Oriental herbs as a source of novel anti-androgen and prostate cancer chemopreventive agents

LÜ,, Junxuan,KIM, Sung- Hoon,JIANG, Cheng,LEE, HyoJeong,GUO, Junming Nature Publishing Group 2007 Acta Pharmacologica Sinica Vol.28 No.9

<P>Androgen and androgen receptor (AR) signaling are crucial for the genesis of prostate cancer (PCa), which can often develop into androgen-ligand-independent diseases that are lethal to the patients. Recent studies show that even these hormone-refractory PCa require ligand-independent AR signaling for survival. As current chemotherapy is largely ineffective for PCa and has serious toxic sideeffects, we have initiated a collaborative effort to identify and develop novel, safe and naturally occurring agents that target AR signaling from Oriental medicinal herbs for the chemoprevention and treatment of PCa. We highlight our discovery of decursin from an Oriental formula containing Korean Angelica gigas Nakai (Dang Gui) root as a novel anti-androgen/AR agent. We have identified the following mechanisms to account for the specific anti-AR actions: rapid block of AR nuclear translocation, inhibition of binding of 5alpha-dihydrotestesterone to AR and increased proteasomal degradation of AR protein. Furthermore, decursin lacks the agonist activity of the 'pure' anti-androgen bicalutamide and is more potent than bicalutamide in inducing PCa apoptosis. Structure-activity analyses reveal a critical requirement of the side-chain on decursin or its structural isomer decursinol angelate for anti-AR, cell cycle arrest and proapoptotic activities. This work demonstrates the feasibility of using activity-guided fractionation in cell culture assays combined with mechanistic studies to identify novel anti-androgen/ AR agents from complex herbal mixtures.</P>

民主政治與中國傳統文化

江炳倫 서강대학교 동아연구소 1987 東亞 硏究 Vol.- No.12

여대생의 먹방 시청과 생활 스트레스, 음식중독 증상에 대한 연구

김대희,김영서,박채연,백정희,윤예원,장정원,전혜린,차윤정,Jiang Han,양숙자 이화여자대학교간호학회 2020 이화간호학회지 Vol.- No.54

Purpose: This study aimed to know whether there is a comparison of food video watching, life stress, food addiction symptom for female college students. Methods: A quantitative research is used. A total of 162 people were performed with questionnaire which was conducted from September 16, 2019 to October 2, 2019. The study sample was female college students of one university in Seoul. This study used general characteristics, life stress tool, and food addiction symptoms tool as a research tool. In dependent T-test, ANOVA, and pearson’s correlation was used for this study. Result: The food video watching time and the food addiction symptoms showed a significant positive of correlation (r=.230, p=.003). Also, life stress and food addiction symptoms were significantly correlated (r=.329, p<.001). However, food video watching time and life stress did not show statistically significant correlation (r=.062, p=.430). Conclusion: Based on the study results, food video watching time and life stress are highly correlated with food addiction symptoms. The findings of this study could be the basis for later research on nursing intervention in the life stress and food addiction symptoms among female college students.

Effects of Alfalfa Meal on Growth Performance and Gastrointestinal Tract Development of Growing Ducks

Jiang, J.F.,Song, X.M.,Huang, X.,Zhou, W.D.,Wu, J.L.,Zhu, Z.G.,Zheng, H.C.,Jiang, Y.Q. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.10

A study was conducted to evaluate effects of alfalfa meal on growth performance and gastrointestinal tract development of growing layer ducks to provide evidence for application of alfalfa meal in the duck industry. Two hundred and fifty-six healthy Shaoxing 7-wk old growing layer ducks were selected and randomly allocated to 1 of 4 dietary treatments based on corn and soybean meal and containing 0, 3, 6, and 9% of alfalfa meal for 8 wks. Each treatment consisted of 4 replicates of 16 ducks each. Briefly, birds were raised in separate compartments, and each compartment consisted of three parts: indoor floor house, adjacent open area and a connecting water area. The results showed: i) Growing ducks fed alfalfa meal diet were not significantly different in average daily gain, feed intake and gain-to-feed ratio from those fed no alfalfa diet (p>0.05). ii) Alfalfa meal increased the ratio crop, gizzard to live weight, caecum to live weight, the caecum index of growing ducks (p<0.05). iii) Villus height in duodenum and jejunum of growing ducks increased significantly with the increase of alfalfa meal levels (p<0.05). Crypt depth in duodenum and jejunum of growing ducks decreased significantly with the increase of alfalfa meal levels (p<0.05). This experiment showed that feeding of alfalfa meal to growing layer ducks could improve gastrointestinal tract growth and small intestinal morphology without effect on performance. This experiment provides evidence that alfalfa meal is a very valuable feedstuff for growing layer ducks.

Detection of 881^A→881^G Mutation in Tyrosinase Gene and Associations with the Black Ear Coat Color in Rabbits

Jiang, Y.L.,Fan, X.Z.,Lu, Z.X.,Tang, H.,Xu, J.-Q.,Du, L.-X. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.10

The tyrosinase gene was selected as a candidate for uncovering genetic mechanism causing 'black ear' coat color in rabbits. A PCR-SSCP detection method was established for the $881^A{\rightarrow}881^G$ mutation located in the central region of the tyrosinase gene between the CuA and CuB binding region signatures, and this was confirmed by sequencing and alignment. Fully consistent associations between the SNP and 'black ear' coat color were observed by analysis in a "black ear" pedigree and on 61 unrelated individuals. This SNP can serve as a molecular marker for use in "back ear" wool rabbit breeding.

Genotypexenvironment interactions for chilling tolerance of rice recombinant inbred lines under different low temperature environments

Jiang, W.,Lee, J.,Chu, S.H.,Ham, T.H.,Woo, M.O.,Cho, Y.I.,Chin, J.H.,Han, L.,Xuan, Y.,Yuan, D.,Xu, F.,Dai, L.,Yea, J.D.,Koh, H.J. Elsevier Scientific Pub. Co 2010 Field crops research Vol.117 No.2

Chilling injury is one of the major environmental stresses in rice cultivation in high-latitude and high-altitude regions. In this study, we cultivated a set of recombinant inbred lines (RILs) derived from Milyang 23 (indica)/Tong 88-7 (japonica) crosses in Kunming (high-altitude location), Yanji (high-latitude location), Chuncheon (cold water irrigation), and Suwon (normal) to evaluate the genotypexenvironment (GxE) interactions for chilling tolerance. RILs were the most severely damaged under the natural chilling air temperatures in Kunming. Significant GxE interactions in all measured agronomic traits were detected, and thus, the additive main effects and multiplicative interaction (AMMI) statistical model was applied to dissect the GxE interactions. The biplots of grand mean and IPCA1 (interaction principal component axes) of chilling-related traits accounted for most of the total treatment sums of squares. The IPCA scores of spikelet fertility and phenotypic acceptability were relatively smaller in Chuncheon than in Yanji and Kunming, implying that the screening for chilling tolerance with cold water irrigation in Chuncheon was more stable, whereas the Yanji and Kunming plantings were more sensitive to GxE interactions for chilling tolerance. These results demonstrate that multi-locational screening should be the best strategy for developing widely adaptable chilling-tolerant varieties in rice.

Magnetic Flux Transport at the Solar Surface

Jiang, J.,Hathaway, D. H.,Cameron, R. H.,Solanki, S. K.,Gizon, L.,Upton, L. Springer Science + Business Media 2014 Space science reviews Vol.186 No.1

Associations of T→A Mutation in the Promoter Region of Myostatin Gene with Birth Weight in Yorkshire Pigs

Jiang, Y-L,Li, N,Fan, X-Z,Xiao, L-R,Xiang, R-L,Hu, X-X,Du, L-X,Wu, C-X Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.11

A T$\longrightarrow$A mutation in the promoter region of porcine myostatin (MSTN) gene has been identified in our previous work. This study analyzed the associations of the myostatin genotypes (TT, TA) caused by this mutation with birth weigh in Yorkshire pigs. Data from 211 unrelated individuals were collected three times from one breeding farm. Detections of the mutation were carried out by PCR-RFLPs approach. The effects of MSTN genotypes (TT and TA) on birth weight were compared by least square means. The results showed that for birth weight of Yorkshire pigs, individuals with TA genotype were significantly higher than those with TT genotype (p<0.05), and the birth weight for pigs with TA genotype were 1.37 kg in average but only 1.25 kg for pigs with TT genotype, indicating a positive effect of birth weight for A allele.

Galactosylated chitosan-graft-polyethylenimine as a gene carrier for hepatocyte targeting

Jiang, H-L,Kwon, J-T,Kim, Y-K,Kim, E-M,Arote, R,Jeong, H-J,Nah, J-W,Choi, Y-J,Akaike, T,Cho, M-H,Cho, C-S Nature Publishing Group 2007 Gene Therapy Vol.14 No.19

Chitosans have been proposed as alternative, biocompatible cationic polymers for nonviral gene delivery. However, the low transfection efficiency and low specificity of chitosan need to be addressed before clinical application. We prepared galactosylated chitosan-graft-polyethylenimine (GC-g-PEI) copolymer by an imine reaction between periodate-oxidized GC and low-molecular-weight PEI. The molecular weight and composition were characterized using gel permeation chromatography column with multi-angle laser scattering and <SUP>1</SUP>H nuclear magnetic resonance, respectively. The copolymer was complexed with plasmid DNA in various copolymer/DNA (N/P) charge ratios, and the complexes were characterized. GC-g-PEI showed good DNA-binding ability and superior protection of DNA from nuclease attack and had low cytotoxicity compared to PEI 25K. GC-g-PEI/DNA complexes showed higher transfection efficiency than PEI 25K in both HepG2 and HeLa cell lines. Transfection efficiency into HepG2, which has asialoglycoprotein receptors, was higher than that into HeLa, which does not. GC-g-PEI/DNA complexes also transfected liver cells in vivo after intraperitoneal (i.p.) administration more effectively than PEI 25K. These results suggest that GC-g-PEI can be used in gene therapy to improve transfection efficiency and hepatocyte specificity in vitro and in vivo.Gene Therapy (2007) 14, 1389–1398; doi:10.1038/sj.gt.3302997; published online 19 July 2007

Low-voltage-drive and high output current ZnO thin-film transistors with sputtering SiO2 as gate insulator

L. Zhang,J. Li,X.W. Zhang,D.B. Yu,H.P. Lin Khizar-ul-Haq,X.Y. Jiang,Z.L. Zhang 한국물리학회 2010 Current Applied Physics Vol.10 No.5

Low-voltage-drive ZnO thin-film transistors (TFTs) with room-temperature radio frequency magnetron sputtering SiO2 as the gate insulator were fabricated successfully on the glass substrate. The ZnO-TFT operates in the enhancement mode with a threshold voltage of 4.2 V, a field effect mobility of 11.2 ㎠/V s, an on/off ratio of 3.1 × 106 and a subthreshold swing of 0.61 V/dec. The drain current can reach to 1 mA while the gate voltage is only of 12 V and drain voltage of 8 V. The C–V characteristics of a MOS capacitor with the structure of ITO/SiO2/ZnO/Al was investigated. The carrier concentration ND in the ZnO active layer was determined, the calculated ND is 1.81 × 1016 cm−3, which is the typical value of undoped ZnO film used as the channel layer for ZnO-TFT devices. The experiment results show that SiO2 film is a promising insulator for the low voltage and high drive capability oxide TFTs.