고환수염전의 초음파소견

허정식,최국명 제주대학교 생명과학연구소 2002 제주생명과학연구 Vol.5 No.6

Purpose: Testicular pain or swelling, often referred to as the acute scrotum, can have a number of causes. Common cause of acute scrotal pain include testicular torsion, epidiymitis, testicular appendiceal torsion, and trauma. Color Doppler ultrasonography is being used increasingly in the evaluation of acute scrotum. Diagnostic accuracy is required to avoid the loss of testicular function in the case of testicular torsion and avoid unnecessary surgery in the other causes, we find the ultrasonographic finding of testicular appendiceal torsion. Materials and Methods: We retrospectively reviewed the hospital charts of 26 cases with acute scrotum who had been admitted to our hospital between January 1997 and November 2001 with the except of scrotal trauma. In the event of acute scrotum being suspected, an imaging study was performed. The 11 cases were evaluated with respect to accuracy of diagnosis and outcome of treatment. Results: The incidence of testicular appendiceal torsion was 46% of total acute scrotum boys. Torsion of either appendage produces pain similar to that experienced with testicular torsion. Color Dopper ultrasonography demonstrates increased bood flow of epididymis and secondary hydrocele. Conclusion: The cause of an acute scrotum can usually be established based on a careful history, a thorough physical examination and appropriate diagnostic test. When the history and clinical findings are equivocal. Color Doppler Ultrasonographic finding is usually able to differentiate testicular torsion, which is surgical emergency, from testicular appendiceal torsion which can be treated with conservative management.

회음부에 위치한 다고환증 1례

허정식,최국명,김광식 제주대학교 생명과학기술혁신센터 2002 제주생명과학연구 Vol.5 No.-

Polyorchidism, defined as the presence of more than two testes, is a rare anomaly with approximately 98 cases reported in literature. We report the case of a 73-year-old man who had trichorchidism, two normal testes and one perineal testis, but he had not subjective symptom. All testes were normal in size and in shape. We have reviewed the literature.

인슐린의 세포사멸억제 작용에서 신호전달경로의 특성

허규희,허정식,김선욱,이영기,박덕배 제주대학교 생명과학기술혁신센터 2002 제주생명과학연구 Vol.5 No.-

Insulin has a variety of activities like as glucose uptake, inhibition of lipolysis, glycogen biosynthesis in a number of cells and tissues. Recently insulin has known to exert an antiapoptotic activity in different cell systems. However, it is not clear by which insulin can protect apoptosis cells from apoptosis. Thus, CHO cells expressing the human insulin receptors (CHO-IR) were used to evaluate the importances of different signaling steps activated by insulin in this process. The Ras/Raf-1/MEK/ERK cascade and the serine/threonine protein kinase Akt are two survival pathways that may be activated in response to insulin. We tested the hypothesis that inhibition of farnesylated Ras was casually related to manumycin-induced apoptosis and showed that the response to manumycin was found to be independent of K-Ras function. Moreover, blocking Ras/Raf-1/MEK/ERK cascade by the expression of a transdominant inhibitory mSOSI mutant in CHO-IR cells kept cells to sensitive to the antiapoptotic action of insulin. Insulin-dependent activation of Akt was blocked by 4th treatment with manumycin. This study suggest that the deplection of short-lived farnesylated proteins by manumycin suppresses the antiapoptotic action of insulin at least in part by disrupting Akt­activation but not of the K-Ras/Raf-1/MEK/ERK-dependent cascade.

요로감염에서 대장균의 항생제 저항성 변화에 대한 고찰

허정식,윤여민,홍정연 제주대학교 생명과학연구소 2003 제주생명과학연구 Vol.6 No.1

Purpose: Escherichia coli is the most frequent pathogenic agent of urinary tract infection, causing a wide spectrum of clinical syndromes from asymptomatic cystitis to pyelonephritis or sepsis. We studied the changes of antibiotic sensitivity to E.coli of urinary tract infection for alternative years in order to give some useful informations about the choice of adequate drug in the treatment of UTI. causing E.coli. Materials and Methods: We retrospectively reviewed the hospital charts of 185 cases with causative E.coli of urinary tract infection, who were admitted to or visited the hospital and had more than 10^(5)cfu/ml on urine culture in 1998, 2000, 2001 and 2002. Results: In E.coli isolates, resistance to six antibiotics changed significantly: ampicillin(57.14%, 84.38%, 85.07%, 55.10%), amoxacillin+clavulate(44.44%, 16,42%, 4.17%), ticacillin(84.62%, 75%, 86.67%, 51.14%), ticacillin-clavulate (0%, 51.35%, 11.94%, 4.08%), and trimethoprim(0%, 75%, 59.62%, 28.57%). Conclusion: Using ampicillin, trimethoprim/sulfamethoxazole as the first choice in the treatment of UTI should be reconsidered in Jeju.

등줄쥐의 대뇌겉질에 분포하는 NADPH-diaphorase와 Neuropeptide Y 양성반응신경세포에 관한 연구

허영범,황승준,조정식 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.1

흰쥐 시상하부의 신경세포에서 Nicotinamide Adenine DinucleotidePhosphate-Diaphorase와 Tyrosine Hydroxylase의 공존

허영범,송창섭,안희경,허영범,조정식,송창섭,안희경 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.1

GlycinecinA의 분비에 관여하는 secretor gene의 cloning

김영미,허정식,허규희 제주대학교 생명과학기술혁신센터 2002 제주생명과학연구 Vol.5 No.-

Xanthomonas campestris pv. glycines 8ra produces bacteriocin, glycinecin, which inhibits the growth of bacteria specifically belong to Xanthomonas spp. Xanthomonas spp. includes many important plant pathogenic bacteria such as X. c. pv. vesicatoria which cause the bacterial leaf spot in pepper and tomato and X. oryzae pv. oryzae which causes bacterial leaf blight in rice. Glycinecin has many of good properites as a biological control agent. Since glycinecin inhibit the growth of phytopathogenic Xanthomonas sp, it can be used to control disease without any harm to beneficial bacteria for pant mostly belonged to Pseudomonas sp. However, it needs some modification to develop glycinecin as a effective bilogical control agent. The clone involved in the secretion of bacteriocin production were isolated and named glyAsec.

흰민들레 (Taraxacum coreanum) 추출물이 급성 수은 중독된 생쥐의 간에 미치는 효과

정민주,윤중식,허진,노영복,최영복,김종세,이현화 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.1

본 연구는 급성 수은 독성에 대한 민들레의 효과를 알아보기 위하여 시도되었다. 30 g 내외의 생쥐를 대상으로 대조군, 수은(5mg/kg) 투여군, 수은 투여 후 민들레(3 g/kg)를 구강투여 한 후 다시 24, 48, 72, 96시간, 1주일군으로 세분하여 간장 손상 억제 효과를 알아보기 위하여 생화학적 및 조직학적 실험을 실시하였다. 혈액중 aspartate amiotransferase (AST)와 alanine aminotransferase (ALT)의 수치는 민들레 투여군이 수은 투여군보다 감소되었다. 또한 간조직의 SOD와 catalase 활성도 역시 민들레 투여군이 수은 투여군에 비하여 감소하였으나 통계학적 유의성은 없었다. 간 조직의 광학현미경적 관찰에서 심한 조직괴사가 관찰되지만 민들레 투여군에서는 문맥주위의 약간의 괴사와 심한 호중구 침윤현상이 관찰되었다. 전자현미경적 관찰 결과, 간장에서 수은 투여군은 간세포의 핵이 함입되어 불규칙했으며 미토콘드리아와 조면소포체의 수조가 팽대되고, 리보솜의 탈락이 관찰되었다. 민들레 투여군은 핵이 정상적인 상태로 관찰되었고, 전자밀도가 높은 미토콘드리아가 분포되어 있었으며, 리보솜이 부착된 상태로 층판구조를 형성하는 조면소포체가 관찰되었다. 이상의 연구 결과로 보아 민들레가 수은으로 유발된 간손상을 보호하는 효과가 있는 것으로 사료되며 민들레에 대한 다양한 연구가 필요할 것으로 사료된다. Dandelion has been frequently used as a remedy for women’s disease, inflammatory diseases and disorders of the liver and gallbladder. Dandelion extracts water extract, an herbal medication, may have an effect on the activity of hepatic antioxidant enzymes in diabetic rat. This study aims demonstrate the effect of dandelion extracts, one of the natural chelator, on the biochemical and enzyme activity changes in the mouse liver caused by HgCl₂. Mice approximately 30 gm in weight were grouped into the control, mercury chloride-treated, and the dandelion extractstreated after mercury chloride groups. HgCl₂ (5 mg/kg) and dandelion extracts (3 g/kg) were delivered orally. Serum AST and ALT were measured, enzyme activity of liver were examined by spectrophotometer and ultrastructural alteration of liver were examined by light and electron microscopy. Dandelion extracts were decreased the increase of serum AST and ALT level induced by mercury. The catalase activity was decreased in the dandelion extracts group. The activity of SOD was dereased, but did not show significant differences. Mercury chloride-treated hepatic cell were irregular nucleus, enlarged and reduced number of mitochodria, enlarged rough endoplasmic reticulum, loss of ribosomes. Cells treated with dandelion extracts were similar to those of the control group. In conclusion, dandelion extracts may protect the mercury-induced toxicity on Liver.

무증상적 현미경적 혈뇨의 진단

김현주,김광식,허정식 제주대학교 생명과학연구소 2002 제주생명과학연구 Vol.5 No.6

Hematuria is one of the most common problems to medical service. Blood in the urine (hematuria) may originate from any site along the urinary tract. Serious urologic lesions have been reported in 4.8% to 16.5% of patients referred for asymptomatic microscopic hematuria. The purpose of evaluating any patient with asymptomatic microscopic hematuria is significant urologic disease at an early stage when it is amenable to curative treatment and prior to it causing significant morbidity. The commonly accepted diagnostic algorithm for hematuria includes intravenous urograpy and cystoscopy. Full Urologic evaluation (IVP< or renal sonography>, urine cytology and cystoscopy) is warranted in the majority of patients referred to a urologist with asymptomatic microscopic hematuria. The most commonly recommended surveillance protocol includes urinalysis and urine cytology every 6 months with cystoscopy and intravenous urography either yearly or alternating biannually.

생후 등줄쥐의 대뇌겉질 및 줄무늬체에서 NADPH-diaphorase 양성신경세포에 관한 연구

황승준,이종환,홍혜남,이흥식,허영범,조정식 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.1