A Population-based Case-control Study on Risk Factors for Gastric Cardia Cancer in Rural Areas of Linzhou

Sun, Chang-Qing,Chang, Yu-Bo,Cui, Ling-Ling,Chen, Jia-Jun,Sun, Nan,Zhang, Wei-Jie,Jia, Xiao-Can,Tian, Yuan,Dai, Li-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Gastric cancer is the second most common cause of cancer-related deaths in the world. Although certain dietary factors and lifestyles have been suggested to be associated with gastric carcinogenesis, there have been few investigations focusing on rural areas. A case-control study was therefore carried out to investigate the risk factors of gastric cardia cancer (GCC) in rural areas of Linzhou. A total of 470 newly diagnosed cases of GCC and 470 healthy controls were included. Face-to-face interviews were conducted, using a uniform questionnaire containing questions on demographics, per capita income, living habits, dietary habits and family history of tumors. The relationship between putative risk factors and GCC was assessed by odds ratios (OR) and their 95% confidence intervals (95%CI) derived from conditional logistic regression model by the COXREG command using SPSS 12.00. Multivariate logistic regression analysis was used to evaluate simultaneously the effects of multiple factors and other potential confounding factors. Multivariate logistic analysis showed that smoking (OR=1.939, 95%CI:1.097-3.426), alcohol drinking (OR=2.360, 95%CI: 1.292-4.311), hot food consumption (OR=2.034, 95%CI: 1.507-2.745), fast eating (OR=1.616, 95%CI: 1.171-2.230), mouldy food (OR=4.564, 95%CI: 2.682-7.767), leftover food (OR=1.881. 95%CI: 1.324-2.671), and family history of tumor (OR=2.831, 95%CI: 1.588-5.050) were risk factors for GCC. High per capita income (OR=0.709, 95%CI: 0.533-0.942), high education level (OR=0.354, 95%CI: 0.163-0.765), consumption of fresh fruits (OR=0.186, 95%CI: 0.111-0.311) and vegetables (OR=0.243, 95%CI: 0.142-0.415), and high BMI (OR=0.367, 95%CI: 0.242-0.557) were protective factors for GCC. Our data indicate that unhealthy lifestyle and dietary habits might be important contributors to GCC in this population.

Molecular cloning of the duck MEF2C gene cDNA coding domain sequence and its expression during fetal muscle tissue development

Ling-Li Sun,He-he Liu,Hao-han Wang,Jian-Ming Si,Hai-bo Jin,Xin-xin Li,Chao Yang,Liang Li,Jiwen Wang 한국유전학회 2013 Genes & Genomics Vol.35 No.3

Myogenic enhancer transcription factor 2c (MEF2c), one of the members of the MEF2 family of transcription factors, plays an important role in mammalian muscle development. However, the role of MEF2c in avian muscle development still remains unclear. To understand the function of MEF2c in avian muscle development, we first cloned the duck MEF2c coding domain sequence (CDS) and analyzed MEF2c expression in duck muscle tissues of embryos from 10 days of incubation to 1 week after birth using real-time PCR technology. The results showed that the duck MEF2c CDS consists of 1,398nucleotides that encode 465 amino acids. The MEF2c duck protein contains a MADS domain, a MEF2 domain and a HJURP_C domain with high homology to related proteins in other organisms. Different expression levels of MEF2c were found in skeletal, smooth and cardiac muscle. Therefore, these results indicated that duck MEF2c has two conserved domains (a MADS and a MEF2 domain), is an indispensable regulator of muscle development, and plays an important role in the development of duck muscle.

Contact modeling and stiffness of a rough surface under mixed lubrication condition

Ling Li,Litai Sun,Jingjing Wang,Benshuai He,Chongqing Fan,Lixia Li,Miaoxia Xie 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.2

A method of using a quadratic function of rotary asperities to be equivalent to asperity is established based on a measurement experiment on rough surface topography. A micro-contact model of a rough surface that includes elastic, elastic-plastic, and pure plastic deformation states is re-derived using the theory of contact mechanics and the method of probability statistics. A rough surface contact stiffness model under mixed lubrication is proposed in accordance with the rough surface contact stiffness, which consists of solid surface and lubrication medium contact stiffness. The influence of the lubrication medium, solid material, and asperity size parameters on contact stiffness is analyzed. Results show that elastic modulus, acoustic impedance, and the size parameters of the asperities of the solid part are the main factors that affect the contact stiffness of the solid part. The acoustic impedance of the lubricating medium mainly affects the contact stiffness of the lubrication part. The contact stiffness of the rough surface under mixed lubrication is a superposition of the solid and lubrication parts, and it is greater than that without the lubrication medium. The model established in this study fully considers the deformation state in practical engineering, which includes asperities and the lubrication state of rough surfaces. It provides a reference for the performance prediction of mechanical surfaces.

Toward better translation of clinical research evidence into rapid recommendations for traditional Chinese medicine interventions: A methodological framework

Sun Xin,Luo Xiaochao,Li Ling,Ma Bin,Yao Minghong,Liu Jiali,Ge Long,Chen Xiaofan,Wu Xi,Deng Hongyong,Zhou Xu,Wen Zehuai,Li Guowei,Li Qianrui 한국한의학연구원 2022 Integrative Medicine Research Vol.11 No.3

Rapid recommendation is a novel methodological framework for developing clinical practice guidelines and this framework shares the basic features of classical guidelines but differs from classical clinical practice guidelines in its ‘rapid’ development process (typically within 90 days) with an aim of translat-ing practice-changing studies to recommendations. A recent global innovation of guideline development methodology is the proposal of a rapid recommendation framework for Traditional Chinese Medicine (TCM), which has the potential to add value to the translation of evidence to practice for TCM inter- ventions. Up to now, more than 180 rapid recommendations have been published, but none of them is pertaining to TCM interventions. Due to the nature of multi-dimensional evidence sources for TCM inter- ventions, including classical randomized controlled trials and real world evidence, a more sophisticated methodological approach to synthesize and evaluate the totality of evidence about effects of TCM in- terventions is required. Therefore, appropriate modification to the rapid recommendation framework is necessary. In the efforts to respond to these needs, we have proposed a specific approach to developing rapid recommendations for TCM interventions the Multi-dimensional Evidence Synthesis, Evaluation and Recommendations for TCM interventions (MESERT)

Growth of Ni/Co-Catalyzed Crystalline CNx Thin Films by Nitrogen-Plasma-Assisted Pulsed Laser Deposition

Li Li,Ning Xu,Aimin Wu,Hao Ling,Jian Sun,Xiaokang Shen 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.46 No.1

Nanocrystalline carbon nitride (CNx) thin ¯lms have been synthesized by nitrogen-plasma-assisted pulsed-laser deposition. Before depositing carbon nitride ¯lm, Ni/Co was deposited on the silicon (100) substrate acting as catalyst. The properties of the deposited ¯lms were studied by several diagnostic techniques. X-ray photoelectron spectroscopy was used for detecting the compo-sition ratio N/C in the ¯lm. From studying Raman spectra under dierent conditions we can ¯nd how glow discharge pressure aects the carbon nitride ¯lm properties. Two stages in this process and nanocrystallites of tens of nanometers embedded in the as-synthesized thin ¯lm can be seen by transmission-electron microscopy. The FTIR spectrum suggests an abundance of C-N bonds and paucity of C´N bonds, which is a favorable chance for the formation of carbon nitride crystallites. The crystalline nature of the ¯lm is detected by X-ray diraction.

Sevoflurane induces neuronal apoptosis via enhancing DNMT3L expression and promoting methylation of PSD95 promoter in postoperative cognitive dysfunction

Ling Yunzhi,Li Xiaohong,Yu Li,Sun Yiyun,Yang Dongdong,Li Zhiyi 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.3

Background Postoperative cognitive dysfunction (POCD) is a central nervous system (CNS) complication and occurs quite frequently after anesthesia, especially in elderly patients. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is an enzymatically inactive regulatory factor that can modulate gene expression via methylation. Few studies specifically focused on the role of DNMT3L in sevoflurane-induced POCD. Objective The cognitive dysfunction was determined by Morris water maze assay. Hippocampal neuron apoptosis was measured by TdT-mediated dUTP nick-end labeling (TUNEL) assay. Neuron proliferation and apoptosis were measured by cell counting kit-8 (CCK-8) and flow cytometry assay. The expression levels of DNMT3L and postsynaptic density protein-95 (PSD95) were measured by qRT-PCR and western blot. Results The sevoflurane-induced POCD aging rat model was established for in vivo study. Suppression of DNA methylation by 5-Aza-2′-deoxycytidine (5-AZA; a widely used methylation inhibitor) reversed sevoflurane-induced cognitive dysfunction and hippocampal neuron apoptosis in rats. Moreover, sevoflurane enhanced DNMT3L expression and induced methylation of PSD95 promoter in vivo. In vitro, sevoflurane induced neuronal apoptosis and DNMT3L expression to promote PSD95 methylation. Finally, rescue experiments indicated that sevoflurane induced neuron apoptosis by increasing DNMT3L expression and promoting methylation of PDS95 in POCD. Conclusion Sevoflurane induced neuronal apoptosis by increasing DNMT3L expression and promoting methylation of PDS95 in vivo and in vitro, providing a novel therapeutic strategy for POCD.

Electrochemical Polymerization of Methoxy Polyethylene Glycol Acrylate on Carbon Fiber for Improved Interfacial Properties

Qiu Li,Ai-ling Zhang,Meng Zhang,Fang-fang Li,Song Wang,San-xi Li,Yong-jiang Li,Ze-fei Sun 한국섬유공학회 2019 Fibers and polymers Vol.20 No.12

Methoxy polyethylene glycol acrylate (MPGA) was electrochemical polymerized on carbon fiber (CF) by usingcyclic voltammetry (CV) to enhance the surface properties of CF. Fourier transform infrared spectroscopy (FTIR), scanningelectron microscopy (SEM) and thermogravimetry analysis (TGA) investigations confirmed that MPGA electropolymerizedon CF (MPGA-CF) was obtained. The low frequency capacitance of MPGA-CF (CLF=5.85 mF·cm-2) was higher thanCF(CLF=4.75 mF·cm-2) based on the result of electrochemical impedance spectroscopy(EIS) and the data obtained fitted withan R(Q(R(Q(R(CR))))) equivalent circuit model. Contact angle experiment showed that the contact angle between water andMPGA-CF is significantly lower than that between water and CF. Mechanical experimental results indicated that theinterlaminar shear strength (ILSS) of MPGA-CF/epoxy resin composites (32.24 MPa) has improved by 134.81 % comparewith the CF/epoxy resin composites (13.73 MPa), which significantly enhanced interfacial properties of epoxy resincomposites.

원저 : 한국인 턱뼈관의 국소해부

정려령 ( Li Ling Zheng ) , 유선경 ( Sun Kyoung Yu ) , 이명화 ( Myoung Hwa Lee ) , 이백희 ( Baek Hee Lee ) , 김흥중 ( Heung Joong Kim ) 조선대학교 구강생물학연구소 2009 Oral Biology Research (Oral Biol Res) Vol.33 No.3

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Mixtures of Tea and Citrus maxima (pomelo) Alleviate Lipid Deposition in HepG2 Cells Through the AMPK/ACC Signaling Pathway

Ran An,Shuai Wen,Dong-Li Li,Qiu-Hua Li,Xing-Fei Lai,Wen-Ji Zhang,Ruo-Hong Chen,Jun-Xi Cao,Zhi-Gang Li,Qiu-Sheng Huang,Ling-Li Sun,Shi-Li Sun 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.9

Tea and citrus maxima are natural, medicinal homologous plants, typically used for making beverages, which have anticancer, antiobesity, and antioxidation properties. Green tea, yellow tea, and black tea were combined with citrus maxima to obtain green tea and Citrus maxima (GTCM), yellow tea and Citrus maxima (YTCM), and black tea and Citrus maxima (BTCM). The biochemical components of these mixtures were analyzed, and their possible effects and mechanisms on relieving liver lipid deposition were explored. The tea polyphenols, free amino acids, phenolamine ratio, and caffeine were comparable in YTCM and GTCM, being significantly higher than those in BTCM. In addition, the content of esterified catechins, nonesterified catechins, and total catechins in YTCM was significantly higher than those in GTCM and BTCM. All three mixtures of Citrus maxima tea significantly reduced lipid deposition in HepG2 cells, with GTCM and YTCM being slightly more effective than BTCM. Regarding the possible mechanism, Western blot analysis revealed that the three Citrus maxima tea mixtures could activate the AMPK/ACC signaling pathway, upregulate the expression of p-AMPK, p-ACC, and CPT-1 proteins, and downregulate the expression of SREBP1c and fatty acid synthase proteins to inhibit fat synthesis, thereby relieving lipid deposition in liver cells. In conclusion, as a novel and healthy beverage, Citrus maxima tea has the potential to alleviate liver lipid deposition, and further could be responsible for obesity treatment.

Pretreatment Serum Amyloid A and C-reactive Protein Comparing with Epstein-Barr Virus DNA as Prognostic Indicators in Patients with Nasopharyngeal Carcinoma: A Prospective Study

Qiu-Yan Chen,Qing-Nan Tang,Lin-Quan Tang,Wen-Hui Chen,Shan-Shan Guo,Li-Ting Liu,Chao-Feng Li,Yang Li,Yu-Jing Liang,Xue-Song Sun,Ling Guo,Hao-Yuan Mo,Rui Sun,Dong-Hua Luo,Yu-Ying Fan,Yan He,Ming-Yuan C 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3

Purpose The measuring Epstein-Barr virus (EBV) DNA is an important predictor of nasopharyngeal carcinoma (NPC). This study evaluated the predictive value of pretreatment serum amyloid A (SAA) and C-reactive protein (CRP) comparing with EBV DNA in patients with NPC. Materials and Methods In an observational study of 419 non-metastatic NPC patients, we prospectively evaluated the prognostic effects of pretreatment SAA, CRP, and EBV DNA on survival. The primary endpoint was progress-free survival (PFS). Results The median level of SAA and CRP was 4.28 mg/L and 1.88 mg/L, respectively. For the high- SAA group (> 4.28 mg/L) versus the low-SAA ( 4.28 mg/L) group and the high-CRP group (> 1.88 mg/L) versus the low-CRP ( 1.88 mg/L) group, the 5-year PFS was 64.5% versus 73.1% (p=0.013) and 65.2% versus 73.3% (p=0.064), respectively. EBV DNA detection showed a superior predictive result, the 5-year PFS in the EBV DNA  1,500 copies/mL group was obviously different than the EBV DNA < 1,500 copies/mL group (62.2% versus 77.8%, p < 0.001). Multifactorial Cox regression analysis confirmed that in the PFS, the independent prognostic factors were including EBV DNA (hazard ratio [HR], 1.788; p=0.009), tumour stage (HR, 1.903; p=0.021), and node stage (HR, 1.498; p=0.049), but the SAA and CRP were not included in the independent prognostic factors. Conclusion The results of SAA and CRP had a certain relationship with the prognosis of NPC, and the prognosis of patients with high level of SAA and CRP were poor. However, the predictive ability of SAA and CRP was lower than that of EBV DNA.