Ampelopsis japonica Makino extract (AE) inhibits the inflammatory reaction induced by pathogen-associated molecular patterns (PAMPs) in epidermal keratinocytes

( Mi Ra Choi ),( Jin Hyup Lee ),( Dae Kyoung Choi ),( Dong Il Kim ),( Hae Eul Lee ),( Myung Im ),( Young Lee ),( Chang Deok Kim ),( Young Joon Seo ),( Jeung Hoon Lee ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Keratinocytes are the major cells inepidermis, providing barrier components such as cornified cells through the sophisticated differentiation process. In addition, keratinocytes exerts their role as the defense cells via activation of innate immunity. It has been knownthat pathogen-associated molecular patterns (PAMPs) including double-strand RNA and nucleotides can provoke inflammatory reaction in keratinocytes. Objectives: The aim of this study is to evaluate the effect of Ampelopsis japonica Makino extract (AE) on PAMPs-induced inflammatory reaction of keratinocytes. Methods: The effects of AE were determined using poly(I:C)-induced inflammation and imiquimod-induced psoriasiform dermatitis models. Results: In cultured keratinocytes, AE significantly inhibited poly(I:C)-induced expression of inflammatory cytokines, such as IL-1モ, IL-6, IL-8 and TNF-メ. AE significantly inhibited poly(I:C)-induced release of caspase-1 active form (p20), and down-regulated NF-リB signaling pathway. In imiquimod-induced psoriasiform dermatitis model, topical application of AE resulted in significant reduction of epidermal hyperplasia. Conclusion: These results suggest that AE may be a potential candidate for the treatment of skin inflammation.

Ampelopsis japonica Makino Extract Inhibits the Inflammatory Reaction Induced by Pathogen-Associated Molecular Patterns in Epidermal Keratinocytes

( Mi Ra Choi ),( Dae Kyoung Choi ),( Ki Duck Kim ),( Sue Jeong Kim ),( Dong Il Kim ),( Myung Im ),( Young Lee ),( Young Joon Seo ),( Chang Deok Kim ),( Jeung Hoon Lee ) 대한피부과학회 2016 Annals of Dermatology Vol.28 No.3

Background: Keratinocytes are the major cells in epidermis, providing barrier components such as cornified cells through the sophisticated differentiation process. In addition, keratinocytes exerts their role as the defense cells via activation of innate immunity. It has been known that pathogen-associated molecular patterns (PAMPs) including double-strand RNA and nucleotides can provoke inflammatory reaction in keratinocytes. Objective: The aim of this study is to evaluate the effect of Ampelopsis japonica Makino extract (AE) on PAMPs-induced inflammatory reaction of keratinocytes. Methods: The effects of AE were determined using poly (I:C)-induced inflammation and imiquimod-induced psoriasiform dermatitis models. Results: In cultured keratinocytes, AE significantly inhibited poly(I:C)-induced expression of inflammatory cytokines, such as interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor-α. AE significantly inhibited poly(I:C)-induced release of caspase-1 active form (p20), and down-regulated nuclear factor-κB signaling pathway. In imiquimod- induced psoriasiform dermatitis model, topical application of AE resulted in significant reduction of epidermal hyperplasia. Conclusion: These results suggest that AE may be a potential candidate for the treatment of skin inflam-mation. (Ann Dermatol 28(3) 352∼359, 2016)

외상 후 뇌 지방색전증 1예

문정미,소정일,김용권,류진호,허탁,서정진,민용일 대한응급의학회 2001 대한응급의학회지 Vol.12 No.2

Post-traumatic fat embolism was first reported by Zenker in 1862, Von Bergmann reported the first clinical diagnosis of the fat embolism syndrome in 1873. Fat embolism has been associated with traumatic or non-traumatic disorders. Fat embolization after long bone trauma is probably common as a subclinical event. The diagnosis of fat embolism syndrome is based on the patient's history, is supported by clinical signs of pulmonary, cerebral, and cutaneous dysfunction, and is confirmed by the demonstration of arterial hypoxemia in the abscence of other disorders. Two different mechanisms cause fat to embolize: direct entry of deposit fat into the blood stream and agglutination of endogenous or exogenous plasma fat. MRI can detect a cerebral fat embolism with a higher sensitivity than cerebral CT. We report a case of post-traumatic cerebral fat embolism without pulmonary involvement, and we present a review of the literature. A 16-year girl had a traffic accident and pelvic bone fracture. Forty eight hours later severe trauma become stuporous without a focal neurological deficit. The patient received supportive therapy, and her condition improved throughout her hospital course. She was discharged with good condition after a 30-day hospital stay.

단일전극을 가진 마이크로 가스센서의 제작 및 특성

송갑득,박영일,이상문,이윤수,최낙진,주병수,서무교,허증수,이덕동 한국센서학회 2002 센서학회지 Vol.11 No.6

센서의 안정도와 감도를 개선시킬 수 있는 단일전극을 가진 열선형 마이크로 가스센서를 제작하였다. 일반적으로, 금속산화물 반도체를 이용한 가스센서는 히터전극과 감지전극의 두 개의 전극을 가지고 있다. 제작된 센서는 히터전극위에 감지물질을 형성하여 단일 전극을 가지는 구조를 가지고 있다. 히터와 감지전극으로 사용되는 Pt는 glass 기판위에 스퍼터링법으로 형성하였으며, SnO_2 감지물질은 제작된 Pt 전극위에 열증착시켜 형성하였다. SnO_2막은 lift-off 공정을 이용해서 패턴을 형성하고 1시간 동안 산소분위기에서 열산화하였다. 제작된 소자의 크기는 1.92x.1 mm^2 이다. CO 가스에 대한 감지특성을 조사한 결과 1,000 ppm에 대해 100 mV의 출력변화를 나타내었으며, 넓은 농도범위(0 ~ 10,000 ppm에서 선형적인 전압출력을 나타내었다. 또한 가스 반응 전과 반응 후의 전압출력을 비교해 볼 때, 1 % 이내의 편차를 나타내는 우수한 회복성을 나타내었다. Micro gas sensor with single electrode was proposed for improving stability and sensitivity, Generally, metal oxide gas sensors have two electrodes for heating and sensing. This fabricated new type sensor have only a single electrode by forming a sensing material onto heating electrode. Pt as a heating and sensing electrode was sputtered on glass and a SnO_2 sensing material was thermally evaporated on Pt electrode. SnO_2 was patterned by lift-off process and then thermally oxidized in O_2 condition for 1 hr., 600 ℃. The size fabricated sensor was 1.9 x 2.1 mm^2. As a result of CO gas sensing characteristics, this sensor showed 100 mV change for 1,000 ppm and linearlity for wide range(0 ~ 10,000 ppm) of gas concentrations. And the sensor shows a good recovery characteristics of 1 % deviation compared to initial resistance.

Adapting a Novel Dual Authentication Method based on Smart Cards

Jeung-Seop Kim1,Jong-Il Kim,Chang-Jin Seo,Yong-Seok Jang 보안공학연구지원센터 2013 International Journal of Smart Home Vol.7 No.5

세포학적 검시기준에 대한 재고찰 : 폐질환의 세포병리학적 연구

서정일,문영천,박효숙 최신의학사 1981 最新醫學 Vol.24 No.1

선천적 배측 췌장 부분 발육 부전증 1 예

김일두,김수형,서길동,김명준,허정호,조성락 대한소화기내시경학회 2001 Clinical Endoscopy Vol.23 No.3

Complete or partial agenesis of dorsal pancreas has been reported in a small number of pediatric and adult patients. A case of partial agenesis of dorsal pancreas was reported. This case was not associated with diabetes mellitus and pancreatic exocrine dysfunction, or abdominal pain. A 37-year-old man with chronic hepatitis B visited to our hospital due to further examination for chronic hepatitis B. Abdominal ultrasonography showed enlarged pancreatic head. Diagnosis was made by endoscopic retrograde cholangiopancreatography and computed tomography. Explorlaparotomy was not done.

고온 수용액으로부터의 고품질 α-Quartz 분말 제조

목영일,이기정,서경원,유효신 한국화학공학회 1997 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.35 No.2

입자의 크기가 균일하고 양질의 결정성을 갖는 고품질 α-SiO₂단결정 분말을 수열 조건에서 제조하였다. α-SiO₂ 단결정 분말은 300-450℃의 온도 범위와 100-300atm의 압력 범위내에서 3시간 동안의 수열반응을 통해 제조되었다. 반응은 무정형 실리카를 영양제로 사용한 성장용액의 용해도를 증가시키기 위해 KOH, NaOH. Na₂CO₃, KF, NaF와 같은 알칼리와 할로겐 화합물을 광화제로 첨가해서 수열합성시켰으며, 비교적 소량의 첨가에도 우수한 효과를 나타냈다. 수열합성법을 통해 입도가 1-10㎛ 범위내에 해당하고, 육방정의 안정된 결정형을 갖는 α-SiO₂ 단결정 분말을 얻을 수 있었다. XRD, SEM, PSA, FT-IR과 Raman spectrometer를 이용해서 수열합성물의 물성 특성을 비교 분석하였다. In this study powders of high quality α-quartz(α-SiO₂) crystal having uniform particle sizes und high crystallinity were prepared under hydrothermal conditions. The α-quartz powders were prepared after a 3 hours' operation in the temperature range of 300-450℃ and in the pressure range of 100-300atm. Alkali hydroxides and alkali halides such as KOH, NaOH. Na₂CO₃, KF and NaF were found to be effective in this reactions, which added in order to increase the solubility of amorphous silica feedstock. The α-quartz crystal powders were obtained in uniform size of 1-10㎛ with hexagonal structure. The characterization of the synthetic products were carried out using XRD, SEM, PSA, FTIR and Raman spectrometer.

THE STUDY ON THE EFFECTIVE FACTORS OF HYDROTHERMAL SYNTHESIS IN PREPARING HIGH QUALITY CRYSTALLINE α - QUARTZ POWDERS

Lee, Kee Jeung,Seo, Kyung Won,Yu, Hyo Shin,Mok, Young Il 한국화학공학회 1996 Korean Journal of Chemical Engineering Vol.13 No.5

In this study powders of high quality crystalline α-quartz were prepared by hydrothermal synthesis and parameters related to the preparation of the material were investigated. The degree of face development of the α-quartz crystal depended on the relative growth rates of a particular form that varies considerably with the degree of supersaturation. reaction temperature, concentration of mineralizers, and the amount of seed crystals in the feedstock. In the temperature range of 240-450℃ and the pressure range of 700-300 atm, alkali hydroxides and alkali halides such as KOH, NaOH, Na₂CO₃, KF, and NaF were found to he the effective mineralizers. As the concentration of mineralizers increased, the particle size of crystalline α-quartz powders became smaller at lower reaction temperatures. The fractional size distribution of synthetic crystal powders depended on the hydrothermal conditions. As the reaction time and the amount of seed crystals increased, the width of particle size distribution became narrower. The weight mean particle size was in the range of 1 ㎛ to 10 ㎛. The characterization of the products were carried out using XRD, SEM, PSA, FTIR, and Raman spectrometer.

군대에서 발생한 봉소염 환자의 임상 양상에 관한 연구

서구일(Koo Il Seo),조광현(Kwang Hyun Cho),김계정(Kea Jeung Kim),은희철(Hee Chul Eun) 대한피부과학회 1999 대한피부과학회지 Vol.37 No.9

N/A Background : Cellulitis is one of the most common infectious skin diseases in primary care units of military service in which personal hygiene could be easily neglected under special circumstances such as outdoor training. Objective : This study was designed to elucidate the probable causes of the high incidence rate of cellulitis in military service. Methods : We prospectively evaluated the clinical findings of 59 patients with cellulitis who were hospitalized in the medical detachment of the 1st Infantry Division of the ROK Army from May, 1995 to April, 1996. Results : The mean age of patients was 21(19-25). Analysing the rank of the patients, the number of recruits was 3(5%), private 26(44%), private first class 17(29%), corporal 11(19%), and sergeant 2(3%). Seventy eight percent of them were included in ranks below corporal. The recurrence rate of cellulitis during the 2 1/2 year follow-up period was only 4%. Skin lesions noted were erythema(100%), tendemess(98%), swelling(98%), local heating(97%), lymphadenopathy(73%), pus(63%), fluctuation(32%), ulcer(27%), lymphangitis(14%), bullae(8%), etc. The most common route of infection was the previous wounds(46%) which allows a portal for inbasion of microorganisms. Tinea pedis was also found to be in 39% of patients with cellulitis of the lower leg. The pus culture performed on 7 patients who had not been responsive to primary antibiotics resulted in 6 positive cases of Staphylococcus aureus. Conclusion : Considering the ranks of the patients and the routes of infection, we found that the incidence of cellulitis in the military service of the ROK Army could be reduced if more attention would be paid to preventive counterplans for secondary infection and tinea pedis. (Korean J Dermatol 1999;37(9) : 1305∼1309)