Prognostic Value of MGMT Promoter Methylation and TP53 Mutation in Glioblastomas Depends on IDH1 Mutation

Wang, Kai,Wang, Yin-Yan,Ma, Jun,Wang, Jiang-Fei,Li, Shao-Wu,Jiang, Tao,Dai, Jian-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Several molecular markers have been proposed as predictors of outcome in patients with glioblastomas. We investigated the prognostic significance of $O^6$-methylguanine-DNA methyltransferase (MGMT) promoter methylation and TP53 mutation status dependent on isocitrate dehydrogenase 1 (IDH1) mutation in glioblastoma patients. A cohort of 78 patients with histologically confirmed glioblastomas treated with radiation therapy and chemotherapy were reviewed retrospectively. We evaluated the prognostic value of MGMT promoter methylation and TP53 mutation status with regard to progression-free survival (PFS) and overall survival (OS). It was revealed that mutations in IDH1, promoter methylation of MGMT, TP53 mutation, age, Karnofsky performance status (KFS), and extension of resection were independent prognostic factors. In patients with an IDH1 mutation, those with an MGMT methylation were associated with longer PFS (p=0.016) and OS (p=0.013). Nevertheless, the presence of TP53 mutation could stratify the PFS and OS of patients with IDH1 wild type (p=0.003 and 0.029 respectively, log-rank). The MGMT promoter methylation and TP53 mutation were associated with a favorable outcome of patients with and without mutant IDH1, respectively. The results indicate that glioblastomas with MGMT methylation or TP53 mutations have improved survival that may be influenced by IDH1 mutation status.

Survey on electrochemical properties of La_2−xSr_xNiO_4±δ (x = 0.2 and 0.8, δ > 0) cathodes related with structural stability under cathodic polarization conditions

Wang, Ya-Ping,Xu, Qing,Huang, Duan-Ping,Zhao, Kai,Chen, Min,Kim, Bok-Hee Elsevier 2017 International journal of hydrogen energy Vol.42 No.9

<P><B>Abstract</B></P> <P>In view of utilization as cathodes for intermediate temperature solid oxide fuel cells (SOFCs), the La<SUB>2−x</SUB>Sr<SUB>x</SUB>NiO<SUB>4±δ</SUB> (x = 0.2 and 0.8, δ > 0) series of compounds were evaluated with respect to their electrical conducting, thermomechanical and electrochemical properties. On the one hand, La<SUB>1.2</SUB>Sr<SUB>0.8</SUB>NiO<SUB>4−δ</SUB> exhibits a higher electrical conductivity (>180 S cm<SUP>−1</SUP> in the temperature range 600–800 °C) and a lower electrode polarization under open-circuit voltage conditions (∼0.12 Ω cm<SUP>2</SUP> at 800 °C) than La<SUB>1.8</SUB>Sr<SUB>0.2</SUB>NiO<SUB>4+δ</SUB>, together with a suitable thermal expansion coefficient (13.8 × 10<SUP>−6</SUP> K<SUP>−1</SUP> averaged between 50 and 1000 °C). On the other hand, the structure and electrochemical properties of La<SUB>1.2</SUB>Sr<SUB>0.8</SUB>NiO<SUB>4−δ</SUB> electrode were obviously changed after being subjected to cathodic polarization. The cathodically polarized La<SUB>1.2</SUB>Sr<SUB>0.8</SUB>NiO<SUB>4−δ</SUB> electrode showed an aggregation of strontium oxide onto the surface, concomitant with a degradation of electrochemical properties. By contrast, the structure and electrochemical properties of La<SUB>1.8</SUB>Sr<SUB>0.2</SUB>NiO<SUB>4+δ</SUB> electrode before and after cathodic polarization were basically unvaried. The disparate changes in structure and electrochemical properties of the two electrodes were interpreted in terms of their differing peculiarities in oxygen nonstoichiometry. The results of this work highlight the importance of strontium content and thereby oxygen nonstoichiometry of La<SUB>1−x</SUB>Sr<SUB>x</SUB>NiO<SUB>4±δ</SUB> electrodes to their structural stability under cathodic polarization conditions, which is a critical point to be used as cathodes in SOFCs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> LSN2 and LSN8 were evaluated in view of cathode utilization for IT-SOFCs. </LI> <LI> LSN8 exhibits higher electrical conductivity and catalytic activity than LSN2. </LI> <LI> Cathodically polarized LSN8 electrode undergoes structure and property changes. </LI> <LI> The structure and properties of polarized LSN2 electrode remains unchanged. </LI> <LI> The disparate behaviors of the two electrodes were interpreted. </LI> </UL> </P>

Diagnosis on improved electrocatalytic activity of La₂Ni_0.8Cu_0.2O_4+δ electrodes towards oxygen reduction reaction

Wang, Ya-Ping,Xu, Qing,Huang, Duan-Ping,Zhao, Kai,Chen, Min,Kim, Bok-Hee Elsevier 2017 APPLIED SURFACE SCIENCE - Vol.423 No.-

<P><B>Abstract</B></P> <P>La<SUB>2</SUB>Ni<SUB>0.8</SUB>Cu<SUB>0.2</SUB>O<SUB>4+δ</SUB> electrodes with different thicknesses were prepared on Ce<SUB>0.8</SUB>Sm<SUB>0.2</SUB>O<SUB>1.9</SUB> electrolyte using screen printing technique. The electrocatalytic activity of La<SUB>2</SUB>Ni<SUB>0.8</SUB>Cu<SUB>0.2</SUB>O<SUB>4+δ</SUB> electrodes towards oxygen reduction reaction was investigated in comparison with La<SUB>2</SUB>NiO<SUB>4+δ</SUB> electrode. To understand the electrocatalytic activity of the two electrodes, chemical states of constituent elements on the surface of La<SUB>2</SUB>NiO<SUB>4+δ</SUB> and La<SUB>2</SUB>Ni<SUB>0.8</SUB>Cu<SUB>0.2</SUB>O<SUB>4+δ</SUB> were analyzed using X-ray photoelectron spectroscopy technique. La<SUB>2</SUB>Ni<SUB>0.8</SUB>Cu<SUB>0.2</SUB>O<SUB>4+δ</SUB> electrodes sintered at 920°C exhibited improved electrocatalytic activity relative to literature results of the electrodes sintered at higher temperatures (e.g. 1200°C). The Cu doping in La<SUB>2</SUB>NiO<SUB>4+δ</SUB> was validated to be effective in enhancing electrocatalytic activity. The electrocatalytic activity of La<SUB>2</SUB>Ni<SUB>0.8</SUB>Cu<SUB>0.2</SUB>O<SUB>4+δ</SUB> electrodes was found to be dependent on their thickness. Optimal electrocatalytic activity was achieved for the electrode with a thickness of 10μm, showing a polarization resistance of 0.13Ω cm<SUP>2</SUP> at 800°C. The effects of Cu doping and electrode thickness were diagnosed with respect to contributions of involved electrode reaction steps. The results of this work underscore that the electrocatalytic activity of La<SUB>2</SUB>Ni<SUB>0.8</SUB>Cu<SUB>0.2</SUB>O<SUB>4+δ</SUB> electrodes is dictated by dissociative adsorption process of molecular oxygen on the surface.</P> <P><B>Highlights</B></P> <P> <UL> <LI> LNC electrodes achieved improved electrocatalytic activity than literature results. </LI> <LI> The electrocatalytic activity of LNC electrodes is higher than LN electrode. </LI> <LI> The electrocatalytic activity of LNC electrodes is dependent on thickness. </LI> <LI> The optimal electrode polarization resistance of LNC is 0.13Ω cm<SUP>2</SUP> at 800°C. </LI> <LI> The effects of Cu doping and electrode thickness have been explained. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

CYP1A1 Genetic Polymorphisms and Risk for Esophageal Cancer: a Case-control Study in Central China

Yun, Yu-Xia,Wang, Yan-Ping,Wang, Peng,Cui, Li-Hong,Wang, Kai-Juan,Zhang, Jian-Ying,Dai, Li-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

The purpose of this study was to evaluate the associations of CYP1A1 genetic polymorphisms with the risk of developing esophageal cancer (EC). A case-control study was carried out in a Chinese population in which 157 hospital based EC cases and 157 population based healthy controls with 1:1 match by age and sex were included. PCR based restriction fragment length polymorphisms (PCR-RFLP) were used to detect genotypes in case and control groups. For the CYP1A1 Ile/Val polymorphism, comparing with wild genotype Ile/Ile, both the heterozygote genotype Ile/Val and the combined variant genotype Ile/Val+Val/Val increased the risk of esophageal cancer (OR: 2.05, 95%CI: 1.19-3.54, OR: 1.86, 95%CI: 1.11-3.12). No significant association was found between the CYP1A1 MspI polymorphism and EC. According to analysis of combined genotypes, the TC/AG combined genotype which contained both variant alleles of these two polymorphisms increased the risk of developing EC (OR: 2.12, 95%CI: 1.16-3.85). Our results suggested that genetic polymorphisms of CYP1A1 may increase the susceptibility to EC.

INVESTIGATING ANTECEDENTS OF BRAND VALUE CO-CREATION BEHAVIORS IN SOCIAL MEDIA BASED BRAND COMMUNITIES

Kai-Yu Wang,Yu-Ping Wu,Wen-Hai Chih 글로벌지식마케팅경영학회 2020 Global Marketing Conference Vol.2020 No.11

Directed Evolution and Mutagenesis of Lysine Decarboxylase from Hafnia alvei AS1.1009 to Improve Its Activity toward Efficient Cadaverine Production

Chen Wang,Kai Zhang,Chen Zhongjun,Heng Cai,Wan Honggui,Ping-Kai Ouyang 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3

Lysine decarboxylase (LDC) exhibits a significant role in cadaverine (1,5-pentanediamine, diaminopentane) production from lysine. In this study, an error-prone PCR and DNA shuffling were performed to improve the activity of LDC from Hafnia alvei AS1.1009 for cadaverine production. A sensitive high-throughput screening strategy based on a pH indicator was established for directed evolution of LDC. Several improved mutants were obtained from directed evolution and LDCV147F/E583G mutant showed highest activity to catalyze lysine to cadaverine. This mutant showed 1.62-fold high LDC activity when compared to wild-type. Further analysis by site-directed mutagenesis reveled that only the mutant E583G was sufficient for higher catalytic activity. Wild type LDC and mutant LDCE583G were purified by an improved method including hydrophobic chromatography. These purified enzymes were characterized and the kinetic parameters were compared between LDCE583G and WT LDC. Vmax of LDCE583G was 1.32-fold higher than that of WT LDC. Use of LDCE583G mutant showed 1.48-fold improved productivity of cadaverine when compared to wild type. The concentration of cadaverine in E. coli JM109/pTrc99a-ldc2-41 was 63.9 g/L with conversion yield of 93.4% during 5 h. These results indicate that the mutation has positive effects on improving LDC activity and a potential candidate for cadaverine production.

Production of Transgenic Stem Mustard Plants Co-expressing Coat Protein Genes of TUMV and CMV

De-Ping Guo,Wei Zhang,Ji-Shuang Chen,Kai Zhang,Qiao-Mei Wang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Enzymatic Synthesis of Theanine with L-glutamine-Zn(II) Complexes

Hao-Qi Wang,Zhong Yao,Zhi Zhou,Yun Sun,Ping Wei,Ping-Kai Ouyang 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.6

Theanine, a unique amino acid found in tea plants, has many important physiological functions. Theanine can be enzymatically synthesized via the γ-glutamyltranspeptidation reaction. In this study, we described a new method of theanine synthesis using the L-glutamine-Zn(II)(Zn(Gln)2) complex instead of glutamine as the donor,which successfully reduced the side autotranspeptidation reaction and led to higher yield of theanine. We prepared the Zn(Gln)2 complexes and showed that they are stable in liquid bulk under 9.0 pH. After using the Zn(Gln)2 in the γ-glutamyltranspeptidation reaction, we utilized HPLC and Mass spectrometry analysis to demonstrated that Zn(Gln)2was an more effective γ-glutamyl donor than glutamine. The autotranspeptidation reaction was restrained effectively. As a result, the theanine yield and the conversion rate for glutamine were vastly improved. In a reaction mixture containing 48 mM of Zn(Gln)2, 1.6 M ethylamine, and 0.5 U/mL GGT, the final concentration of theanine obtained was 61.3 mM after incubation at 37oC for three hours. The conversion rate for glutamine was 63.8%, which showed a 16.9% increase as compared to when using glutamine alone as the donor substrate.

Leader-follower formation control of USVs using APF-based adaptive fuzzy logic nonsingular terminal sliding mode control method

Tingyi Wu,Kai Xue,Ping Wang 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.4

This paper addresses the formation motion control method of unmanned surface vehicles (USVs). Combining the leader-follower formation control strategy with the path planning strategy based on the artificial potential field method, the control law is designed for a group of under-actuated USVs using an adaptive fuzzy nonsingular terminal sliding mode control method. The control goal is to make the followers start from their initial positions and finally form a specific formation shape with the leader and reach a stable state. The adaptive reaching law is proposed to reduce the system chattering. In order to reduce the formation integration time, a fuzzy logic reaching law is proposed to reduce the convergence time. Meanwhile, an adaptive tuning algorithm is used to tune the controller parameters, and the Lyapunov stability theorem is used to prove the finite-time stability. Simulation results show that the proposed approach attains a satisfactory performance, comparative simulations are provided to demonstrate the effectiveness of the approach presented.

Low-damage NH3 plasma treatment on SiO2 tunneling oxide of chemically-synthesized gold nanoparticle nonvolatile memory

Jer-Chyi Wang,Kai-Ping Chang,Chin-Hsiang Liao,Ruey-Dar Chang,Chao-Sung Lai,Li-Chun Chang 한국물리학회 2016 Current Applied Physics Vol.16 No.5

Characteristics of chemically-synthesized (CS) gold nanoparticle (Au-NP) nonvolatile memories (NVMs) with low-damage NH3 plasma treatment on a tunneling oxide (TO) layer have been investigated. Although the dot density of CS Au-NPs is decreased, the programming efficiency of memories with optimized NH3 plasma treatment condition is enhanced due to the formation of a trapezoid-like energy band diagram of the TO layer by nitrogen incorporation. With the extraction of relative permittivity and electron affinity of the TO layer, the capacitance-voltage (C-V) and programming behaviors of CS Au-NP memories with low-damage NH3 plasma treatment on the TO layer are well-fitted by the TCAD (Technology Computer-Aided-Design) simulation. Further, the built-in electric field induced by the trapezoidlike energy band diagram of the TO layer can suppress the leakage current of the TO layer, thereby improving the data retention properties. The low-damage NH3 plasma treatment that results in no plasma damage to the TO layer has been proposed to be the probable candidate for future NVM applications.