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Various Three-Dimensional Culture Methods and Cell Types for Exosome Production
Lee Dong-Hyun,Yun Dae Won,Kim Yeong Hwan,임광범,Hyun Jiyu,Park Hyun Su,방석호,Choi Sang Hyoun 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.4
Cell-based therapies have been used as promising treatments for several untreatable diseases. However, cell-based therapies have side effects such as tumorigenesis and immune responses. To overcome these side effects, therapeutic effects of exosomes have been researched as replacements for cell-based therapies. In addition, exosomes reduced the risk that can be induced by cell-based therapies. Exosomes contain biomolecules such as proteins, lipids, and nucleic acids that play an essential role in cell–cell and cell–matrix interactions during biological processes. Since the introduction of exosomes, those have been proven perpetually as one of the most effective and therapeutic methods for incurable diseases. Much research has been conducted to enhance the properties of exosomes, including immune regulation, tissue repair, and regeneration. However, yield rate of exosomes is the critical obstacle that should be overcome for practical cell-free therapy. Three-dimensional (3D) culture methods are introduced as a breakthrough to get higher production yields of exosomes. For example, hanging drop and microwell were well known 3D culture methods and easy to use without invasiveness. However, these methods have limitation in mass production of exosomes. Therefore, a scaffold, spinner flask, and fiber bioreactor were introduced for mass production of exosomes isolated from various cell types. Furthermore, exosomes treatments derived from 3D cultured cells showed enhanced cell proliferation, angiogenesis, and immunosuppressive properties. This review provides therapeutic applications of exosomes using 3D culture methods.
주현준(Hyun-Jun Joo),김정원(Jungwon Kim),조대연(Daeyeon Cho),정지유(Jiyu Jeong),고남선(Namsun Ko),김은경(Eunkyung Gim) 중앙대학교 한국인적자원개발전략연구소 2021 역량개발학습연구 Vol.16 No.1
본 연구의 주요 목적은 A교육대학교의 조직현황과 특성을 분석하고, 조직역량과 핵심 요소를 도출하여 이를 측정할 수 있는 타당성과 신뢰성 높은 조직역량 진단 도구 개발을 목적으로 한다. 문헌연구, 초점집단면접, 델파이, 설문조사를 통해서 조직역량의 정의와 구성요인을 확인하였고, 진단 도구를 검증하였다. 설문조사는 교육대학교 학교구성원을 대상으로 설문을 실시하였으며, 회수된 유효 설문 100부를 활용하여 기술통계분석, 예비 문항 상관관계 분석, 구성타당도, 신뢰도 검증, 판별타당도 검증, 준거타당도 검증을 차례로 실시하였다. 연구 결과, 조직역량의 구성요인은 공유가치와 전략, 구조와 제도, 구성원과 기술, 리더십, 관계성의 5개 요인이며, 델파이 조사를 통해 총 39개의 예비문항을 도출하였다. 구성타당도 검증에서 탐색적 요인분석을 통해 총 26개 문항이 도출되었으며, 확인적 요인분석 과정을 통해 5개 요인의 26개 문항을 확정하였다. 이어 준거타당도 검증을 통해 정서적 몰입과의 통계적으로 정적인 영향 관계를 검증했으며(ß= .842, p< .001), 정서적 몰입의 선행변인으로써 조직역량을 확인하였다. 마지막으로 신뢰도 검증을 위해 Cronbach α값을 활용하였으며, 전체 조직역량 진단 도구의 Cronbach α값은 .965, 5개 요인 역시 각 각 .8 이상의 높은 신뢰도를 보이는 것으로 조사되었다. 이러한 결과를 바탕으로 향후 조직역량 진단 도구를 활용한 종단적 연구와 학교구성원 지원 프로그램 제공이 필요함을 제안하였으며, 주기적으로 실시되는 교원양성기관 역량진단과 같은 평가를 보안하는 대안으로서 활용 가능함을 밝혔다. The purpose of this study is to analyze the current status and characteristics of ‘A’ National University of Education and to develop a valid and reliable diagnostic tool for measuring organizational competency and other key factors. The definition and factors of organizational competency were identified through literature reviews, focus group interviews, a Delphi survey, and a questionnaire. The survey was conducted targeting the faculty and staff of the University of Education, and all 100 effective questionnaires were used to analyze technical statistics, correlation, reliability, and validity. As a result, a total of 26 questions from five factors were confirmed, and the five factors of organizational competency are shared values and strategies, structure and system, staff and skills, leadership, and relationship. Subsequently, examining the convergent validity the tool, it was found to be suitable for measuring emotional engagement (ß= .842, p<.001). Finally, the Cronbach α value of the diagnostic tool was found to be .965. Based on these results, the study proposed the need for long-term research using the organizational competency diagnostic tool and providing support programs for staff members at the university.
방사성물질 측정망 현황 및 하천·호소 내 인공방사성물질 (<SUP>134</SUP>Cs, <SUP>137</SUP>Cs, <SUP>131</SUP>I) 조사
김지유(Jiyu Kim),정현지(Hyun-ji Jung),안미정(Mijeong An),홍정기(Jung-Ki Hong),강태구(Taegu Kang),강태우(Tae-Woo Kang),조윤해(Yoon-Hae Cho),한영운(Yeong-Un Han),설빛나(Bitna Seol),김완석(Wansuk Kim),김경현(Kyunghyun Kim) 한국분석과학회 2015 분석과학 Vol.28 No.6
Kim, Hyun Jin,Kim, Jiyu,Cho, Seung Hwan,Chang, Sukbok American Chemical Society 2011 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.133 No.41
<P>A new synthetic approach toward intermolecular oxidative C–N bond formation of arenes has been developed under transition-metal-free conditions. Complete control of chemoselectivity between aryl sp<SUP>2</SUP> and benzylic sp<SUP>3</SUP> C–H bond imidation was achieved by the choice of nitrogen sources, representatively being phthalimide and dibenzenesulfonimide, respectively.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2011/jacsat.2011.133.issue-41/ja207296y/production/images/medium/ja-2011-07296y_0012.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja207296y'>ACS Electronic Supporting Info</A></P>
Kim, Jiyu,Park, Sung-Woo,Baik, Mu-Hyun,Chang, Sukbok American Chemical Society 2015 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.137 No.42
<P>A complete switch in the Cp*Ir(III)-catalyzed paths between C-H olefination and hydroarylation was found to be crucially dependent on the type of directing groups. This dichotomy in product distribution was correlated to the efficiency in attaining syn-coplanarity of olefin-inserted 7-membered iridacycles. Theoretical studies support our hypothesis that the degree of flexibility of this key intermediate modulates the beta-H elimination, which ultimately affords the observed chemoselectivity.</P>
Im Jisoo,Hyun Jiyu,Kim Sung-Won,방석호 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.1
BACKGROUND: Enhancing blood flow and cell proliferation in the hair dermis is critical for treating hair loss. This study was designed to aid the development of alternative and effective solutions to overcome alopecia. Specifically, we examined the effects of Morus alba. L root extract (MARE, which has been used in traditional medicine as a stimulant for hair proliferation) on dermal fibroblasts and other cell types found in the epidermis. METHODS: We first optimized the concentration of MARE that could be used to treat human dermal fibroblasts (HDFs) without causing cytotoxicity. After optimization, we focused on the effect of MARE on HDFs since these cells secrete paracrine factors related to cell proliferation and angiogenesis that affect hair growth. Conditioned medium (CM) derived from MARE-treated HDFs (MARE HDF-CM) was used to treat human umbilical vein endothelial cells (HUVECs) and hair follicle dermal papilla cells (HFDPCs). RESULTS: Concentrations of MARE up to 20 wt% increased the expression of proliferative and anti-apoptotic genes in HDFs. MARE HDF-CM significantly improved the tubular structure formation and migration capacity of HUVECs. Additionally, MARE HDF-CM treatment upregulated the expression of hair growth-related genes in HFDPCs. CM collected from MARE-treated HDFs promoted the proliferation of HFDPCs and the secretion of angiogenic paracrine factors from these cells. CONCLUSION: Since it can stimulate the secretion of pro-proliferative and pro-angiogenic paracrine factors from HDFs, MARE has therapeutic potential as a hair loss preventative.
JIANG PENGFEI,Hyun Jiyu,박현수,방석호,김우식,유태경 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.9
An organic surfactant/stabilizer plays a critical role in synthesizing small and well-dispersed nanoparticles with large catalytic surface areas. However, a stabilizer typically interferes with the catalytic reaction by forming organic layers on the surface of nanoparticles. Here, we report that the role of a stabilizer can be replaced by the periodic and uniform fluid shear of Taylor vortex flow. Small, well-dispersed PdAg alloy nanoparticles on carbon were synthesized in the continuous Couette-Taylor (CT) reactor in the absence of a stabilizer. The synthesized PdAg nanoparticles (Pd64Ag36/C) showed two times higher mass activity than commercial Pd/C and good stability (retained 33% after 3,600 s) toward the electrochemical ethanol oxidation reaction due to their clean surface and alloy structure.
LIU CUN,최진영,Hyun Jiyu,방석호,유태경 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.11
To synthesize nanocomposites (NCs) comprising two different materials, a multi-step synthetic method is generally required. In this work, we report the synthesis of a heterometallic nanostructure that can be synthesized using a simple antisolvent crystallization-based method. Cu/CoO NCs composed of Cu nanoparticles and CoO nanosheets were formed by reducing mixed salt particles containing both Cu and Co precursors. The synthesized Cu/CoO NCs exhibit higher sensitivity and increased stability compared to conventional catalysts when used for the electrochemical detection of hydrogen peroxide (H2O2).