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Krishnan, Hari B.,Kang, Beom Ryong,Hari Krishnan, Ammulu,Kim, Kil Yong,Kim, Young Cheol American Society for Microbiology 2007 Applied and environmental microbiology Vol.73 No.1
<B>ABSTRACT</B><P>Phenazine production was engineered in <I>Rhizobium etli</I> USDA9032 by the introduction of the <I>phz</I> locus of <I>Pseudomonas chlororaphis</I> O6. Phenazine-producing <I>R. etli</I> was able to inhibit the growth of <I>Botrytis cinerea</I> and <I>Fusarium oxysporum</I> in vitro. Black bean inoculated with phenazine-producing <I>R. etli</I> produced brownish Fix<SUP>−</SUP> nodules.</P>
Krishnan Ayyasami 한국지질과학협의회 2006 Geosciences Journal Vol.10 No.3
The Cretaceous sediments of the Ariyalur area,southern India, are the largest and the most important of allthe exposures of that system in southern India. Oyster bivalvesare common in these Cretaceous sediments. More than twentyspecies belonging to fifteen genera are known from the Ariyalurresenting ages ranging from Late Albian to Maastrichtian. Theabundance, preservation and restricted ranges of these bivalvespecies in the Cretaceous of Ariyalur area ofer scope for bio-zonation. They seem to play a role as markers of sedimentaryunits by their apparent accumulation. This study attempts tointerpret the palaeoenvironment of deposition based on size,distribution and carbon isotope study of the shells, the petrol-ogy of the associated rocks and inferences based on othergroups of fossils including invertebrates, vertebrates and plantfossils.
Using Implementation Science to Advance Cancer Prevention in India
Krishnan, Suneeta,Sivaram, Sudha,Anderson, Benjamin O.,Basu, Partha,Belinson, Jerome L,Bhatla, Neerja,D' Cruz, Anil,Dhillon, Preet K.,Gupta, Prakash C.,Joshi, Niranjan,Jhulka, PK,Kailash, Uma,Kapambwe Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9
Oral, cervical and breast cancers, which are either preventable and/or amenable to early detection and treatment, are the leading causes of cancer-related morbidity and mortality in India. In this paper, we describe implementation science research priorities to catalyze the prevention and control of these cancers in India. Research priorities were organized using a framework based on the implementation science literature and the World Health Organization's definition of health systems. They addressed both community-level as well as health systems-level issues. Community-level or "pull" priorities included the need to identify effective strategies to raise public awareness and understanding of cancer prevention, monitor knowledge levels, and address fear and stigma. Health systems-level or "push" and "infrastructure" priorities included dissemination of evidence-based practices, testing of point-of-care technologies for screening and diagnosis, identification of appropriate service delivery and financing models, and assessment of strategies to enhance the health workforce. Given the extent of available evidence, it is critical that cancer prevention and treatment efforts in India are accelerated. Implementation science research can generate critical insights and evidence to inform this acceleration.
Krishnan, N. Nambi,Lee, Sangrae,Ghorpade, Ravindra V.,Konovalova, Anastasiia,Jang, Jong Hyun,Kim, Hyoung-Juhn,Han, Jonghee,Henkensmeier, Dirk,Han, Haksoo Elsevier 2018 Journal of membrane science Vol.560 No.-
<P><B>Abstract</B></P> <P>Crosslinked metal oxide containing nanocomposite membranes, in which the filler also acts as crosslinker, were prepared by blending polybenzimidazole (PBI-OO) and phenylsulfonated TiO<SUB>2</SUB> particles (s-TiO<SUB>2</SUB>). Thermal curing changes the ionically crosslinked system into a covalently crosslinked system. The synthesized s-TiO<SUB>2</SUB> nanoparticles were analyzed by thermal gravimetric analysis and scanning electron microscopy. The covalently crosslinked nanocomposite membranes (c-sTiO<SUB>2</SUB>-PBI-OO) were doped with phosphoric acid (PA) for high temperature proton exchange membrane fuel cell (HT-PEMFC) application. The membrane properties, such as PA uptake, dimensional change, gel content, proton conductivity, mechanical property, and single cell performance were evaluated and compared with the properties of acid-doped c-PBI-OO. PA doped 6-c-sTiO<SUB>2</SUB>-PBI-OO (6 wt% sTiO<SUB>2</SUB>) showed the highest uptake of 392 wt%, and a proton conductivity at 160 °C of 98 mS cm<SUP>−1</SUP>. In the fuel cell, a peak power density of 356 mW cm<SUP>−2</SUP> was obtained, which is 76% higher than that of a c-PBI-OO based system (202 mW cm<SUP>−2</SUP>). To evaluate the stability of the membrane performance over time, the best performing membrane was tested for over 700 h.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PBI-OO is filled with sulfophenylated TiO<SUB>2</SUB> nanoparticles. </LI> <LI> the ionically crosslinked structure changes into a covalent network by thermal curing. </LI> <LI> conductivity at 160 °C is 98 mS cm<SUP>−1</SUP>. </LI> <LI> peak power density in the HT-PEMFC is 356 mW cm<SUP>−2</SUP>. </LI> <LI> at 600 mA cm<SUP>−2</SUP> the voltage degrades at 55.4 µV h<SUP>−1</SUP>, indicating a stable performance. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Anaerobic Digestion and In situ Electrohydrolysis of Dairy Bio-sludge
Krishnan Vijayaraghavan,G. K. Sagar 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.3
A novel treatment method based on anaerobic digestion and in-situ electrohydrolysis of dairy bio-sludge was investigated in this article. The electrohydrolysis was carried out inside the anaerobic reactor using graphite anode and stainless steel cathode. The electrons released by the graphite anode combines with the proton released due to electrohydrolysis of fatty acids which resulted in the formation of hydrogen gas. The experiments were conducted using a DC power source under continuous and intermittent mode of input voltage ranging from 0.5 to 2.5 V for varying influent volatile solids concentration at a pH 5.3 ± 0.2. The results favored intermittent mode of input voltage rather than continuous supply. For an influent total solid concentration of 7% (64,120 mg/L VS), intermittent input voltage of 2 V, and a hydraulic retention time of 15 days resulted in a volatile solids and soluble COD removal efficiency of 83 and 74%, while the cumulative gas generation was 1,051 L with a hydrogen content of 72%.
Krishnan Giribabu,Yuvaraj Haldorai,Muruganantham Rethinasabapathy,장성찬,Ranganathan Suresh,조완섭,한영규,노창현,허윤석,Vengidusamy Narayanan 한국물리학회 2017 Current Applied Physics Vol.17 No.8
In this study, poly(methyl orange) (PMO) was synthesized by electrodeposition onto a glassy carbon electrode (GCE), and the resulting structure was examined for the determination of 4-nitrophenol (4-NP). Cyclic voltammetry revealed that the PMO-modified GCE (PMO/GCE) exhibited excellent electrocatalytic activity for the oxidation of 4-NP in a 0.5-M phosphate buffer solution. In contrast, the bare GCE showed no oxidation peak. Interestingly, PMO/GCE exhibited an oxidation peak at approximate 0.93 V, and the background current was higher than that of the bare GCE. Furthermore, the developed electrochemical sensor exhibited a linear relationship with the 4-NP concentration from 600 nM to 10 mM, and the limit of detection was 170 nM (signal/noise ¼ 3). The sensor demonstrated excellent selectivity, good stability, and reproducibility. It was applied to the determination of 4-NP in water samples by the standard addition method and gave recoveries of 99.2e100.9%.
Krishnan, Muralitharan,Yun, Sangwoon,Jung, Yoon Mo Elsevier 2018 International journal of electronics and communica Vol.97 No.-
<P><B>Abstract</B></P> <P>To maximize the network lifetime of a wireless sensor network, an efficient transmission technique is critical. The energy constraint is a crucial factor in the sensor network because the sensor nodes are typically battery-run and it is impossible or difficult to recharge them in most application scenarios. Unbalanced data transference in the communication channel frequently produces an energy hole problem, which causes the premature death of the sensor nodes and reduces the network lifetime. To resolve this issue and improve the network lifetime, the proposed approach adjusts the transmission range according to the distances between the cluster heads and their members. Furthermore, a mobile data collector based on the firefly optimization algorithm is employed to increase the network lifetime. The proposed algorithm is compared with standard benchmark algorithms in several scenarios. The simulation results demonstrate that the proposed algorithm outperforms existing algorithms with respect to the network lifetime.</P>
Krishnan, Rahul,Qadiri, Syed Shariq Nazir,Kim, Jong-Oh,Kim, Jae-Ok,Oh, Myung-Joo The Korean Society of Fisheries and Aquatic Scienc 2019 Fisheries and Aquatic Sciences Vol.22 No.12
Background: In the present study, we evaluated four commonly used housekeeping genes, viz., actin-β, elongation factor-1α (EF1α), acidic ribosomal protein (ARP), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as internal references for quantitative analysis of immune genes in nervous necrosis virus (NNV)-infected seven-band grouper, Hyporthodus septemfasciatus. Methods: Expression profiles of the four genes were estimated in 12 tissues of healthy and infected seven-band grouper. Expression stability of the genes was calculated using the delta Ct method, BestKeeper, NormFinder, and geNorm algorithms. Consensus ranking was performed using RefFinder, and statistical analysis was done using GraphpadPrism 5.0. Results: Tissue-specific variations were observed in the four tested housekeeping genes of healthy and NNV-infected seven-band grouper. Fold change calculation for interferon-1 and Mx expression using the four housekeeping genes as internal references presented varied profiles for each tissue. EF1α and actin-β was the most stable expressed gene in tissues of healthy and NNV-infected seven-band grouper, respectively. Consensus ranking using RefFinder suggested EF1α as the least variable and highly stable gene in the healthy and infected animals. Conclusions: These results suggest that EF1α can be a fairly better internal reference in comparison to other tested genes in this study during the NNV infection process. This forms the pilot study on the validation of reference genes in Hyporthodus septemfasciatus, in the context of NNV infection.
A Simple and Rapid Method to Isolate Low Molecular Weight Proteinase Inhibitors from Soybean
Krishnan Bari B. The Korean Society of Crop Science 2004 Korean journal of crop science Vol.49 No.4
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the $60\%$ isopropanol extract of soybean(Glycine max [L.] Merr.) seed revealed two abundant proteins with molecular masses of 19 and 10 kDa. Amino acid analysis revealed that the isopropanol-extractable protein fraction was rich in cysteine. Two-dimensional gel electro-phoretic analysis indicated that the 19kDa and 10kDa proteins had pI of 4.2 and 4.0 respectively. Peptide mass fingerprints of trypsin digests of the two proteins obtained using matrix-assisted, laser desorption/ionization-time of flight (MALDI-TOF) mass spectroscopy revealed the 19kDa protein was Kunitz trypsin inhibitor and the 10kDa protein was Bowman-Birk proteinase inhibitor. When resolved under non-denaturing conditions, the isopropanol-extracted proteins inhibited trypsin and chymotrypsin activity. Results presented in this study demonstrate that isopropanol extraction of soybean seed could be used as a simple and rapid method to obtain a protein fraction enriched in Kunitz trypsin and Bowman-Birk proteinase inhibitors. Since proteinase inhibitors are rich in sulfur amino acids and are putative anticarcinogens, this rapid and inexpensive isolation procedure could facilitate efforts in nutrition and cancer research.