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Optical coding of fusion genes using multicolor quantum dots for prostate cancer diagnosis
Lee, Hyojin,Kim, Chloe,Lee, Dongjin,Park, Jea Ho,Searson, Peter C,Lee, Kwan Hyi DOVE MEDICAL PRESS 2017 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.12 No.-
<P>Recent studies have found that prostate cancer expresses abnormal genetic markers including multiple types of <I>TMPRSS2–ERG</I> fusion genes. The expression level of different <I>TMPRSS2–ERG</I> fusion genes is correlated to pathologic variables of aggressive prostate cancer and disease progression. State-of-the-art methods for detection of <I>TMPRSS2–ERG</I> fusion genes include reverse transcription polymerase chain reaction (RT-PCR) with a detection limit of 1 fmol at urinary condition. RT-PCR is time consuming, costly, and inapplicable for multiplexing. Ability to identify multiple fusion genes in a single sample has become important for diagnostic and clinical purposes. There is a need for a sensitive diagnostic test to detect multiple <I>TMPRSS2–ERG</I> fusion genes for an early diagnosis and prognosis of prostate cancer. Here, we propose to develop an assay for prostate cancer diagnosis using oligonucleotide-functionalized quantum dot and magnetic microparticle for optical detection of rearranged <I>TMPRSS2–ERG</I> fusion genes at a low concentration in urine. We found that our assay was able to identify three different types of fusion gene with a wide detection range and detection limit of 1 fmol (almost the same level of the RT-PCR result reported). Here, we show detection of multiple <I>TMPRSS2–ERG</I> fusion genes using color-coded oligonucleotides in cell lysate and urine.</P>
이정오,이종욱,이관희,정원용,이종엽,Lee, Jeong-Oh,Lee, Jong-Wook,Lee, Kwan-Hyi,Jeung, Won-Young,Lee, Jong-Yup 한국전기화학회 2005 한국전기화학회지 Vol.8 No.3
본 연구에서는 구연산 수용액 전해질을 제조하여 전기도금 방식에 의해 III-V족 화합물 반도체 InSb를 전기화학적으로 합성하였다. 본 연구에서 제조된 InSb는 기존문헌에서 보고된 바와 달리 EPMA분석결과 In과 Sb의 조성비가 52:48로 화학양론을 정확하게 만족시키고 있고, XPS분석결과 전해질내의 구연산의 농도가 1.2M, pH가 4일 때 444.1 eV에서 InSb 화합물의 피크를 관찰하였으며 구연산의 농도가 1.2M보다 낮거나 pH가 4보다 낮을 때는 InSb화합물과 금속상태의 In이 혼재되어 있는 것을 확인하였다. 또한 XRD를 통하여 InSb(111)의 우선결정방위를 갖는다는 것을 확인하였고, I-V 특성 곡선 측정을 통해 rnSb가 고유한 반도체 특성을 보임을 확인하였다. We investigated the electrochemical formation of a stoichiometric III-V compound semiconductor of InSb from an aqueous citric solution. Under an? optimized electrochemical condition, not like other research results, the electrodeposited InSb are satisfied exactly with the stoichiometry. Furthermore it retains the inherent characteristics of III-V compound semiconductor, InSb without heat treatment. EPMA, XPS and XRD were employed for confirmation of its composition/stoichiometry, chemical state, and crystallographic orientation, respectively.
Kim, Jaeyoung,Kwon, Jung Hoon,Jang, Jinyoung,Lee, Hyojin,Kim, Seungki,Hahn, Young Ki,Kim, Sang Kyung,Lee, Kwan Hyi,Lee, Seok,Pyo, Heesoo,Song, Chang-Seon,Lee, Joonseok Elsevier 2018 Biosensors & Bioelectronics Vol.112 No.-
<P><B>Abstract</B></P> <P>Rapid and sensitive on-site detection of avian influenza virus (AIV) is the key for achieving near real-time surveillance of AIV and reducing the risk of dissemination. However, unlike the laboratory-prepared transparent buffer solutions containing a single type of influenza virus, distinction between real- and false- positive outputs and detection of low concentrations of AIV in stool specimens or cloacal swabs are difficult. Here, we developed a rapid and background-free lateral flow immunoassay (LFA) platform that utilizes near-infrared (NIR)-to-NIR upconversion nanoparticles (UCNPs) to yield a sensor that detects AIV nucleoproteins (NPs) from clinical samples within 20 min. Ca<SUP>2+</SUP> as a heterogeneous dopant ion in the shell enhanced the NIR-to-NIR upconversion photoluminescence (PL) emission without inducing significant changes in the morphology of the UCNPs. In a mixture of opaque stool samples and gold nanoparticles (GNPs), which are components of commercial AIV LFA, the background signal of the stool samples masked the absorption peak of GNPs. However, UCNPs dispersed in the stool samples still show strong emission centered at 800 nm when excited at 980 nm, which enables the NIR-to-NIR upconversion nanoparticle-based lateral flow immunoassay (NNLFA) platform to detect 10-times lower viral load than a commercial GNP-based AIV LFA. The detection limit of NNLFA for LPAI H5N2 and HPAI H5N6 viruses was 10<SUP>2</SUP> and 10<SUP>3.5</SUP> EID<SUB>50</SUB>/mL, respectively. Moreover, the viruses were successfully detected within dark brown-colored samples using the NNLFA but not the commercial AIV LFA. Therefore, the rapid and background-free NNLFA platform can be used for sensitive on-site detection of AIV.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ca<SUP>2+</SUP> as a heterogeneous dopant ion enhanced the NIR-to-NIR upconversion photoluminescence. </LI> <LI> The UCNPs dispersed in opaque stool samples still show strong NIR emission when excited at 980 nm. </LI> <LI> NNLFA showed high sensitivity for AIV detection from oropharyngeal and cloacal clinical samples. </LI> </UL> </P>
퍼멀로이(Fe_21Ni_79) 나노선재의 결정구조와 자기적 특성
李寬熙,李禹榮,李華永,鄭元容 대한금속재료학회 2002 대한금속·재료학회지 Vol.40 No.4
Fe_21Ni_79 nanowire arrays have been fabricated by the electroforming method using AAO(anodic aluminum oxide) as a template, which was prepared by anodizing the pure aluminum foil. According to the magnetic property of Fe_21Ni_79 nanowire prepared, it was found to have the coercivity more than 1 kOe due to the shape anisotropy and squareness(Mr/Ms) very close to 1. Especially, it could be noted that Fe_21Ni_79 nanowire showed the preferred crystallographic orientation of (220). Annealing treatment of Fe_21Ni_79 nanowire at 500℃ resulted in the enhancement of coercivity by 18% while the squareness was not varied by annealing treatment. However, the random orientation of Fe_21Ni_79 disk and the preferred orientation of nanowire arrays were maintained without respect to the annealing treatment up to 500℃.
Nanoscale bacteriophage biosensors beyond phage display
Lee, Jong-Wook,Song, Jangwon,Hwang, Mintai P,Lee, Kwan Hyi Dove Medical Press 2013 International journal of nanomedicine Vol.8 No.-
<P>Bacteriophages are traditionally used for the development of phage display technology. Recently, their nanosized dimensions and ease with which genetic modifications can be made to their structure and function have put them in the spotlight towards their use in a variety of biosensors. In particular, the expression of any protein or peptide on the extraluminal surface of bacteriophages is possible by genetically engineering the genome. In addition, the relatively short replication time of bacteriophages offers researchers the ability to generate mass quantities of any given bacteriophage-based biosensor. Coupled with the emergence of various biomarkers in the clinic as a means to determine pathophysiological states, the development of current and novel technologies for their detection and quantification is imperative. In this review, we categorize bacteriophages by their morphology into M13-based filamentous bacteriophages and T4- or T7-based icosahedral bacteriophages, and examine how such advantages are utilized across a variety of biosensors. In essence, we take a comprehensive approach towards recent trends in bacteriophage-based biosensor applications and discuss their outlook with regards to the field of biotechnology.</P>
교류전주법에 의해 제조된 Co(P) 나노선재의 자기적 특성과 인가전압 의존성에 관한 연구
이관희,김긍호,김이진,정원용 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.10
We have investigated the magnetic properties of Co(P) nanowire arrays fabricated by ac electroforming into self-ordered nanosized pores in anodic aluminum oxide (AAO), prepared by anodizing the pure aluminum foil. The nanopores were found to have an average diameter of 20 nm and aspect ratios of more than several hundreds. Magnetic properties of Co(P) nanowires were found to have the coercivity of more than 2.6 kOe due to the shape anisotropy and squareness(M_(r)/M_(s)) of ~0.8. It was particularly interesting to note that Co(P) nanowires showed different crystallographic structures depending on the applied potential. Co(P) nanowire prepared at ac-10 V_(rms) exhibited better magnetic properties than one prepared at ac-11 V_(rms) due to the crystallographic structural variation.
Lee, Keon ,Yong,Jang, Gun ,Hyuk,Byun, Cho ,Hyun,Jeun, Minhong,Searson, Peter ,C.,Lee, Kwan ,Hyi Portland Press Ltd. 2017 Bioscience reports Vol.37 No.3
<P>Preclinical screening with animal models is an important initial step in clinical translation of new drug delivery systems. However, establishing efficacy, biodistribution, and biotoxicity of complex, multicomponent systems in small animal models can be expensive and time-consuming. Zebrafish models represent an alternative for preclinical studies for nanoscale drug delivery systems. These models allow easy optical imaging, large sample size, and organ-specific studies, and hence an increasing number of preclinical studies are employing zebrafish models. In this review, we introduce various models and discuss recent studies of nanoscale drug delivery systems in zebrafish models. Also in the end, we proposed a guideline for the preclinical trials to accelerate the progress in this field.</P>
NH_4Cl이 함유된 전해질에서 전기화학적으로 제조된 Co(P) 합금의 미세구조와 자기적 특성
李寬熙,金肯鎬,鄭元容 대한금속재료학회 2002 대한금속·재료학회지 Vol.40 No.7
The variation of magnetic properties of electrodeposited thin film Co(P) alloys was studied using a different concentration of ammonium chloride in the solution. Using cyclic voltammetry as an electro-analytical technique, change in cathodic polarization behavior was measured. It was speculated that the kinetics of nucleation and electrocrystallization process of Co(P) alloys was modified by the concentration of ammonium chloride. The addition of ammonium chloride seems to suppress the polarization and kinetics of electrocrystallization by charge tranfer leading to a larger grain size and highly preferred orientation. Such microstructural features were confirmed by imaging and electron diffraction method in the TEM.