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문철웅,정종훈,박천국,이승일,배학연,장경식,김만우,정춘해,홍순표,이병래,김호중 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1
Renal ischemia is one of the most common causes of acute renal failure. Four factors related to the pathogenesis of acute renal failure are vasoconstriction, decreased glomerular filtration rate, tubular back leak of filtrate, and intratubular obstruction. The cellular response to renal ischemic insults include decreased content of adenosine trihosphate, lipid peroxidation induced membrane degradation, alteration in cellular pH, and calcium or phospholipase induced mitochondrial dysfunction. Much attention has been given to the role of increased cellular calcium as a pathogenetic contributor to cell injury during ischemia. Author studied the protective effects of calcium antagonists on cellular injury during renal ischemia in rat. To investigate the protective role of these agents, author measured the amount of malondialdehyde(MDA) and the enzyme activities of free radical scarvengers-superoxide dismutase(SOD), catalase and glutathione peroxidase from non-pretreated group and calcium antagonists pretreated groups in control, ischemia and reflow subgroups. The results were summerized as follows: 1) The amount of MDA in non-pretreated group was higher in the reflow compared with the control(<p<0.01). But, in all pretreated groups, there was no statistically difference in the amount of MDA. 2) The SOD activity in non-pretreated group was lower in both the ischemia and the reflow compared with the control (P<0.05). But, in both verapamil and trifluoperazine-pretreated groups, there was no statistically difference in the SOD activity. 3) Both catalase and glutathione peroxidase activities in non-pretreated group were lower in both the ischemia and the reflow compared with the control (P<0.05). But in all pretreated groups, there was no statically difference in both catalase and glutathione peroxidase activities. These results suggest that free radical mediated ischemic injury by renal artery clamp in rat can be protected by intraperitoneal pretreatment with calcium antagonists. As trifluoperazine has a protective effect in renal ischemia, the calcium activated calmodulin dependent enzyme may play a role in renal ischemic injury.
Pyo, Kyoung-Ho,Lee, You-Won,Lee, Sang-Hoon,Xin, Chun-Feng,Shin, Ji-Hun,Shin, Eun Hee NATURAL PRODUCT COMMUNICATIONS 2017 Natural product communications Vol.12 No.1
<P>The present study investigated the effect of peanut sprout extract (PSE) as a natural resveratrol supplement on chronic bacterial prostatitis (CBP) and estradiol-induced benign prostatic hyperplasia (BPH). PSE contained a high level of resveratrol (148.51 +/- 3.05 mu g/g), and was tested on the mouse models of CBP (induced by Escherichia colt 292 infection) and BPH (induced by treatment with beta-estradiol and dihydrotestosterone). PSE toxicity was assessed on the basis of changes in body weight, alanine aminotransferase activity (an indicator of hepatotoxicity), and expression of the kidney injury marker KIM-1. The effects of PSE on the histopathology of prostate tissue, the proportion of neutrophils, and immune cell profiles in the blood and spleen were examined. PSE administration did not result in any toxicity but reduced the bacterial burden and histopathological changes in the prostate. In addition, lymphocytes (CD4(+), CD8(+), and CD19(+)) in the spleen were significantly increased after PSE administration in CBP mice, suggesting immune enhancement. PSE treatment of bone marrow derived macrophages increased the expression of CD40, which is related to the pro-inflammatory function and host defense against pathogens. It is concluded that PSE would be a good supplement for the mitigation of prostate hyperplasia and prostatitis.</P>
Pyo, Kyoung Ho,Kim, Jae Hwan,Lee, Ji-Min,Kim, Sung Eun,Cho, Jae Seok,Lim, Sun Min,Cho, Byoung Chul Elsevier 2019 Lung cancer Vol.127 No.-
<P><B>Abstract</B></P> <P><B>Background</B></P> <P>With the advance of immunotherapy, treatment of non-small-cell lung cancer (NSCLC) has revolutionized by having anti-PD-1 therapy in front-line setting. In this era of cancer immunotherapy, humanized mouse models which recapitulate human immune system, are needed for predicting immunotherapy response in patients. We established a Hu-PBL-NSG mouse model which can be used as a preclinical testing platform for assessing efficacy of different immunotherapeutic agents.</P> <P><B>Materials and methods</B></P> <P>Hu-PBL-NSG mouse model was established by engrafting human peripheral blood mononuclear cells (PBMCs) into NOD/scid/IL-2Rγ<SUP>−/−</SUP> (NSG) mice. Cytokine array was performed to assess serological similarity between patient and the Hu-PBL-NSG mouse, and microscopic immune cell infiltration was observed in various organs mouse model. Human anti-PD-1 therapy was treated for assessing drug efficacy in patient-derived tumor.</P> <P><B>Results</B></P> <P>hCD3<SUP>+</SUP>hCD45<SUP>+</SUP> T-cells and antigen presenting cells (dendritic cells, macrophages, and MDSC) increased in the serum of Hu-PBL-NSG mouse 24 h after the transfusion of human PBMCs, and CD3 + T cells were observed in lung, liver, kidney, spleen sections. Cytokine arrays of human and Hu-PBL-NSG mouse revealed high similarity of Th1, Th2, Th17-related cytokines. A tumor xenograft was engrafted from an <I>EML4-ALK</I> patient, and Hu-PBL-NSG mouse was sacrificed for histological analyses. hCD3<SUP>+</SUP> T cells were infiltrated within the tumor, and CD11c + cells, which represent antigen-presenting capability, were seen in spleen, lung, liver and kidney. When anti-PD-1 Ab was treated intraperitoneally, xenograft tumor showed significant reduction in volume after day 6, and increased expression of immune response-related genes on microarray analysis in the tumor. Mostly IFN-gamma and its related gene sets were significantly changed (FDR < 0.25, GSEA).</P> <P><B>Conclusion</B></P> <P>Hu-PBL-NSG mouse model which highly resembles human immune system was successfully established. This model could be a strong preclinical model for testing efficacy of immunotherapeutic agents, and also for pursuing novel immunotherapy treatment strategies in advanced NSCLC.</P> <P><B>Highlights</B></P> <P>Hu-PBL-NSG mouse model can be used for assessing efficacy of immunotherapeutic agents.</P> <P>Hu-PBL-NSG mouse model highly recapitulates human immune system.</P> <P>Efficacy of anti-PD-1 Ab was observed in Hu-PBL-NSG mouse model.</P>
Kyoung-Ho Pyo,Eun-Young Kang,Bong-Kwang Jung,Jung-Ho Moon,Jong-Yil Chai,Eun-Hee Shin 대한기생충학열대의학회 2012 The Korean Journal of Parasitology Vol.50 No.1
Neodiplostomum seoulense (Digenea: Neodiplostomidae) is an intestinal trematode that can cause severe mucosal pathology in the small intestines of mice and even mortality of the infected mice within 28 days after infection. We observed neuronal growth associated protein-43 (GAP-43) expression in the myenteric plexus of the small intestinal wall of N. seoulense-infected mice until day 35 post-infection (PI). BALB/c mice were infected with 200 or 500 N. seoulense metacercariae isolated from naturally infected snakes and were killed every 7 days for immunohistochemical demonstration of GAP-43 in the small intestines. N. seoulense-infected mice showed remarkable dilatation of intestinal loops compared with control mice through days 7-28 PI. Conversely, GAP-43 expression in the mucosal myenteric plexus was markedly (P<0.05) reduced in the small intestines of N. seoulense-infected mice during days 7-28 PI and was slightly normalized at day 35 PI. From this study, it is evident that neuronal damage occurs in the intestinal mucosa of N. seoulense-infected mice. However, the correlation between intestinal pathology, including the loop dilatation, and depressed GAP-43 expression remains to be elucidated.
Kyoung-Ho Pyo,Bong-Kwang Jung,Jong-Yil Chai,Eun-Hee Shin 대한기생충학열대의학회 2010 The Korean Journal of Parasitology Vol.48 No.2
The anti-tumorigenic effects of Toxoplasma gondii (RH) antigens were studied in a murine sarcoma-180 tumor model. To determine the anti-tumor effects, the reduction in tumor size and expression of CD31 (an angiogenesis marker in the tumor tissue) were examined after injection of BALB/c mice with T. gondii lysate antigen (TLA) or formalin-fixed, proliferation-inhibited, T. gondii tachyzoites. Tumors were successfully produced by an intradermal injection of sarcoma-180 cells with plain Matrigel in the mid-backs of mice. After injection with TLA or formalin-fixed T. gondii tachyzoites, the increase in tumor size and weight nearly stopped while tumor growth continued in control mice that were injected with PBS. CD31 expression in TLA-treated or formalin-fixed T. gondii-injected mice was lower than the control mice. Accordingly, the present study shows that the treatment of mice with formalin-fixed T. gondii or TLA in the murine sarcoma-180 tumor model results in a decrease of both tumor size and CD31 expression.
영구자석 삽입형 Claw Pole Machine 의 회전자 최적 설계
임호경(Ho-Kyoung Lim),이상호(Sang-Ho Lee),김성일(Sung-Il Kim),홍정표(Jung-Pyo Hong) 대한전기학회 2010 대한전기학회 학술대회 논문집 Vol.2010 No.7
When permanent magnet is embedded in the rotor of claw pole machine, the magnetic saturation of rotor core is largely reduced. As a result of the reduction of magnetic saturation, reluctance is reduced. Thus, larger Back-EMF is obtained due to the increase of flux linkage. In this paper, C-core which is the simplified motor is used to define the phenomena. The merit to attach permanent magnet in the rotor of claw pole machine is confirmed through 3D-FEA. Finally, permanet magnet size is optimized to obtain the maximum Back-EMF increase.