Molecular cloning, chromosomal localization and expression profiling of porcine selenoprotein M gene

Ji-Chang Zhou,Hua Zhao,Jia-Yong Tang,Jun-Gang Li,Xiao-Li Liu,Yu-Mei Zhu 한국유전학회 2011 Genes & Genomics Vol.33 No.5

Selenoprotein M may regulate a myriad of biological processes through its redox function. In pigs, neither the nucleotide sequence nor the amino acid sequence is known. Furthermore,patterns of tissue expression and regulation by dietary selenium (Se) have not been examined. We determined the full coding sequence (CDS) and the chromosomal location of the porcine gene, SELM, and described its expression profile in vivo under different dietary Se concentrations. The cDNA sequence of porcine SELM from the start codon to the poly(A) tail was cloned by reverse transcription PCR. The CDS contained 429bases with a typical mammalian selenocysteine insertion sequence of form 2 (F2) located in the 3′-untranslated region. The gene was mapped to chromosome 14q21, where porcine SELM and its neighboring genes exhibited a similar organization to human homologues on chromosome 22q12.2. The expression pattern of SELM mRNA in muscle, thyroid, cerebral cortex, pituitary, testis, liver, and kidney was analyzed with real-time quantitative PCR in young male pigs fed a Se-deficient corn-soybean meal basal diet supplemented with 0.0, 0.3,or 3.0 mg Se/kg in the form of Se-rich yeast. Though the SELM mRNA abundance in each of the 7 tissues was not affected by the dietary Se concentrations, it was significantly higher in thyroid (P < 0.01) than in cerebral cortex, pituitary,testis, liver, and kidney at all of the 3 dietary Se concentrations.

A Comparison of Odor Intensity Perception by Chinese and South Koreans

( Ik-jun Yeon ),( Yi-xuan Zhao ),( Ju-yong Jung ),( Sung-sik Park ),( Ji-sun Jung ) 한국환경기술학회 2019 한국환경기술학회지 Vol.20 No.5

Several industrial complexes have been established in South Korea and China during their industrialization and economic development; at the same time, the odors released from these industrial complexes caused popular discontent among neighboring residents. In this study, residents living in the industrial complexes in South Korea and China were selected as subjects of an empirical analysis on the cognition degree of odor; In particular, we analyzed the factors affecting odor intensity. Our study was based on the responses of 127 Chinese and 152 South Korean people living near the Nanjing and Ochang industrial parks, respectively. The results revealed that the perception of Chinese and South Korean residents was affected by different sets of factors. In the former case, the major factors in order of importance were ‘Danger awareness’, ‘Economic loss’, and ‘Trust’; in the latter case, they were instead ‘Danger awareness’, ‘Development’, ‘Economic benefit’, and ‘Trust’. A multiple regression analysis demonstrated that odor perception in both Chinese and South Korean residents was accentuated if they possessed an environmental ‘Danger awareness’. Additional regression analyses showed that the odor experience in itself had no significant impacts on the resident's awareness of odor intensity in South Korea, while it had significant effects in China.

제조 공장의 생산물류 평가 프레임워크에 관한 연구

권지선(Ji Sun Kwon),전찬모(Chan Mo Jun),조문빈(Wen Bin Zhao),노상도(Sang Do Noh) (사)한국CDE학회 2014 한국 CAD/CAM 학회 학술발표회 논문집 Vol.2014 No.2

Manufacturing industries are endeavoring to gain production competitiveness by developing various production strategies or by applying production forms based on advanced technologies. Among them, the assessment of optimized production logistics planning and application of it to reduce the cost incurred during flow of materials is significantly considered by industries. Based on importance of these assessments, this research proposes a framework and a model for assessment of production logistics by drawing quantitative results to classify by important elements of production logistics into ‘Flow’, ‘Area’ and ‘Material Handling Equipment’ from 4 different points of view to assess each of elements objectively and by bringing quantitative way of assessing after defining assessment index system for each element to derive assessment element of production logistics. . This research ultimately aims at supporting industries to make reasonable decisions by presenting objective framework and model for assessment of production logistics for planning and optimization of it to enable understanding and comparison of current level of production logistics and foundation of strategic approach to the point of improvement or future planning of production logistics.

Salivary Pepsin as an Intrinsic Marker for Diagnosis of Sub-types of Gastroesophageal Reflux Disease and Gastroesophageal Reflux Disease-related Disorders

( Yan-jun Wang ),( Xiu-qiong Lang ),( Dan Wu ),( Yu-qin He ),( Chun-hui Lan ),( Xiao-xiao ),( Bin Wang ),( Duo-wu Zou ),( Ji-min Wu ),( Yong-bin Zhao ),( Peter W Dettmar ),( Dong-feng Chen ),( Min Yan 대한소화기 기능성질환·운동학회 2020 Journal of Neurogastroenterology and Motility (JNM Vol.26 No.1

Background/Aims To determine the value of salivary pepsin in discriminating sub-types of gastroesophageal reflux disease (GERD) and GERD-related disorders. Methods Overall, 322 patients with different sub-types of GERD and 45 healthy controls (HC) were studied. All patients took Gastroesophageal Reflux Disease Questionnaire (GerdQ) and underwent endoscopy and 24-hour esophageal pH monitoring and manometry. Salivary pepsin concentration (SPC) was detected by using colloidal gold double-antibody immunological sandwich assay. Oral esomeprazole treatment was administrated in the patients with non-erosive reflux disease (NERD) and extra-esophageal symptoms (EES). Results Compared to HC, patients with erosive esophagitis, NERD, EES, EES plus typical GERD symptoms, or Barrett’s esophagus had a higher prevalence of saliva and SPC (all P < 0.001). There was no significant difference in the positive rate for pepsin in patients with functional heartburn or GERD with anxiety and depression, compared to HC. After esomeprazole treatment, the positive rate and SPC were significantly reduced in NERD (both P < 0.001) and in EES (P = 0.001 and P = 0.002, respectively). Of the 64 NERD patients, 71.9% (n = 46) were positive for salivary pepsin, which was significantly higher than the rate (43.8%, n = 28) of pathological acid reflux as detected by 24-hour esophageal pH monitoring (P = 0.002). Conclusions Salivary pepsin has an important significance for the diagnosis of GERD and GERD-related disorders. Salivary pepsin and 24-hour esophageal pH monitoring may complement with each other to improve the diagnostic efficiency.

충주시 생활폐기물의 성상특성 변화

정지선 ( Ji-sun Jung ),조의훤 ( Yixuan Zhao ),유민지 ( Min-ji Yu ),최원회 ( Won-hoe Choi ),연익준 ( Ik-jun Yeon ) 한국폐기물자원순환학회(구 한국폐기물학회) 2017 한국폐기물자원순환학회 춘계학술발표논문집 Vol.2017 No.-

우리나라의 폐기물 발생량은 인구증가, 경제성장, 삶의 질 향상에 의해 꾸준히 증가하고 있으며, 증가하는 폐기물에 ‘자원화’로 인식을 전환시켜 재사용, 재활용하기 위한 다양한 정책을 추진중에 있다. 또한 자원화 방법 외에 소각 등의 처리를 한 후 매립장에 매립하고 있다. 자원화 방법 외에 소각과 매립의 방법으로 발생되는 폐기물을 적정하게 처리하기 위해서는 환경기초시설의 설치가 필수적이고, 환경기초시설(매립시설, 소각시설)의 설계 및 용량을 결정하기 위해서는 발생되고 있는 폐기물에 대한 정확한 분석과정이 우선시 되어야 하며, 이 과정은 매우 중요하다고 할 수 있다. 더욱이 소각시설의 경우 대기오염, 악취, 자원에 대한 낭비 등의 문제를 야기할 가능성이 높기 때문에, 소각시설의 적정 운전을 위하여 폐기물에 대한 물리·화학적 분석과 발열량에 대한 기초자료는 소각시설 설계 시 매우 중요 인자로 활용되고 있다. 하지만 소각시설 설계 시 고려되었던 폐기물에 대한 기초 자료로 설정한 소각시설의 운전조건이 실제로 소각시설이 운영되었을 때를 얼마만큼 유지하고 있는지는 지속적인 조사가 필요할 것이다. 왜냐하면 시간이 지나면서 사람들의 소비패턴이 변하며, 발생되는 폐기물의 양과 성분도 변하기 때문이다. 이에 본 연구에서는 인구 22만 명 정도의 중소도시를 대상으로 소각시설이 운영되고 있는 지역을 선정하였고, 선정된 지역의 과거부터 현재까지의 발생되고 있는 생활폐기물의 특성을 분석하고자 하였다. 생활폐기물의 변화특성을 조사·분석하기 위하여, 겉보기밀도, 삼성분분석, 발열량분석 등을 수행하였다. 향후 이 자료는 충주시에서 소각시설 운영을 위한 최적의 운전인자를 도출하는데 중요 자료로 활용될 수 있으며, 폐기물관리 정책수립 및 처리에 기초자료로 제시하고자 한다.

Factors Potentially Associated with Chemotherapy-induced Anemia in Patients with Solid Cancers

Cheng, Ke,Zhao, Feng,Gao, Feng,Dong, Hang,Men, Hai-Tao,Chen, Ye,Li, Long-Hao,Ge, Jun,Tang, Jie,Ding, Jing,Chen, Xin,Du, Yang,Luo, Wu-Xia,Liu, Ji-Yan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Purpose: Chemotherapy-induced anemia (CIA) is one of the most important causes of anemia in cancer patients. This study was conducted to describe the prevalence and characteristics of CIA in solid cancer patients in the Chinese population, and to explore the relationship of white blood cell (WBC) or platelet decrease with CIA. Methods: Data on age, gender, tumor diagnosis, anti-cancer treatment and blood cell analyses were available from 220 untreated non-anemic cancer patients who received at least 2 cycles of chemotherapy, and the data were analyzed to assess their relationship with CIA or its severity. Results: 139 patients (63.2%) presented anemia, most being Grade 1 or 2. Esophageal and lung cancers were associated with a high prevalence. G3/4 leucopenia and decrease of platelets were identified as independent risk factors for the occurrence of CIA. Moreover, G3/4 leucopenia, decrease of platelet and G3/4 thrombocytopenia were found to be also associated with the severity of CIA. Cisplatin-containing regimens were a main potential factor in causing CIA, although significant association was only found on univariate analysis. Conclusion: Anemia or decrease in hematoglobin are common in Chinese cancer patients receiving chemotherapy. Cisplatin-containing regimens might be an important factor influencing the occurrence of CIA. Our analysis firstly described some risk factors, such as decrease of platelets or WBCs, severity of leucopenia or thrombocytopenia, associated with the occurrence and severity of CIA.

Molecular Mechanisms of Casticin Action: an Update on its Antitumor Functions

Rasul, Azhar,Zhao, Bin-Ji,Liu, Jun,Liu, Bao,Sun, Jia-Xin,Li, Jiang,Li, Xiao-Meng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Casticin (3', 5-dihydroxy-3, 4', 6, 7-tetramethoxyflavone) is an active compound isolated from roots, stems, leaves, fruits and seeds of a variety of plants. It is well known for its pharmacological properties and has been utilized as an anti-hyperprolactinemia, anti-tumor, anti-inflammatory, neuroprotetective, analgesic and immunomodulatory agent. Recently, the anticancer activity of casticin has been extensively investigated. The resulkts showed that it exerts protective potential by targeting apoptosis, considered important for cancer therapies. In this article, our aim was to review the pharmacological and therapeutic applications of casticin with specific emphasis on its anticancer functions and related molecular mechanisms. Chemotherapeutic effects are dependent on multiple molecular pathways, which may provide a new perspective of casticin as a candidate anti-neoplastic drug. This review suggests that additional studies and preclinical trials are required to determine specific intracellular sites of action and derivative targets in order to fully understand the mechanisms of its antitumor activity and validate this compound as a medicinal agent for the prevention and treatment of various cancers.

Electronic Structure, Phase Transition, and Elastic Properties of ScC under High Pressure

Yu-Xin Zhao,Jun Zhu,Yan-Jun Hao,Zi-Yuan Li,Long-Qing Chen,Guang-Fu Ji 한국물리학회 2015 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.67 No.12

The structural properties and the phase transition for scandium carbide (ScC) have been studied in NaCl (B1), CsCl (B2), ZB (B3), WZ (B4), NiAs (B81), WC (Bh), and Pmmn structures by using the pseudopotential plane-wave method in the framework of the density functional theory. Our theoretical results show that the most stable structure is the B1 phase, contrary to the result of Rahim et al. The phase transitions B1 ! Pmmn and Pmmn ! B2 are predicted at 83.7 and 109.7 GPa, respectively. At the same time, we find that the B3, B4, B81, and Bh phases are not stable over the whole pressure range considered. In particular, the elastic constants of Pmmn-ScC under high pressure are obtained successfully. The effects of pressure on the elastic properties of B1-ScC and Pmmn-ScC are also predicted. The Debye temperatures and the sound velocities of these two structures are estimated from the elastic constants, and by analyzing G/B, the brittle-ductile behavior of ScC is assessed. In addition, the density of states of B1-ScC at high pressures is also discussed.

Population dynamics, hunting nature on insect pests and existence of symbiotic bacterial microbes among leading transgenic cotton spiders

Nasir Muhammad,Zhao Chen-chen,Luo Jun-yu,Zhu Xiang-zhen,Gao Xue-ke,Iqbal Mazher F.,Ji Ji-chao,Zhang Shuai,Cui Jin-jie 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1

The genetically modified cotton holding Bt proteins, is noxious to bollworms larvae but very little is known about its impact on population of non-target spiders within the field. Studies conducted with the aim to identify spiders along with their abundance, prevalence pattern, preying habit on insect pests in Bt cotton (L280, Cry2Ab4) and commonness of symbiotic bacteria in these spiders. Spiders collections were made via vial tapping/jarring, from May to end of October (2018). In total, 13,342 spiders were recorded during the entire cropping season. Least relative abundance was in May (0.28%), highest in August (30.39%) while ahead decline was observed, reached to 12% in October. The 27 species were verified, least richness was in May (3 species), high in August (19 species), later in the end, reduced to 7 species. High abundance was of Hylyphantes graminicola (68.56%) and Neoscona theisi (19.98%). Existence of H. graminicola and Pardosa astrigera observed during the whole cropping season. Presence of seven guilds among spiders had a diverse nature of hunting insect pests and on number of species existed in the crop, maximum was from direct hunting habit. Composition and community structure of microbiota varied as of spider species. Proteobacteria was the most abundant bacterial phylum and topmost genus was Wolbachia in observed spiders. Five groups of bacteria distinguished across 4 clusters of spiders depending on core community of symbiotic bacterial genera. This effort is an initial step to get knowhow about spiders in Bt cotton, which will facilitate future research studies concerning spiders.

A New Spongilipid from the Freshwater Sponge Spongilla lacustris

Hu, Jiang-Miao,Zhao, You-Xing,Chen, Ji-Jun,Miao, Ze-Hong,Zhou, Jun Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.5