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장지면(Ji Myun Jang),이명철(Myung Chul Lee),신준수(Joon Su Shin),정재훈(Jae Hoon Cheong),김양배(Yang Bae Kim),김박광(Bak Kwang Kim) 한국응용약물학회 2001 Biomolecules & Therapeutics(구 응용약물학회지) Vol.9 No.1
N/A The derivative of chrysin 7-O-cyclopropanecarboxylate was synthesized by condensing cyclopropanecarboxylic acid with chrysin in organic solvent, and its structure was identified by NMR, MS, UV, IR etc. We also investigated the physico-chemical properties, anti-diabetic effect and set up the quantitative analytical method of this compound. The correlation coefficient of calibration curve on this compound was approximately 0.9985 by absorption spectrophotometry. And, this study was carried out to investigate the hair-growth effect of chrysin derivative to the black mouse (C57BL/6). When this derivative in ethanol solution was administered to the back of mouse by method of skin paste, this derivative promoted the hair growth of mouse.
Eun-Ju Kim,Kwang-Myun Cheong,Ha-Kyung Joung,Bo-Hye Kim,송재영,조인수,Kyoung-Ki Lee,신연경 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.4
Infection of cattle with bovine leukemia virus (BLV) has been observed and reported worldwide, including in Korea. The onsite identificationof infected cattle would help decreasing and eradicating BLV infections on farms. Here, we present a new immunochromatographic assaythat employs monoclonal antibodies (MAbs) for the detection of antibodies against BLV in the field. BLV envelope glycoprotein (gp)51 wasexpressed in E. coli, and MAbs against recombinant BLV gp51 were generated for the development of an immunochromatographic assayto detect BLV antibodies in cattle. The sensitivity and specificity of the assay were determined by comparing these results with those obtainedfrom a standard enzyme linked immunosorbent assay (ELISA). A total of 160 bovine sera were used to evaluate the newimmunochromatographic assay. Using ELISA as a reference standard, the relative specificity and sensitivity of this assay were determinedto be 94.7% and 98%, respectively. Because of its high sensitivity and specificity, this BLV antibody detection assay would be suitable forthe onsite identification of BLV infection in the field.
Ji-Eun Yu,In-Ohk Ouh,Hyeonjeong Kang,Hye-young Lee,Kwang Myun Cheong,조인수,차상호 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.4
Porcine reproductive and respiratory syndrome (PRRS) is recognized as one of the most important infectious diseases causing serious economic loss in the swine industry worldwide. Due to its increasing genetic diversity, a rapid and accurate diagnosis is critical for PRRS control. The immunochromatographic strip test (ICST) is a rapid and convenient type of immunoassay. In this study, an on-site immunochromatographic assay-based diagnostic method was developed for detection of PRRS virus (PRRSV)-specific antibodies. The method utilized colloidal gold nanoparticle-labeled dual-type nucleocapsid proteins encoded by open reading frame 7. We evaluated 991 field samples from pig farms and 66 serum samples from experimentally PRRSV-inoculated pigs. Based on true PRRSV-specific antibody-positive or -negative sera determined by immunofluorescence assay and IgM enzyme-linked immunosorbent assay (ELISA), the specificity and sensitivity of the ICST were 97.5% and 91.1%, respectively, similar to those of a commercial ELISA (IDEXX PRRS X3 Ab). More importantly, the ICST was completed within 15 min and could detect the PRRSV-specific antibody at an earlier stage of infection (3–7 days) than that of ELISA (7+ days). The results demonstrate that the developed ICST has great potential as an on-farm diagnostic method, providing excellent diagnostic performance in a quick and convenient manner.
Development and Evaluation of a Rapid Dipstick Assay for Serodiagnosis of Bovine Brucellosis
Hannah Leah Tadeja Simborio,Alisha Wehdnesday Bernardo Reyes,Huynh Tan Hop,Lauren Togonon Arayan,Kwang Myun Cheong,김석 경상대학교 농업생명과학연구원 2016 농업생명과학연구 Vol.50 No.6
Fast, cheap and sufficient serodiagnostic tools needs to be developed for the early detectionof brucellosis. Currently the tools cannot differentiate an active infection from vaccinated, norcan it differentiate other bacterial infections with lipopolysaccharides, especially Yersiniainfections. In this study, we purified recombinant outer membrane protein 10 and 28(rOmp10,rOmp28), and a dipstick assay(indirect or sandwich) was constructed with single(rOmp10 orrOmp28) and combined rOmps(rOmp10 and rOmp28) from Brucella(B.) abortus 544 to evaluatebovine Brucella positive serum collected during the beginning of the Korean outbreak from2006 to 2015. In application with single rOmp, rOmp10(70%; indirect, 92.11%; sandwichdipstick) and rOmp28(72.5%; indirect, 86.84%; sandwich dipstick) had comparable results. Inaddition, results indicated that dipstick with combined rOmps(rOmps10 and rOmp28) weresuperior in detecting positive serum samples, at 85% indirect and 100% sandwich dipstick. Surprisingly, the results were the same in detecting negative results at 97.78% for both singleand combined indirect dipsticks. The dipstick tools with rOmp10 and rOmp28 would be usefulfor a rapid screen method for bovine brucellosis.
우인옥(In-Ohk Ouh),이주연(Ju-Yeon Lee),문서영(Seo Young Moon),송재영(Jae Young Song),장상호(Sang Ho Jang),정광면(Kwang Myun Cheong),박최규(Choi-Kyu Park),이윤희(Yoon-Hee Lee) 한국예방수의학회 2021 예방수의학회지 Vol.45 No.4
A solid-phase competition enzyme-linked immunosorbent assay (ELISA), recombinant VP2 (rVP2) protein, and monoclonal antibody (mAb) were developed for the specific and sensitive detection of porcine parvovirus (PPV) antibodies in pig sera. A total of 1,544 sera samples were collected from breeding pig farms located in the Gyeongsangbuk-do Province in the Republic of Korea. The optimal operating conditions of SC-ELISA were as follows. The concentration of rVP2 proteins coated on the wells was 4 μg/mL, the swine sera were diluted 1:2, and the HRP-conjugated PPV VP2 mAb (9A8 clone) was used at 500 ng/mL. These results suggest that the SC-rVP-ELISA assay may be a valuable alternative to the current diagnostic tools used to detect PPV-specific monoclonal antibodies and broadly monitor PPV infections in domestic pigs at different breeding stages.