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Ding Sheng-Long,Zhang Tai-Wei,Zhang Qi-Chen,Ding Wang,Li Ze-Fang,Han Guan-Jie,Bai Jin-Song,Li Xi-Lei,Dong Jian,Wang Hui-Ren,Jiang Li-Bo 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
Night shift workers with disordered rhythmic mechanical loading are more prone to intervertebral disc degeneration (IDD). Our results showed that circadian rhythm (CR) was dampened in degenerated and aged NP cells. Long-term environmental CR disruption promoted IDD in rats. Excessive mechanical strain disrupted the CR and inhibited the expression of core clock proteins. The inhibitory effect of mechanical loading on the expression of extracellular matrix genes could be reversed by BMAL1 overexpression in NP cells. The Rho/ROCK pathway was demonstrated to mediate the effect of mechanical stimulation on CR. Prolonged mechanical loading for 12 months affected intrinsic CR genes and induced IDD in a model of upright posture in a normal environment. Unexpectedly, mechanical loading further accelerated the IDD in an Light-Dark (LD) cycle-disrupted environment. These results indicated that intrinsic CR disruption might be a mechanism involved in overloading-induced IDD and a potential drug target for night shift workers.
Revelation of the early responses of salt tolerance in maize via SSH libraries
Dong Ding,Zhixia Xiao,Hailin Xiao,Tao Xia,YongLian Zheng,Fazhan Qiu 한국유전학회 2012 Genes & Genomics Vol.34 No.3
Maize (Zea mays L.), one of the most important cereal crops in the world, is salt-sensitive. It is critical to study the genes associated with salt tolerance, the differential gene expression pattern between salt-tolerant and salt-sensitive lines, and the mechanism of salt tolerance in maize. Two maize inbred lines with different salt sensitivity, NC286 and Huangzao4, were used to reveal early responsive genes under high salinity condition. Suppression subtractive hybridization (SSH) assay was conducted to identify potential genes involved in the early stage of maize seedlings in responses to 200mM NaCl. A custom μparafloTM microfluidic array (LC science USA) containing Release version 10.1 plant miRNA probes (http://microrna. sanger.ac.uk/) was used to discover salt stress responsive miRNAs using the differences in miRNAs expression between these two maize lines. Cis-elemnet analysis was performed in the promoter regions of these two kinds of salt stress responsive transcriptions. The expression of a total of 141unique genes was significantly changed under 200mM NaCl salt shock within 24 hours. These genes were involved in a broad spectrum of biochemical, cellular, and physiological processes. They were assigned to 14 categories based on their biological functions. Some of them were previously reported to be associated with biotic and abiotic stresses, and several novel genes detected. A gene model was proposed which showed how these genes could sense and response to the high salinity environment. The promoter regions of these 141 salt responsive genes were analyzed, and salt responsive key cis-elements uncovered. The cis-elements usage bias of the salt responsive mRNAs and non-coding RNAs were of the same. The different expression profiles of the genes, especially the ones involved in salt signal cascades and phytohormones responding,could explain the different salt stress response in the two maize inbred lines. The similar cis-elements usage bias of two kinds of salt responsive transcriptions suggests they may be regulated by the same trans-factors.
Stability of caisson-type breakwater using coupled Fluid-Porous model
Ding, Dong,Ouahsine, Abdellatif,Huang, Zhaoyuan Techno-Press 2021 Coupled systems mechanics Vol.10 No.3
Breakwaters are used for the protection of harbors and beaches against wave action. This paper focuses on the analysis of the stability of the caisson-type breakwater under Flip-through wave impacts using a coupled Fluid-Porous model. The fluid hydrodynamic is described by the Volume-averaged Reynolds-Averaged Navier-Stokes (VARANS) equation with k-ε model. The flow in the porous medium and armour layer is simulated by the extended Forchheimerlaw. The developed model is used to estimate the influence of the thickness of armour layer and angle of wave return wall. Thus, a new relation of the overtopping discharge with the thickness of armour layer and angle of wave return wall is established, which can be used to design the structure of breakwater according to the limited value of overtopping wave discharge.
( Ding Hong Lei ),( Hao Zeng ),( Lin Ping Huang ),( Yan Dong Dong ),( Yi Jun Duan ),( Xu Hu Mao ),( Gang Guo ),( Quan Ming Zou ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.10
Most of the Helicobacter pylori strains containing the cag pathogenicity island (PAI) have been associated with more severe gastric disease in infected humans. The cag PAI is composed of 27 proteins, and some of the components are required for CagA translocation into host cells as well as induction of proinflammatory cytokines, such as interleukin-8 (IL-8); however, the exact function of most of the components remains unknown or poorly characterized. In this study, we demonstrated that CagT (HP0532), which is an essential structural component of the cag PAI apparatus, plays an important role in the translocation of CagA into host epithelial cells. In addition to being located on the bacterial surface, CagT is also partially localized in the inner membrane, where it acts as a chaperone-like protein and promotes CagA translocation. However, CagT secretion was not detected by immunoprecipitation analysis of cell culture supernatants. Meanwhile, CagT was related to the introduction of IL-8 of the host cell. These results suggest that CagT is expressed on both the inner and outer bacterial membranes, where it serves as a unique type IV secretion system component that is involved in CagA secretion and cag PAI apparatus assembly.
Yueyun Ding,Shujiao Zhu,Chaodong Wu,Li Qian,DengTao Li,Li Wang,Yuanlang Wang,Wei Zhang,Min Yang,Jian Ding,Xudong Wu,Xiao-Dong Zhang,Yafei Gao,Zongjun Yin 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.7
Objective: Mutations in low-density lipoprotein receptor (LDLR), which encodes a critical protein for cholesterol homeostasis and lipid metabolism in mammals, are involved in cardiometabolic diseases, such as familial hypercholesterolemia in pigs. Whereas microRNAs (miRNAs) can control LDLR regulation, their involvement in circulating cholesterol and lipid levels with respect to cardiometabolic diseases in pigs is unclear. We aimed to identify and analyze LDLR as a potential target gene of SSC-miR-20a. Methods: Bioinformatic analysis predicted that porcine LDLR is a target of SSC-miR-20a. Wild-type and mutant LDLR 3′-untranslated region (UTR) fragments were generated by polymerase chain reaction (PCR) and cloned into the pGL3-Control vector to construct pGL3 Control LDLR wild-3′-UTR and pGL3 Control LDLR mutant-3′-UTR recombinant plasmids, respectively. An miR-20a expression plasmid was constructed by inserting the porcine pre-miR-20a-coding sequence between the HindIII and BamHI sites in pMR-mCherry, and constructs were confirmed by sequencing. HEK293T cells were co-transfected with the miR-20a expression or pMR-mCherry control plasmids and constructs harboring the corresponding 3′-UTR, and relative luciferase activity was determined. The relative expression levels of miR-20a and LDLR mRNA and their correlation in terms of expression levels in porcine liver tissue were analyzed using reverse-transcription quantitative PCR. Results: Gel electrophoresis and sequencing showed that target gene fragments were successfully cloned, and the three recombinant vectors were successfully constructed. Compared to pMR-mCherry, the miR-20a expression vector significantly inhibited wild-type LDLR-3′-UTR-driven (p<0.01), but not mutant LDLR-3′-UTR-driven (p>0.05), luciferase reporter activity. Further, miR-20a and LDLR were expressed at relatively high levels in porcine liver tissues. Pearson correlation analysis revealed that porcine liver miR-20a and LDLR levels were significantly negatively correlated (r = –0.656, p<0.05). Conclusion: LDLR is a potential target of miR-20a, which might directly bind the LDLR 3′-UTR to post-transcriptionally inhibit expression. These results have implications in understanding the pathogenesis and progression of porcine cardiovascular diseases.
Data Filtering based Least Squares Algorithms for Multivariable CARAR-like Systems
Dong-Qing Wang,Feng Ding,Da-Qi Zhu 제어·로봇·시스템학회 2013 International Journal of Control, Automation, and Vol.11 No.4
This paper focuses on the identification problem of multivariable controlled autoregressive autoregressive (CARAR-like) systems. The corresponding identification model contains a parameter vector and a parameter matrix, and thus the conventional least squares methods cannot be applied to directly estimate the parameters of the systems. By using the hierarchical identification principle, this paper presents a hierarchical generalized least squares algorithm and a filtering based hierarchical least squares algorithm for the multivariable CARAR-like systems. The simulation results show that the two hierarchical least squares algorithms are effective.