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Sensor Fusion Algorithm Design in Detecting Vehicles Using Laser Scanner and Stereo Vision
Seungki Kim,Hyunkyu Kim,Wonseok Yoo,Kunsoo Huh IEEE 2016 IEEE transactions on intelligent transportation sy Vol.17 No.4
<P>It is well known that laser scanner has better accuracy than stereo vision in detecting the distance and velocity of the obstacles, whereas stereo vision can distinguish the objects better than the laser scanner. These advantages of each sensor can be maximized by sensor fusion approach so that the obstacles in front can be detected accurately. In this paper, high-level sensor fusion for the laser scanner and stereo vision is developed for object matching between the sensors. Time synchronization, object age, and reordering algorithms are designed for robust tracking of the objects. A time-delay update algorithm is also developed to determine the process time delay of the laser scanner. The expanded laser scanner data at every 1 ms is predicted by Kalman filter and is matched with the stereo vision data at every 66 ms. A cost function is formulated to describe the object matching similarity between the sensors, and the best matching candidate is selected for theminimumcost function. The proposedmatching algorithms are verified experimentally in field tests of various maneuvering cases.</P>
Light-Induced Molecular Delivery Using Plasmonic Hybrid Nanogels
Seungki LEE,Jieun YOU,Hakchun KIM,Doyun KIM,Inhee CHOI 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Light is a kind of intuitive, easily controllable, and spatiotemporal stimuli that is advantageous in controlled molecular delivery system. Gold nanoparticles (GNPs) are well known as optically active plasmonic materials and generate the local heat by photothermal conversion effect under the resonance condition. Taking this benefit, hybrid structures with plasmonic nanoparticles have been broadly suggested for developing advanced nanocarriers. Here, we present a simple single-step integration method to obtain plasmonic hybrid nanogels (PHNs) composed of thermo-responsive polymers and GNPs. Under the resonant light illumination, PHN generates local heat enough to induce structural change of the polymeric networks and sequentially release the loaded molecules. To maximize this effect, we systematically tune the number of GNPs integrated in the PHNs by changing the co-building blocks. Then, we evaluate their capability to control the molecular delivery via intracellular behaviors of the PHNs stimulated by light. We envision that the proposed light responsive PHNs would be beneficial nanocarriers to improve and understand the controlled drug delivery system more precisely.
Kim, Jaeyoung,Kwon, Jung Hoon,Jang, Jinyoung,Lee, Hyojin,Kim, Seungki,Hahn, Young Ki,Kim, Sang Kyung,Lee, Kwan Hyi,Lee, Seok,Pyo, Heesoo,Song, Chang-Seon,Lee, Joonseok Elsevier 2018 Biosensors & Bioelectronics Vol.112 No.-
<P><B>Abstract</B></P> <P>Rapid and sensitive on-site detection of avian influenza virus (AIV) is the key for achieving near real-time surveillance of AIV and reducing the risk of dissemination. However, unlike the laboratory-prepared transparent buffer solutions containing a single type of influenza virus, distinction between real- and false- positive outputs and detection of low concentrations of AIV in stool specimens or cloacal swabs are difficult. Here, we developed a rapid and background-free lateral flow immunoassay (LFA) platform that utilizes near-infrared (NIR)-to-NIR upconversion nanoparticles (UCNPs) to yield a sensor that detects AIV nucleoproteins (NPs) from clinical samples within 20 min. Ca<SUP>2+</SUP> as a heterogeneous dopant ion in the shell enhanced the NIR-to-NIR upconversion photoluminescence (PL) emission without inducing significant changes in the morphology of the UCNPs. In a mixture of opaque stool samples and gold nanoparticles (GNPs), which are components of commercial AIV LFA, the background signal of the stool samples masked the absorption peak of GNPs. However, UCNPs dispersed in the stool samples still show strong emission centered at 800 nm when excited at 980 nm, which enables the NIR-to-NIR upconversion nanoparticle-based lateral flow immunoassay (NNLFA) platform to detect 10-times lower viral load than a commercial GNP-based AIV LFA. The detection limit of NNLFA for LPAI H5N2 and HPAI H5N6 viruses was 10<SUP>2</SUP> and 10<SUP>3.5</SUP> EID<SUB>50</SUB>/mL, respectively. Moreover, the viruses were successfully detected within dark brown-colored samples using the NNLFA but not the commercial AIV LFA. Therefore, the rapid and background-free NNLFA platform can be used for sensitive on-site detection of AIV.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ca<SUP>2+</SUP> as a heterogeneous dopant ion enhanced the NIR-to-NIR upconversion photoluminescence. </LI> <LI> The UCNPs dispersed in opaque stool samples still show strong NIR emission when excited at 980 nm. </LI> <LI> NNLFA showed high sensitivity for AIV detection from oropharyngeal and cloacal clinical samples. </LI> </UL> </P>
The formation of cyclopropane fatty acids in Salmonella enterica serovar Typhimurium
Kim, Bae Hoon,Kim, Seungki,Kim, Hyeon Guk,Lee, Jin,Lee, In Soo,Park, Yong Keun Microbiology Society 2005 Microbiology Vol.151 No.1
<P>The formation of cyclopropane fatty acid (CFA) and its role in the acid shock response in Salmonella enterica serovar Typhimurium (S. typhimurium) was investigated. Data obtained by GC/MS demonstrated that the CFA level in S. typhimurium increased upon its entry to the stationary phase, as in other bacteria. The cfa gene encoding CFA synthase was cloned, and mutants of the cfa gene were constructed by allelic exchange. A cfa mutant could not produce CFA and was sensitive to low pH. Introduction of a functional cfa gene into a cfa mutant cell made the mutant convert all unsaturated fatty acids to CFAs and partially restored resistance to low pH. Interestingly, the alternative sigma factor RpoS, which was induced during the stationary phase, affected the production of C(19) CFA but not C(17) CFA. Western blotting analysis showed that the increase in expression of CFA synthase at early stationary phase was due to the alternative sigma factor RpoS.</P>
측면충돌 시 뒷좌석 어린이 탑승자 상해 및 거동에 관한 연구
김승기(Seungki Kim),오은경(Eunkyung Oh),임종현(Jonghyun Yim),이만수(Mansu Lee) 한국자동차공학회 2015 한국자동차공학회 학술대회 및 전시회 Vol.2015 No.11
Occupants protection has been focused on frontal impact and adult dummy so far. Many people are becoming aware of rear seat occupants as large child and female recently. Large child occupants will be evaluated the KNCAP from 2017 and the ENCAP from 2016. However, there are very few crash tests with large child dummies in side impact. The objective of this study was to evaluate the kinematic and injury of large child dummies. Crash test were conducted with Q6 and Q10 years old anthropomorphic test device (ATD) in 55km/h side impact by using AEMDB (1300kg) to correlate CAE model (LS-DYNA). In This paper, safety performance of different positions of child dummy compared on the basis of the CAE case study results. That results shows how to apply restraints system and CRS (Child Restraints System) in side impact test protection.