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Jing Lai,Fang Yang,Wenwen Zhang,Yanru Wang,Jing Xu,Wei Song,Guichun Huang,Jun Gu,Xiaoxiang Guan 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.8
The p73 gene contains an extrinsic P1 promoter and an intrinsic P2 promoter, controlling the transcription of the pro-apoptotic TAp73 isoform and the anti-apoptotic ΔΝp73 isoform, respectively. The DNA methylation status of both promoters act equally in the epigenetic transcriptional regulation of their relevant isoforms. The aim of this study was to analyze the different effects of these p73 isoforms in 5-aza-2'-deoxycytidine (5-aza-dC)-induced apoptosis in breast cancer cells. We investigated the effects of the DNA demethylation agent, 5-aza-dC, on the T-47D breast cancer cell line, and evaluated the methylation status of the p73 promoters and expression of TAp73 and ΔNp73. Furthermore, we assessed the expression of p53 and p73 isoforms in 5-aza-dC-treated T-47D cells and p53 knockout cells. 5-aza-dC induced significant anti-tumor effects in T-47D cells, including inhibition of cell viability, G1 phase arrest and apoptosis. This was associated with p73 promoter demethylation and a concomitant increase in TAp73 mRNA and protein expression. In contrast, the methylation status of promoter P2 was not associated with ΔNp73 mRNA or protein levels. Furthermore, demethylation of P2 failed to inhibit the expression of ΔNp73 with 5-aza-dC in the p53 knockdown cell model. Our study suggests that demethylation of the P1 and P2 promoters has opposite effects on the expression of p73 isoforms, namely up-regulation of TAp73 and down-regulation of ΔΝp73. We also demonstrate that p53 likely contributes to 5-aza-dC-induced ΔNp73 transcriptional inactivation in breast cancer cells.
Lai, Jing,Yang, Fang,Zhang, Wenwen,Wang, Yanru,Xu, Jing,Song, Wei,Huang, Guichun,Gu, Jun,Guan, Xiaoxiang Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.8
The p73 gene contains an extrinsic P1 promoter and an intrinsic P2 promoter, controlling the transcription of the pro-apoptotic TAp73 isoform and the anti-apoptotic ${\Delta}Np73$ isoform, respectively. The DNA methylation status of both promoters act equally in the epigenetic transcriptional regulation of their relevant isoforms. The aim of this study was to analyze the different effects of these p73 isoforms in 5-aza-2'-deoxycytidine (5-aza-dC)-induced apoptosis in breast cancer cells. We investigated the effects of the DNA demethylation agent, 5-aza-dC, on the T-47D breast cancer cell line, and evaluated the methylation status of the p73 promoters and expression of TAp73 and ${\Delta}Np73$. Furthermore, we assessed the expression of p53 and p73 isoforms in 5-aza-dC-treated T-47D cells and p53 knockout cells. 5-aza-dC induced significant anti-tumor effects in T-47D cells, including inhibition of cell viability, G1 phase arrest and apoptosis. This was associated with p73 promoter demethylation and a concomitant increase in TAp73 mRNA and protein expression. In contrast, the methylation status of promoter P2 was not associated with ${\Delta}Np73$ mRNA or protein levels. Furthermore, demethylation of P2 failed to inhibit the expression of ${\Delta}Np73$ with 5-aza-dC in the p53 knockdown cell model. Our study suggests that demethylation of the P1 and P2 promoters has opposite effects on the expression of p73 isoforms, namely up-regulation of TAp73 and down-regulation of ${\Delta}Np73$. We also demonstrate that p53 likely contributes to 5-aza-dC-induced ${\Delta}Np73$ transcriptional inactivation in breast cancer cells.
The Gender-Sensitive Social Risk Factors for Internet Addiction in College Undergraduate Students
Xia Lin,Jing-yan Gu,Wan-jun Guo,Ya-jing Meng,Hui-yao Wang,Xiao-jing Li,Wei Deng,Lian-sheng Zhao,Xiao-hong Ma,Ming-li Li,Ting Chen,S,K,Cheng,Tao Li 대한신경정신의학회 2021 PSYCHIATRY INVESTIGATION Vol.18 No.7
Objective The current study aims to explore precipitating and social risk factors for internet addiction (IA) in university undergraduate students, and to provide evidence for interventions and the early prevention of IA in different genders. Methods Four thousand eight hundred and fifty-eight college sophomores completed an online survey on their internet use-related behaviours and social risk factors. Results We found that more male (8.3%) than female students (5.4%) had moderate and severe IA. The main online activity in the moderate and severe IA groups was online gaming in males and online streaming in females. Roommates engaging in similar internetbased entertainment was a risk factor of IA only for males, while not being in a romantic relationship was a risk factor of IA for females only. Infatuation with the internet before college and adjustment problems for college life were shared risk factors for both genders in the mild and moderate IA groups. Conclusion IA was a common phenomenon in college students with shared and unique precipitating and social risk factors in males and females. The gender-sensitive risk factors for IA warranted earlier and individualized intervention and prevention strategies for IA in this population.
Jing-Han Chen,Chi-Te Liang,Chun-Feng Huang,Da-Ren Hang,David A. Ritchie,J. C. Hsiao,Jyun-Ying Lin,Michelle Y. Simmons,Ming-Gu Lin,S. H. Lo,Tsai-Yu Huang 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.50 No.3
By applying a magnetic field perpendicular to GaAs/AlGaAs two-dimensional electron systems (2DESs), we study the low-field Landau quantization when the thermal damping is reduced with decreasing temperature. Magneto-oscillations following the Shubnikov-de Haas (SdH) formula are observed even when their amplitudes are so large that the deviation to such a formula is expected. Our experimental results show the importance of the positive magneto-resistance to the extension of the SdH formula under the damping induced by the disorder.
Research on Health Crowdfunding: A Literature Review
Gu, Rui,Li, Jing,Xue, Tao 한국지능정보시스템학회 2022 한국지능정보시스템학회 학술대회논문집 Vol.2022 No.6
Health crowdfunding has become an important supplement to the health fundraising system and has attracted increasing attention from both researchers and practitioners. However, few efforts have been devoted to integrating the existing finding. This paper aimed to fill this gap. We conducted a systematic review of relevant literature and accordingly classified health crowdfunding into three types: health research crowdfunding, health innovation crowdfunding, and health expenses crowdfunding. Based on this classification. we reviewed the existing findings and discussed future research directions. This paper serves as a foundation for future research to conduct in-depth investigation and advances our understanding of health crowdfunding.
Safety Evaluation In Vitro of Enterococcus durans from Tibetan Traditional Fermented Yak Milk
Jing Li,Fazheng Ren,Huiyong Gu,Xiaopeng Li,Bozhong Gan 한국미생물학회 2011 The journal of microbiology Vol.49 No.5
Despite its ubiquity in fermented dairy products, the safety of lactic acid enterococcal bacteria remains controversial. In this study, five Enterococcus durans strains – A1, A2, B1, B2, and C1 – were isolated from traditional fermented yak milk from Tibet. To evaluate the strains’ safety, biogenic amine production, antibiotic resistance and presence of known virulence determinants were investigated. Strain A1 can produce biogenic amines for histamine, spermine, and spermidine (mean values: 8.64, 8.31, and 0.30 mg/L, respectively). Polymerase chain reaction amplification for Strain A1 found genes involved in expression of gelatinase (gleE), cytolysin (cylA, cylB, and cylM), sex pheromones (ccf and cpd) and cell wall adhesion (efaA). Strain A2 showed sensitivity or intermediate resistance to all tested antibiotics, and no virulence determinants except gelE and ccf, but did produce tyramine at a relatively high level (912.02 mg/L). Both strains B1 and B2 could produce histamine (10.43 and 10.56 mg/L, respectively), and showed vancomycin resistance; B1 also produced tyramine (504.02 mg/L). Strain C1 could produce all five biogenic amines tested in the study – putrescine, histamine, tyramine, spermine, and spermidine; concentrations were 6.51, 9.59, 205.85, 5.55, and 5.39 mg/L, respectively. All E. durans strains found in Tibetan traditional fermented yak milk thus offer potential risk.
Identification and Epigenetic Analysis of a Maternally Imprinted Gene Qpct
Jing Guo,Qiong Wu,Hongjuan He,Qi Liu,Fengwei Zhang,Jie Lv,Tiebo Zeng,Ning Gu 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.10
Most imprinted genes are concerned with embryonic development, especially placental development. Here, we identified a placenta-specific imprinted gene Qpct. Our results show that Qpct is widely expressed during early embryonic development and can be detected in the telecephalon, midbrain, and rhombencephalon at E9.5-E11.5. Moreover, Qpct is strikingly expressed in the brain, lung and liver in E15.5. Expression signals for Qpct achieved a peak at E15.5 during placental development and were only detected in the labyrinth layer in E15.5 placenta. ChIP assay results suggest that the modification of histone H3K4me3 can result in maternal activating of Qpct.
Niu-Jing Ma,Li-Xiong Gu,Chang-Li Peng 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.5
To make sure the tension adjustment in the cables of concrete cable-stayed bridges is conducted successfully, a new adjustment technique for the tension adjustment is presented, with the cable-girder temperature difference taken into account. The tensions are measured via frequency method. Prior to the tension adjustment, tensions and temperatures of all cables and girders are measured when the ambient temperature is stable, and this set of tensions is taken as reference tensions. During the tension adjustment, the relative variable quantities of the tensions are regarded as the control values, while the vertical displacement in the mid-span section of the main span is regarded as the verification value. After each pair of cables is adjusted, it is required to measure the temperatures of cables and girders as well as the vertical displacement variation in the mid-span section of the main span. To guarantee the structural behavior of entire bridge is controlled, at one-third cable points, two-third cable points and the farthest cable points, the frequencies of each pair of cables as well as the temperatures of each pair of cables and girders should be measured so as to recalculate the cable tensions. After that, these cable tensions are compared with the theoretical ones to ensure the structural behavior of entire bridge in control. Finally, Furong bridge, a concrete cable-stayed bridge with double inclined pylons, is taken as a sample application of this proposed technique. The results of cable tensions in this bridge illustrate the new adjustment technique is both accurate and efficient.