서울大學校學生의 赤血球沈降速度에 關한 硏究 : 結核集團檢診의 疫學的方法을 爲하야

金仁達,權彛赫,車喆煥,柳運相,尹德老,林道洙 서울대학교 1957 서울대학교 論文集 Vol.5 No.-

The Eryshrocyte Sedimentation Rate on the students of Seoul National Uuiversity was discussed corelating with X-ray findings for tuberculosis mass suruey. 1) Among the students of Seoul National University, 4727 were examined by miniature radiogram and the morbidity of active tuberculosis was shown 1.7 percent 616 of students were examined by both miniature radiogram and blood sedimentation reaction and the morbidity was 2.9 per cent. 2) The screening procedure by blood sedimentation reaction in addition to miniature radiogram could prevent missing around one fourth of active cases This means that E.S.R. has a great significance on the screening procedure for tuberculosis case findings. 3) Abnormal E.S.R. was shown on 75,5 percent of active cases. Persons whose E.S.R. are beyond 3mm for 30minutes and 10mm for 60minutes are suspected tuberculosis and should be required further examination. 4) The distribution rate of E.S.R. of between 0∼6.5mm was shown on 80percent of these students who were examined and that of between 0∼10mm was shown on 88.7 percent of students. The mean was 4.8mm. Hereby it is recommended That E.S.R. must be employed as one part of the useful methods on the mass screening survey for tuberculosis. furthermore it should be recalled that most of the Korean youths show tuberculin test positive. This fact requires such procedure as blood sedimentation reaction in addition to X-ray photography urgently on the epidemiological tuberculosis survey.

생식주기 동안 흰쥐 자궁에서 발현되는 c-myc 관련 RCL 유전자의 기능

김도림,강성구 인제대학교 2004 仁濟論叢 Vol.19 No.1

자궁은 착상 임신 유지 및 분만을 위해 증식과 분비의 주기적인 시기를 겪는다. 배아가 착상하지 못한다면 자궁내막 조직은 퇴화되지만 배아 착상 시에는 자궁내막조직은 유지가 되며 임신 유지와 분만을 위한 과정이 진행된다. 이러한 자궁의 변화는 직접적으로 난소호르몬인 프로게스테론 (progesterone)과 에스트로겐(estrogen)의 영향을 받으며 간접적으로는 다양한 성장인자(growth factor), 사이토킨(cytokines)의 영향을 받는다. 발정주기에는 세포의 분화와 증식에 관련된 많은 유전자들이 발현된다. 본 연구는 생리 주기에서 발현되는 유전자를 분석하기 위하여 cDNA microarray를 통하여 실험하였다. 실험동물을 발정전기(proestrus), 발정기(estrus), 발정후기 (metestrus), 발정말기(diestrus)로 분류하였다. 자궁 적출 후 total RNA를 추출하였다. 추출한 RNA로부터 32P로 표지된 cDNA probe를 합성하였고 Atlas Rat Toxicology 1.2 Array (Clontech)에 hybridization을 하였다. 선별된 유전자로는 growth-related c-rnyc responsive protein RCL, heat shock 47-kDa protein (HSP47), cytochrome c oxidase polypeptide VIc2 (COX6C2), calreticulin (CALR)등이 있다. RCL 유전자는 RT-PCR을 이용하여 검증하였다. RCL 유전자 발현은 발정전기에 가장 높았으며 생식주기가 진행됨에 따라 감소 하였다. 본 연구를 통해 RCL유전자는 생식주기의 조절과 임신, 불임에 관한 중요한 정보를 제공할 것이며 이러한 유전자의 기작과 기능에 대한 연구가 더 필요하다. The uterus undergoes dynamic changes during the cycle and displays many features typical of developmental process. In order to be prepared for implantation, endometrium undergoes predictable, sequential phases of proliferlation and secretory changes. In the absence of an embryo the uterus will progress through a predictable series of stages ultimately terminating in tissue regression and apotosis. In the presence of an embryo, the endometrium not only is maintained, but also progress through an additional program of events. The uterus during estrus cycle synthesize a complex of signaling molecules with specific spatial and temporal modes of expression and which are critical for cell proliferlation and differentiation. The purpose of this investigation was to use cDNA microarrays to evaluate the expression of genes in rat uterus during estrus cycle. Animals were sacrificed on pro-estrus, estrus, metestrus, diestrus. Total RNA was isolated from the uterus. 32P-labelled cDNA was generated from total RNA and hybridizied with the Atlas Rat Toxicology 1.2 Array.(Clontech). Differential gene expression profiles were revealed growth-related c-myc responsive protein RCL, heat shock 47-kDa protein, cytochrome c oxidase polypeptide VIc2 and calreticulin. Reverse transcription polymerase chain reaction (RT-PCR) was used to validate the relative expression pattern of RCL gene. Using this approach we found that RCL gene expression in rat uterus was altered with estrus cycle. The identification of differentially expressed genes may provide important information in the regulation of estrus cycle, pregnancy and infertility. Our long-term goal is to determine the role of these differnetially expressed genes during estrus cycle

大羌活湯의 消炎 鎭痛作用에 관한 硏究

金度植,林起龍,李炳柱,엄기진,文永熙 조선대학교 약학연구소 1992 藥學硏究誌 Vol.14 No.1

Experimental studies were conducted to investigate for the effect of Daeganghwal-Tang(DHT) extract on the anti-inflammatory activity, analgesic activity, subacute toxicity and acute toxicity. The following results have been obtanied ; In the anti-inflammatory activity in rats, DHT extract (300,600㎎/㎏ p.o) was shown remarkable preventive effect in the edema induced by Freund's complete adjuvant and 5% acetic acid. In analgesic activity test in mouse, DHT extract(300,600㎎/㎏ p.o) was shown significant analgegic activity in mouse induced by 0.7% acetic acid. In acute toxicity test in mouse, DHT extract showed10% mortelity at DHT extract 1.200㎎/㎏ i.p. and 2,000㎎/㎏ p.o.. In subacute toxicity test in mouse. DHT extract was not showed toxicity for 19 day. Uric acid in the serum of the treated rats by Freund's complete adjuvant were slightly decreased by comparing with the control group. During the administration of DHT extract, The body weight increased, but the liver and spleen weight was not changed by comparing with the control group. .

부산지역 C형 간염 바이러스 genotype에 관한 역학적 연구

김인후,김정만,신해림,이명기,김준연,정갑열,김인식,서병성,신우원,양학도,허윤영,송주복 동아대학교 부설 산업의학연구소 1999 산업의학연구소 논총 Vol.- No.4

The authors investigated the distribution of Hepatitis C virus (HCV) genotype in blood donors with positive for anti-HCV (n=34), health check up examinees with positive for anti-HCV (n=29), and in patients with various chronic liver diseases positive for anti-HCV (n=63) in Pusan, Korea. HCV genotype was determined by using the molecular typing method through the reverse transcription - polymerase chain reaction (RT - PCR) with four type specific primers. Among 116 anti-HCV positive study subjects, 66.4% were positive HCV RNA by RT-PCR. The major HCV genotype was type Ⅱ (31.9%) and it was followed by type Ⅲ (27.6%). Two cases were type Ⅳ (1.7%). Double infection with two different HCV genotypes (mixed type) was found in three cases (2.6%). Three cases (2.6%) were not determined by the four type specific primers, it may have different subtype. Type Ⅱ was more prevalent than type Ⅲ in the blood donors and health check-up examinees, but the reverse was true in the chronic liver diseases patients including hepatocellular carcinoma patients, Type Ⅱ was more prevalent than type Ⅲ among the anti-HCV positive subjects with risk factors such as acupuncture history, surgical operation history, and transfusion history. In contrast type Ⅲ was more prevalent than type Ⅱ among the subjects without the above risk factors. It is supposed that the pathogenicity of different kind of HCV genotype might be different. The results of this study suggest that the type Ⅱ and type Ⅲ may be the major HCV genotype in Korea. The differences of HCV genotype distribution between the study groups support that the clinical significance according to the HCV genotype may be different.

<i>Trigonellae</i> Semen Enhances Sperm Motility and the Expression of the Cation Sperm Channel Proteins in Mouse Testes

Kim, Do Rim,Kim, Hyu Young,Kim, Ha Young,Chang, Mun Seog,Park, Seong Kyu Hindawi Publishing Corporation 2015 Evidence-based Complementary and Alternative Medic Vol.2015 No.-

<P>Genetic defects during spermatogenesis can lead to a reduction in sperm motility and cause male infertility. The cation channels of sperm (CatSper) play a role in the regulation of hyperactivated sperm motility in mouse testes. The effect of <I>Trigonellae</I> Semen (TS) on the male reproductive system and CatSper protein in mouse testes during spermatogenesis was examined. C57BL/c mice were divided into the following five groups: normal, cyclophosphamide- (CP-) only treated (control group), and three groups treated with varying concentrations of TS with CP (100, 500, and 1000 mg/kg TS and 100 mg/kg CP). Real-time PCR, western blot analysis, and a testosterone immunoassay were performed to assess CatSper protein levels in the five groups. Additionally, sperm cell counts and motility were examined. Results indicate that sperm motility and sperm counts increased in the TS treated groups in a dose-dependent manner (<I>p</I> < 0.01). CatSper levels were also significantly higher in the TS treated groups compared to that of the control group (<I>p</I> < 0.001). Therefore, TS treatment could enhance sperm function by promoting spermatogenesis and the expression of CatSper proteins in mouse testes.</P>

Selective Gene Express Profiles in Rat Uterus during Estrus Cycle

Kim, Do-Rim,Yu, Seong-Jin,Kim, Jee-Yun,Youm, Mi-Young,Lee, Chae-Kwan,Kang, Sung-Goo 한국수정란이식학회 2003 한국수정란이식학회 학술대회 Vol.2003 No.1

A new neutron transmutation doping system for radial irradiation uniformity

Kim Do Hyun,Lee Han Rim,김지석,Kim Myong-Seop,Park Byung-Gun 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.79 No.1

Nowadays, Neutron Transmutation Doping (NTD) of silicon ingots is successfully used to produce the n-type semiconductor. NTD’s main advantage, which is to say its uniform resistivity distribution, leads to semiconductor materials with fewer defects and, accordingly, better performance. Radial uniformity usually is achieved by rotating the ingot during irradiation. However, due to neutron attenuation, the neutron reaction rate in the ingot’s center region generally is lower than in the outer region. Such radial non-uniformity becomes severe when the diameter of the ingot is larger, or when materials with a high-neutron-reaction cross-section are used. In this study, a slit method to selectively shield neutrons that react with the ingot’s outer region and increase radial uniformity thereby was devised and herein is proposed. Dark matter (DM), graphite, stainless steel (SS) and borated stainless steel (BSS) were considered as shielding materials in a Monte Carlo-simulation-based evaluation of the radial uniformity of a Silicon Carbide (SiC) ingot. In the case of DM, the relative reaction rate at the center region of the ingot was increased; graphite did not enhance the radial uniformity; for both SS and BSS, the radial uniformity was under 1% with the optimized slot size. Therefore, it is expected that if the NTD technique is used in concert with the proposed slit method, semiconductors of exceedingly uniform dopant distribution can be produced.

Flavonoids Isolated from Citrus Aurantium Inhibit G2/M Phase in Human Gastric Cancer AGS Cell Line via Suppression of Protein Expression Mediated Cell Cycle

Do Hoon Lee,Kwang Il Park,Sang Rim Kang,Hyeon Soo Park,Jean A Kim,Arulkumar N,Eun Hee Kim,Gon Sup Kim 대한수의학회 2011 대한수의학회 학술대회발표집 Vol.2011 No.-

A multicenter study of entecavir <i>vs.</i> tenofovir on prognosis of treatment-naïve chronic hepatitis B in South Korea

Kim, Seung Up,Seo, Yeon Seok,Lee, Han Ah,Kim, Mi Na,Lee, Yu Rim,Lee, Hye Won,Park, Jun Yong,Kim, Do Young,Ahn, Sang Hoon,Han, Kwang-Hyub,Hwang, Seong Gyu,Rim, Kyu Sung,Um, Soon Ho,Tak, Won Young,Kweon Elsevier 2019 Journal of hepatology Vol.71 No.3

<P><B>Background & Aims</B></P> <P>It is currently unclear which antiviral agent, entecavir (ETV) or tenofovir disoproxil fumarate (TDF), is superior for improving prognosis in patients with chronic hepatitis B (CHB). Here, we assessed the ability of these 2 antivirals to prevent liver-disease progression in treatment-naïve patients with CHB.</P> <P><B>Methods</B></P> <P>From 2012 to 2014, treatment-naïve patients with CHB who received ETV or TDF as a first-line antiviral agent were recruited from 4 academic teaching hospitals. Patients with decompensated cirrhosis or hepatocellular carcinoma (HCC) at enrollment were excluded. Cumulative probabilities of HCC and death or orthotopic liver transplant (OLT) were assessed.</P> <P><B>Results</B></P> <P>In total, 2,897 patients (1,484 and 1,413 in the ETV and TDF groups, respectively) were recruited. The annual HCC incidence was not statistically different between the ETV and TDF groups (1.92 <I>vs</I>. 1.69 per 100 person-years [PY], respectively; adjusted hazard ratio [HR] 0.975 [<I>p</I> = 0.852] by multivariate analysis). Propensity score (PS)-matched and inverse probability of treatment weighting (ITPW) analyses yielded similar patterns of results (HR 1.021 [<I>p</I> = 0.884] and 0.998 [<I>p</I> = 0.988], respectively). The annual incidence of death or OLT was not statistically different between the ETV and TDF groups (0.52 <I>vs</I>. 0.53 per 100 PY, respectively; adjusted HR 1.202 [<I>p</I> = 0.451]). PS-matched and ITPW analyses yielded similar patterns of results (HR 1.248 [<I>p</I> = 0.385] and 1.239 [<I>p</I> = 0.360], respectively). These findings were consistently reproduced in patients with compensated cirrhosis (all <I>p</I> >0.05).</P> <P><B>Conclusions</B></P> <P>The overall prognosis in terms of HCC and death or OLT was not statistically different between the ETV and TDF groups. Further studies are needed to validate our results.</P> <P><B>Lay summary</B></P> <P>It is currently unclear which antiviral agent, entecavir or tenofovir disoproxil fumarate, is superior for improving prognosis in patients with chronic hepatitis B virus infection. In this analysis we found that there was no difference in terms of overall prognosis, including risk of hepatocellular carcinoma, death, or the need for a liver transplant, in patients receiving either antiviral.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The hepatocellular carcinoma risk was not statistically different between the ETV and TDF groups. </LI> <LI> The death or liver transplant risk was not statistically different between the 2 groups. </LI> <LI> These results were consistently reproduced after adjusting for confounding variables. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Development of advanced rigorous two-step code system for evaluation of radioactive waste with high-resolution activation calculation

Kim, Do Hyun,Kim, Jiseok,Lee, Han Rim,Sun, Gwang Min,Shin, Chang Ho,Kim, Jong Kyung Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.6

Nowadays, evaluation of amounts and distributions of radioactive waste is an important preparatory step in the process of nuclear reactor decommissioning. For tentative estimation of radioactive waste, a cell-based rigorous 2 step (R2S) method usually is used; however, a poor resolution caused by the averaged flux and spectrum in a cell is still a great challenge because of leading to underestimated or overestimated results. To overcome the poor resolution, several systems were introduced. Neither system, however, provides any function for evaluation of radioactive waste amount and distribution. Thus, it is additionally required to classify radioactive waste based on the results of activation calculation. In this study, the advanced R2S (AR2S) system was developed. To verify the performance of the system, its results for a verification problem were compared with those of the cell-based R2S method. The results showed good agreement, which is to say, within 2.0% relative error. Also, several characteristics of fine/coarse mesh were analyzed. To demonstrate the performance of the AR2S system, the radioactive waste from the Japan Power Demonstration Reactor (JPDR) was estimated, and the result indicated a high-resolution distribution. Therefore, it is expected that the AR2S system will prove useful for precise evaluation of radioactive waste.