measured value analysis are conducted to analyze the accuracy of this model. At last, Cu content threshold and saturation values analysis, temperature dependence, Ni/Cu dependence and flux effect are given to verify the reliability. Those results show that the prediction model developed with XGBoost has high accuracy for predicting the irradiation embrittlement trend of RPV steel. The prediction results are consistent with the current understanding of RPV embrittlement mechanism.

Irradiation-induced BCC-phase formation and magnetism in a 316 austenitic stainless steel

Xu, Chaoliang,Liu, Xiangbing,Xue, Fei,Li, Yuanfei,Qian, Wangjie,Jia, Wenqing Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.3

Specimens of austenitic stainless steel were irradiated with 6 MeV Xe ions to two doses of 7 and 15 dpa at room temperature and 300 ℃ respectively. Then partial irradiated specimens were subsequently thermally annealed at 550 ℃. Irradiation-induced BCC-phase formation and magnetism were analyzed by grazing incidence X-ray diffraction (GIXRD) and vibrating sample magnetometer (VSM). It has been shown that irradiation damage level, irradiation temperature and annealing temperature have significant effect on BCC-phase formation. This BCC-phase changes the magnetic behavior of austenitic stainless steel. The stress relief and compositional changes in matrix are the driving forces for BCC-phase formation in austenitic stainless steel during ion irradiation.

SOX12 Promotes Thyroid Cancer Cell Proliferation and Invasion by Regulating the Expression of POU2F1 and POU3F1

Zhenxi Su,Wenqing Bao,Guanghua Yang,Jianping Liu,Bin Zhao 연세대학교의과대학 2022 Yonsei medical journal Vol.63 No.6

Purpose: SOX12 is overexpressed in many cancers, and we aimed to explore the biological function and mechanism of SOX12 inthyroid cancer. Materials and Methods: We first analyzed the expression of SOX12 in thyroid cancer using data in The Cancer Genome Atlas. Immunohistochemistryand qRT-PCR were performed to identify SOX12 expression in thyroid cancer tissue and cells. Thyroid cancercells were transfected with small interfering RNA targeting SOX12, and cellular functional experiments, including CCK8, woundhealing, and Transwell assays, were performed. Protein expression was examined by Western blot analysis. A xenograft model wasdeveloped to evaluate the effect of SOX12 on tumor growth in vivo. Results: SOX12 expression was increased in thyroid cancer tissue and cells. SOX12 promoted cell proliferation, migration, and invasionand accelerated tumor growth in vivo. The expression of PCNA, Cyclin D1, E-cadherin, Snail, MMP-2, and MMP-9 was affectedby SOX12 knockdown. Bioinformatic analysis showed that SOX12 could interact with the POU family. SOX12 knockdown inhibitedthe expression of POU2F1, POU2F2, POU3F1 and POU3F2, and SOX12 expression showed a positive correlation with POU2F1,POU3F1, and POU3F2 expression in clinical data. POU2F1 and POU3F1 were able to reverse the effect of SOX12 knockdown onthyroid cancer cells. Conclusion: SOX12 affects the progression of thyroid cancer by regulating epithelial-mesenchymal transition and interacting withPOU2F1 and POU3F1, which may be novel targets for thyroid cancer molecular therapy.

Optimization method of vehicle handling stability based on response surface model with D-optimal test design

Bo Li,Wenqing Ge,Dechuan Liu,Cao Tan,Binbin Sun 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.6

In order to improve the handling stability of a vehicle, an optimization method of vehicle handling stability based on D-optimal test design was proposed in this paper. The multibody dynamic model was established and verified by experiments. On this basis, a response surface model was established based on D-optimal test design. An improved genetic-particle swarm algorithm was used to optimize the vehicle handling stability. The general evaluation score of vehicle handling stability was taken as the optimization objective. The vehicle structural parameters, tire and spring characteristics were regarded as design variables. The results showed that the multi-body dynamic model was accurate. After optimization, the general evaluation score of vehicle handling stability increased by 8.98 %; the score of the steering returnability and steady static circular test was increased by 20.43 % and 27.31 %, respectively. Then from the sensitivity of the optimization variables to the stability of the vehicle's handling, the rear wheel lateral stiffness has the greatest impact on it, with a sensitivity of 86.9 %; the wheelbase has the smallest impact on it, with a sensitivity of -3.39 %, which can be reduced in future optimization variable to improve design efficiency.

Effect of Scandium in Al–Sc and Al–Sc–Zr Alloys Under Precipitation Strengthening Mechanism at 350 °C Aging

Xue Na,Liu Wenqing,Zhu Liu,T. Muthuramalingam 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12

In the present scenario, it is much needed of developing Al–Sc and Al–Sc–Zr alloys owing to its higher strength to weightfor aerospace applications. The present study is dealt about effect of Scandium on micro hardness and micro structure ofsynthesized Al–Sc alloy and Al–Sc–Zr alloy under different weight percentage. The precipitation strengthening of as-caststructure, Al–Sc alloy and Al–Sc–Zr alloy have been analyzed during aging at 350 °C. From the experimental investigation,only little difference in micro hardness of developed alloys was observed during initial stage of aging. The peak hardnessof Al–Sc–Zr alloy was maintained for a relatively longer period for producing better thermal stability. Three-dimensionalatomic probing indicated that the precipitation of Zr in the precipitate at the initial stage of aging which can inhibit thegrowth of the precipitate’s size. TEM results showed that the Al–Sc–Zr alloy formed two precipitates of different sizes atthe over-aging phase after the peak state of hardness.

Recovery of zinc and iron from high iron-bearing zinc calcine by selective reduction roasting

Junwei Han,Wei Liu,Wenqing Qin,Bing Peng,Kang Yang,Yongxing Zheng 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.22 No.-

A novel method to recover zinc and iron from high iron-bearing zinc calcine by reduction roasting-lowacid leaching-magnetic separation was proposed. The effects of roasting and leaching conditions wereinvestigated and their optimum conditions were established. Meanwhile, the mineralogical changesduring the processes were characterized by XRD, VSM, SEM and LPSA. The results show the ZnFe2O4 inthe calcine was decomposed into ZnO and Fe3O4, and about 92.6% Zn was extracted from the roastedcalcine with reporting most of iron as magnetite into the residue. Finally, more than 90% Fe wasrecovered from the residue by magnetic separation method.

RGB tricolor produced by white-based top-emitting organic light-emitting diodes with microcavity structure

Jin Cao,Xiang Liu,M.A. Khan,WenQing Zhu,XueYin Jiang,ZhiLin Zhang,ShaoHong Xu 한국물리학회 2007 Current Applied Physics Vol.7 No.3

RGB pixels by microcavity top-emitting organic light-emitting diode (TOLED) is benecial to both minimizing the loss of light andimproving the color purity and the eciency. Based on the multi-emitting layers, white organic light-emitting diodes (OLEDs) andmicrocavity TOLEDs were prepared. TOLEDs were fabricated using Ag/ITO as the reector and adjusting layer, Al/Ag as semi-trans-cavity and the color of the device have been changed. So we get RGB tricolor devices. The peak wavelengths are 476 nm, 539 nm,601 nm, Commission Internationale d’Eclairage (CIE) coordinates are (0.133,0.201), (0.335,0.567), (0.513,0.360), FWHM are 32 nm,50 nm, 73 nm for blue, green and red, respectively.

Resonance-based sparse improved fast independent component analysis and its application to the feature extraction of planetary gearboxes

Jing Zhu,Aidong Deng,Jing Li,Minqiang Deng,Wenqing Sun,Qiang Cheng,Yang Liu 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.11

A resonance-based sparse improved fast independent component analysis (ICA) (RSIFICA) is proposed to extract the fault characteristics of planetary gearbox. First, the signal is decomposed using resonance sparse signal decomposition (RSSD). Second, highresonance components were retained while others were eliminated. Finally, the signal after dimension reduction was analyzed using ICA, and the fault characteristic frequency was extracted through envelope spectrum analysis. In this process, the preset Q parameter of RSSD is optimized on the basis of fuzzy entropy and ant-lion optimization algorithm. The accuracy of RSSD was improved by performing time-frequency entropy component selection. FastICA was improved, and the slow convergence problem of ICA was solved. Results showed that RSIFICA could extract the fault characteristic frequency accurately, and the calculation efficiency of FastICA increased by 21.49 %. In terms of extracting the fault features, its performance could be better than EMD-FastICA.