野山開墾地에서 大豆栽培 硏究 : 石灰 및 窒素 供給 形態가 養分吸收 및 收量에 미치는 영향 1. Influence of Application of Lime and Kinds of Nitrogen Fertilizer on Nutrients Content and Yield

權泰午,李德培 圓光大學校 1994 論文集 Vol.28 No.2

野山 開墾地에서 대두 재배시 석회(중화량시용, 무시용) 및 질소공급 형태(무시용, 요소, 유안, 초안)에 따른 대두 生育 및 植物體 中 窒素化合物 조성과 收量등에 대하여 검토한 결과, 1.生育 및 養分吸收에 있어 석회 시용은 무시용에 비하여 식물체의 乾物重및 葉, 莖의 T-N, 水可溶性 窒素化合物, 葉의 無機成分含量등은 증가되었으나 葉의 炭水化物 함량은 감소되었으며 석회 시용구에서 窒素肥種間에는 초안구가 요소, 유안구보다도 乾物重, T-N, Amino acid-N, 무기성분함량은 증가되었으나 Allantoin-N 및 탄수화물 함량은 減少되는 경향이었다. 2.種實 中 養分含量에 있어 석회 시용구는 무시용에 비해 炭水化物, 租蛋白, CaO함량 등은 증가되었으나 K₂O함량은 감소되었고, 질소비종간에는 탄수화물, 租脂肪은 질소무시용구에서 높았으며 조단백은 유안구, CaO는 유안구 및 초안구, P₂O5는 요소구에서 대체로 높았다. 3.수량 및 수량 구성요소에 있어 석회 시용으로 무시용에 비해 百粒重 및 總莢數에서 증가되어 20%의 增收를 가져왔으며 석회 시용구에서 질소비종간에는 초안구에서 總莢數가 증가되어 질소 무시용보다 13% 증수되어 수량이 제일 높았고, 유안, 요소, 질소, 무시용순이었다. 4.葉의 T-N, Amino acid-N함량과 수량과는 正의 有意的인 相關을 나타내었으나 Allantoin-N함량과는 負의 相關을 보였고, 莖에 있어서는 生育後期 Amino acid-N함량과 수량과는 有意性이 없고, Allantoin-N함량과는 生育初期에는 負의 상관이었으나 生育後期에는 유의적인 正의 상관을 보였다. This study was carried out to investigate influence of application of lime and kinds of N fertilizer on nutrients content and yield of soybean in newly reclaimed area. Lime was applied requiring amount for neutralization and nitrogen was applied 4kg per 10a. Kinds of N fertilizer were urea, ammonium sulfate, and ammonium nitrate. Lime application caused to neutralize soil pH, and resulted in more nutrient uptake in the soybean plant than of no application of lime. As result of that, dry matter production was increased, and weight of 100 seeds and number of total pods were also increased. These factors caused to increase yield of soybean. T-sugar and starch, and crude protein etc. in seeds were increased with lime application. Ammonium nitrate under lime application resulted in more increase of dry matter production, content of inorganic elements, number of total pods, and yield than those of urea and ammonium sulfate. Dominating N form in vegetative growth stage was free amino acid-N transformed from chemical N fertilizer, but in reproductive growth stage was allantoin-N. Significant positive relationship were found between content of T-N and amino acid-N in the leaf, and yield, but negative relationship was found between content of allantoin-N and yield. Content of allantoin-N in the stem of late reproductive growth stage was significantly related to yield, but no significantly relationship was found between content of amino acid-N and yield.

대표적 근경류 생약의 방사선 방호효과

김성호,조성기,권오덕 대한방사선 방어학회 2001 방사선방어학회지 Vol.26 No.1

한의학에서 근경류(rhizomata)에 분류되어 있으며 각종 보익탕제에 주로 사용되는 대표적 생약인 백출(Baizhu, Atractylodes japonica), 천궁(Chuanxiong, Cnidium officinale), 산약(Shanyao, Discorea japonica) 및 승마(Shengma, Cimicifuga heracleifolia)의 방사선 방호효과를 확인하기 위해서 고선량 및 저선량의 방사선을 마우스에 조사하고 소장움 생존, 내재성 비장집락형성 및 apoptosis 유발 등을 관찰하였다. 방사선조사전 승마 투여군에서 소장움세포의 생존율을 증가시켰으며 내재성 비장집락 형성 시험에서는 천궁 투여군에서 평균치의 증가가 관찰되었고 천궁 및 승마는 저선량 방사선에 의한 apoptosls 형성을 억제시켰다. 이상의 결과에서 승마 및 천궁의 방사선 방호효과를 조혈세포의 생존과 회복, 소장움세포 생존을 통하여 확인하였으며 이는 독성이 적은 천연물이라는 관점에서 방사선 방호 식품으로서 적용이 가능할 것으로 사료된다. We performed this study to determine the effect of Baizhu(Atractylodes japonica), Chuanxiong(Cnidium officinale), Shanyao(Discorea japonica) and Shengma(Cimicifuga heracleifolia), as Oriental rhizomata herbs, on jejunal crypt survival, endogenous spleen colony formation and apoptosis in jejunal crypt cells of mire irradiated with high and low dose of γ-radiation. Shengma was effective in intestinal crypt survival(p<0.05). The frequency of radiation induced apoptosis was also reduced by pretreatment with Chuanxiong and Shengma(p<0.05). Although the mechanisms of this effect remain to be elucidated, these results indicated that Shengma might be a useful radioprotector, especially since it is a relatively nontoxic natural product.

한우에 감염된 Theileria sergenti merozoite의 순수분리와 genomic DNA probe에 관한 연구

채준석,이주묵,권오덕,채건상 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-

To make the genomic DNA probe of Theileria sergenti, the merozoites were purified from bovine crythrocytes. The infected erythrocytes were lysed by Aeromonas hydrophila(Ah-1) hemolysin, and the parasites were isolated by ultracentrifugation on a Percoll discontinuous density gradient. For construction of a T sergenti genomic DNA library, T sergenti DNA was digested with PstI and the fragments were ligated into the PstI site of pUC19 before transformation of Escherichia coli JM83. Out of thousands of transformants obtained by transformation of E coli JM83 with the genomic library, three plasmids were chosen. The sizes of the inserted DNAs were 2.9kb(2.4kb and 0.5kb) in pKTS1, 4.3kb in pKTS2 and 1.5kb in pKTS3, respectively. The DNA fragments used as probe KTS1(2.4kb), KTS2(4.3kb) and KTS3(1.5kb) were labeled digoxigenin-11-dUTP for the Southern hybridization. In Southern hybridization, all of the probes(KTS1, KTS2 and KTS3) reacted specifically to T sergenti DNA, but not to bovine leucocyte DNA. In order to find out the sensitivities of the digoxigenin-11-dUTP-labeled KTS1 and KTS3 as the probes, purified merozoite DNA and bovine DNA(control) were checked by dot blot hybridization with the probes. Both of the probes, KTS1 and KTS3, detected as minimum amount of 975pg of the T sergenti DNA, but not bovine DNA even to 500ng.

Effect of picolinic acid on nitric oxide synthesis in murine macrophage

Kwon, Oh-Deog,Do, Jae-Cheul,Lee, Keun-Woo The Korean Society of Veterinary Service 2002 韓國家畜衛生學會誌 Vol.25 No.4

To determine the effect of picolinic acid on NOㆍ production, murine macrophages were incubated with either medium, various concentrations of picolinic acid, or IFN-${\gamma}$ plus picolinic acid for 48 hr. Picolinic acid does not induce NOㆍ production by itself, it acted synergistically with INF-${\gamma}$ for the induction of reactive nitrogen intermediate production in murine macrophages. Thymidine incorporation appeared to be reciprocally related to nitrite levels, suggesting that IFN-${\gamma}$ plus picolinic acid induced NOㆍ synthesis exerted antiproliferative effects.

Effect of picolinic acid on nitric oxide synthesis in murine macrophage

( Oh Deog Kwon ),( Jae Cheul Do ),( Keun Woo Lee ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.4

To determine the effect of picolinic acid on NO· production, murine macrophages were incubated with either medium, various concentrations of picolinic acid, or IFN-γ plus picolinic acid for 48 hr. Picolinic acid does not induce NO· production by itself, it acted synergistically with INF-γ for the induction of reactive nitrogen intermediate production in murine macrophages. Thymidine incorporation appeared to be reciprocally related to nitrite levels, suggesting that IFN-γ plus picolinic acid induced NO· synthesis exerted antiproliferative effects.

랫드에 있어서 키토산이 납중독의 대사에 미치는 영향

권오덕(Oh-Deog Kwon),정규용(Kyu-Yong Jung) 한국독성학회 2003 Toxicological Research Vol.19 No.4

This study was carried out to investigate the effect of chitosan on lead metabolism in SD rats. Ten male rats were divided into a experimental group and a control group. Each experimental and control rats were administered by water contaminated with 100 mg/l of lead for 4 weeks, respectively. Experimental group received diets supplemented artificially with 5% of chitosan for 4 weeks. Body weight change, food and water consumption, fecal and urinary excretion, and fecal and urinary lead excretion were measured. There were no significant differences in body weight gain, food<br/> and water consumption, and fecal and urinary excretion between the two groups. However, fecal lead excretion of rats fed the diet containing 5% of chitosan were higher than the control group. Whereas urinary lead excretion of rats fed the diet containing 5% of chitosan were lower than the control group. The results suggested that the oral administration of chitosan prevents the gastrointestinal lead absorption in rats.

젖소 신생송아지의 질병발생에 관한 조사연구

권오덕 ( Oh Deog Kwon ),김남수 ( Nam Soo Kim ),채준석 ( Joon Seok Chae ),박명규 ( Myung Kyu Park ),김민석 ( Min Seog Kim ),유제춘 ( Je Chun Yoo ),이주묵 ( Joo Mook Lee ) 한국임상수의학회 2000 한국임상수의학회지 Vol.17 No.1

수송 스트레스 및 영양결핍에 기인한 일본원숭이의 집단 폐사

권오덕 ( Oh Deog Kwon ),정규식 ( Kyu Shik Jeong ),이근우 ( Keun Woo Lee ),이창민 ( Chang Min Lee ),임채웅 ( Chae Woong Lim ),도재철 ( Jae Cheul Do ) 한국가축위생학회 2003 韓國家畜衛生學會誌 Vol.26 No.2

There was a mass outbreak of death caused by transport stress and malnutrition in Japanese macaques imported from Japan at a farm stock in Korea, which were diagnosed on the ground of history taking, clinical finding, hematological examination and necropsy. Twenty eight Japanese macaques imported from Japan died suddenly from the 4th day after arrival in a farm. These animals were confined very small cages individually and lose the appetite for 10 days from shipping. Main clinical findings observed from alive Japanese macaques consisted of hypothermia and dehydration. On hematological observation for typical 4 cases showed increased erythrocytes and packed cell volume. On serum biochemical examination for 3 cases showed hypoglycemia, whereas blood urea nitrogen, aspartate aminotransferase and alanine aminotransferase were increased. On necropsy for 3 cases, hemorrhage and congestion were observed on the lung and the walls of stomach and intestine were thinned. Histologically, hemorrhagic pneumonia and enteritis with loss of villi were seen. The death of these animal was caused by poor management during transport, which was resulted in transport stress and malnutrition.

원저 : 실험적으로 유발시킨 산양의 제4위 질병에 있어서 혈액 및 제1위액의 전해질치 변화

권오덕 ( Oh Deog Kwon ),이주묵 ( Joo Mook Lee ),이현범 ( Hyun Beom Lee ),박진호 ( Jin Ho Park ),채준석 ( Joon Seok Chae ),장종식 ( Jong Sik Jang ) 한국임상수의학회 1997 한국임상수의학회지 Vol.14 No.2

개에서 피부 편평상피세포암의 발생과 치료 예

권오덕 ( Oh Deog Kwon ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.1

Squamous cell carcinoma of the skin was diagnosed in an 11-year-old, Australian shepherd dog with a hard mass on the right rump area. On histopathological examination of the tumor showed laminated keratin "pearls" surrounded by proliferated squamous cells, and mitotic figures. The dog was treated by surgical excision and chemotherapy with vinblastine sulfate, cyclophosphamide and prednisolone for 4 weeks. The tumor was effectively treated with a combination of surgery and chemotherapy.