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Lee, Seong-Kon,Kim, Beom-Gi,Kwon, Taek-Ryoun,Jeong, Mi-Jeong,Park, Sang-Ryeol,Lee, Jung-Won,Byun, Myung-Ok,Kwon, Hawk-Bin,Matthews, Benjamin F,Hong, Choo-Bong,Park, Soo-Chul Indian Academy of Sciences 2011 Journal of biosciences Vol.36 No.1
<P>Mitogen-activated protein kinases (MAPK) signalling cascades are activated by extracellular stimuli such as environmental stresses and pathogens in higher eukaryotic plants. To know more about MAPK signalling in plants, aMAPK cDNA clone, OsMAPK33, was isolated from rice. The gene is mainly induced by drought stress. In phylogenetic analysis, OsMAPK33 (Os02g0148100) showed approximately 47-93% identity at the amino acid level with other plant MAPKs. It was found to exhibit organ-specific expression with relatively higher expression in leaves as compared with roots or stems, and to exist as a single copy in the rice genome. To investigate the biological functions of OsMAPK33 in rice MAPK signalling, transgenic rice plants that either overexpressed or suppressed OsMAPK33 were made. Under dehydration conditions, the suppressed lines showed lower osmotic potential compared with that of wild-type plants, suggesting a role of OsMAPK33 in osmotic homeostasis. Nonetheless, the suppressed lines did not display any significant difference in drought tolerance compared with their wild-type plants. With increased salinity, there was still no difference in salt tolerance between OsMAPK33-suppressed lines and their wild-type plants. However, the overexpressing lines showed greater reduction in biomass accumulation and higher sodium uptake into cells, resulting in a lower K+/Na+ ratio inside the cell than that in the wild-type plants and OsMAPK33-suppressed lines. These results suggest that OsMAPK33 could play a negative role in salt tolerance through unfavourable ion homeostasis. Gene expression profiling of OsMAPK33 transgenic lines through rice DNA chip analysis showed that OsMAPK33 altered expression of genes involved in ion transport. Further characterization of downstream components will elucidate various biological functions of this novel rice MAPK.</P>
Kim, Dool-Yi,Kwon, Hawk-Bin,Lee, Hye-Eun,Jeong, Mi-Jeong,Go, Seung-Joo,Byun, Myung-Ok 한국유전학회 2004 Genes & Genomics Vol.26 No.3
Changes of expression in cold-regulated mRNA levels in potato (Solanum tuberosum L. cv. Superior) were analyzed. A total of 12,000 cDNAs was subjected to reverse Northern blot analysis using ^(32)P-dCTP- labeled first strand cDNA generated from the total RNA isolated from 25℃- and 4℃-treated potato plants. A total of 245 cDNA clones were sequenced from a cDNA library constructed from the cold treatment. Based on the BLAST results, 103 cDNA clones were found to be redundant. The analyzed genes were classified into 12 groups according to their putative functions, where the 20.2 % of group I was associated with energy metabolism, 13.1% of group XI with cell rescue and defense, 2.6% of group Ⅷwith signal transduction. Among the cDNA clones up-regulated by cold treatment from the results of the reverse Northern blot analysis, 32 were used for the Northern blot analysis. Most of the cold-treated clones showed overexpression compared with the control, while some showed down-regulation. In general, it was found that cold stress related genes were overexpressed more than two-folds at 4℃ treatment.
Sang Hyuk Park,Hyun Ji Lee,In-Suk Kim,Jeong-Eun Kang,Eun Yup Lee,Hyeoung-Joon Kim,Yeo-Kyeoung Kim,Jong-Ho Won,Soo-Mee Bang,Hawk Kim,Moo-Kon Song,Joo Seop Chung,Ho Jin Shin 대한진단검사의학회 2015 Annals of Laboratory Medicine Vol.35 No.3
Background: To identify potential molecular prognostic markers in core binding factor (CBF) AML, we analyzed incidences and prognostic impacts of mutations in c-KIT, WT1, CEBPA, CBL, and a number of epigenetic genes in CBF AML. Methods: Seventy one and 21 AML patients with t(8;21) and inv(16) were enrolled in this study, respectively. NPM1, CEBPA, c-KIT, IDH1/2, DNMT3A, EZH2, WT1, and CBL mutations were analyzed by direct sequencing. Patients were categorized with respect to c-KIT and WT1 mutation status, and both clinical features and prognoses were compared. Results: The incidences of FLT3 internal tandem duplication (ITD), NPM1, CEBPA, IDH1/2, DNMT3A, EZH2, and CBL mutations were low (≤5%) in CBF AML patients. However, c-KIT and WT1 mutations occurred frequently (10.9% and 13.8%, respectively). t(8;21) patients with c-KIT mutations showed significantly shorter overall survival (OS) and disease free survival (DFS) periods than those without mutations (P<0.001, for both); however, although the limited number of t(8;21) patients were analyzed, WT1 mutation status did not affect prognosis significantly. Relapse or death during follow-up occurred more frequently in t(8;21) patients carrying c-KIT mutations than in those without the mutation, although the difference was significant only in a specific patient subgroup with no WT1 mutations (P=0.014). Conclusions: The incidences of mutations in epigenetic genes are very low in CBF AML; however, c-KIT and WT1 mutations occur more frequently than others. The poor prognostic impact of c-KIT mutation in t(8;21) AML patients only applies in a specific patient subgroup without WT1 mutations. The prognostic impact of WT1 mutation in CBF AML is not evident and further investigation is required.