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Assessment on Antioxidant Properties of Oplopanax elatus Nakai in vitro
Jun-Heong Kim,Seok-Hyun Eom,Han-Shin Lee,Jae-Kwang Kim,Chang-Yeon Yu,Yong-Soo Kwon,Ju-Kyong Lee,Myong-Jo Kim 韓國藥用作物學會 2007 한국약용작물학회지 Vol.15 No.2
Effects of Oplopanax elatus have known to various pharmaceutical therapies. However, chemical prosperities in the plant are rarely investigated. In order to detect biological activity, we evaluated the antioxidant activity of five fractions from methanolic extracts in each part of O. elatus. Also, contents of polyphenols and polysaccharides were measured. Five fractions were of sub-fractions using n-hexane, ethyl-acetate, n-butanol, and water from methanolic extracts. Ethylacetate and n-butanol fractions from stem and root exhibited strong antioxidant activity and high total phenolics content. On the HPLC analysis, ten free phenolics, including p-hydroxybenzoic acid, chlorogenic acid, caffeic acid, syringic acid, p-cou maric acid, ferulic acid, naringin, hesperidin, quercetin and trans-cinnamic acid, were identified from the fractions and were shown to different quantitative proportions. Furthermore, ethylacetate and n-butanol fraction had the highest amount of chlorogenic acids, one of the cinnamic acid derivatives possessing pharmacological properties. These results indicated that the fractions of O. elatus, as well as methanolic extracts, could be used as natural antioxidative ingredients.
Quantitation of Formate in Plants and Its Enhancement in Response to Environmental Stresses
Kim, Jae-Kwang,Cho, Myoung-Rae,Baek, Hyung-Jin,Ryu, Tae-Hun,Kim, Jung-Bong,Kim, Jun-Heong,Kim, Myong-Jo,Yu, Chang-Yeon,Fukusaki, Ei-Ichiro,Kobayashi, Akio The Korean Society for Applied Biological Chemistr 2007 Journal of Applied Biological Chemistry (J. Appl. Vol.50 No.4
A solid-phase microextraction and gas chromatography-mass spectrometry utilizing $^{13}C$-formate as an internal standard for the determination of formate was proved to be applicable as a reliable quantitative method in several plants. We were the first to discover that trees contain larger pool sizes of formate than herbs. Our data also showed that the formate level of the leaves increased after the methanol-spraying, suggesting that methanol oxidation could convert formaldehyde into formate. In addition, drought and chilling led to the increase of endogenous formate in Arabidospsis thaliana. These results confirmed that formate is a universal stress signal in plants.
Assessment on Antioxidant Properties of Oplopanax elatus Nakai in vitro
Kim, Jun-Heong,Eom, Seok-Hyun,Lee, Han-Shin,Kim, Jae-Kwang,Yu, Chang-Yeon,Kwon, Yong-Soo,Lee, Ju-Kyong,Kim, Myong-Jo The Korean Society of Medicinal Crop Science 2007 韓國藥用作物學會誌 Vol.15 No.2
Effects of Oplopanax elatus have known to various pharmaceutical therapies. However, chemical prosperities in the plant are rarely investigated. In order to detect biological activity, we evaluated the antioxidant activity of five fractions from methanolic extracts in each part of O. elatus. Also, contents of polyphenols and polysaccharides were measured. Five fractions were of sub-fractions using n-hexane, ethyl-acetate, n-butanol, and water from methanolic extracts. Ethylacetate and n-butanol fractions from stem and root exhibited strong antioxidant activity and high total phenolics content. On the HPLC analysis, ten free phenolics, including p-hydroxybenzoic acid, chlorogenic acid, caffeic acid, syringic acid, p-cou maric acid, ferulic acid, naringin, hesperidin, quercetin and trans-cinnamic acid, were identified from the fractions and were shown to different quantitative proportions. Furthermore, ethylacetate and n-butanol fraction had the highest amount of chlorogenic acids, one of the cinnamic acid derivatives possessing pharmacological properties. These results indicated that the fractions of O. elatus, as well as methanolic extracts, could be used as natural antioxidative ingredients.
분산시스템에서 CORBA를 기반으로 한 이벤트 서비스 기법
김형준(Kim Heong Jun),이경복(Lee Kyung Bok),이재완(Lee Jae Wan) 한국정보과학회 1999 한국정보과학회 학술발표논문집 Vol.26 No.1A
정보의 효율적인 활용을 위해서는 이종의 정보자원을 효과적으로 통합해야 하며, 이러한 문제점을 해결하기 위해 서로 다른 시스템환경에서 상호 동작할 수 있는 미들웨어(Middleware)가 필요하다. 본 논문에서는 CORBA를 기반으로 하여 분산 객체들간에 이벤트 채널을 사용하여 소비자와 공급자들 사이에 결합도를 완화시켜 메시지 송수신을 위한 대기 시간을 줄였으며, 소비자와 공급자를 대신하여 메시지를 전송하는 인터페이스 객체들을 서비스 요청객체와 서비스 수신객체로 분리하여 공급자와 소비자가 동시에 양방향 메시지 전송을 할 수 있게 하였으며, 하나의 공급자가 N개의 소비자와 통신할 수 있도록 이벤트 채널을 설계하였다.
Cha Minseok,Kim Jun-Ha,Choi Hyo-Jin,Nho Soo Bin,Kim Soo-Yeon,Cha Young-Lok,Song Hyoungwoon,Lee Won-Heong,Kim Sun-Ki,Kim Soo-Jung 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.10
This work aimed to evaluate the feasibility of biohydrogen production from Barley Straw and Miscanthus. The primary obstacle in plant biomass decomposition is the recalcitrance of the biomass itself. Plant cell walls consist of cellulose, hemicellulose, and lignin, which make the plant robust to decomposition. However, the hyperthermophilic bacterium, Caldicellulosiruptor bescii, can efficiently utilize lignocellulosic feedstocks (Barley Straw and Miscanthus) for energy production, and C. bescii can now be metabolically engineered or isolated to produce more hydrogen and other biochemicals. In the present study, two strains, C. bescii JWCB001 (wild-type) and JWCB018 (ΔpyrFA Δldh ΔcbeI), were tested for their ability to increase hydrogen production from Barley Straw and Miscanthus. The JWCB018 resulted in a redirection of carbon and electron (carried by NADH) flow from lactate production to acetate and hydrogen production. JWCB018 produced ~54% and 63% more acetate and hydrogen from Barley Straw, respectively than its wild-type counterpart, JWCB001. Also, 25% more hydrogen from Miscanthus was obtained by the JWCB018 strain with 33% more acetate relative to JWCB001. It was supported that the engineered C. bescii, such as the JWCB018, can be a parental strain to get more hydrogen and other biochemicals from various biomass.
홍삼(紅蔘) 수추출물(水抽出物)이 치매단백질(痴매蛋白質) APP 형질전환(形質轉換) 초파리에 미치는 영향(影響)
김영준 ( Young Jun Kim ),김진형 ( Jin Heong Kim ),윤종현 ( Jong Hyun Yun ),정은영 ( Ejun Young Jung ),김태헌 ( Tae Heon Kim ),유영수 ( Yeoung Su Lyu ),강형원 ( Hyung Won Kang ) 대한한방신경정신과학회 2009 동의신경정신과학회지 Vol.20 No.1
Objectives: Ginseng Radix Rubra water extract(RGE) has been used in vivo test for its beneficial effects on neuronal survival and neuroprotective functions, particularly in connection with APP-related dementias and Alzheimer`s disease (AD). APP derived from proteolytic processing of the β-amyloid precursor protein (APP), including the amyloid-β peptide (Aβ), plays a critical role in the pathogenesis of Alzheimer`s dementia. Methods: We determined that RGE inhibits formation of APP, which are the behavior, and possibly causative, feature of AD. Results and Conclusions: In the cells, RGE significantly activated antiapoptosis and decreased the activity of APP-grim, a key enzyme in the apoptosis cell-signaling cascade. These results suggest that neuronal damage in AD might be due to two factors: a direct APP toxicity and multiple cellular and molecular neuroprotective mechanisms, including attenuation of apoptosis and direct inhibition of APP, underlie the neuroprotective effects of RGE.
Quantitation of Formate in Plants and Its Enhancement in Response to Environmental Stresses
Jae Kwang Kim,Myoung Rae Cho,Hyung Jin Baek,Tae Hun Ryu,Jung Bong Kim,Jun Heong Kim,Myong Jo Kim,Chang Yeon Yu,Ei Ichiro Fukusaki,Akio Kobayashi 한국응용생명화학회 2007 Journal of Applied Biological Chemistry (J. Appl. Vol.50 No.4
A Comparison on the Empirical Power of Some Normality Tests
Kim, Dae-Hak,Eom, Jun-Hyeok,Jeong, Heong-Chul 한국데이터정보과학회 2006 한국데이터정보과학회지 Vol.17 No.1
In many cases, we frequently get a desired information based on the appropriate statistical analysis of collected data sets. Lots of statistical theory rely on the assumption of the normality of the data. In this paper, we compare the empirical power of some normality tests including sample entropy quantity. Monte carlo simulation is conducted for the calculation of empirical power of considered normality tests by varying sample sizes for various distributions.