Electrochemical preparation of Co₃Pt nanowires

Min, Ji Hyun,Wu, Jun Hua,Cho, Ji Ung,Lee, Ju Hun,Ko, Young-Dong,Liu, Hong-Ling,Chung, Jin-Seok,Kim, Young Keun WILEY-VCH Verlag 2007 Physica status solidi. PSS. A, Applications and ma Vol.204 No.12

<P>Fabrication of Co-Pt nanowire arrays with fcc-Co<SUB>3</SUB>Pt phase was accomplished by DC electrodeposition in anodic aluminum oxide (AAO) nanotemplates. The microstructural and magnetic properties of the nanowires were investigated as a function of the deposition current density (Co concentration). The nanowires possess fcc-Co<SUB>3</SUB>Pt phases and are ferromagnetic. Their crystallinity and magnetic performance was enhanced after annealing. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)</P>

Electrochemical Preparation of Co₃Pt Nanowires

Ji Hyun Min,Jun-Hua Wu,Ji Ung Cho,Ju Hun Lee,Young-Dong Ko,Seung Pil Ko,Hong-Ling Liu,Jin-Seok Chung,Young Keun Kim 한국자기학회 2007 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

B3GNT3 acts as a carcinogenic factor in endometrial cancer via facilitating cell growth, invasion and migration through regulating RhoA/RAC1 pathway‑associated markers

Ji-Shui Wang,Fang Ruan,Li-Zhu Guo,Feng-Ge Wang,Fu-Ling Wang,Hong-Min An 한국유전학회 2021 Genes & Genomics Vol.43 No.5

Background Aberrant expression of beta-1,3-N-acetylglucosaminyltransferase-3 (B3GNT3) has been frequently clarifed in various cancers, however, its role in endometrial cancer (EC) has not been assessed in detail. Purpose This study aimed to investigate the biological role of B3GNT3 in EC and simply explored the detailed mechanism. Methods The EC RNA-Seq dataset from TCGA database was applied to evaluate the expression of B3GNT3 and assess its role on prognostic value. HEC-1-A and KLE cell lines of EC were used to perform loss- and gain-of-function B3GNT3 assays respectively. Quantitative real-time PCR (qRT-PCR) and western blot were used to measure the mRNA and protein levels of indicated molecules respectively. Cell counting kit-8, clone formation tests, and Transwell assay served to determine the changes of proliferative, invasive and migratory abilities of EC cells after altering the expression of B3GNT3. Results B3GNT3 was found to be highly expressed in EC tissues compared to normal tissues according to the online public databases, which confrmed by the following qRT-PCR in 3 EC cell lines. Besides, high B3GNT3 expression presented a worse overall survival in EC patients as compared with low B3GNT3 expression group. Furthermore, functional experiments in vitro indicated that B3GNT3 could facilitate the cell growth, invasion and migration. Moreover, we found that downregulation of B3GNT3 signifcantly reduced the expression level of GTP-RhoA and GTP-RAC1, whereas upregulation of B3GNT3 presented the opposite results. Conclusion The results of current study demonstrate that B3GNT3 acts as an oncogene that promotes EC cells growth, invasion and migration possibly through regulating the RhoA/RAC1 signaling pathway-related markers, suggesting that B3GNT3 may be a candidate biomarker for EC therapeutic intervention.

Fabrication of Multifunctional Au Doped CoPt Nanowires

Ji Hyun Min,Hong-Ling Liu,Ju Hun Lee,Jun-Hua Wu,Jae-Seon Ju,Young Keun Kim IEEE 2009 IEEE transactions on magnetics Vol.45 No.6

<P>Au-doped CoPt nanowires were prepared in anodized aluminum oxide (AAO) nanotemplates by electrodeposition and the doping effects on their microstructure and magnetic properties were investigated. Dependent on the deposition conditions, the CoPtAu nanowires can have the cubic or tetragonal phase. The magnetic measurements indicate that the nanowires demonstrate ferromagnetic behavior, showing the easy axis along the direction of the nanowires and strong enhancement in coercivity and squareness prior to annealing, whereas the optical measurements reveal plasmon resonances characteristic of nanostructured Au.</P>

Magnetic-Optical AuFe Alloy Nanoparticles

Hong Ling Liu,Jun Hua Wu,Ji Hyun Min,Young Keun Kim 한국자기학회 2007 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Smart nanoparticles (NPs) of multiple components offer exciting opportunities in fundamental studies and highly multidisciplinary nanotechnology that has rapidly grown with tremendous applications in many areas including medicine, life science, materials science, environment, electronics, and energy. Metallic alloy NPs have been extensively investigated for various purposes such as magnetic recording, drug delivery, bioseparation, medical diagnosis, and catalysis, particularly magnetic and optical properties of materials are widely pursued. Since fusion of the magnetic and optical elements in one single entity promises multifunctionality and potential applications, so a great deal of effort is instilled to prepare such nanoparticles containing Au and Fe. We have investigated a variety of functional nanoparticles via an efficient, scalable and non-toxic synthesis approach [1]. In this work, we report the preparation and characterization of multifunctional magnetic-optical AuFe alloy nanoparticles, integrating the optical functionality of Au composition and the magnetic properties of iron. The nanoparticles were synthesized by a modified polyol process using iron acetylacetonate (Fe(acac)3) and gold acetate (Au(ac)3) in the presence of surfactants at high temperature[1].Three compositions of AuFe nanoparticles, Au0.25Fe0.75, Au0.5Fe0.5, and Au0.75Fe0.25, were formed and investigated on the structure and properties as a function of composition by TEM/HRTEM, XRD, UV-vis, XPS and VSM/SQUID. It is found that the fusion of the two materials into one nanostructure entity retains the optical and magnetic properties of the individual components (Figs.1,2). The XRD and TEM analysis confirms the formation of the alloy nanostructure and provides the detailed structural arrangements, with a narrow distribution of particle sizes in the range of 5~10 ㎚ (Figs.34). X-ray photoelectron spectroscopy reveals the binding energies of Au and Fe in the state of the AuFe alloy nanoparticles. The magnetic characterization shows the superparamagnetic or soft ferromagnetic behavior (Fig.1) of the nanoparticles at room temperature, while the thermal response to different external fields was obtained from the M~T measurements in the FC and ZFC modes, respectively. The UV-vis spectra display the variation of the absorption bands at ~560㎚ with composition (Fig.2), which is characteristic of nanostructured gold. These AuFe nanoparticles are water soluble after thiolation.

Research Articles : Genetic analysis of the related traits of flowering and silk for hybrid seed production in maize

( Hui Ling Xie ),( Dong Ding ),( ZI Tian Cui ),( Xin Wu ),( Yan Min Hu ),( Zong Hua Liu ),( Yu Ling Li ),( Ji Hua Tang ) 한국유전학회 2010 Genes & Genomics Vol.32 No.1

Multifunctional Magnetic-luminescent CoPt-CdSe Core-shell Nano-particles

Hong Ling Liu,Jun Hua Wu,Ju Hun Lee,Boo Hyun An,Ji Hyun Min,Do Kyun Kim,Young Keun Kim 한국자기학회 2007 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

톳 유래 저분자 푸코이단의 면역활성 증진

하지혜(Ji Hye Ha),권민철(Min Chul Kwon),한재건(Jae Gun Han),김령(Ling Jin),정향숙(Hyang Suk Jeong),최근표(Geun Pyo Choi),박욱연(Uk Yeon Park),유상권(Sang Guan You),이현용(Hyeon Yong Lee) 한국식품과학회 2008 한국식품과학회지 Vol.40 No.5

본 연구는 톳에서 푸코이단의 추출시 초음파 공정을 이용하여 처리함으로써 유래 푸코이단의 저분자화를 통한 면역활성 및 항암활성의 증진 효과를 알아보고자 톳 유래 푸코이단 및 저분자 푸코이단(분자량 14,000 이하)을 이용하여 수행하고, 푸코이단 표준물질과 비교하였다. 시료 첨가를 통한 면역 B세포와 T세포의 생육도에서 톳 유래 저분자 푸코이단이 1.0 ㎎/mL의 농도에서 무첨가 대조구와 비교해 각각 367%와 387%의 생육도를 나타내며 가장 높은 활성을 나타내었다. 면역세포의 cytokine 분비량 측정에서도 생육도 측정에서와 유사한 결과를 나타내며 저분자 푸코이단이 가장 많은 cytokine 분비량을 나타내었으며, 이는 NK 세포의 생육에도 영향을 끼치는 것으로 사료되는 바, NK 세포 생육 증진에서도 가장 높은 수치를 나타냈다. 인간 정상세포를 이용한 세포독성 측정에서는 시료 간 큰 차이를 보이지는 않았으나 1.0 ㎎/mL에서 푸코이단 표준물질이 21.45%로 가장 높은 수치를 나타내었고, 저분자된 톳 유래 푸코이단이 19%로 가장 낮은 수치를 나타내었다. 인간폐암세포인 A549와 유방암세포인 MCF-7에 대한 생육억제 활성 측정에서도 저분자 푸코이단이 1.0 ㎎/mL의 농도에서 각각 69.8%와 83.3%를 나타내었으며, 푸코이단 표준물질도 55% 이상의 활성을 나타내었다. 이상의 결과를 통해 푸코이단 시료는 저분자화를 통해 면역 및 항암활성의 증진이 가능함을 확인하였다. The anticancer and immunomodulatory activities of low molecular weight (Mw 11 kDa) fucoidan isolated from Hizikia fusiforme (H. fusiforme) via the ultrasonification extraction process were assessed in this study. Low molecular weight fucoidan improved the growth of human B and T cells, up to approximately 40% as compared to the controls (untreated) and 30% for commercially available fucoidan (Mw 150 kDa). IL-6 and TNF-α were secreted from human B cells at levels of 7.8×10?⁴ pg/mL and 7.2×10?⁴ pg/mL, respectively, and these levels were higher than the levels measured in the controls and with other high molecular weight fucoidan. It was also determined that the cytokine from human B and T cells cultivated with added fucoidan enhanced the growth of human NK cells. The fucoidan isolated from H. fusiforme showed low cytotoxicity, approximately 19%, after the addition of 1.0 ㎎/mL, the highest tested concentration. The growth of human lung cancer cells (A549) and human breast cancer cells (MCF-7) were inhibited by 69.8% and 83.3%, respectively. These results demonstrated that the low molecular weight fucoidan isolated from H. fusiforme has potential as a new functional food component that evidences immunomodulatory activities and anticancer activity. One of the primary positive features of this fucoidan is that low molecular weight polysaccharides can be readily handled during processing.

Differential Impacts on Bacterial Composition and Abundance in Rhizosphere Compartments between Al-Tolerant and Al-Sensitive Soybean Genotypes in Acidic Soil

Wen Zhong-Ling,Yang Min-Kai,Fazal Aliya,Liao Yong-Hui,Cheng Lin-Run,Hua Xiao-Mei,Hu Dong-Qing,Shi Ji-Sen,Yang Rong-Wu,Lu Gui-Hua,Qi Jin-Liang,Zhi Hong,Qian Qiu-Ping,Yang Yong-Hua 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.8

In this study, two soybean genotypes, i.e., aluminum-tolerant Baxi 10 (BX10) and aluminumsensitive Bendi 2 (BD2), were used as plant materials and acidic red soil was used as growth medium. The soil layers from the inside to the outside of the root are: rhizospheric soil after washing (WRH), rhizospheric soil after brushing (BRH) and rhizospheric soil at two sides (SRH), respectively. The rhizosphere bacterial communities were analyzed by high-throughput sequencing of V4 hypervariable regions of 16S rRNA gene amplicons via Illumina MiSeq. The results of alpha diversity analysis showed that the BRH and SRH of BX10 were significantly lower in community richness than that of BD2, while the WRH exhibited no significant difference between BX10 and BD2. Among the three sampling compartments of the same soybean genotype, WRH had the lowest community richness and diversity while showing the highest coverage. Beta diversity analysis results displayed no significant difference for any compartment between the two genotypes, or among the three different sampling compartments for any same soybean genotype. However, the relative abundance of major bacterial taxa, specifically nitrogen-fixing and/or aluminum-tolerant bacteria, was significantly different in the compartments of the BRH and/or SRH at phylum and genus levels, indicating genotype-dependent variations in rhizosphere bacterial communities. Strikingly, as compared with BRH and SRH, the WRH within the same genotype (BX10 or BD2) always had an enrichment effect on rhizosphere bacteria associated with nitrogen fixation

Monosized core-shell Fe3O4(Fe)/Au multifunctional nanocrystals.

Liu, Hong-Ling,Wu, Jun-Hua,Min, Ji Hyun,Lee, Ju Hun,Kim, Young Keun American Scientific Publishers 2009 Journal of nanoscience and nanotechnology Vol.9 No.2

<P>We have synthesized highly crystalline, monosized Fe3O4(Fe)/Au magnetic-optic multifunctional nanocrystals using a modified polyol process. The formation of the core-shell structure was achieved in two consecutive steps, seeding the Fe3O4(Fe) core followed by coating the gold shell. The characterization demonstrates the core-shell nanostructure and provides particle size and distribution of the nanocrystals. The spectroscopic analysis gives three Fe oxidation states (Fe0, Fe+2 and Fe+3) in the core and the surface plasmon characteristic of the Au nanoshell, while the magnetic measurements show excellent susceptibility of the nanocrystals.</P>