쌀보리 품종 및 도정률에 따른 항산화 및 Tyrosinase 저해 활성 평가

이석기(Seuk Ki Lee),우관식(Koan Sik Woo),이해림(Haelim Lee),이지혜(Jihae Lee),이병원(Byong Won Lee),이유영(Yu-Young Lee),이병규(Byoungkyu Lee),김선림(Sun Lim Kim),김현주(Hyun-Joo Kim) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.12

쌀보리를 식품 및 공중보건산업에서 기능성 소재로서의 활용가능성을 알아보고자 쌀보리 품종(새찰쌀, 흰찰쌀, 새쌀) 및 도정률(95%, 85%, 75%)에 따른 항산화 및 tyrosinase 저해 활성을 평가하였다. 각각의 시료를 70% 에탄올에 1:10의 비율로 48시간 추출하여 여과 후 동결건조 하여 실험에 사용하였다. 95% 도정률을 기준으로 한 쌀보리 추출물의 DPPH 라디칼 소거능이 각각 5.90, 4.63 및 17.57 mg TE/g sample로 나타났으며, FRAP 값을 측정한 결과 새쌀보리 추출물이 1,041.53 μM로 가장 높은 환원력을 가지는 것으로 확인되었다. 새찰쌀, 흰찰쌀 및 새쌀보리 추출물의 tyrosinase 저해 활성이 각각 30.64, 38.62 및 60.64%로 새쌀보리 추출물이 가장 높은 활성을 보였다. 총 폴리페놀 함량 측정 결과 새쌀보리 추출물은 172.51 μg GAE/g sample, 새찰쌀보리 48.87 μg GAE/g sample, 흰찰쌀보리 44.58 μg GAE/g sample로 높은 수치를 나타냈으며, 품종 및 도정률에 따른 플라보노이드 함량 측정 결과 총 폴리페놀 함량 결과와 경향이 동일하게 나타났다. 쌀보리의 도정률이 증가할수록 맥강에 함유되어 있는 폴리페놀 및 플라보노이드 등과 같은 기능성분이 제거됨에 따라 항산화 및 미백 등의 생리활성에 영향을 미친 것으로 판단된다. 이상의 결과를 종합하여 볼 때 쌀보리의 품종 및 도정률에 따라 항산화 및 tyrosinase 저해 활성의 차이를 보였으며, 쌀보리를 이용한 기능성 제품의 개발에 있어서 중요한 기초자료로 활용될 수 있을 것이라고 판단된다. The purpose of this study was to evaluate the effects of barley cultivars [Saechalssalbori (SC), Huinchalssalbori (H), and Saessalbori (S)] and different milling recovery rates (95%, 85%, and 75%) on antioxidant and tyrosinase inhibitory activities. Results show that the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of SC, H, and S extracts based on 95% coverage were 5.90, 4.63, and 17.57 mg Trolox equivalent/g sample, respectively. The ferric-reducing antioxidant potential (FRAP) value of S extract with 95% milling recovery was the highest at 1,041.53 μM. Tyrosinase inhibitory activities of SC, H, and S extracts were 30.64, 38.62, and 60.64%, respectively. Total polyphenol contents of SC, H, and S extracts based on 95% coverage were 48.87, 44.58, and 172.51 μg gallic acid equivalent/g sample, respectively. The total flavonoid content was highest in S extract with 95% milling recovery. The DPPH radical scavenging activity, FRAP value, tyrosinase inhibitory effect, total polyphenol, and flavonoid contents of barley extracts were reduced as milling recovery rate decreased. The results of this study suggest that milling recovery and cultivar of barley are important factors to improve the antioxidant and tyrosinase inhibitory activities of barley.

Impacts of the Atmospheric Interannual Variability on the West Antarctic Cryosphere

Jihae Kim,Myong-In Lee,Joonlee Lee,Nakbin Choi 한국기상학회 2019 한국기상학회 학술대회 논문집 Vol.2019 No.10

311-nm titanium:sapphire laser therapy for alopecia areata: a pilot study of 19 patients

( Jihae Lee ),( Ro Woo Lee ),( Soo Hyung Kim ),( Hyun Jeong Ju ),( Sung Hye Eun ),( Jung Min Bae ),( Gyong Moon Kim ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2

Background: Alopecia areata (AA) is an autoimmune skin disorder causing hair loss and has a large impact on patients’ quality of life. Laser treatment including 308-nm excimer laser has an advantage in treating AA in a targeted manner. Recently, a gain-switched 311-nm Titanium:Sapphire laser (TSL) was developed and demonstrated similar therapeutic efficacy to excimer laser in the treatment of vitiligo. Objectives: We evaluated the effectiveness and safety of the 311-nm TSL in the treatment of AA. Methods: We conducted an open trial and enrolled 19 AA patients between June 2017 and December 2018. A 311-nm TSL laser treatment was conducted once or twice a week. The dose started at 300 mJ/cm2 and increased by 50 mJ/cm2 in each subsequent session until post-treatment erythema occurred. Results: Among the 19 enrolled AA patients, three had alopecia totalis. The patients received a median of 14 sessions (range, 10-34) of TSL treatment for 3.5 months (range, 2-8). Fourteen patients (73.9%) showed excellent to complete (≥75%) hair regrowth. There were no serious adverse events to stop the treatment. Conclusion: The 311-nm TSL treatment has several advantages that it provides non-invasive, pain-free treatment to AA patients, without any risk of adverse drug reaction. In particular, TSL treatment has great benefits for children who worried about painful treatment.

P055 : Basal cell carcinomas with decreased immunoreactivity of Notch marker, a kind of subclinical field cancerization marker

( Jihae Lee ),( Jung Min Bae ),( Eu Seok Chung ),( Sae Mi Park ),( Ju Hee Lee ),( Hee Jin Jeon ),( Gyong Moon Kim ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: Basal carcinomas are well known skin malignancy and frequently associated with sun exposure that may be associated with subclinical cancerization. Objectives: We did research about the sunclinical cancerization marker, Notch, in cutaneous basal cell carcinomas. Methods: Recently surgically removed basal cell carcinomas were clinicopathologically reviewed, and Notch immunohistochemical stains were done. Results: Basal cell carcinomas showed much decreased expressions of Notch marker than normal areas. Assessment of Notch marker showed subclinical cancerization previously. Conclusion: Subclinical cancerization might be another related pathogenesis of basal cell carcinomas and further correlated pathways of basal cell carcinoma-Notch markers will be interesting for the perspectives of new topical anticancer therapeutics challenge.

A 24-hour acute fungicide ecotoxicity assessment method based on yeast (Kazachstania barnettii) growth

Seoha Lee,Sohyun Lee,Haneur Lee,Jihae Park,Taejun Han,Hojun Lee 환경독성보건학회 2023 한국독성학회 심포지움 및 학술발표회 Vol.2023 No.11

고혈압 및 당뇨병 환자의 건강관련 의사결정 참여경험

이지애(Jihae Lee) 한국콘텐츠학회 2021 한국콘텐츠학회논문지 Vol.21 No.3

본 연구의 목적은 고혈압 및 당뇨병 환자의 건강 관련 의사결정에 대한 참여경험을 탐색하는 것이다. 당뇨병 또는 고혈압의 만성질환을 진단받고 1년 이상 보건소에 정기적으로 방문하였던 장년기 환자 11명이 참여하였다. 자료의 수집은 개별 인터뷰를 통해 시행되었으며, Braun과 Clark 가 제시한 주제 분석법을 적용하여 분석되었다. 연구 결과 6개의 하위주제와 3개의 주제가 도출되었다. 각각의 주제는 ‘짧은 면담시간을 효율적으로 활용하기 위해 노력함’, ‘의료인에게 한정된 영역 안에서 응답함’, ‘건강관리에 대한 지속적인 동기부여가 결여됨’이었다. 본 연구 결과는 만성질환자의 의사결정에 대한 참여를 독려하기 위해 정확하고 충분한 건강정보를 제공하고 지지적인 태도로 환자의 동기를 부여해야 할 필요성을 제시하였다. This study attempted to explore the experience of patients’ having hypertension and diabetes participation in health care. 11 chronic patients who regularly visited community health centers for more than a year participated in this study. The data was collected through individual interviews and analyzed by thematic analysis method. As a result, 6 sub-themes and 3 themes derived from the data. The themes were ‘Trying to utilize short consultation time efficiently’, ‘Answering within narrow limits to healthcare provider’, ‘Being lack of continuous motivation for health care’. The result showed the need for nurses to provide accurate and sufficient health information and motivate patients in supportive manner to encourage chronic patients’ participation in health care.

직장암에 수술 전 항암화학방사선 동시 병용요법 후 종양의 병리학적 반응에 영향을 주는 임상적 예측 인자

이지혜(Jihae Lee),이경자(Kyung-Ja Lee) 대한방사선종양학회 2008 Radiation Oncology Journal Vol.26 No.4

목 적: 직장암에 수술 전 항암화학요법과 방사선치료를 동시에 병용시행한 후 완치절제술 후 병리학적 완전관해율 과 종양 병기 하향률에 영향을 주는 예측인자를 후향적으로 분석하여 환자에 따른 맞춤치료의 가능성을 알기 위하 여 본 연구를 시도하였다. 대상 및 방법: 2000년 5월부터 2008년 3월까지 국소적으로 진행된 직장암으로 진단받고 원격전이가 없는 환자39명을 대상으로 수술 전 항암화학요법과 골반강에 방사선치료 50.4 Gy (45∼59.4 Gy)를 시행한 후 완치적 수술후 절제된 조직을 병리학적으로 관찰하여 완전관해율과 종양 및 림프절의 병기하향률을 측정하였다. 항암화학요법은 39명 중 38명은 방사선치료 1주와 5주에 각각 1주간 5-fluorouracil과 leucovorin을 연속적으로 정맥 주입하였 으며 1명은 방사선치료기간 중 하루에 2회 capecitabine를 복용하였다. 결 과: 병리학적 완전관해를 보인 환자는 39명 중 12명(31%), 종양-병기하향을 보인 환자는 24명(63%)이었으며 임상적으로 림프절이 양성인 환자 28명 중 12명은(43%) 병리학적으로 하향되었으며 2명은 임상적으로 림프절이 음성이었으나 병리학적으로 양성을 보였다. 단변량분석에 의하면 종양의 둘레범위가 직장의 50% 미만(p=0.031),종양의 길이가 5 cm 미만(p=0.004), 치료 후 CEA 수치가 3.0 mg/mL 이하(p=0.015)인 환자에서 병리학적 완전관해율이 높았다. 또한 선암(p=0.045), 방사선량이 50 Gy 이상(p=0.021)인 환자에서 병기-하향률이 높았고, 방사선 치료 기간이 42일 이하인(p=0.018) 환자에서 림프절-병기하향률이 높았다. 다변량분석에 의하면 종양의 둘레범위가 50% 미만(HR 0.150; p=0.028), 종양의 길이가 5 cm 미만인(HR 0.084; p=0.005) 환자에서 통계학적으로 유의하게 병리학적 완전관해율이 높았으며, 방사선량이 50 Gy 이상 높은 경우(HR 0.115; p=0.025) 종양-병기하향률이높았고 방사선치료 기간이 42일 이하인(HR 0.028; p=0.010) 경우 림프절-병기하향률이 높았다. 결 론: 병리학적 완전관해에 영향을 주는 예측인자는 종양의 둘레범위와 종양의 길이이었으며, 종양-병리하향에 영향을 주는 예측인자는 방사선량, 림프절-병기하향에 영향을 주는 예측인자는 방사선치료 기간이었다. 이러한 예측인자를 파악함으로써 환자의 치료결과를 예측할 수 있으며 위험인자가 있는 환자에 좀 더 적극적인 치료계획을 설정하는 데 도움이 될 것으로 생각한다. Purpose: The objective of this retrospective study was to identify predictive factors for the complete pathologic response and tumor downstaging after preoperative concurrent chemoradiotherapy for locally advanced rectal cancer. Materials and Methods: Between the years 2000 and 2008, 39 patients with newly diagnosed rectal cancer without prior evidence of distant metastasis received preoperative concurrent chemoradiotherapy followed by surgery. The median radiation dose was 50.4 Gy (range, 45∼59.4 Gy). Thirty-eight patients received concurrent infusional 5-fluorouracil and leucovorin, while one patient received oral capecitabine twice daily during radiotherapy. Results: A complete pathologic response (CR) was demonstrated in 12 of 39 patients (31%), while T-downstaging was observed in 24 of 39 patients (63%). N-downstaging was observed in 18 of 28 patients (64%), with a positive node in the CT scan or ultrasound. Two patients with clinical negative nodes were observed in surgical specimens. The results from a univariate analysis indicated that the tumor circumferential extent was less than 50% (p=0.031). Moreover, the length of the tumor was less than 5 cm (p=0.004), while the post-treatment carcinoembryonic antigen (CEA) levels were less than or equal to 3.0 ng/mL (p=0.015) and were significantly associated with high pathologic CR rates. The univariate analysis also indicated that the adenocarcinoma (p=0.045) and radiation dose greater than or equal to 50 Gy (p=0.021) were significantly associated with high T-downstaging, while a radiotherapy duration of less than or equal to 42 days (p=0.018) was significantly associated with N-downstaging. The results from the multivariate analysis indicated that the lesser circumferential extent of the tumor (hazard ratio [HR], 0.150; p=0.028) and shorter tumor length (HR, 0.084; p=0.005) independently predicted a higher pathologic CR. The multivariate analysis also indicated that a higher radiation dose was significantly associated with higher T-downstaging (HR, 0.115; p=0.025), while the shorter duration of radiotherapy was significantly associated with higher N-downstaging (HR, 0.028; p=0.010). Conclusion: The circumferential extent of the tumor and its length was a predictor for the pathologic CR, while radiation dose and duration of radiotherapy were predictors for tumor downstaging. Hence, these factors may be used to predict outcomes for patients and to develop further treatment guidelines for high-risk patients.

임업분야 국제개발협력 전략 연구

이상민(Lee Sangmin),민경택(Min Kyungteak),구자춘(Koo Jachoon),이소영(Lee Soyoung),권지혜(Kwon Jihae) 한국농촌경제연구원 2015 한국농촌경제연구원 기본연구보고서 Vol.- No.-

MPLS 망에서 여러 병렬 LSP 존재시 트래픽 클래스의 성능목표를 고려하는 부하 균등화 방안

이지해(Jihae Lee),최명환(Myungwhan Choi) 한국통신학회 2001 한국통신학회 학술대회 및 강연회 Vol.2001 No.4

Reliability and Validity of the Beck Depression Inventory-II among Korean Adolescents

EunHo Lee,SooJi Lee,SoonTaeg Hwang,SangHwang Hong,JiHae Kim 대한신경정신의학회 2017 PSYCHIATRY INVESTIGATION Vol.14 No.1

Objective-The Beck Depression Inventory-II (BDI-II) is one of the most popular scales for evaluating the severity of depression in adolescents as well as adults. The prevalence of depression increases during adolescence, and it has shown a rapid increase with occurrence at an earlier age and a tendency to continue into adulthood. Data from an adolescent nonclinical sample provides us more information related to depressive symptoms as potential risk factors. The current study was designed to two objectives: 1) to analyze the reliability and validity the BDI-II among Korean adolescents and 2) to evaluate the factorial structure in a Korean nonclinical adolescent sample. Methods-The participants included 1072 adolescent boys and girls. We assessed the internal consistency, corrected item-total correlation, and the convergent validity of the BDI-II. We also performed confirmatory factor analyses to determine the internal structure of the BDI-II for Korean adolescents using Mplus 6.1. Results-The Cronbach’s alpha for the BDI-II total score was 0.89. The correlation between the BDI-II and the PHQ-9 was strong (r=0.75), and anxiety-related measures were 0.68 and 0.71, which were also in the high range. Among the five different factor structures, the modified three-factor model demonstrated the best overall fit. Conclusion-The BDI-II is a reliable tool for measuring the severity of depressive symptoms in Korean adolescents. Therefore, the findings can provide basic information for examining the prevalence rate, intervention strategies for depression in adolescents.