Chemical Investigation of Volatiles Emitted from Flowers of Three Varieties of Damask Rose Cultivated in Beijing

Lina Yang,Jianwu Ren,Yan Wang 한국원예학회 2014 Horticulture, Environment, and Biotechnology Vol.55 No.6

Three varieties of Damask rose named ‘Four Seasons’, ‘Celsiana’ and ‘Rose de Rescht’, were studied inBeijing in dry and frozen climate. The volatiles emitted from flowers were collected by dynamic headspace collectionmethod and were detected and identified via thermal-desorption cold trap/gas chromatography/mass spectrometer technique(TCT-GC/MS). The results revealed that 63, 68 and 55 species of individual compounds were found from ‘FourSeasons’, ‘Celsiana’ and ‘Rose de Rescht’, respectively. The results demonstrated that ‘Celsiana’ possessed most diversevolatile components; the relative abundance of terpenes was as much as 49%, whereas it was 20 and 40% for ‘FourSeasons’ and ‘Rose de Rescht’, respectively. It meant that the rose oil from flowers of ‘Celsiana’ might be of highquality. Therefore, industrial plantation of ‘Celsiana’ is probably reasonable in Beijing area.

Comparison of Faecal Microbial Community of Lantang, Bama, Erhualian, Meishan, Xiaomeishan, Duroc, Landrace, and Yorkshire Sows

Yang, Lina,Bian, Gaorui,Su, Yong,Zhu, Weiyun Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.6

The objective of this study was to investigate differences in the faecal microbial composition among Lantang, Bama, Erhualian, Meishan, Xiaomeishan, Duroc, Landrace, and Yorkshire sows and to explore the possible link of the pig breed with the gut microbial community. Among the sows, the Meishan, Landrace, Duroc, and Yorkshire sows were from the same breeding farm with the same feed. Fresh faeces were collected from three sows of each purebred breed for microbiota analysis and volatile fatty acid (VFA) determination. Denaturing gradient gel electrophoresis (DGGE) analysis revealed that samples from Bama, Erhualian, and Xiaomeishan sows, which from different farms, were generally grouped in one cluster, with similarity higher than 67.2%, and those from Duroc, Landrace, and Yorkshire sows were grouped in another cluster. Principal component analysis of the DGGE profile showed that samples from the foreign breeds and the samples from the Chinese indigenous breeds were scattered in two different groups, irrespective of the farm origin. Faecal VFA concentrations were significantly affected by the pig breed. The proportion of acetate was higher in the Bama sows than in the other breeds. The real-time PCR analysis showed that 16S rRNA gene copies of total bacteria, Firmicutes and Bacteroidetes were significantly higher in the Bama sows compared to Xiaomeishan and Duroc sows. Both Meishan and Erhualian sows had higher numbers of total bacteria, Firmicutes, Bacteroidetes and sulphate-reducing bacteria as compared to Duroc sows. The results suggest that the pig breed affects the composition of gut microbiota. The microbial composition is different with different breeds, especially between overseas breeds (lean type) and Chinese breeds (relatively obese type).

The role of peroxiredoxin V in (-)-epigallocatechin 3-gallate-induced multiple myeloma cell death.

Ren, Lina,Yang, Hee-Young,Choi, Hoon-In,Chung, Kyoung-Jin,Yang, Ung,Lee, Il-Kwon,Kim, Hyeoung-Joon,Lee, Dong-Seok,Park, Byung-Ju,Lee, Tae-Hoon Pergamon Press 2011 Oncology Research Vol.19 No.8

<P>(-)-Epigallocatechin 3-gallate (EGCG) is a potent antioxidant polyphenol in green tea that acts as an anticancer agent via both direct and indirect pathways. Although the relationship between EGCG's anticancer effects and its antioxidant activity is not fully understood, it is known that EGCG stimulates production of reactive oxygen species (ROS), which induce oxidative stress leading to cell death. In IM9 multiple myeloma cells, EGCG acted in a dose- and time-dependent manner to induce apoptotic cell death. Among the antioxidant enzymes expressed in IM9 cells, levels of peroxiredoxin V (PrdxV) were selectively and significantly reduced by EGCG. Moreover, the ROS scavenger NAC completely inhibited EGCG-induced apoptosis and PrdxV reduction, while overexpression of PrdxV, but not a Prdx(VC48S) mutant, protected IM9 cells from EGCG-induced apoptosis. EGCG-induced reductions in cell viability and PrdxV levels were also observed in primary CD138+ multiple myeloma cells from patients. These results suggest that PrdxV is a key target via which EGCG mediates its anticancer effects.</P>

Preparations, application of polysaccharide–protein nanoparticles and their assembly at the oil–water interface

Hui Yang,Shengnan Wang,Lina Yang,He Liu 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.1

With the development of nanotechnology, nanoparticles have played an important role in pharmaceuticals, foods and materials, in particular, protein/polysaccharide based composite nanoparticles have received attention from researchers for safety and green production. This paper summarized in detail the preparation methods, applications of protein/polysaccharide nanoparticles (PPNPs) in recent years, especially the mechanism of stabilizing the oil–water interface. Currently, the polysaccharides applied are more traditional, such as chitosan, pectin and carboxymethyl cellulose, so there is still a lot of room for the development of raw materials that can be used to prepare PPNPs. Based on this, we also proposed three promising polysaccharides: seaweed polysaccharide, lycium barbarum polysaccharide and lactobacillus exopolysaccharides, describing their characteristics as well as their application prospects, this article can serve as a reference for interested researchers.

Osmanthus fragrans Flower Extract and Acteoside Protect Against d-Galactose-Induced Aging in an ICR Mouse Model

Lina Xiong,Shuqin Mao,Baiyi Lu,Jiajia Yang,Fei Zhou,Yinzhou Hu,Yirong Jiang,Canxi Shen,Yajing Zhao 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.1

Osmanthus fragrans flower extract (OFE) is an organic extract from O. fragrans flower, which exhibits neuroprotective, free radical scavenging, and antioxidant effects. Therefore, the protective effect of OFE and acteoside against aging was studied. An aging ICR mouse model was established by chronically administering d-galactose (250 mg/kg) for 8 weeks. d-galactose induced spatial learning and memory impairments that were successfully inhibited by OFE and acteoside, which could shorten escape latency, improve platform crossing times, and increase zone time. The antioxidant potential of OFE and acteoside in vivo was evaluated by estimating the following: activities of antioxidant enzymes, such as glutathione peroxidase and aging-related enzyme, particularly monoamine oxidase; contents of lipid peroxidation methane dicarboxylic aldehyde, advanced glycation end products, and 8-hydroxy-2'-deoxyguanosine (a DNA damage product); and levels of nuclear factor-erythroid 2-related factor 2. OFE and acteoside also inhibited d-galactose-induced neurological aging by suppressing the increase in glial fibrillary acidic protein and neurotrophin-3. Considering the dose-dependent protective effects of OFE and acteoside, we concluded that OFE, rich in acteoside, was a good source of natural antiaging compounds.

Insulin Resistance Is an Important Risk Factor for Cognitive Impairment in Elderly Patients with Primary Hypertension

Lina Ma,Yun Li,Ming Feng,Yuying Qian,Wei Yang,Jia Liu,Rui Han,Hong Zhu 연세대학교의과대학 2015 Yonsei medical journal Vol.56 No.1

Purpose: Insulin resistance plays a role in the development of dementia and hypertension. We investigated a possible relationship between cognitive impairment and insulin resistance in elderly Chinese patients with primary hypertension. Materialsand Methods: One hundred and thirty-two hypertensive elderly patients (>60 years) were enrolled in this study, and assigned into either the cognitive impairmentgroup (n=61) or the normal cognitive group (n=71). Gender, age, education,body mass index (BMI), waist hip ratio (WHR), total cholesterol (TC), triglyceride(TG), C-reactive protein (CRP), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), creatinine (Cr), fasting plasma glucose (FPG), fasting insulin (FINS), homeostasis model of assessment for insulin resistance index (HOMA-IR), systolic blood pressure, diastolic blood pressure, smoking history, atherosclerosis and the proportion of uncontrolled hypertensionwere compared between the two groups. Multi-factorial logistic regressionanalysis was performed. Results: No significant differences were found in gender, age, TC, CRP, HDL-C, LDL-C, Cr, BP, smoking history, atherosclerosis and the proportion of uncontrolled hypertension between the two groups. The cognitiveimpairment group had lower education levels, and higher BMI, WHR, TG, FPG, FINS, and HOMA-IR levels than the control group. Logistic regression analysis revealed the levels of education, BMI, WHR, and HOMA-IR as independentfactors that predict cognitive impairment in patients. Conclusion: Our study demonstrates that poor education and increased BMI, WHR, and HOMA-IR are independent risk factors for cognitive impairment in elderly patients with hypertension. Insulin resistance plays an important role in the development of cognitive impairment in primary elderly hypertensive patients.

Effect of Trichostatin A on CNE2 Nasopharyngeal Carcinoma Cells - Genome-wide DNA Methylation Alteration

Yang, Xiao-Li,Zhang, Cheng-Dong,Wu, Hua-Yu,Wu, Yong-Hu,Zhang, Yue-Ning,Qin, Meng-Bin,Wu, Hua,Liu, Xiao-Chun,Lina, Xing,Lu, Shao-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Trichostatin A (TSA) is a histone deacetylase (HDAC) inhibitor. We here investigated its effects on proliferation and apoptosis of the CNE2 carcinoma cell line, and attempted to establish genome-wide DNA methylation alteration due to differentially histone acetylation status. After cells were treated by TSA, the inhibitory rate of cell proliferation was examined with a CCK8 kit, and cell apoptosis was determined by flow cytometry. Compared to control, TSA inhibited CNE2 cell growth and induced apoptosis. Furthermore, TSA was found to induce genome-wide methylation alteration as assessed by genome-wide methylation array. Overall DNA methylation level of cells treated with TSA was higher than in controls. Function and pathway analysis revealed that many genes with methylation alteration were involved in key biological roles, such as apoptosis and cell proliferation. Three genes (DAP3, HSPB1 and CLDN) were independently confirmed by quantitative real-time PCR. Finally, we conclude that TSA inhibits CNE2 cell growth and induces apoptosis in vitro involving genome-wide DNA methylation alteration, so that it has promising application prospects in treatment of NPC in vivo. Although many unreported hypermethylated/hypomethylated genes should be further analyzed and validated, the pointers to new biomarkers and therapeutic strategies in the treatment of NPC should be stressed.

Regulation of Noncoding Transcriptome in Developing Photoreceptors by Rod Differentiation Factor NRL

Zelinger, Lina,Karakü,lah, Gö,khan,Chaitankar, Vijender,Kim, Jung-Woong,Yang, Hyun-Jin,Brooks, Matthew J.,Swaroop, Anand The Association for Research in Vision and Ophthal 2017 Investigative Ophthalmology & Visual Science Vol.58 No.11

<P><B>Purpose</B></P><P>Transcriptome analysis by next generation sequencing allows qualitative and quantitative profiling of expression patterns associated with development and disease. However, most transcribed sequences do not encode proteins, and little is known about the functional relevance of noncoding (nc) transcriptome in neuronal subtypes. The goal of this study was to perform a comprehensive analysis of long noncoding (lncRNAs) and antisense (asRNAs) RNAs expressed in mouse retinal photoreceptors.</P><P><B>Methods</B></P><P>Transcriptomic profiles were generated at six developmental time points from flow-sorted <I>Nrl</I>p-GFP (rods) and <I>Nrl</I>p-GFP;<I>Nrl</I><SUP>−/−</SUP> (S-cone like) mouse photoreceptors. Bioinformatic analysis was performed to identify novel noncoding transcripts and assess their regulation by rod differentiation factor neural retina leucine zipper (NRL). In situ hybridization (ISH) was used for validation and cellular localization.</P><P><B>Results</B></P><P>NcRNA profiles demonstrated dynamic yet specific expression signature and coexpression clusters during rod development. In addition to currently annotated 586 lncRNAs and 454 asRNAs, we identified 1037 lncRNAs and 243 asRNAs by de novo assembly. Of these, 119 lncRNAs showed altered expression in the absence of NRL and included NRL binding sites in their promoter/enhancer regions. ISH studies validated the expression of 24 lncRNAs (including 12 previously unannotated) and 4 asRNAs in photoreceptors. Coexpression analysis demonstrated 63 functional modules and 209 significant antisense-gene correlations, allowing us to predict possible role of these lncRNAs in rods.</P><P><B>Conclusions</B></P><P>Our studies reveal coregulation of coding and noncoding transcripts in rod photoreceptors by NRL and establish the framework for deciphering the function of ncRNAs during retinal development.</P>

An ANN-based Intelligent Spectrum Sensing Algorithm for Space-based Satellite Networks

Xiujian Yang,Lina Wang 한국인터넷정보학회 2023 KSII Transactions on Internet and Information Syst Vol.17 No.3

In Low Earth Orbit (LEO) satellite networks, satellites operate fast and the inter-satellite link change period is short. In order to sense the spectrum state in LEO satellite networks in real-time, a space-based satellite network intelligent spectrum sensing algorithm based on artificial neural network (ANN) is proposed, while Geosynchronous Earth Orbit (GEO) satellites are introduced to make fast and effective judgments on the spectrum state of LEO satellites by using their stronger arithmetic power. Firstly, the visibility constraints between LEO satellites and GEO satellites are analyzed to derive the inter-satellite link building matrix and complete the inter-satellite link situational awareness. Secondly, an ANN-based energy detection (ANN-ED) algorithm is proposed based on the traditional energy detection algorithm and artificial neural network. The ANN module is used to determine the spectrum state and optimize the traditional energy detection algorithm. GEO satellites are used to fuse the information sensed by LEO satellites and then give the spectrum decision, thereby realizing the inter-satellite spectrum state sensing. Finally, the sensing quality is evaluated by the analysis of sensing delay and sensing energy consumption. The simulation results show that our proposed algorithm has lower complexity, the sensing delay and sensing energy consumption compared with the traditional energy detection method.

SMAD4 Suppresses AURKA-Induced Metastatic Phenotypes via Degradation of AURKA in a TGFβ-Independent Manner

Jia, Lina,Lee, Hun Seok,Wu, Chun Fu,Kundu, Juthika,Park, Sang Gyu,Kim, Ryong Nam,Wang, Li-Hui,Erkin, Ö,zgü,r Cem,Choi, Jong-Sun,Chae, Seoung Wan,Yang, Ho Bin,Choi, Yoon-La,Shin, Young Kee American Association for Cancer Research 2014 Molecular Cancer Research Vol.12 No.12

<P>SMAD4 has been suggested to inhibit the activity of the WNT/β-catenin signaling pathway in cancer. However, the mechanism by which SMAD4 antagonizes WNT/β-catenin signaling in cancer remains largely unknown. Aurora A kinase (AURKA), which is frequently overexpressed in cancer, increases the transcriptional activity of β-catenin/T-cell factor (TCF) complex by stabilizing β-catenin through the inhibition of GSK-3β. Here, SMAD4 modulated AURKA in a TGFβ-independent manner. Overexpression of SMAD4 significantly suppressed AURKA function, including colony formation, migration, and invasion of cell lines. In addition, SMAD4 bound to AURKA induced degradation of AURKA by the proteasome. A luciferase activity assay revealed that the transcriptional activity of the β-catenin/TCF complex was elevated by AURKA, but decreased by SMAD4 overexpression. Moreover, target gene analysis showed that SMAD4 abrogated the AURKA-mediated increase of β-catenin target genes. However, this inhibitory effect of SMAD4 was abolished by overexpression of AURKA or silencing of AURKA in SMAD4-overexpressed cells. Meanwhile, the SMAD4-mediated repression of AURKA and β-catenin was independent of TGFβ signaling because blockage of TGFβR1 or restoration of TGFβ signaling did not prevent suppression of AURKA and β-catenin signaling by SMAD4. These results indicate that the tumor-suppressive function of SMAD4 is mediated by downregulation of β-catenin transcriptional activity via AURKA degradation in a TGFβ-independent manner.</P><P><B>Implications:</B> SMAD4 interacts with AURKA and antagonizes its tumor-promoting potential, thus demonstrating a novel mechanism of tumor suppression. <I>Mol Cancer Res; 12(12); 1779–95. ©2014 AACR</I>.</P>