Development of the analytical method for Hexythiazox in of herbal medicine Angelica dahurica Bentham et Hooker using HPLC

Boeun Ju,Junghak Lee,Eunyoung Park,Xiu Yuan,Mai Rehan,Rakdo Go,Heeju Han,Jeong-Han Kim 한국농약과학회 2019 한국농약과학회 학술발표대회 논문집 Vol.2019 No.10

Angioleiomyoma in the Orbital Apex: A Case Report

( Boeun Lee ),( Soo Jeong Park ),( Ju Hyung Moon ),( Se Hoon Kim ),( Jong Hee Chang ),( Sun Ho Kim ),( Eui Hyun Kim ) 대한뇌종양학회·대한신경종양학회·대한소아뇌종양학회 2019 Brain Tumor Research and Treatment Vol.7 No.2

A 56-year woman presented eyeball pain and blurred vision. MRI revealed a small well-delineated solid tumor in the apex of right orbit with optic nerve compression. Intraoperatively, the tumor was found very fibrous, hypervascular and adhesive to surrounding structures. The tumor was completely removed with the combination of endoscopic and microscopic technique. Patient experienced transient oculomotor nerve palsy, which completely recovered 3 months after surgery. Herein we report a rare case of angioleiomyoma in the orbital apex.

Non-peptidic guanidinium-functionalized silica nanoparticles as selective mitochondria-targeting drug nanocarriers

Ahn, Junho,Lee, Boeun,Choi, Yeonweon,Jin, Hanyong,Lim, Na Young,Park, Jaehyeon,Kim, Ju Hyun,Bae, Jeehyeon,Jung, Jong Hwa The Royal Society of Chemistry 2018 Journal of materials chemistry. B, Materials for b Vol.6 No.36

<P>We report on the design and fabrication of a Fe3O4 core-mesoporous silica nanoparticle shell (Fe3O4@MSNs)-based mitochondria-targeting drug nanocarrier. A guanidinium derivative (GA) was conjugated onto the Fe3O4@MSNs as the mitochondria-targeting ligand. The fabrication of the Fe3O4@MSNs and their functionalization with GA were carried out by the sol-gel polymerization of alkoxysilane groups. Doxorubicin (DOX), an anti-cancer drug, was loaded into the pores of a GA-attached Fe3O4@MSNs due to both its anti-cancer properties and to allow for the fluorescent visualization of the nanocarriers. The selective and efficient mitochondria-targeting ability of a DOX-loaded GA-Fe3O4@MSNs (DOX/GA-Fe3O4@MSNs) was demonstrated by a co-localization study, transmission electron microscopy, and a fluorometric analysis on isolated mitochondria. It was found that the DOX/GA-Fe3O4@MSNs selectively accumulated into mitochondria within only five minutes; to the best of our knowledge, this is the shortest accumulation time reported for mitochondria targeting systems. Moreover, 2.6 times higher amount of DOX was accumulated in mitochondria by DOX/GA-Fe3O4@MSNs than by DOX/TPP-Fe3O4@MSNs. A cell viability assay indicated that the DOX/GA-Fe3O4@MSNs have high cytotoxicity to cancer cells, whereas the GA-Fe3O4@MSNs without DOX are non-cytotoxic; this indicates that the DOX/GA-Fe3O4@MSNs have great potential for use as biocompatible and effective mitochondria-targeting nanocarriers for cancer therapy.</P>

Development of ⁶⁸Ga-human serum albumin as a PET imaging agent for diagnosis of acute inflammation

Lee, Ji Youn,Kim, Hoyoung,Lee, Boeun,Kim, Young Ju,Lee, Yun-Sang,Jeong, Jae Min 대한방사성의약품학회 2015 Journal of radiopharmaceuticals and molecular prob Vol.1 No.2

Human serum albumin (HSA) has potential for diagnosis and therapy in clinical setting. The purpose of experiments was to develop and evaluate $^{68}Ga$-HSA as a PET agent for diagnosis of acute inflammation. NOTA-HSA was synthesized by conjugating 2-(p-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid to HSA in 0.1 M sodium carbonate buffer (pH 9.5) and then purified using a PD-10 size-exclusion column. NOTA-HSA was labeled with $^{68}Ga$ at room temperature for 10 min, and 8.4% sodium hydrogen carbonate buffer was added for neutralization. $^{68}Ga$-NOTA-HSA was purified using alumina N plus light cartridge and $0.22{\mu}m$ syringe filter. Labeling efficiency and radiochemical purity were determined by ITLC-SG with 0.1 M citric acid. Biodistribution study was performed in a male BALB/c mice model of Carrageenan-induced acute inflammation. Animal PET study was performed in acute inflammation mice model after tail vein injection of $^{68}Ga$-HSA. This radiotracer showed high labeling efficiency (>99%) around pH 7. Biodistribution study showed higher inflamed footpad uptake than control footpad uptake. Animal PET study revealed 2 times higher uptake on inflamed footpad compared to control footpad. In these experiments, we developed $^{68}Ga$-HSA for acute inflammation PET imaging and evaluated it in a mouse disease model. The results demonstrated that $^{68}Ga$-HSA has potential as a PET imaging agent for diagnosis of acute inflammation.

Translocation of residual ethoprophos and tricyclazole from soil to spinach

Yuan Xiu,Lee Junghak,Han Heeju,Ju Boeun,Park Eunyoung,Shin Yongho,Lee Jonghwa,Kim Jeong-Han 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.3

The dissipation of ethoprophos and tricyclazole in soil and their translocation tendency to spinach were investigated. Prior to field trials, the analytical method for the determination of these pesticide residues was optimized and validated on soil and spinach. The field trial was conducted under greenhouse conditions for two different pretreatment periods with the pesticides. After treating with pesticides 30 (PBI-30) and 60 days (PBI-60) before seeding, soil samples were collected on different days for the dissipation study of soil. Spinach samples were harvested from the soil, and 50% and 100% mature spinach samples were collected. The initial amounts of ethoprophos residue in the PBI-60 and PBI-30 soils were 0.21 and 2.74 mg/kg, respectively, and these both decreased to less than 0.01 mg/kg on the day of spinach harvest. Similar initial residues of tricyclazole were observed in the PBI-60 (0.87 mg/kg) and PBI-30 soils (0.84 mg/kg), and these decreased to 0.44 and 0.34 mg/kg, respectively. The half-lives of ethoprophos in the soils were calculated as 7.6 and 4.8 days, respectively, while relatively long half-lives of 36.5 and 77.0 days were calculated for tricyclazole. According to the pesticide residue amounts in the spinach, the translocation rate from the soil to the spinach was determined. In the case of ethoprophos, the residual amount was already rapidly degraded in the soil, and the translocation rate could not be confirmed. On the other hand, for tricyclazole, it was confirmed that 1.19 to 1.61% of the residual amount in soil was transferred to spinach. According to these results, safe management guidelines for tricyclazole in soil were suggested considering the maximum residue limit on spinach.

Donor-π-Acceptor Type Diphenylaminothiophenyl Anthracene-mediated Organic Photosensitizers for Dye-sensitized Solar Cells

Heo, Dong Uk,Kim, Sun Jae,Yoo, Beom Jin,Kim, Boeun,Ko, Min Jae,Cho, Min Ju,Choi, Dong Hoon Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.4

Two new metal-free organic dyes bridged by anthracene-mediated ${\pi}$-conjugated moieties were successfully synthesized for use in a dye-sensitized solar cell (DSSC). A N,N-diphenylthiophen-2-amine unit in these dyes acts as an electron donor, while a (E)-2-cyano-3-(thiophen-2-yl)acrylic acid group acts as an electron acceptor and an anchoring group to the $TiO_2$ electrode. The photovoltaic properties of (E)-2-cyano-3-(5-((10-(5-(diphenylamino)thiophen-2-yl)anthracen-9-yl)ethynyl)thiophen-2-yl)acrylic acid (DPATAT) and (E)-2-cyano-3-(5'-((10-(5-(diphenylamino)thiophen-2-yl)anthracen-9-yl)ethynyl)-2,2'-bithiophen-5-yl)acrylic acid (DPATABT) were investigated to identify the effect of conjugation length between electron donor and acceptor on the DSSC performance. By introducing an anthracene moiety into the dye structure, together with a triple bond and thiophene moieties for fine-tuning of molecular configurations and for broadening the absorption spectra, the short-circuit photocurrent densities ($J_{sc}$), and open-circuit photovoltages ($V_{oc}$) of DSSCs were improved. The improvement of $J_{sc}$ in DSSC made of DPATABT might be attributed to much broader absorption spectrum and higher molecular extinction coefficient (${\varepsilon}$) in the visible wavelength range. The DPATABT-based DSSC showed the highest power conversion efficiency (PCE) of 3.34% (${\eta}_{max}$ = 3.70%) under AM 1.5 illumination ($100mWcm^{-2}$) in a photoactive area of $0.41cm^2$, with the $J_{sc}$ of $7.89mAcm^{-2}$, the $V_{oc}$ of 0.59 V, and the fill factor (FF) of 72%. In brief, the solar cell performance with DPATABT was found to be better than that of DPATAT-based DSSC.

GC-MS/MS를 활용한 소고기 및 우유 중 잔류농약 다성분 동시분석법 확립

한희주 ( Heeju Han ),이정학 ( Junghak Lee ),원수 ( Xiu Yuan ),박은영 ( Eunyoung Park ),주보은 ( Boeun Ju ),신용호 ( Yongho Shin ),김정한 ( Jeong-han Kim ) 한국환경농학회 2020 한국환경농학회 학술대회집 Vol.2020 No.-

축산물에서 잔류농약이 검출되는 사례가 발생하면서 식품의 안전성에 대한 불안감이 확산되고 있고 농약허용물질 관리제도(PLS)의 도입이 확정되면서 신뢰할 수 있는 잔류농약 분석법의 필요성이 제고되고 있는 실정이다. 이에, 본 연구는 육류 중 국내 섭취량이 높으면서 포화지방성분이 많아 분석이 어려울 것으로 예상되는 소고기와 그 부산물인 우유를 대상으로 GC 분석이 가능한 농약의 신속 정확한 다성분 분석법을 확립해보고자 하였다. 해외 잔류허용기준 (maximum residue limit, MRLs) 이 존재하는 농약 중 QuEChERS로 전처리가 가능하고 GC-MS/MS로 분석이 가능한 성분을 선정하여 이번 연구를 진행하였다. GC-MS/MS는 Shimadzu GCMS-8050 를 사용하였고 분석 정량한계 (MLOQ, method limit of quantitation)는 0.01 mg/kg이었으며 검량 범위 3-50 ug/mL에서 검량선 상관계수(r2)는 r2>0.98이었다. 분석기기 상의 오차를 보정하기 위해 내부표준물질로 triphenylphosphate (TPP)를 사용하였고, 회수율을 계산하기 위하여 MLOQ, 10MLOQ, 50MLOQ 수준으로 표준용액을 처리하였다. 전처리 조건을 최적화하기 위하여 추출용매와 정제법을 다르게 하여 비교하였다. 추출용매는 0.1% formic acid를 첨가한 acetonitrile 용매와 이에 ethyl acetate를 첨가한 용매를 비교하였다. 작물에 비해서 지방이 많은 축산물의 특성을 고려하여 총 5종류의 정제법을 비교하였다 [dSPE 3종류(C18-dSPE, 2-SEP, Z-SEP plus)와 SPE 2종류(Prime HLB, Phree)]. 113가지 농약성분 중 회수율이 70~120%를 만족하는 성분들의 개수를 비교하였고, 결과적으로 추출용매의 경우, 소고기는 0.1% formic acid와 50% ethyl acetate를 첨가한 acetonitrile 용매가, 우유는 0.1% formic acid를 첨가한 acetonitrile 용매가 효과적으로 확인되었다. 정제의 경우, Z-SEP을 이용하여 정제하는 조건에서 안정적인 경향성을 보이고 있어 동시분석에 용이할 것으로 판단되었다. 본 연구는 해당 농약들을 소고기와 우유에서 동시 정량분석이 가능해서 소고기와 우유에 대한 해당 잔류농약 검사 및 MRLs을 설정하는 데 기여할 수 있을 것으로 기대된다.

Neurodevelopment in Early Childhood Affected by Prenatal Lead Exposure and Iron Intake

Shah-Kulkarni, Surabhi,Ha, Mina,Kim, Byung-Mi,Kim, Eunjeong,Hong, Yun-Chul,Park, Hyesook,Kim, Yangho,Kim, Bung-Nyun,Chang, Namsoo,Oh, Se-Young,Kim, Young Ju,Lee, Boeun,Ha, Eun-Hee Wolters Kluwer Health 2016 Medicine Vol.95 No.4

<▼1><P>Supplemental Digital Content is available in the text</P></▼1><▼2><P><B>Abstract</B></P><P>No safe threshold level of lead exposure in children has been recognized. Also, the information on shielding effect of maternal dietary iron intake during pregnancy on the adverse effects of prenatal lead exposure on children's postnatal neurocognitive development is very limited. We examined the association of prenatal lead exposure and neurodevelopment in children at 6, 12, 24, and 36 months and the protective action of maternal dietary iron intake against the impact of lead exposure.</P><P>The study participants comprise 965 pregnant women and their subsequent offspring of the total participants enrolled in the Mothers and Children's environmental health study: a prospective birth cohort study. Generalized linear model and linear mixed model analysis were performed to analyze the effect of prenatal lead exposure and mother's dietary iron intake on children's cognitive development at 6, 12, 24, and 36 months.</P><P>Maternal late pregnancy lead was marginally associated with deficits in mental development index (MDI) of children at 6 months. Mothers having less than 75th percentile of dietary iron intake during pregnancy showed significant increase in the harmful effect of late pregnancy lead exposure on MDI at 6 months. Linear mixed model analyses showed the significant detrimental effect of prenatal lead exposure in late pregnancy on cognitive development up to 36 months in children of mothers having less dietary iron intake during pregnancy.</P><P>Thus, our findings imply importance to reduce prenatal lead exposure and have adequate iron intake for better neurodevelopment in children.</P></▼2>