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- 저자 : Zelinger, Lina (검색결과 2 건)
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Regulation of Noncoding Transcriptome in Developing Photoreceptors by Rod Differentiation Factor NRL
Zelinger, Lina,Karakü,lah, Gö,khan,Chaitankar, Vijender,Kim, Jung-Woong,Yang, Hyun-Jin,Brooks, Matthew J.,Swaroop, Anand The Association for Research in Vision and Ophthal 2017 Investigative Ophthalmology & Visual Science Vol.58 No.11
<P><B>Purpose</B></P><P>Transcriptome analysis by next generation sequencing allows qualitative and quantitative profiling of expression patterns associated with development and disease. However, most transcribed sequences do not encode proteins, and little is known about the functional relevance of noncoding (nc) transcriptome in neuronal subtypes. The goal of this study was to perform a comprehensive analysis of long noncoding (lncRNAs) and antisense (asRNAs) RNAs expressed in mouse retinal photoreceptors.</P><P><B>Methods</B></P><P>Transcriptomic profiles were generated at six developmental time points from flow-sorted <I>Nrl</I>p-GFP (rods) and <I>Nrl</I>p-GFP;<I>Nrl</I><SUP>−/−</SUP> (S-cone like) mouse photoreceptors. Bioinformatic analysis was performed to identify novel noncoding transcripts and assess their regulation by rod differentiation factor neural retina leucine zipper (NRL). In situ hybridization (ISH) was used for validation and cellular localization.</P><P><B>Results</B></P><P>NcRNA profiles demonstrated dynamic yet specific expression signature and coexpression clusters during rod development. In addition to currently annotated 586 lncRNAs and 454 asRNAs, we identified 1037 lncRNAs and 243 asRNAs by de novo assembly. Of these, 119 lncRNAs showed altered expression in the absence of NRL and included NRL binding sites in their promoter/enhancer regions. ISH studies validated the expression of 24 lncRNAs (including 12 previously unannotated) and 4 asRNAs in photoreceptors. Coexpression analysis demonstrated 63 functional modules and 209 significant antisense-gene correlations, allowing us to predict possible role of these lncRNAs in rods.</P><P><B>Conclusions</B></P><P>Our studies reveal coregulation of coding and noncoding transcripts in rod photoreceptors by NRL and establish the framework for deciphering the function of ncRNAs during retinal development.</P>
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Qian Hao,Furen Zhang,Zeling Liu,Lele Qin 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.4
Chemical industry is the fundamental strategic industry in national economy and one of pillar industries in China. A wealth of chemical industrial parks has been built one after another in many places, where lots of chemical enterprises are established. In the normal operation of those enterprises, their raw materials or products manufactured, transported and stored are usually featured by flammable, explosive or toxic properties, resulting in frequent occurrence of pollution and personal accidents and causing great threats to surrounding environment. This thesis realizes an integrated information management platform based on currently advanced cloud computing and IOT (The Internet of Things) technologies, so as for integrated management of chemical industrial parks. This thesis designs the architectural structure of said integrated information management platform, expounds the roles and realization approaches of the basic support platform and the business service platform, analyzes the principles and methods for realization of regionally coordinate emergency management operation mechanism based on the information management platform, that of IOT architecture technology and that of RFID and Wireless Sensor Network (WSN), and discusses that the standard unification of heterogeneous data under cloud computing is realized by means of multi-source data integration middleware. The said platform can accomplish the whole-process, real-time and dynamic follow-up and control of chemicals and hazardous materials and also provide effective service for users’ decision-making by mining and analyzing tremendous data of a chemical industrial park with Agent-middleware technology. The information management platform is capable of assessing the safety factors of chemical enterprises at peacetime, giving necessary warning before accidents and offering effective technical support for rescue via expert system, emergency plan system, monitoring system and positioning system. The realization of chemical industrial park integrated information management platform based on cloud computing and IOT technologies will greatly improve the management level of chemical industrial parks, intensify surveillance, achieve information resource sharing and lower the probability of accident occurrence.
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