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Shi, Junqing,Aguilar Suarez, Luis E.,Yoon, Seong-Jun,Varghese, Shinto,Serpa, Carlos,Park, Soo Young,Lü,er, Larry,Roca-Sanjuá,n, Daniel,Miliá,n-Medina, Begoñ,a,Gierschner, Johan American Chemical Society 2017 JOURNAL OF PHYSICAL CHEMISTRY C - Vol.121 No.41
<P>Solid state luminescence enhancement (SLE) of conjugated organic materials has had a great impact in materials science, but a deep understanding has been rather limited to date. Here, we investigate a prototype example of SLE materials, cyano-substituted distyrylbenzene (DCS), by varying systematically and subtly the substitution pattern (inter alia of the position of the cyano-substituent) to give largely different photoresponse in fluid and solid solution as well in the crystalline state. The combination of quantitative (ultra)fast optical spectroscopic techniques, appropriate quantum-chemical methods, and structural (X-ray) data allows us to elucidate and rationalize all details of the SLE process, including steric versus electronic factors, radiative versus nonradiative decay channels, and intra- versus intermolecular contributions, providing a first holistic picture of SLE.</P>
Gan Junqing,Liu Shan,Zhang Yu,He Liangzi,Bai Lu,Liao Ran,Zhao Juan,Guo Madi,Jiang Wei,Li Jiade,Li Qi,Mu Guannan,Wu Yangjiazi,Wang Xinling,Zhang Xingli,Zhou Dan,Lv Huimin,Wang Zhengfeng,Zhang Yanqiao,Q 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
The functional role of microRNA-375 (miR-375) in the development of prostate cancer (PCa) remains controversial. Previously, we found that plasma exosomal miR-375 is significantly elevated in castration-resistant PCa (CRPC) patients compared with castration-sensitive PCa patients. Here, we aimed to determine how miR-375 modulates CRPC progression and thereafter to evaluate the therapeutic potential of human umbilical cord mesenchymal stem cell (hucMSC)-derived exosomes loaded with miR-375 antisense oligonucleotides (e-375i). We used miRNA in situ hybridization technique to evaluate miR-375 expression in PCa tissues, gain- and loss-of-function experiments to determine miR-375 function, and bioinformatic methods, dual-luciferase reporter assay, qPCR, IHC and western blotting to determine and validate the target as well as the effects of miR-375 at the molecular level. Then, e-375i complexes were assessed for their antagonizing effects against miR-375. We found that the expression of miR-375 was elevated in PCa tissues and cancer exosomes, correlating with the Gleason score. Forced expression of miR-375 enhanced the expression of EMT markers and AR but suppressed apoptosis markers, leading to enhanced proliferation, migration, invasion, and enzalutamide resistance and decreased apoptosis of PCa cells. These effects could be reversed by miR-375 silencing. Mechanistically, miR-375 directly interfered with the expression of phosphatase nonreceptor type 4 (PTPN4), which in turn stabilized phosphorylated STAT3. Application of e-375i could inhibit miR-375, upregulate PTPN4 and downregulate p-STAT3, eventually repressing the growth of PCa. Collectively, we identified a novel miR-375 target, PTPN4, that functions upstream of STAT3, and targeting miR-375 may be an alternative therapeutic for PCa, especially for CRPC with high AR levels.
Qin Lu,Yue Junqing,Guo Mingzhou,Zhang Cong,Fang Xiaoyu,Zhang Shengding,Bai Wenxue,Liu Xiansheng,Xie Min 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.5
Purpose: Although estrogen receptors (ERs) signal pathways are involved in the pathogenesis and development of asthma, their expressions and effects remain controversial. This study aimed to investigate the expressions of ERα and ERβ as well as their mechanisms in airway remodeling and mucus production in asthma. Methods: The expressions of ERα and ERβ in the airway epithelial cells of bronchial biopsies and induced sputum cells were examined by immunohistochemistry. The associations of ERs expressions with airway inflammation and remodeling were evaluated in asthmatic patients. In vitro, the regulations of ERs expressions in human bronchial epithelial cell lines were examined using western blot analysis. The epidermal growth factor (EGF)-mediated ligand-independent activation of ERα and its effect on epithelial-mesenchymal transitions (EMTs) were investigated in asthmatic epithelial cells by western blot, immunofluorescent staining, and quantitative real-time polymerase chain reaction. Results: ERα and ERβ were expressed on both bronchial epithelial cells and induced sputum cells, and the expressions showed no sex difference. Compared to controls, male asthmatic patients had higher levels of ERα on the bronchial epithelium, and there were cell-specific expressions of ERα and ERβ in induced sputum. The expression of ERα in the airway epithelium was inversely correlated to forced expiratory volume in 1 second (FEV1) % and FEV1/forced vital capacity. Severe asthmatic patients had significantly greater levels of ERα in the airway epithelium than mild-moderate patients. ERα level was positively correlated with the thickness of the subepithelial basement membrane and airway epithelium. In vitro, co-stimulation of interleukin (IL)-4 and EGF increased the expression of ERα and promoted its nuclear translocation. EGF activated the phosphorylation of ERα via extracellular signal-regulated kinase and c-Jun N-terminal kinase pathways. ERα knockdown alleviated EGF-mediated EMTs and mucus production in airway epithelial cells of asthma. Conclusions: ERα contributes to asthmatic airway remodeling and mucus production through the EGF-mediated ligand-independent pathway.
Nishat Arshi,Junqing Lu,이찬규,구본흔,Faheem Ahmed 대한금속·재료학회 2013 ELECTRONIC MATERIALS LETTERS Vol.9 No.6
In this work, the effect of deposition power on the structural, morphological, and electrical properties of e-beam evaporated tantalum films was investigated. The as-deposited tantalum films were characterized using x-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy dispersive x-ray (EDX) and four-point probe resistivity measurements. The XRD patterns revealed the growth of a tetragonal crystalline structure (β-Ta), and the crystallinity of the films increased with increased deposition power. The FESEM images showed that the tantalum film morphology was smooth with fine spherical particles on the surface, and the grain size was found to increase with deposition power. The resistivity of the tantalum films decreased with increased deposition power.
Arshi, Nishat,Ahmed, Faheem,Kumar, Shalendra,Anwar, M.S.,Lu, Junqing,Koo, Bon Heun,Lee, Chan Gyu Elsevier 2011 Current Applied Physics Vol.11 No.1
<P><B>Abstract</B></P><P>We report a simple one step microwave irradiation method for the synthesis of gold nanoparticles using citric acid as reducing agent and cetyl trimethyl ammonium bromide (CTAB) as binding agent. The reaction was completed under two different microwave irradiation times (40 s and 70 s) for the production of two types of gold nanoparticles. The synthesized nanoparticles were characterized using UV–Vis absorption spectroscopy and transmission electron microscopy (TEM) measurements. UV–Vis study revealed the formation of gold nanoparticles with surface plasmon absorption maxima at 590 and 560 nm for 40 and 70 s irradiation time respectively. From TEM analysis, it is observed that the gold nanoparticles have spherical shape with particle size distribution in the range 1–10 nm and 1–2 nm for 40 s and 70 s irradiation time respectively. Antibacterial activity of gold nanoparticles as a function of particle concentration against gram-negative bacterium <I>Escherichia coli</I> (<I>E. coli</I>) was carried out in solid growth media. The two types of gold nanoparticles show high antibacterial activity with zone of inhibition of about 22 mm against <I>E. coli</I> (ATCC 25922 strain). Very small difference in the antibacterial activity for the two types of gold nanoparticles were observed. Though nanoparticles synthesized for 70 s irradiation time show slightly better antibacterial activity.</P> <P><B>Highlights</B></P><P>► Gold nanoparticles have been successfully synthesized using microwave irradiation technique. ► The mean diameter was ∼ 4.05 nm for 40 s and ∼1.05 nm for 70 s. ► Characteristic absorption peak was found to be 590 nm for 40 s and 560 nm for 70 s. ► The zone of inhibition for the two types of nanoparticles was almost similar (22 mm). ► Smaller size nanoparticles synthesized for 70s showed a slightly better antibacterial action.</P>