Classification of soybeans from different habitats based on metabolomic–transcriptomic integration

Wang Jinghui,Zheng Qiyou,Wang Chenxu,Zhou Ao 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-

Soybeans are a significant agricultural product in China, with certain geographical locations often yielding higher quality, and thus more expensive, soybean crops. In this study, metabolomics and transcriptomics analyses were con ducted on soybean samples from nine regions in Heilongjiang and Liaoning Provinces using untargeted liquid chro matography–mass spectrometry (LC–MS) and Illumina sequencing technologies. The primary objective was to devise an effective and unbiased method for determining the geographical origin of each soybean variety to mitigate potential fraudulent practices. Through multidimensional and unidimensional analyses, successful identification of dif ferentially expressed metabolites (DEMs) and differentially expressed genes (DEGs) was achieved, yielding statisti cally significant outcomes. Integration of the metabolomics and transcriptomics datasets facilitated the construction of a correlation network model capable of distinguishing soybeans originating from different geographical locations, leading to the identification of significant biomarkers exemplifying noteworthy distinctions. To validate the feasibil ity of this method in practical applications, partial least squares discriminant analysis was employed to differentiate soybean samples from the nine regions. The results convincingly showcased the applicability and reliability of this approach in accurately pinpointing the geographical origin of soybeans. Distinguishing itself from prior research in soybean traceability, this study incorporates an integrated analysis of metabolomics and transcriptomics data, thereby unveiling biomarkers that offer a more precise differentiation of soybean traits across distinct regions, thereby bridging a critical research gap within the soybean traceability domain. This innovative dual-data integration analysis methodology is poised to enhance the accuracy of soybean traceability tools and lay a new foundation for future agricultural product identification research.

Ginsenoside Re inhibits myocardial fibrosis by regulating miR-489/myd88/NF-κB pathway

Jinghui Sun,Ru Wang,Tiantian Chao,Jun Peng,Chenglong Wang,Keji Chen The Korean Society of Ginseng 2023 Journal of Ginseng Research Vol.47 No.2

Background: Myocardial fibrosis (MF) is an advanced pathological manifestation of many cardiovascular diseases, which can induce heart failure and malignant arrhythmias. However, the current treatment of MF lacks specific drugs. Ginsenoside Re has anti-MF effect in rat, but its mechanism is still not clear. Therefore, we investigated the anti-MF effect of ginsenoside Re by constructing mouse acute myocardial infarction (AMI) model and AngII induced cardiac fibroblasts (CFs) model. Methods: The anti-MF effect of miR-489 was investigated by transfection of miR-489 mimic and inhibitor in CFs. Effect of ginsenoside Re on MF and its related mechanisms were investigated by ultrasonographic, ELISA, histopathologic staining, transwell test, immunofluorescence, Western blot and qPCR in the mouse model of AMI and the AngII-induced CFs model. Results: MiR-489 decreased the expression of α-SMA, collagenI, collagen III and myd88, and inhibited the phosphorylation of NF-κB p65 in normal CFs and CFs treated with AngII. Ginsenoside Re could improve cardiac function, inhibit collagen deposition and CFs migration, promote the transcription of miR-489, and reduce the expression of myd88 and the phosphorylation of NF-κB p65. Conclusion: MiR-489 can effectively inhibit the pathological process of MF, and the mechanism is at least partly related to the regulation of myd88/NF-κB pathway. Ginsenoside Re can ameliorate AMI and AngII induced MF, and the mechanism is at least partially related to the regulation of miR-489/myd88/NF-κB signaling pathway. Therefore, miR-489 may be a potential target of anti-MF and ginsenoside Re may be an effective drug for the treatment of MF.

Corrigendum Algorithm for Gesture Recognition Based on Multiple Information Fusion and Kinect

Jinghui Wang,Wenqun Cao,Mingzhi Niu 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.10

Gesture recognition is an important and challenging task in the field of computer vision. Starting from the 3D shape of coding gestures, it puts forward a new kind of gesture recognition framework based on depth image. It extracts the space characteristics of a variety of 3D point cloud based on Kinect, including local principal components analysis on point cloud to get the histogram of main component, gradient direction histogram based on local depth difference and depth distribution histogram of local point cloud. Principal component histogram and gradient direction histogram effectively coding the local shape of gestures, depth distribution histogram compensates the loss of the shaping descriptor information. Through preliminary training of random forest classifier to filter the characteristics, and characteristics with less influence on classification results are removed, thus the computational costs are reduced. The filtered characteristics are used for training of random forest classifier again to classify gestures. Experiment is carried on two large-scale gesture data sets, for more difficult ASL dataset, the proposed method has improved the recognition rate of 3.6% then the best previous algorithm.

Robust Gesture Recognition with Kinect Data Acquisition

Jinghui Wang,Mingzhi Niu 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.10

To realize the gesture recognition of high precision ratio, the gesture recognition method of multi-model data fusion based on Kinect depth image is proposed, to implement the automatic splicing of models. First of all, the feature package model uses the speeded up robust feature (SURF) algorithm to replace the scale invariant feature transform (SIFT) algorithm to extract features, improve the real-time performance. Secondly, Hu moment is introduced to describe the global gesture features, further improving the recognition rate, the ray casting is used finally, and the obtained coordinate information is used to solve the rigid transformation between two point cloud models. Finally, the proposed data fusion method is verified through two experiments, the algorithm in this paper is better than the traditional support vector machine (SVM) method both in real time performance and recognition rate, and obtains better model splicing effect.

Streptococcus pneumoniae aminopeptidase N contributes to bacterial virulence and elicits a strong innate immune response through MAPK and PI3K/AKT signaling

Ling Wang,Xuemei Zhang,Guangying Wu,Yuhong Qi,Jinghui Zhang,Jing Yang,Hong Wang,Wenchun Xu 한국미생물학회 2020 The journal of microbiology Vol.58 No.4

Streptococcus pneumoniae is a Gram-positive pathogen with high morbidity and mortality globally but some of its pathogenesis remains unknown. Previous research has provided evidence that aminopeptidase N (PepN) is most likely a virulence factor of S. pneumoniae. However, its role in S. pneumoniae virulence and its interaction with the host remains to be confirmed. We generated a pepN gene deficient mutant strain and found that its virulence for mice was significantly attenuated as were in vitro adhesion and invasion of host cells. The PepN protein could induce a strong innate immune response in vivo and in vitro and induced secretion of IL-6 and TNF-α by primary peritoneal macrophages via the rapid phosphorylation of MAPK and PI3K/AKT signaling pathways and this was confirmed using specific pathway inhibitors. In conclusion, PepN is a novel virulence factor that is essential for the virulence of S. pneumoniae and induces host innate immunity via MAPK and PI3K/AKT signaling.

Protective Effect of Schisandra chinensis Polysaccharides Against the Immunological Liver Injury in Mice Based on Nrf2/ARE and TLR4/NF-κB Signaling Pathway

Yingying Shan,Bin Jiang,Jiahui Yu,Jiaye Wang,Xiaoli Wang,He Li,Chunmei Wang,JianGuang Chen,Jinghui Sun 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.9

We have previously demonstrated the hepatoprotective effect of Schisandra chinensis polysaccharides (SCP) against the liver injury induced by alcohol, high-fat diet, and carbon tetrachloride in mice. In this study, we investigated the effect of SCP against the immunological liver injury induced by concanavalin A (Con A) in mice. The results showed that SCP could significantly reduce the level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in the serum of mice with immunological liver injury. SCP could significantly decrease the content of malondialdehyde (MDA) and nitric oxide (NO) and increase the activity of superoxide dismutase (SOD) and glutathione (GSH) in the liver tissue. SCP could significantly increase the number of CD4+ and decrease the number of CD8+ in the peripheral blood, and elevate the ratio of CD4+/CD8+. SCP could significantly downregulate the expression of Kelch-like ECH-associated protein 1 (Keap1) and upregulate the expression of nuclear factor-erythroid 2-related factor2 (Nrf2) and downstream gene heme oxygenase-1 (HO-1), and downregulate the expression of toll-like receptor 4 (TLR4), myeloid differentiation primary response gene 88 (MyD88), and nuclear factor-kappa B (NF-κB) proteins. This study indicates that SCP can reduce the release of a large number of inflammatory factors to inhibit the oxidative stress in mice with the immunological liver injury induced by Con A, and its mechanism is closely related to the regulation of Nrf2/antioxidant response element and TLR4/NF-κB signaling pathways.

Selective deposition of Au-Pt alloy nanoparticles on ellipsoidal zirconium titanium oxides for reduction of 4-nitrophenol

Zewu Zhang,Jinghui Zhang,Guangqing Liu,Mengwei Xue,Zhangzhong Wang,Xiaohai Bu,Qiong Wu,Xuejuan Zhao 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.9

Au-Pt alloy nanoparticles that are selectively anchored on TiO2 surface of the ellipsoidal zirconium titanium composite oxides were successfully prepared by a facile two-step method: prefabricated binary composite oxides on the ellipsoidal Fe2O3@SiO2 by a versatile cooperative template-directed coating method, and then in situ formation of Au-Pt alloy NPs with Sn2+ as the reduction agent. The alloy catalysts were characterized by X-ray diffraction, transmission electron microscopy and X-ray photoelectron spectroscopy. The result suggested that highly dispersive and ultrafine Au-Pt alloy nanoparticles were deposited onto TiO2 surface of the binary oxides solely. The particle size of nanoalloys was closely related to the ratio of Zr : Ti in the composite oxides shell. Increasing the content of Zr element led to a growth in the size of alloy nanoparticles. When used as catalysts for the reduction of 4-nitrophenol, the prepared supported alloyed catalysts exhibited high catalytic activity, and the sample could be easily recycled without a significant decrease of the catalytic activity.

Effects of Lactobacillus helveticus Fermentation on the Ca2+ Release and Antioxidative Properties of Sheep Bone Hydrolysate

Keguang Han,Jing Cao,Jinghui Wang,Jing Chen,Kai Yuan,Fengping Pang,Shaopeng Gu,Nairui Huo 한국축산식품학회 2018 한국축산식품학회지 Vol.38 No.6

Both the calcium and collagen in bone powder are hard to be absorbed by the body. Although enzymatic hydrolysis by protease increased the bio-availability of bone powder, it was a meaningful try to further increase Ca2+ release, oligopeptide formation and antioxidant activity of the sheep bone hydrolysate (SBH) by lactic acid bacteria (LAB) fermentation. Lactobacillus helveticus was selected as the starter for its highest protease-producing ability among 5 tested LAB strains. The content of liberated Ca2+ was measured as the responsive value in the response surface methodology (RSM) for optimizing the fermenting parameters. When SBH (adjusted to pH 6.1) supplemented with 1.0% glucose was inoculated 3.0% L. helveticus and incubated for 29.4 h at 36℃, Ca2+ content in the fermented SBH significantly increased (p<0.01), and so did the degree of hydrolysis and the obtaining rate of oligopeptide. The viable counts of L. helveticus reached to 1.1×1010 CFU/mL. Results of Pearson correlation analysis demonstrated that LAB viable counts, Ca2+ levels, obtaining rates of oligopeptide and the yield of polypeptide were positively correlated with each other (p<0.01). The abilities of SBH to scavenge the free radicals of DPPH, OH and ABTS were also markedly enhanced after fermentation. In conclusion, L. helveticus fermentation can further boost the release of free Ca2+ and oligopeptide, enhance the antioxidant ability of SBH. The L. helveticus fermented SBH can be developed as a novel functional dietary supplement product.

Free vibration analysis of cracked Timoshenko beams carrying spring-mass systems

Tan, Guojin,Shan, Jinghui,Wu, Chunli,Wang, Wensheng Techno-Press 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.63 No.4

In this paper, an analytical approach is proposed for determining vibration characteristics of cracked non-uniform continuous Timoshenko beam carrying an arbitrary number of spring-mass systems. This method is based on the Timoshenko beam theory, transfer matrix method and numerical assembly method to obtain natural frequencies and mode shapes. Firstly, the beam is considered to be divided into several segments by spring-mass systems and support points, and four undetermined coefficients of vibration modal function are contained in each sub-segment. The undetermined coefficient matrices at spring-mass systems and pinned supports are obtained by using equilibrium and continuity conditions. Then, the overall matrix of undetermined coefficients for the whole vibration system is obtained by the numerical assembly technique. The natural frequencies and mode shapes of a cracked non-uniform continuous Timoshenko beam carrying an arbitrary number of spring-mass systems are obtained from the overall matrix combined with half-interval method and Runge-Kutta method. Finally, two numerical examples are used to verify the validity and reliability of this method, and the effects of cracks on the transverse vibration mode shapes and the rotational mode shapes are compared. The influences of the crack location, depth, position of spring-mass system and other parameters on natural frequencies of non-uniform continuous Timoshenko beam are discussed.

Chemical Composition and Antimigraine Activity of Essential Oil of Angelicae dahuricae Radix

Jingbo Sun,He Li,Jinghui Sun,Huimin Liu,JianGuang Chen,Chunmei Wang 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.8

The aim of this study was to explore the chemical composition and the effect of essential oil of Angelicae dahuricae radix on a nitroglycerin (NTG)-induced rat model of migraine. The CO2 supercritical fluid extraction method was optimized for the extraction of essential oil of A. dahuricae radix (EOAD) and its chemical composition was determined. The migraine model was established by subcutaneous injection of NTG (10 mg/kg) 1 h after the last administration of EOAD. The therapeutic effect of EOAD and its underlying mechanism were assessed by monitoring behavioral changes, levels of nitric oxide (NO) in serum and brain tissues, plasma levels of calcitonin gene-related peptide (CGRP) and endothelin (ET), and ET/NO ratio. The optimal conditions for CO2 supercritical fluid extraction of EOAD, as determined by orthogonal test [L9(34)], were as follows: 2 h extraction time, 20 MPa pressure, 40°C temperature, and 30 mesh. The yield of EOAD was 1.8%. On gas chromatography–mass spectrometry, 45 peaks were found in EOAD, and 22 compounds were identified and quantified. The main constituents of EOAD were 1-dodecanol (13.71%), elemene (7.54%), palmitic acid ethyl ester (7.32%), α-pinene (6.25%), and 1-pentadecanol (6.08%). Compared with rat migraine model controls, EOAD (35, 70, and 140 mg/kg) significantly reduced the number of head shaking, head scratching, and hind leg shooting events, decreased serum and brain NO levels, decreased plasma CGRP, and increased ET levels in rats. ET/NO ratio was elevated to 28.68 in the EOAD high-dose group. EOAD ameliorates NTG-induced migraine in rats likely by modulating the levels of vasoactive substances.