454 Pyrosequencing Analysis on Microbial Diversity of an Expanded Granular Sludge Bed Reactor Treating High NaCl and Nitrate Concentration Wastewater

Runhua Liao,Yan Li,Zhu Wang,Yu Miao,Ke Shen,Peng Shi,Yan Ma,Wentao Li,Aimin Li 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.1

The treatment of high-salinity, high-nitratewastewater was investigated in a single expanded granularsludge bed reactor. Complete denitrification was achievedwhen nitrate concentration was as high as 6,000 mg N/Land the salinity of influent reached 11% NaCl at liquidup-flow velocity of 3.0 m/h, hydraulic retention time of,24 h and the C/N molar ratio of 2.0. Furthermore, 454-pyrosequencing technology was used to analyze archaeabacterial diversity under high salinity and high nitrateconditions. Results showed that the total number ofeffective sequences was 5749 consisting of 5678 bacterialsequences and 71 archaea sequences after denoising andfiltering out chimeras, which could be affiliated to 5phylogenetic groups, including Proteobacteria, Firmicutes,Euryarchaeota, Crenarchaeota and unclassified phylum. Although Proteobacteria was the dominant microbialpopulation, two archaea phylogenetic groups—Crenarchaeotaand Euryarchaeota were observed in this study.

Meta-analysis of Outcomes Compared between Robotic and Laparoscopic Gastrectomy for Gastric Cancer

Liao, Gui-Xiang,Xie, Guo-Zhu,Li, Rong,Zhao, Zhi-Hong,Sun, Quan-Quan,Du, Sha-Sha,Ren, Chen,Li, Guo-Xing,Deng, Hai-Jun,Yuan, Ya-Wei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

This meta-analysis was performed to evaluate and compare the outcomes of robotic gastrectomy (RG) and laparoscopic gastrectomy (LG) for treating gastric cancer. A systematic literature search was carried out using the PubMed database, Web of Knowledge, and the Cochrane Library database to obtain comparative studies assessing the safety and efficiency between RG and LG in May, 2013. Data of interest were analyzed by using of Review Manager version 5.2 software (Cochrane Collaboration). A fixed effects model or random effects model was applied according to heterogeneity. Seven papers reporting results that compared robotic gastrectomy with laparoscopic gastrectomy for gastric cancer were selected for this meta-analysis. Our metaanalysis included 2,235 patients with gastric cancer, of which 1,473 had undergone laparoscopic gastrectomy, and 762 had received robotic gastrectomy. Compared with laparoscopic gastrectomy, robotic gastrectomy was associated with longer operative time but less blood loss. There were no significant difference in terms of hospital stay, total postoperative complication rate, proximal margin, distal margin, numbers of harvested lymph nodes and mortality rate between robotic gastrectomy and laparoscopic gastrectomy. Our meta-analysis showed that robotic gastrectomy is a safe technique for treating gastric cancer that compares favorably with laparoscopic gastrectomy in short term outcomes. However, the long term outcomes between the two techniques need to be further examined.

Integrated proteomic and metabolomic analyses reveal significant changes in chloroplasts and mitochondria of pepper (Capsicum annuum L.) during Sclerotium rolfsii infection

Liao Hongdong,Wen Xiangyu,Deng Xuelei,Wu Yonghong,Xu Jianping,Li Xin,Zhou Shudong,Li Xuefeng,Zhu Chunhui,Luo Feng,Ma Yanqing,Zheng Jingyuan 한국미생물학회 2022 The journal of microbiology Vol.60 No.5

Infection by Sclerotium rolfsii will cause serious disease and lead to significant economic losses in chili pepper. In this study, the response of pepper during S. rolfsii infection was explored by electron microscopy, physiological determination and integrated proteome and metabolome analyses. Our results showed that the stomata of pepper stems were important portals for S. rolfsii infection. The plant cell morphology was significantly changed at the time of the fungal hyphae just contacting (T1) or surrounding (T2) the pepper. The chlorophyll, carotenoid, and MDA contents and the activities of POD, SOD, and CAT were markedly upregulated at T1 and T2. Approximately 4129 proteins and 823 metabolites were clearly identified in proteome and metabolome analyses, respectively. A change in 396 proteins and 54 metabolites in pepper stem tissues was observed at T1 compared with 438 proteins and 53 metabolites at T2. The proteins and metabolites related to photosynthesis and antioxidant systems in chloroplasts and mitochondria were disproportionally affected by S. rolfsii infection, impacting carbohydrate and amino acid metabolism. This study provided new insights into the response mechanism in pepper stems during S. rolfsii infection, which can guide future work on fungal disease resistance breeding in pepper.

Efficacy and Safety of Pulse Magnetic Therapy System in Insomnia Disorder: A Multicenter, Randomized, Double-Blind, Placebo-Controlled Trial

Liao Jiwu,Wang Sisi,Zhou Borong,Liang Wei,Ma Ping,Lin Min,Lin Weisen,Li Congrui,Zhang Xiaotao,Li Hongyao,Cui Yin,Hu Jiajia,Qin Yuanyi,Deng Yanhua,Fu Aibing,Zhu Tianhua,Zhang Shanlian,Qu Yunhong,Xing L 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.6

Objective This study’s objective is to assess the efficacy and safety of Pulsed Magnetic Therapy System (PMTS) in improving insomnia disorder.Methods Participants with insomnia disorder were randomly assigned to receive either PMTS or sham treatment for four weeks (n= 153; PMTS: 76, sham: 77). Primary outcomes are the Insomnia Severity Index (ISI) scores at week 0 (baseline), 1, 2, 3, 4 (treatment), and 5 (follow-up). Secondary outcomes are the Pittsburgh Sleep Quality Index at baseline and week 4, and weekly sleep diary-derived values for sleep latency, sleep efficiency, real sleep time, waking after sleep onset, and sleep duration.Results The ISI scores of the PMTS group and the sham group were 7.13±0.50, 11.07±0.51 at week 4, respectively. There was a significant group×time interaction for ISI (F3.214, 485.271=24.25, p<0.001, ηp 2=0.138). Only the PMTS group experienced continuous improvement throughout the study; in contrast, the sham group only experienced a modest improvement after the first week of therapy. At the end of the treatment and one week after it, the response of the PMTS group were 69.7% (95% confidence interval [CI]: 58.6%–79.0%), 75.0% (95% CI: 64.1%–83.4%), respectively, which were higher than the response of the sham group (p<0.001). For each of the secondary outcomes, similar group×time interactions were discovered. The effects of the treatment persisted for at least a week.Conclusion PMTS is safe and effective in improving insomnia disorders.

Will a Wiener Filter Decrease the Accuracy of HRTEM Displacement Measurements of Aperiodic Structures?

Gen Li,Dongsheng Song,Zhenyu Liao,Jing Zhu 한국물리학회 2019 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.74 No.12

Modern Cs-corrected high-resolution transmission electron microscopy (HRTEM) has pushed the resolution limit to sub-angstrom scale and has made possible the quantitative analyses of local aperiodic atomic structures. After images have been obtained, a Wiener filter is often used to improve the signal-to-noise ratio, especially for those samples containing both crystal and large amorphous components. However, a Wiener filter may introduce distortions in the original experimental images. From this point of view, having a fundamental understanding of the effect of a Wiener filter on the accuracy of atomic displacement measurements in aperiodic structures is important. In this work, we first review the principle of the Wiener filter and theoretically discuss the origin of the distortions induced in aperiodic structures by using a Wiener filter. Then, using hypothetical experimental systems that contains both aperiodic crystal structures and amorphous layers, we carried out synthetic experiments to quantitatively estimate the effect of the Wiener filter on the measurements of aperiodic displacements. Compared with the values for a non-filtered image, the signal-to-noise ratio was significantly improved, and the accuracy of the displacement measurement was not decreased when proper Wiener filter parameters were used. Such results are of great importance for the processing of HRTEM images.

Development of covalent inhibitors that can overcome resistance to first-generation FGFR kinase inhibitors

Tan, Li,Wang, Jun,Tanizaki, Junko,Huang, Zhifeng,Aref, Amir R.,Rusan, Maria,Zhu, Su-Jie,Zhang, Yiyun,Ercan, Dalia,Liao, Rachel G.,Capelletti, Marzia,Zhou, Wenjun,Hur, Wooyoung,Kim, NamDoo,Sim, Taebo,G National Academy of Sciences 2014 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.111 No.45

<P><B>Significance</B></P><P>Inhibitors of the FGF receptors (FGFRs) are currently under clinical investigation for the treatment of various cancers. All currently approved kinase inhibitors eventually are rendered useless by the emergence of drug-resistant tumors. We used structure-based drug design to develop the first, to our knowledge, selective, next-generation covalent FGFR inhibitors that can overcome the most common form of kinase inhibitor resistance, the mutation of the so-called “gatekeeper” residue located in the ATP-binding pocket. We also describe a novel kinase inhibitor design strategy that uses a single electrophile to target covalently cysteines that are located in different positions within the ATP-binding pocket. These results have important implications for the design of covalent FGFR inhibitors that can overcome clinical resistance.</P><P>The human FGF receptors (FGFRs) play critical roles in various human cancers, and several FGFR inhibitors are currently under clinical investigation. Resistance usually results from selection for mutant kinases that are impervious to the action of the drug or from up-regulation of compensatory signaling pathways. Preclinical studies have demonstrated that resistance to FGFR inhibitors can be acquired through mutations in the FGFR gatekeeper residue, as clinically observed for FGFR4 in embryonal rhabdomyosarcoma and neuroendocrine breast carcinomas. Here we report on the use of a structure-based drug design to develop two selective, next-generation covalent FGFR inhibitors, the FGFR irreversible inhibitors 2 (FIIN-2) and 3 (FIIN-3). To our knowledge, FIIN-2 and FIIN-3 are the first inhibitors that can potently inhibit the proliferation of cells dependent upon the gatekeeper mutants of FGFR1 or FGFR2, which confer resistance to first-generation clinical FGFR inhibitors such as NVP-BGJ398 and AZD4547. Because of the conformational flexibility of the reactive acrylamide substituent, FIIN-3 has the unprecedented ability to inhibit both the EGF receptor (EGFR) and FGFR covalently by targeting two distinct cysteine residues. We report the cocrystal structure of FGFR4 with FIIN-2, which unexpectedly exhibits a “DFG-out” covalent binding mode. The structural basis for dual FGFR and EGFR targeting by FIIN3 also is illustrated by crystal structures of FIIN-3 bound with FGFR4 V550L and EGFR L858R. These results have important implications for the design of covalent FGFR inhibitors that can overcome clinical resistance and provide the first example, to our knowledge, of a kinase inhibitor that covalently targets cysteines located in different positions within the ATP-binding pocket.</P>

Structural Characterization of Microwave-Assisted Solution-Synthesized Strontium-Substituted Hydroxyapatite

Jianguo Liao,Yanqun Li,Xiali Guan,Jingxian Liu,Yongxiang Zhang,Yufen Xie,Zhengpeng Yang,Xingze Duan,Aiguo Zhou,Jiangnan Zhu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.10

Hydroxyapatite and strontium-substituted hydroxyapatite nanoparticles (n-HA and Sr-HA) were prepared by microwave-assisted solution synthesis with aqueous solutions of various Sr/(Sr+Ca) molar ratios ranging from 0% to 15%. The structural properties of the hydroxyapatite powders were investigated by X-ray powder diffraction, Fourier transform infrared spectroscopy and Raman spectra, field emission scanning electron microscopy coupled with energy dispersive X-ray spectrometry and transmission electron microscopy. The results confirmed that strontium ions had been incorporated into the hydroxyapatite lattice. The synthetic n-HA and Sr-HA nanocrystalline consisted of hydroxyapatite crystalline phase with hexagonal structure, and the particle size was 30–40 x 60–70 nm and 40–50 x 70–80 nm, respectively. The calcined HA particle size ranged from about 120 nm to 150 nm, the calcined Sr-HA products were composed of spherical aggregates with a size of about 70–100 nm. The incorporation of Sr ions lead to the formation of vacancies in the crystal structure of the HA. The results indicated that the strontium substitution did not change the crystal structures. More Sr resulted in less calcined crystallites and formed agglomerates owing to the size effect.

Mediatory role of K, Cu and Mo over Ru/SiO2 catalysts for glycerol hydrogenolysis

Xiaoyuan Liao,Kewen Li,Xiaomin Xiang,Sheng-Guang Wang,Xichun She,Yulei Zhu,Yongwang Li 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.2

SiO2 supported ruthenium catalysts with and without modifiers were prepared, characterized and tested for glycerol hydrogenation. Addition of K, Cu and Mo affects the reducibility and acidity of the Ru/SiO2catalyst. Characterization data shows that Cu and Mo-modified Ru/SiO2 have stronger acidity. On the contrary, K element on a passive effect on the acidity of Ru based catalyst had been observed. A comparison with the pure Ru/SiO2 indicates the Cu-promoted specimen has better selective to the desired products, acetol, 1,2-propanediol and ethyl glycol, although the reactivity is slightly lower.

Phase II trial of VEGFR2 inhibitor apatinib for metastatic sarcoma: focus on efficacy and safety

Zhichao Liao,Feng Li,Chao Zhang,Lei Zhu,Yehui Shi,Gang Zhao,Xu Bai,Shafat Hassan,Xinyue Liu,Ting Li,Peipei Xing,Jun Zhao,Jin Zhang,Ruwei Xing,Sheng Teng,Yun Yang,Kexin Chen,Jilong Yang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Apatinib (YN968D1) is a novel tyrosine kinase inhibitor targeting vascular endothelial growth factor receptor 2 (VEGFR- 2). We conducted a single-arm, nonrandomized phase II study (NCT03121846) to assess the efficacy and safety of apatinib in patients with stage IV sarcoma. We recruited 64 patients with stage IV sarcoma who had failed chemotherapy. The primary endpoint was progression-free survival (PFS), and the secondary endpoints were progression-free survival rate (PFR), objective response rate (ORR), and disease control rate (DCR) at week 12. Treatment-related adverse effects (AEs) were evaluated. Fifty-nine patients were assessed for efficacy and 64 patients for AEs. The median PFS was 7.93 months. At 12 weeks, the PFR was 74%, the ORR was 16.95% (10/59), and the DCR was 86.44% (51/59). The final ORR was 15.25% (9/59) and the DCR was 57.63% (34/59). Notably, 22 patients (34.38%) who developed hypertension, hand-foot-skin reaction, or proteinuria had significantly longer OS than those without these AEs (18.20 vs. 10.73 months; P = 0.002). We conclude that apatinib is effective and well tolerated in patients with advanced sarcoma. The development of hypertension, hand-foot-skin reaction, or proteinuria may indicate a favorable prognosis, representing a novel finding in sarcoma patients.

Synthesis of BiVO₄@C Core-Shell Structure on Reduced Graphene Oxide with Enhanced Visible-Light Photocatalytic Activity

Sun, Zhihua,Li, Chenzhe,Zhu, Shenmin,Cho, Maenghyo,Chen, Zhixin,Cho, Kyeongjae,Liao, Yongliang,Yin, Chao,Zhang, Di Wiley (John WileySons) 2015 CHEM SUS CHEM Vol.8 No.16