A Comparative Study of Phenolic Antioxidant Activity and Flavonoid Biosynthesis-Related Gene Expression Between Summer and Winter Strawberry Cultivars : Polyphenolic content and antioxidant…

Park, Doori,Park, Yeri,Lee, Young Hun,Choi, Ik-Young,Park, Kyong Cheul,Park, Sang Un,Kim, Byung Sup,Yeoung, Young Rog,Park, Nam Il Wiley Blackwell (Blackwell Publishing) 2017 Journal of food science Vol.82 No.2

Review : Why Genes are in Pieces? A Genomics Perspective

Kyong Cheul Park,Soon Jae Kwon,Nam Soo Kim 한국유전학회 2008 Genes & Genomics Vol.30 No.5

Three decades after their discovery, both the biological function and evolutionary origin of introns still remain topics of considerable debate. Sequencing of complete genomes continues to provide key genetic insights into the elusive introns. Although supportive evidence exists both for an `introns-early` as well as an `introns-late` model of origin, neither theory is sufficient to account for the presence of both ancient and new introns in current eukaryotic genes; instead, a synthetic theory merging the two models may be valid. Insights into the biological role of introns are also available from genomics and experimental data sets. Introns regulate gene expression by means of a nonsense-mediated decay of mRNAs. Genome diversity generated by recombination between introns of paralogs, and proteome diversity generated by alternative intron splicing, would have been beneficial for the fitness of organisms. In gene families, intron gain or loss followed by gene duplication would have contributed to gene family divergence, and subsequently to species diversity and differentiation.

프락토올리고당과 이소말토올리고당을 첨가한 오미자청의 품질 특성 연구

박예리 ( Yeri Park ),최혁진 ( Hyeok-jin Choi ),최선강 ( Seon-kang Choi ),박경철 ( Kyong-cheul Park ),박남일 ( Nam Il Park ) 강원대학교 농업생명과학연구원 2022 강원 농업생명환경연구 Vol.34 No.0

본 연구에서는 오미자청 제조에 있어 설탕을 대체할 수 있는 당류 저감 소재를 사용하여 오미자청을 제조하고자 하였다. 오미자와 설탕, 프락토올리고당, 그리고 이소말토올리고당을 함량과 혼합 비율에 따라 다양한 청을 제조하였으며, 제조 후 저장 기간을 2주 간격으로 두어 생리활성을 평가하였다. 오미자청의 품질 특성을 분석한 결과 당도, 산도 및 당산비는 저장 기간에 따라 삼투압에 의해 오미자의 과실 내 수분이 용출되어 점차 감소하는 경향이 나타났으나, 당원에 따른 유의적인 차이는 나타나지 않았다. 총 페놀 함량은 설탕과 프락토올리고당을 혼합하여 제조한 오미자청 S7이 가장 높았으며, 저장 기간이 증가할수록 두 가지 이상의 올리고당을 혼합한 오미자청 S7-S10에서 높은 수치를 나타냈다. 총 플라보노이드 함량은 설탕으로 제조한 오미자청 S1에서 가장 높았으며, 그 다음으로 프락토올리고당으로 제조한 오미자청 S2가 높은 함량을 나타냈다. DPPH 라디칼 소거능은 설탕과 프락토올리고당을 혼합하여 제조한 오미자청 S7 처리구가 가장 높았고, 이소말토올리고당으로 제조한 오미자청 S3 처리구가 가장 낮았으나, ABTS 라디칼 소거능은 S3가 가장 높은 수치를 나타냈다. 마지막으로 α-glucosidase 저해활성은 설탕으로 제조한 오미자청 S1이 가장 저해 활성이 나타났지만, 낮은 pH에 대한 안전성을 나타내며 체내효소에 의해 흡수가 되지 않는 이소말토올리고당을 사용한 오미자청 S6이 당류 저감에 있어서 가장 실용적인 활용방안으로 판단된다. The purpose of this study is to prepare Omija cheong using sugar reducing materials such as fructooligosaccharide and isomaltooligosaccharide as a sucrose substitute. Sugar content, acidity, and sugar acid ratio were measured to evaluate the quality of the prepared syrup. Antioxidant activity, total phenol content, and total flavonoid content were measured to evaluate the physiological activity of the prepared Omija cheong. Acidity and sugar ratio tended to decrease gradually due to the eluting of water from the omija by osmotic pressure depending on the storage period, but there was no significant difference according to the sugar source. The total phenol content was highest in Omija cheong S7, prepared by mixing sugar and fructooligosaccharide; in S7-S10 containing two or more sweetners, the longer the storage period, the higher the total phenol content. Total flavonoid content was highest in S1 prepared from sugar, followed by S2 prepared from fructooligosaccharide. The DPPH (2,2-Diphenyl-1-picrylhydrazyl) radical scavenging ability was the highest in S7, prepared by mixing sugar and fructooligosaccharide; and the lowest in S3, prepared with isomaltooligosaccharide. However, S3 showed the highest value in ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging ability. S1 Omija cheong prepared from sugar showed the most inhibitory activity against α-glucosidase. S6 which was prepared using isomaltooligosaccharide that is not absorbed by internal enzymes, showed safety even at a low pH, and is considered to be the most practical method for sugar reduction.

Molecular Genetic Distances and Hybrid Performances among Maize lnbreds and Their Hybrids

Lee, Ju Kyong,Min, Hwang-Kee,Park, Jong-Yeol,Huh, Nam-Ki,Park, Ki-Jin,Choi, Jae-Keun,Park, Kyong-Cheul,Kim, Nam-Soo 한국유전학회 2002 Genes & Genomics Vol.24 No.3

Genetic variations and genetic distances of the maize inbred breeding lines were analyzed using SSR (simple sequence repeats) and AFLP (amplified fragment length polymorphisms) techniques in order to assess the agronomic performances in their hybrids. The 25 inbred lines utilized consisted of mainly two groups of Lancaster Sure Crops in BSSS and Reid Yellow Dents except one line. The genetic distances, GDs, between groups were higher than the GDs within groups. The AFLP-based GDs were higher than the SSR-based GDs in all inbreds. While the clustering patterns derived from SSR polymorphisms showed low correlations with the pedigree information, AFLP-based clustering pattern generally matched with the pedigree grouping. In the analysis of correlation of GDs with agronomic performance in the hybrids, the AFLP-GDs showed high correlations with the plant height, ear diameter, hundred seed weight, and grain yield. However, there was a low correlations between the SSR-GDs and the above traits. The possible reasons of this low correlation and ambiguity in clustering pattern using SSR analysis were sought in the discussion. The trait of ear length showed low correlation with GDs from either technique. Therefore, the AFLP analysis might provide an efficient and practical utility in predicting hybrid performance in the yield components except ear length.

Comparison of physiological activities and of useful compounds between new and waste bulbs of different lily (Lilium davidii) varieties

Tae Gyu Yi,Yeri Park,Su Jin Yang,Jung Dae Lim,Sang Un Park,Kyong Cheul Park,Nam Il Park 충남대학교 농업과학연구소 2016 Korean Journal of Agricultural Science Vol.43 No.5

Genetic differentiation of Mutator insertion polymorphisms and association with agronomic traits in waxy and common maize

Rahul Vasudeo Ramekar,Kyu Jin Sa,Kyong‑Cheul Park,Jong Yeol Park,Ki Jin Park,Ju Kyong Lee 한국유전학회 2020 Genes & Genomics Vol.42 No.6

Background As waxy maize is considered a key economic crop in Korea, an understanding of its genetic variation and differentiation is fundamental for the selective plant breeding. The maize genome is primarily composed of transposable elements, for which large and stable insertions generate variations that reflect selection during evolution. Objectives This study was to elucidate the genetic diversity based on the contribution of TEs and to investigate the effect of Mu transposition on the genetic divergence of waxy and common maize. We also performed an association analysis on these inbred lines to determine the Mu insertions associated with agronomic traits. Methods In this study, we utilized a Mutator-based transposon display method to study the genetic diversity and population structure of 40 waxy and 40 common inbred lines of maize in the Gangwon Agricultural Research and Extension Services collection at the Maize Research Institute. Results We detected polymorphisms in 86.33% of 278 Mutator (Mu) anchored loci, reflecting the activity of the Mu element and its contribution to genetic variation. Common maize showed a substantial amount of genetic diversity, which was greater than that observed in waxy maize. Principal-coordinate and neighbor-joining cluster analyzes consistently supported the presence of two genetically distinct groups. However, the distribution of genetic variation within the populations was much higher than the genetic differentiation among the populations. To explore the contribution of the Mu element to phenotypic variation, we analyzed the associations with ten important agronomical traits. On the basis of the combined results from two models (QGLM and Q + KLM), we found significant associations between seven Mu loci and four different traits. Conclusions These results will assist waxy maize breeders in choosing parental lines and be useful for marker-assisted selection.

Determination of the Authenticity of Dairy Products on the Basis of Fatty Acids and Triacylglycerols Content using GC Analysis

Park, Jung-Min,Kim, Na-Kyeong,Yang, Cheul-Young,Moon, Kyong-Whan,Kim, Jin-Man Korean Society for Food Science of Animal Resource 2014 한국축산식품학회지 Vol.34 No.3

Milk fat is an important food component, and plays a significant role in the economics, functional nutrition, and chemical properties of dairy products. Dairy products also contain nutritional resources and essential fatty acids (FAs). Because of the increasing demand for dairy products, milk fat is a common target in economic fraud. Specifically, milk fat is often replaced with cheaper or readily available vegetable oils or animal fats. In this study, a method for the discrimination of milk fat was developed, using FAs profiles, and triacylglycerols (TGs) profiles. A total of 11 samples were evaluated: four milk fats (MK), four vegetable oils (VG), two pork lards (PL), and one beef tallow (BT). Gas chromathgraphy analysis were performed, to monitor the FAs content and TGs composition in MK, VG, PL, and BT. The result showed that qualitative determination of the MK of samples adulterated with different vegetable oils and animal fats was possible by a visual comparision of FAs, using C14:0, C16:0, C18:1n9c, C18:0, and C18:2n6c, and of TGs, using C36, C38, C40, C50, C52, and C54 profiles. Overall, the objective of this study was to evaluate the potential of the use of FAs and TGs in the detection of adulterated milk fat, and accordingly characterize the samples by the adulterant oil source, and level of adulteration. Also, based on this preliminary investigation, the usefulness of this approach could be tested for other oils in the future.

Transcriptome analysis of astringent ‘Cheongdo-Bansi’ persimmon fruit treated with ethylene for removal of astringency

Park, Do Su,Tilahun, Shimeles,Park, Kyong Cheul,Choi, Ik Young,Jeong, Cheon Soon Elsevier 2019 Postharvest biology and technology Vol.150 No.-

<P><B>Abstract</B></P> <P>This research was conducted to study the gene expression related to the removal of astringency and ripening in astringent ‘Cheongdo-Bansi’ persimmon. Fruit treated with ethylene were used to compare the differentially expressed genes against fruit ripened naturally without ethylene treatment (control). We sequenced total mRNAs using Illumina high-throughput sequencing platform and constructed the transcriptome gene set by de novo assembly. We identified 93,601 unigenes with an average length of 643.2 bp in transcriptome contigs. Differential gene expression analysis was performed and a total of 12,374 unigenes were differentially expressed in the ethylene vs. control. Of these 12,374 unigenes, 6072 were up-regulated and 6302 were down-regulated in the treated fruit. Compared with the control, the number of genes that induced more than 2 fold expression were 2647 and the number of genes whose expression was inhibited more than 2 fold were 2804. We also identified 38 genes showing significantly different expression, 26 of which were up-regulated and the rest 12 genes were down-regulated. The identified genes were categorized as genes related to astringency removal, softening and other ripening-related changes. The present study will add the information on the effect of ethylene treatment for astringency removal, softening and other ripening-related changes of persimmon fruit at genomic level. This study will also contribute important resources for further study of the genes related to astringent substance for persimmon breeding and improvement.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Astringent type persimmon fruit was treated with ethylene for astringency removal. </LI> <LI> Transcriptome sequencing analysis was done and compared with the control. </LI> <LI> Genes showing significantly different expression were identified. </LI> <LI> Information was provided on astringency removal and ripening related changes. </LI> </UL> </P>

Effects of Perinatal Exposure to PhthalateI/Adipate Esters on Sex Steroid Levels and Hypothalamic Gene Expression during Early Postnatal Periods in Rats

Hwi-Cheul Lee,Gi-Sun lm.,Hak Jae Chung.,Poong-Yeon Lee,Jin-Ki Park,Won-Kyong Chang,Boh-Suk Yang,Keitaro Yamanouchi,Masugi Nishihara 한국동물생명공학회(구 한국동물번식학회) 2006 Reproductive & developmental biology Vol.30 No.4

Our previous research has identified granulin (grn) and p130 genes as sex steroidinducible genes in the rat hypothalamus, which might be involved in sexual differentiation of the brain. Phthalate esters that are used as plasticizers and also found at low levels in foods such as dairy products are often mentioned as suspected endocrine disrupters. The purpose of the present study is to elucidate whether perinatal exposure to dinbutyl phthalate (DBP), diisononyl phthalate (DINP) and di2ethylhexyl adipate (DEHA) affects hypothalamic sex steroidinducible genes. The present study assessed the effects of perinatal exposure to DBP, DINP and DEHA on sex steroid hormones levels and hypothalamic grn and p130 mRNA expressions at postnatal day (PND) 3 and 7. Pregnant rats were fed a soyfree diet containing 20, 200, 2,000 and 10,000 ppm of DBP, 40, 400, 4,000 and 20,000 ppm of DINP, or 480, 2,400 and 12,000 ppm of DEHA from gestational day (GD) 15 to GD 3 or 7. At PND 3 and 7, perinatal exposure to these chemicals did not substantially affect serum concentrations of testosterone and estradiol. At PND 3, the expression of grn mRNA levels in males was decreased by DEHA, and that of p130 was decreased by DBP, DINP and DEHA, though the effects were not dosedependent. At PND 7, the expression of grn gene in female pups was increased by higher doses of DBP and all the doses, except for 4,000 ppm, of DINP, while that in male pups decreased by 480 and 12,000 ppm of DEHA. Hypothalamic expression of p130 mRNA in males was increased by lower doses of DBP and all the doses of DINP, whereas that of females was decreased by 480 and 2,400 ppm of DEHA. These results suggest that these chemicals may affect the expression of grn and p130 genes by directly acting on the hypothalamus, thus leading to inappropriate expression of these genes.

Distribution of MITE Transposons in a Rice Genetic Map

Kyung Cheul Park,Ju Kyong Lee,Soon Jae Kwon,Ji Hyun Shin,Jeom Ho Lee,Chang In Yang,Nam Soo Kim 한국육종학회 2004 한국육종학회지 Vol.36 No.2

Molecular genetic markers have wide applicability for a various genetic analyses, and genetic mapping with PCR-based markers has identified many loci in the rice genome. This study was conducted to develop a genetic map of rice based on SSR and MITE-AFLP