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Sujung Kim,Hualin Nie,Byungki Jun,Jiseong Kim,Jeongeun Lee,Seungill Kim,Ekyune Kim,Sunhyung Kim 한국유전학회 2020 Genes & Genomics Vol.42 No.5
Background Sweet potato is easily propagated by cuttings. But the molecular biological mechanism of adventitious root formation are not yet clear. Objective To understand the molecular mechanisms of adventitious root formation from stem cuttings in sweet potato. Methods RNA-seq analysis was performed using un-rooted stem (0 day) and rooted stem (3 days). Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, comparison with Arabidopsis transcription factors (TFs) of DEGs were conducted to investigate the characteristics of genes and TFs involved in root formation. In addition, qRT-PCR analysis using roots at 0, 3, 6, 9, and 12 days after planting was performed to confirm RNA-seq reliability and related genes expression. Results 42,459 representative transcripts and 2092 DEGs were obtained through the RNA-seq analysis. The DEGs indicated the GO terms related to the single-organism metabolic process and cell periphery, and involved in the biosynthesis of secondary metabolites, and phenylpropanoid biosynthesis in KEGG pathways. The comparison with Arabidopsis thaliana TF database showed that 3 TFs (WRKY, NAC, bHLH) involved in root formation of sweet potato. qRT-PCR analysis, which was conducted to confirm the reliability of RNA-seq analysis, indicated that some metabolisms including oxidative stress and wounding, transport, hormone may be involved in adventitious root formation. Conclusions The detected genes related to secondary metabolism, some hormone (auxin, gibberellin), transports, etc. and 3 TFs (WRKY, NAC, bHLH) may have functions in adventitious roots formation. This results provide valuable resources for future research on the adventitious root formation of sweet potato.
Kim, Young-Nam,Kim, Jiseong,Lee, Jeongeun,Kim, Sujung,Lee, Keum-Ah,Kim, Sun-Hyung The Korean Society of Plant Biotechnology 2020 JOURNAL OF PLANT BIOTECHNOLOGY Vol.47 No.4
Reed (Phragmites spp.) is a rhizomatous plant of the Poaceae family and is known as high tolerant plant to heavy metal contaminants. This plant is widely recognized as a Cd root-accumulator, but improved heavy metal tolerance and uptake capacity are still required for phytoremediation efficiency. To enhance capacity of hyperaccumulator plants, ethyl methane sulfonate (EMS) as chemical mutagen has been introduced and applied to remediation approaches. This study aimed to select EMS-mutagenized reeds representing high Cd resistance and large biomass and to investigate their ability of Cd accumulation. After 6 months cultivation of M<sub>2</sub> mutant reeds under Cd stress conditions (up to 1,500 µM), we discovered seven mutant individuals that showed good performances like survivorship, vitality, and high accumulation of Cd, particularly in their roots. Compared to wild type (WT) reeds as control, on average, dry weight of mutant type (MT) reeds was larger by 2 and 1.5 times in roots and shoots, respectively. In addition, these mutant plants accumulated 6 times more Cd, mostly in the roots. In particular, MT8 reeds showed the greatest ability to accumulate Cd. These results suggest that EMS mutagenesis could generate hyperaccumulator plants with enhanced Cd tolerance and biomass, thereby contributing to improvement of phytoremediation efficiency in Cd-contaminated soil or wastewater. Further studies should focus on identifying Cd tolerance mechanisms of such EMS-mutagenized plants, developing techniques for its biomass production, and investigating the practical potential of the EMS mutants for phytoremediation.
Kim, Sujung,Kim, Eunah,Lee, Yeon Ui,Ko, Eunkyo,Park, Hyeong-Ho,Wu, Jeong Weon,Kim, Dong-Wook Springer US 2017 Nanoscale research letters Vol.12 No.1
<P>We investigated the influence of metal coating on the optical characteristics of Si nanopillar (NP) arrays with and without thin metal layers coated on the sample surface. The reflection dips of the metal-coated arrays were much broader and more pronounced than those of the bare arrays. The coated metal layers consisted of two parts—the metal disks on the Si NP top and the holey metal backreflectors on the Si substrate. The Mie-like geometrical resonance in the NPs, the localized surface plasmons in the metal disks, and the propagation of surface plasmon polariton along the backreflector/substrate interface could contribute to the reflection spectra. Finite-difference time-domain simulation results showed that the interplay of the plasmonic effects and the geometric resonance gave rise to significantly enhanced light confinement and consequent local absorption in the metal-Si hybrid nanostructures.</P>
김수정(Sujung Kim),김욱중(Wook-Joong Kim),김성대(Seong-Dae Kim) 대한전자공학회 2010 대한전자공학회 학술대회 Vol.2010 No.6
본 논문은 2차원 영상들로부터 3차원 물체 복원 시 표면 정규 벡터 힘을 이용하는 방법을 제안한다. 3차원 복원에 사용하는 색상 정합 정보는 3차원 물체 표면에만 존재하고, 불연속적이다. 또한 데이터가 표면에서 일정한 값을 가지지 않는다. 이러한 특징 때문에 3차원 물체 복원은 기존의 2차원 세분화 기법보다 더 섬세한 기법이 요구된다. 3차원 표면의 정보를 표현하고 있는 표면 정규 벡터는 경계면을 실제표면으로 끌어당기는 힘으로 이용될 수 있고, 이는 데이터의 노이즈성분에 의해 국소지점에 빠지거나, 오목한 부분을 제대로 복원하지 못하는 문제를 해결할 수 있다. 실험에서 제안한 표면 정규 벡터 힘에 기반한 커널 사용이 기존 기법들에 비해 우수한 성능을 가짐을 검증한다.