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RISS 인기검색어
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- 저자 : Hari Krishnan, Ammulu (검색결과 19 건)
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Krishnan, Hari B.,Kang, Beom Ryong,Hari Krishnan, Ammulu,Kim, Kil Yong,Kim, Young Cheol American Society for Microbiology 2007 Applied and environmental microbiology Vol.73 No.1
<B>ABSTRACT</B><P>Phenazine production was engineered in <I>Rhizobium etli</I> USDA9032 by the introduction of the <I>phz</I> locus of <I>Pseudomonas chlororaphis</I> O6. Phenazine-producing <I>R. etli</I> was able to inhibit the growth of <I>Botrytis cinerea</I> and <I>Fusarium oxysporum</I> in vitro. Black bean inoculated with phenazine-producing <I>R. etli</I> produced brownish Fix<SUP>−</SUP> nodules.</P>
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Hari B. Krishnan 韓國作物學會 2002 한국작물학회지 Vol.47 No.2
Antibodies raised against the purified p-subunit of β -conglycinin were used in immunohistochemical studies to monitor the pattern of β -conglycinin mobilization in the cotyledons during soybean [Glycine max (L.) Merr.] seed germination. Western blot analysis revealed that the break down of the β -subunit of β -conglycinin commenced as early as 2 days after seed imbibition (DAI). Concurrent with the degradation of the β -subunit of β -conglycinin, accumulation of 48, 28, and 26 kD proteolytic intermediates was observed from 2 to 6 DAI. Western blot analysis also revealed that the acidic subunit of glycinin was mobilized earlier than the basic subunit. The basic glycinin subunit was subjected to proteolysis within 2 DAI resulting in the appearance of an intermediate product approximately 2 kD smaller than the native basic glycinin subunit. In contrast to the major seed storage proteins, lipoxygenase was subjected to limited proteolysis and was detected even after 8 DAI. The first sign of β -conglycinin breakdown was observed near the vascular strands and proceeded from the vascular strands towards the epidermis. Protein A-gold localization studies using thin sections of soybean cotyledons and antibodies raised against the β -subunit of β -conglycinin revealed intense labeling over protein bodies. A pronounced decrease in the protein A-gold labeling intensity over protein bodies was observed at later stages of seed germination. The protein bodies, which were converted into a large central vacuole by 8 DAI, contained very little 7S protein as evidenced by sparse protein A-gold labeling in the vacuoles.
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Krishnan, Hari B. The Korean Society of Crop Science 2002 한국작물학회지 Vol.47 No.2
Antibodies raised against the purified p-subunit of $\beta$-conglycinin were used in immunohistochemical studies to monitor the pattern of $\beta$-conglycinin mobilization in the cotyledons during soybean [Glycine max (L.) Merr.] seed germination. Western blot analysis revealed that the break down of the $\beta$-subunit of $\beta$-conglycinin commenced as early as 2 days after seed imbibition (DAI). Concurrent with the degradation of the $\beta$-subunit of $\beta$-conglycinin, accumulation of 48, 28, and 26 kD proteolytic intermediates was observed from 2 to 6 DAI. Western blot analysis also revealed that the acidic subunit of glycinin was mobilized earlier than the basic subunit. The basic glycinin subunit was subjected to proteolysis within 2 DAI resulting in the appearance of an intermediate product approximately 2 kD smaller than the native basic glycinin subunit. In contrast to the major seed storage proteins, lipoxygenase was subjected to limited proteolysis and was detected even after 8 DAI. The first sign of $\beta$-conglycinin breakdown was observed near the vascular strands and proceeded from the vascular strands towards the epidermis. Protein A-gold localization studies using thin sections of soybean cotyledons and antibodies raised against the $\beta$-subunit of $\beta$-conglycinin revealed intense labeling over protein bodies. A pronounced decrease in the protein A-gold labeling intensity over protein bodies was observed at later stages of seed germination. The protein bodies, which were converted into a large central vacuole by 8 DAI, contained very little 7S protein as evidenced by sparse protein A-gold labeling in the vacuoles.
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Krishnan Hari B. The Korean Society of Crop Science 2004 Korean journal of crop science Vol.49 No.4
Sinorhizobium sp. strain MUS10 forms nitrogen-fixing stem nodules on Sesbania rostrata, a tropical green manure crop. In this study, the ultrastructural events associated with the formation of stem nodules were investigated. Sinorhizobium sp. strain MUS10 entered the host tissue through cracks created by the emerging adventitious root primordia and multiplied within the intercellular spaces. During early phases of infection, host cells adjacent to invading bacteria revealed cellular damage that is typical of hypersensitive reactions, while the cells at the inner cortex exhibited meristematic activity. Infection threads were numerous in S-day-old nodules and often were associated with the host cell wall. In several cases, more than one infection thread was found in individual cells. The junction at which the host cell walls converged was often enlarged due to fusion of intracellular branches of infection threads resulting in large infection pockets. The infection threads were made up of a homogeneous, amorphous matrix that enclosed the bacteria. Several finger-like projections were seen radiating from these enlarged infection threads and were delineated from the host cytoplasm by the plasma membrane. As in Azorhizobium caulinodans induced root nodules, the release of Sinorhizobia from the infection threads into the plant cells appears to be mediated by 'infection droplets'. A 15-dayold Sesbania stem nodule revealed typical ultrastructure features of a determinate nodule, containing several bacterioids within symbiosomes.
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Hari B. Krishnan 韓國作物學會 2004 Korean journal of crop science Vol.49 No.4
Sinorhizobium sp. strain MUS10 forms nitrogen-fixing stem nodules on Sesbania rostrata, a tropical green manure crop. In this study, the ultrastructural events associated with the formation of stem nodules were investigated. Sinorhizobium sp. strain MUS10 entered the host tissue through cracks created by the emerging adventitious root primordia and multiplied within the intercellular spaces. During early phases of infection, host cells adjacent to invading bacteria revealed cellular damage that is typical of hypersensitive reactions, while the cells at the inner cortex exhibited meristematic activity. Infection threads were numerous in S-day-old nodules and often were associated with the host cell wall. In several cases, more than one infection thread was found in individual cells. The junction at which the host cell walls converged was often enlarged due to fusion of intracellular branches of infection threads resulting in large infection pockets. The infection threads were made up of a homogeneous, amorphous matrix that enclosed the bacteria. Several finger-like projections were seen radiating from these enlarged infection threads and were delineated from the host cytoplasm by the plasma membrane. As in Azorhizobium caulinodans induced root nodules, the release of Sinorhizobia from the infection threads into the plant cells appears to be mediated by 'infection droplets'. A 15-day-old Sesbania stem nodule revealed typical ultrastructure features of a determinate nodule, containing several bacterioids within symbiosomes.
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Impact of Upper Limb Joint Fluid Variation on Inflammatory Diseases Diagnosis
Hari Krishnan G,Ananda Natarajan R,Anima Nanda 대한전기학회 2014 Journal of Electrical Engineering & Technology Vol.9 No.6
Joint pain is generally a common disorder not only for the old aged people but also for the immunocompromised patients. The present proposed study reveals the presence of inflammatory diseases in joint generally diagnosed by removing synovial fluid and changes in the volume and composition are examined for the presence of WBC and crystals. This study implement a non-invasive approach to identify the changes in joint fluid by measuring the changes in electrical property of the synovial tissue under the influence of electrical current signal with frequency range between 100 kHz to 300 kHz. The response of tissue for the current signal was measured in terms of potential drop across the tissue. The hardware system design consists of input and output sections. The input section which applies current signal to upper limb joint region is made of ICL8038 function generator IC with amplifier and voltage to current converter. The output section picks voltage variation using metal surface electrode, amplifier, ADC, PIC microcontroller and LCD interface. 100 patient inclusive of normal and disease affected patients where examined for upper limb synovial fluid variation and inflammatory diseases were identified.
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A Simple and Rapid Method to Isolate Low Molecular Weight Proteinase Inhibitors from Soybean
Hari B. Krishnan 韓國作物學會 2004 Korean journal of crop science Vol.49 No.4
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the 60~% isopropanol extract of soybean(Glycine max [L.] Merr.) seed revealed two abundant proteins with molecular masses of 19 and 10 kDa. Amino acid analysis revealed that the isopropanol-extractable protein fraction was rich in cysteine. Two-dimensional gel electro-phoretic analysis indicated that the 19kDa and 10kDa proteins had pI of 4.2 and 4.0 respectively. Peptide mass fingerprints of trypsin digests of the two proteins obtained using matrix-assisted, laser desorption/ionization-time of flight (MALDI-TOF) mass spectroscopy revealed the 19kDa protein was Kunitz trypsin inhibitor and the 10kDa protein was Bowman-Birk proteinase inhibitor. When resolved under non-denaturing conditions, the isopropanol-extracted proteins inhibited trypsin and chymotrypsin activity. Results presented in this study demonstrate that isopropanol extraction of soybean seed could be used as a simple and rapid method to obtain a protein fraction enriched in Kunitz trypsin and Bowman-Birk proteinase inhibitors. Since proteinase inhibitors are rich in sulfur amino acids and are putative anticarcinogens, this rapid and inexpensive isolation procedure could facilitate efforts in nutrition and cancer research.
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Study on effect of welding speed on friction stir welded aluminium metal matrix hybrid composite
P. Gopi Krishnan,B. Suresh Babu,S. Madhu,S. J. Gowrishankar,C. Bibin,S. Saran,S. Shree Ram,A. R. Sri Hari,S.Vidyasagar 한양대학교 세라믹연구소 2021 Journal of Ceramic Processing Research Vol.22 No.5
The development of aluminum series matrix hybrid composites with ceramic reinforcements has recently caught the interestof new researchers. The addition of more than one or two reinforcements in aluminium alloy leads to the fabrication of theAluminium Metal Matrix Hybrid Composite (AMMHC). The present investigation is aimed at the development of newlydeveloped AMMHC followed by friction stir welding for obtaining the improved mechanical properties. The stir castedAA6063 with reinforcement of 5%, 10% and 15% wt. Silicon Carbide (SiC) and 10% wt. Boron Carbide (B4C) werefabricated by stir casting technique and the AMMHC with 10% each of SiC and B4C showed higher mechanical properties. Further the AMMHC are joined by friction stir welding (FSW) route. A prime experimental work has been tried out in theFSW process by varying welding speed (20, 30, 40, 50 and 60 mm/min). The effect of welding speed on AMMHCs were studiedon macro and microstructural observations followed by micro hardness and ultimate tensile. Strength, in which 40 mm/minof welding speed gives better properties than other processing condition. The weldment reveals the better joint strength withthe presence of fine particle dispersion in the microstructure.
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